Yuswanto, Ag
*Fakultas Farmasi Universitas Sanata Dharma Jl. Mrican,Yogyakarta 55002

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AKTIVITAS DIURETIK SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM (SNEDDS) FUROSEMID wahyuningsih, iis; sugiyanto, sugiyanto; Yuswanto, Ag; martien, ronny
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol 4 No 1 (2017)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2001.986 KB) | DOI: 10.22236/farmasains.v4i1.3360

Abstract

Furosemide is a diuretic having low solubility and low bioavailability thus the diuretic activity is not optimal. The purpose of this study was to determine the effect of furosemide SNEDDS formation to  the diuretic activity. SNEDDS was made with a mixture of 66% tween 80, 26% propilene glycol, 8% oleic acid and furosemide 40 mg/mL. A total of 20 test animals were divided into 4 groups, namely the negative control 1 (CMC 0,5% suspension), negativ control 2 (a mixture of tween 80, propilene glycol and oleic acid), furosemid suspension and SNEDDS furosemid. Tests on the diuretic activity is done by measuring the volume of urine released for 6 hours.Furosemide SNEDDS  could enhance the diuretic activity of furosemide.
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy Ismail, Hilda; Ciptasari, Ummi H.; Ikhsan, M Arief Nur; Suryani, Fidya; Sismindari, Sismindari; Martien, Ronny; Yuswanto, Ag
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (988.21 KB) | DOI: 10.14499/indonesianjpharm30iss1pp52

Abstract

Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).