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RISET SITOTOKSIK CAMPURAN EKSTRAK DAUN SIRSAK (Annona muricata L) DAN KULIT BUAH MANGGIS (Garcinia mangostana L) PADA SEL VERO DAN AML12 Mardja, Tuty Erlina; Rahmi, Fitria; Rusmawati, Eka; Adriany, Rina; Murtiningsih, Murtiningsih; Setijanti, Herlina B.; Usia, Tepy
Journal of Tropical Pharmacy and Chemistry Vol 3 No 4 (2016): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.93 KB) | DOI: 10.25026/jtpc.v3i4.116

Abstract

Campuran Daun sirsak dan Kulit buah manggis sekarang ini banyak digunakan sebagai fito-terapi penyakit kanker dan antioksidan. Tetapi penggunaan bahan ini belum diketahui data keamanannya. Tujuan dilakukan penelitian adalah untuk mengetahui efek sitotoksik campuran ekstrak daun sirsak dan kulit buah manggis terhadap sel vero dan sel AML12. Uji sitotoksik dapat memprediksi keberadaan senyawa yang bersifat toksik secara in vitro menggunakan sel normal atau sel yang telah mengalami transformasi. Uji ini menggunakan 2 jenis cell line, yaitu sel vero dan sel AML12. Sampel berupa simplisia daun sirsak dan kulit buah manggis yang dibuat ekstrak dengan menggunakan etanol 96%. Konsentrasi uji yang digunakan adalah 100; 50; 25; 12,5; 6,25 dan 3,125 mg/mL untuk sel vero dan 500; 250; 125; 62,5; 31,25 dan 15,625 mg/mL untuk sel AML12. Kultur sel dilakukan di wellplate 96 diinkubasi didalam inkubator CO2 pada suhu 37°C selama 24 jam kemudian ditambahkan sampel uji dan selanjutnya diinkubasi kembali didalam inkubator CO2 pada suhu 37°C selama 24 jam. Langkah selanjutnya uji MTT dan kemudian dibaca dengan ELISA reader pada panjang gelombang 570nm. Diperoleh hasil sebagai berikut: IC50 55,97 mg/mL pada sel vero, dan 43,292 mg/mL pada sel AML12. Kesimpulannya sampel campuran ekstrak daun sirsak dan kulit buah manggis ini mempunyai efek toksik terhadap sel vero dan sel AML12 (IC50<100 mg/mL). Kata kunci: Sitotoksik, Daun sirsak, Kulit buah manggis, sel vero, sel AML12, IC50 Abstract Mix of soursop leaves and mangosteen pericarp use as cancer phytotherapy and antioxidant, but it is not yet known its toxicities data. The aim of this study was evaluate cytotoxicity effect of mix of soursop leaves and mangosteen pericarps extract on vero cells and AML12 cells. Toxicity study is one ways to predict the presence of toxic compounds using normal cells or cells that have undergone a transformation. That study was using vero cell and AML12. Samples was ethanolic extract of soursop leaves and mangosteen pericarp. The test dose were 100; 50; 25; 12,5; 6,25 dan 3,125 mg/mL in vero cell and 500; 250; 125; 62,5; 31,25 dan 15,625 mg/mL in AML12. Cells were cultured in well plate 96 and incubated in CO2, temperature 37°C for 24 hours and then added samples and incubated incubated in CO2, temperature 37°C for 24 hours. Cell was added MTT and read with ELISA reader. The results showed IC50 55,97mg/mL in vero cells and 43,292 mg/mL in AML12. Conclusion of this study is ethanolic extract of soursop leaves and mangosteen pericarps has toxicity effect in vero cells and AML12 cells (IC50<100 mg/mL). Keywords: cytotoxiciy, soursop leaves, mangosteen pericarp, vero cells, AML12 cells, IC50
Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.) MANGUNWARDOYO, WIBOWO; CAHYANINGSIH, ENI; USIA, TEPY
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 2 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1815.081 KB)

Abstract

Phyllanthus niruri L. herbs was extracted using ethanol 96%, ethylacetate and n-hexane. The rendements were respectively 49.25% (for ethanol extract), 4.18% (ethylacetate extract) and0.78% (n-hexane extract). Identification of the ethanol extract using phytochemical assays resulted in alkaloid, flavonoid, saponin, and tannin compounds. Purification of the ethanol extract using thin layer chromatography (TLC) with n-hexane-ethylacetate (6:4) followed by the inhibitory activity test using bioautography TLC showed that the spot of Rf 0,46 inhibited Staphylococcus aureus and that of Rf 0,66 inhibited Candida albicans. Analysis with infra red spectrophotometer showed that the ethanol extract has hydroxyl (-OH) and carbonyl (C=O) functional groups.
Fingerprint Study of Curcuma xanthorrhiza Rhizome by High Performance Thin Layer Chromatography (HPTLC) Wikara, Tina; Sulistiowaty, Anny; Murhandini, Sri; Usia, Tepy
Jurnal Jamu Indonesia Vol 1 No 2 (2016): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1165.946 KB) | DOI: 10.29244/jji.v1i2.13

Abstract

The rhizome of Curcuma xanthorrhiza Roxb is intensively used in Indonesia as traditional medicine. It is widely used for hepatoprotective and anti inflammatory activities. To ensure the quality of its extract, we have studied the fingerprint or phytochemical analysis. This research was aimed to produce a chromatogram profile of the rhizome by HPTLC. The HPTLC fingerprint chromatogram of C. xanthorrhiza rhizome was performed using HPTLC plate of silica gel 60 F254 as the stationary phase and chloroform-methanol (97:3) as the mobile phase. Spot detection was carried out by TLC photo documentary system at 254 and 366 nm and TLC scanner at 427 nm. The developed method was validated according to ICH guidelines by determination of specificity and precision. We found that the specifity and precission of the method were met the acceptance criteria. In conclusion, the developed method is valid and could be used for quality control and standardization of herbal medicine containing C. xanthorrhiza rhizome.