Kartika, Titik
Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Phylogenetic Analysis of Polyporous Fungi Collected from Batam Botanical Garden, Riau Province, Indonesia Lestari, Anis Sri; Zulfiana, Deni; Zulfitri, Apriwi; Krishanti, Ni Putu Ratna Ayu; Kartika, Titik
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.5829

Abstract

Botanical gardens are areas that provide protection for trees and other organisms like polyporous fungi. Polyporous fungi are important fungi that degrade remaining lignocellulosic in leaf litter or dead trees. These mycobiota are also noted for their vital role in biorefinery, bioremediation, medicine and phytopathogen. The knowledge of the importance of the polyporous fungi to describe polyporous fungal species is fundamental for generating data base information of their occurrence and their functions. This research’s goal was to explore and characterize the polyporous fungi collected in Batam Botanical Garden in three sampling areas. Fungal samples were collected in May and July 2017. Subsequently, morphological characters were recorded, the fungal tissue was isolated to extract the DNA, then the data sequence was amplified and aligned to construct a phylogenetic tree. Five fungal families found belong to order Polyporales and were classified morphologically. They were Polyporaceae, Ganodermataceae, Fomitopsidaceae, Irpicaceae and Hymenochaetaceae. Three fungal species namely; Pycnoporus sanguineus, Trametes ijubarskii, and Antrodia wangii were identified based on phyllogenetic analysis whereas seven other fungal samples were identified as Earliella scabrosa, Hexagonia tenuis, Polyporus tenuiculus Lenzites betulina, Lentinus concavus, Phellinus rimosus and Hexagonia apiaria. This study classifies and adds fundamental databases on fungal taxonomy and diversity on the fungal organisms found in Batam Botanical Garden. This background data is vital to carry out an advance research in some areas such as bio-chemistry, bio-degradation, pharmacology and biotechnology.
Pengembangan Formula Bahan Infeksi Cendawan sebagai Alternatif Biokontrol Rayap Tanah Coptotermes sp. Development of Infection Material Formula for Fungi as Bio-Control Alternative to Subterranean Termites Coptotermes sp Kartika, Titik; Yusuf, Sulaeman; Tarmadi, Didi; Prasetyo, Arief Heru; Guswenrivo, Ikhsan
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 5, No 2 (2007): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.875 KB)

Abstract

Utilization of fungal entomopathogen as biological control agent has been developed in several country, but not in Indonesia. Therefore, the use of biological control agent to control termite need to be done in order that diminish chemical insecticide hazard. In this research, the ability of fungal entomopathogen (Hyphomycetes) identified as Metarhizium anisopliae, Beauveria sp. and Humicola sp. to infect Subterranean termite will be evaluated. The fungi were isolated from Indonesia. The research methods are (i) to sporulate fungi in rice culture media; (ii) to formulate fungal entomopathogen being infection materials; (iii) bioassay of fungal entomopathogen against Subterranean termite by contact method. The result of bioassay show that the three fungi (Humicola, M. anisopliae and Beauveria) are able to kill termite in 14 days of observation day. Primarily, M. anisopliae has generate termite’s mortality almost similar to Humicola sp. i.e higher than 60 %, in other side Beauveria just affect termite’s mortality lower than 60 %. 
Daya Patogenitas Cendawan Cunninghamella sp. terhadap Rayap Tanah Coptotermes sp. Pathogenic Ability of Cunninghamella sp. against Subterranean Termites Coptotermes sp Kartika, Titik; Suciatmih, Suciatmih; Tarmadi, Didi; Guswenrivo, Ikhsan; Prianto, Arief Heru; Yusuf, Sulaeman
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 4, No 1 (2006): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.355 KB)

Abstract

Termites are the major agent in wood biodeterioration, particularly in building materials, thus its existence in wood become trouble. Chemical insecticides are widely used to prevent the lost from termites attack. The use of chemical insecticides, however, do not recomended as it may cause risk to human health, environment, and may also harm to the non-target organisms and lead to the development of pest resistance. For that reason, these situations encourage many scientists to develop and evaluate various prospective biological control of termites. One of them is utilization of entomopathogenic fungi to suppress termite population.The object of this research is learning pathogenic ability of Cunninghamella sp. (habitually found around termites nests) in termite’s mortality. The methods of this research are fungal isolation and then pathogenic test to termites (termite’s infection). The ways of termite infection are contact and digestion. The best method is based on termite’s mortality rate after infection.The results show that Cunninghamella sp. causes termites mortality significantly than control. The higher mortality rate of termites is shown by contact infection method than digestion infection method. It might indicate that contact infection method more effective to suppress termite’s population
Pemanfaatan Ekstrak Enzim Jamur Humicola sp. Sebagai Biotermitisida Utilization of Humicola sp. Enzyme Extract as Biotermiticide Guswenrivo, Ikhsan; Kartika, Titik; Tarmadi, Didi; Yusuf, Sulaeman
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 6, No 1 (2008): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.91 KB)

Abstract

Fungal spore can be used for biotermiticide, since it has high pathogen properties and environmentally safe. However, the utilization of enzyme extract of fungi can be developed as an alternative way to minimize the impact caused by the spore when they are used as biotermiticide. This research was done to observe the ability of enzyme extract from Humicola sp. The fungus was inoculated in sorghum substrate and grown in it by adding either colloidal chitin or without colloidal chitin to the media as variation of the medium composition. Extraction was done after 3, 5, and 7 days of incubation time. Extract was done by using cold sterilized water (40C). Then the extract kept it at low temperature (40C). Bioassay of extract enzyme was done by spraying and baiting method. Result of the bioassay showed, Humicola sp enzyme extract was grown from medium adding with colloidal chitin and incubation for 7 days give highest mortality of termite increase to 100% after 14th day observation. 
Pengaruh Ekstrak Bintaro (Carbera odollam Gaertn) dan Kecubung (Brugmansia candida Pers) terhadap Rayap Tanah Coptotermes sp Influence of Bintaro (Carbera odollam Gaertn) and Kecubung (Brugmansia candida Pers) Extract against Subterranean Termite Coptotermes sp Tarmadi, Didi; Prianto, Arief H; Guswenrivo, Ikhsan; Kartika, Titik; Yusuf, Sulaeman
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 5, No 1 (2007): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.259 KB)

Abstract

Subterranean termite especially Coptotermes sp has been spread widely and has caused a tremendous economical loss. Various chemical compounds have been used to overcome termite attack. However, utilization of chemical could endanger the environment, thus it is important to find another compound which can be used as an environmental friendly wood preservatives. One of the alternatives is to extract natural compound which has an anti-insect peculiarity. Fruits, leaves and barks of Bintaro and Kecubung, especially their leaves and flowers have been widely known as traditional medicine. This paper explains the effects of the extracts of Bintaro (leaves and bark) and Kecubung (leaves) on subterranean termite of Coptotermes sp.Leaves and bark powder of Bintaro and leaves powder of Kecubung were extracted with n-hexane, ethyl acetate, acetone, and methanol. Paper disc which has been dropped by the extract solution was used as bait to subterranean termite of Coptotermes sp. The observation of termite mortality was calculated for every two days during 10 days of observation. The result shows that Bintaro leaves extracted with methanol caused 100% termite mortality on last observation. Whereas on Bintaro bark extracted with n-hexane and acetone caused 100% termite mortality on eighth day of the treatment. Kecubung leaves extracted with n-hexane and ethyl acetate caused 100% termite mortality on last observation