Krishanti, Ni Putu Ratna Ayu
Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Phylogenetic Analysis of Polyporous Fungi Collected from Batam Botanical Garden, Riau Province, Indonesia Lestari, Anis Sri; Zulfiana, Deni; Zulfitri, Apriwi; Krishanti, Ni Putu Ratna Ayu; Kartika, Titik
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.5829

Abstract

Botanical gardens are areas that provide protection for trees and other organisms like polyporous fungi. Polyporous fungi are important fungi that degrade remaining lignocellulosic in leaf litter or dead trees. These mycobiota are also noted for their vital role in biorefinery, bioremediation, medicine and phytopathogen. The knowledge of the importance of the polyporous fungi to describe polyporous fungal species is fundamental for generating data base information of their occurrence and their functions. This research’s goal was to explore and characterize the polyporous fungi collected in Batam Botanical Garden in three sampling areas. Fungal samples were collected in May and July 2017. Subsequently, morphological characters were recorded, the fungal tissue was isolated to extract the DNA, then the data sequence was amplified and aligned to construct a phylogenetic tree. Five fungal families found belong to order Polyporales and were classified morphologically. They were Polyporaceae, Ganodermataceae, Fomitopsidaceae, Irpicaceae and Hymenochaetaceae. Three fungal species namely; Pycnoporus sanguineus, Trametes ijubarskii, and Antrodia wangii were identified based on phyllogenetic analysis whereas seven other fungal samples were identified as Earliella scabrosa, Hexagonia tenuis, Polyporus tenuiculus Lenzites betulina, Lentinus concavus, Phellinus rimosus and Hexagonia apiaria. This study classifies and adds fundamental databases on fungal taxonomy and diversity on the fungal organisms found in Batam Botanical Garden. This background data is vital to carry out an advance research in some areas such as bio-chemistry, bio-degradation, pharmacology and biotechnology.
Efficacy Test on Some Entomopathogenic Bacterial Isolates of the Periplaneta americana and Blatella germanica Cockroaches (Orthoptera) at Laboratory Scale Zulfiana, Deni; Rini, Monaliza Sekar; Wikantyoso, Bramantyo; Krishanti, Ni Putu Ratna Ayu
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.12934

Abstract

Cockroach is one of insects which plays a role as a disease vector, causing negative impacts to human health. Controlling cockroach by using excessive insecticide adds residue to the environment and strengthens its resistance toward insecticides. The objective of this research was to implement and evaluate the alternative use of biological control agent, such as entomopathogenic bacteria against two types of cockroach. In this study, three entomopathogenic bacterial isolates derived from dead Spodoptera litura pupa and Bacillus thuringiensis were employed. The test was performed by administering spraying and baiting methods at cell concentration of 108 CFU/ml. The results showed that three entomopathogenic bacterial isolates used in this study affected cockroach mortality. The result of morphological observation and Postulate Koch test showed that cockroach mortality was caused by the bacterial isolates. The spraying method on BLSP4 bacterial isolate contributed to the highest mortality rate by 80% to the Blatella germanica. Meanwhile, in the baiting method, the isolate of B. thuringiensis caused the highest mortality to the Periplaneta americana by 10%. Based on the mortality period, it can be recognized that BLSP4 treatment with spraying method affected the mortality of B. germanica effectively within 2 hours 30 minutes 46 seconds. An important finding in this study was that BLSP4 bacteria has been known potential for controlling cockroach using spraying method. The result of this study provides insights that BLSP4 bacteria can be used as a new alternative for controlling cockroach and generally in pest management.