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PENGARUH WAKTU PENGAMBILAN SAMPEL DARAH TERHADAP PEMERIKSAAN TESTOSTERON Ardana, Mirhansyah
Journal of Tropical Pharmacy and Chemistry Vol 3 No 1 (2015): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.139 KB) | DOI: 10.25026/jtpc.v3i1.84

Abstract

The research about “The Influence of Sampling Time to Testosterone for Medical Check Up” had been develoved to 19 man aged 19 – 25 years old. Sample was collected at 8 a.m. and 12 a.m. to determine testosterone serum level. Determining of testosteron serum level was done using Electrochemiluminescence Immunoassay(ECLIA). The result show that testosterone serum level mean at 8 a.m. (608.90 ng/dL) is slightly lower than testosterone serum level at 12 a.m. (647.95 ng/dL). The analysis of t test (α=0.05) show that there was not significantly difference between testosteron level mean at 8 a.m. and 12 a.m. So the sampling time to determine testosteron serum level to men can be done until 12.00 a.m.   Keywords: Testosterone, Electrochemiluminescence, Immunoassay   ABSTRAK Telah dilakukan penelitian mengenai “Pengaruh Waktu Pengambilan Sampel Darah Terhadap Pemeriksaan Testosteron”. Sampel darah diambil dari 19 pria berumur 19 – 25 tahun. Sampel darah dikumpulkan pada pukul 08.00 dan pukul 12.00 untuk pemeriksaan kadar testosteron. Penentuan kadar testosteron dilakukan menggunakan metode Electrochemiluminescence Immunoassay (ECLIA). Hasil penelitian menunjukan bahwa kadar testosteron rata-rata pada pukul 08.00 (608,90 ng/dL) sedikit lebih rendahdaripada kadar testosteron rata-rata pada pukul 12.00 (647,95 ng/dL). Hasil analisis uji t (α = 0,05) menunjukan bahwa tidak terdapat perbedaan yang signifikan antara kadar testosteron rata-rata pada pengambilan pukul 08.00 dan pukul 12.00. Sehingga pengambilan sampel darah untuk pemeriksaan kadar testosteron pada pria dewasa dapat dilakukan hingga pukul 12.00.   Kata kunci: Testosteron, Electrochemiluminescence Immunoassay
FORMULASI DAN OPTIMASI BASIS GEL HPMC (HIDROXY PROPYL METHYL CELLULOSE) DENGAN BERBAGAI VARIASI KONSENTRASI Ardana, Mirhansyah; Aeyni, Vebry; Ibrahim, Arsyik
Journal of Tropical Pharmacy and Chemistry Vol 3 No 2 (2015): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.804 KB) | DOI: 10.25026/jtpc.v3i2.95

Abstract

Ideal gel formulation can be obtained by formulating some kind of gelling material, but the most important thing to note is the selection of a gelling agent. HPMC (Hidroxy Propyl Methyl Cellulose) is a gelling agent that is commonly used in the production of cosmetics and drugs, because it can producea gel that is clear, easily soluble in water, and has a low toxicity. This study aimed to obtain the concentration of HPMC as a gelling agent that has the physical stability in accordance with the stipulated requirements. Gel formulations made with HPMC concentration variation of 3%, 5% and 7%, further evaluation of physical properties include organoleptic test, homogeneity, dispersive power, pH, and viscosity. Evaluation gel base done for 3 weeks. The results of stability tests show the base gel with a concentration of 7% HPMC has a good standard for viscosity, pH, dispersive power, homogeneity and organoleptic.Key words: Gel, HPMC (hidroxy propyl methyl cellulose), gelling agent.ABSTRAKSediaan gel yang baik dapat diperoleh dengan cara memformulasikan beberapa jenis bahan pembentuk gel, namun yang paling penting untuk diperhatikan adalah pemilihan gelling agent. HPMC (Hidroxy Propyl Methyl Cellulose) merupakan gelling agent yang sering digunakan dalam produksi kosmetik dan obat, karena dapat menghasilkan gel yang bening, mudah larut dalam air, dan mempunyai ketoksikan yang rendah. Penelitian ini bertujuan untuk memperoleh konsentrasi HPMC sebagai gelling agent yang memiliki kestabilan fisika yang sesuai dengan persyaratan yang telah ditetapkan. Formulasi gel dibuat dengan variasi konsentrasi HPMC 3%, 5% dan 7%, selanjutnya dilakukan evaluasi sifat fisika yang meliputiuji organoleptis, homogenitas, daya sebar, pH, dan viskositas. Evaluasi basis gel dilakukan selama 3 minggu. Hasil yang diperoleh dari uji stabilitas menunjukan basis gel dengan konsentrasi HPMC 7% memiliki standar yang baik untuk viskositas, pH, daya sebar, homogenitas dan organoleptis.Kata Kunci : Gel, HPMC (Hidroxy Propyl Methyl Cellulose), gelling agent.
Synthesis and Bioactivity of 3-(2,4-Dichlorophenyl)-5-(4-Hydroxy-3-Methoxyphenyl) Pyrazoline Khotimah, Nurul; Rahmadani, Agung; Rahmawati, Dewi; Ardana, Mirhansyah
Journal of Tropical Pharmacy and Chemistry Vol 4 No 4 (2018): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.433 KB) | DOI: 10.25026/jtpc.v4i4.183

Abstract

Pyrazoline is a five-ring heterocyclic compound having 3 carbon atoms and 2 nitrogen atoms known to have some biological activity. This study aims to synthesize 3-(2,4-dichlorophenyl)-5-(4-hydroxy-3-methoxyphenyl) pyrazoline using 2’,4’-dichloro-4-hydroxy-3-methoxy chalcone and hydrazine. Synthesis was performed by reflux method at 80oC for 7 hours. The synthesis compounds were characterized by structural elucidation techniques. From the synthesis results obtained compound 3-(2,4-dichlorophenyl)-5-(4-hydroxy-3-methoxyphenyl) Pyrazoline is pale yellow powder with 84%yield. The result of antioxidant activity test by 2,2-diphenyl-1-picrylhydrazyl (DPPH) showed that the compound had very strong antioxidant activity, Antibacterial test by diffusion method to use paper disk showed that the compound had antibacterial activity against Gram positive bacteria (S.aureus) and Gram negative bacteria (E.coli) and toxicity test by Brine Shrimp Lethality Test (BSLT) showed that the compound had toxicity.