Elisabeth, Mariska
The Prodia Education and Research Institute (PERI)

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Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin Rusmana, Djaja; Wahyudianingsih, Roro; Elisabeth, Mariska; Balqis, Balqis; Maesaroh, Maesaroh; Widowati, Wahyu
The Indonesian Biomedical Journal Vol 9, No 2 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i2.281

Abstract

BACKGROUND: Normal metabolism of oxygen and exogenous factors constantly generate free radicals which could be harmful to the human body. Human need antioxidants to provide protection against free radicals, thus plants are a good source of natural antioxidants. Phyllanthus niruri (P. niruri) has been known to possess several medicinal properties and contain numerous active phytochemical. In this research, we conducted phytochemical screening and antioxidant assay of P. niruri extract along with the compounds rutin and quercetin, which are flavonoids possessing medicinal properties. This study was conducted to determine P. niruri, rutin and quercetin as antioxidant.METHODS: In this study, qualitative phytochemical screening was performed to detect phenol, flavonoid, saponin, tannin, steroid/triterpenoid, terpenoid and alkaloid in P. niruri extract. Antioxidant analysis of P. niruri, rutin and quercetin was conducted using total measured phenolic content, 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2’-azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays.RESULTS:  The study revealed that P. niruri extract contained saponin, phenol, flavonoid and tannin based on phytochemical screening. In DPPH and ABTS assays quercetin possessed highest antioxidant activity with IC50 value of 0.55 and 1.17 μg/ml respectively. Meanwhile, P. niruri extract showed the highest FRAP activity which was 373.95 μM Fe(II)/μg extract. Rutin possessed the lowest antioxidant activity in all antioxidant assays.CONCLUSION: This study confirmed that P. niruri extract and quercetin have great potential as a natural antioxidant source.KEYWORDS: asntioxidant, phytochemical, Phyllanthus niruri, quercetin, rutin, free radical
The Influence of Obesity on Blood Pressure in Early Adulthood and Middle Adulthood Males Eusman, Muhammad O A; Ratnawati, Hana; Elisabeth, Mariska
Journal of Medicine and Health Vol 2 No 3 (2019)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.573 KB) | DOI: 10.28932/jmh.v2i3.1222

Abstract

Hypertension is commonly found in obesity patients. Age affects the occurrence of hypertension. The aim of this study is to determine the effect of obesity on blood pressure levels in early adulthood males (<35 years) and middle adulthood males (?35 years). This is an observational analytic study using data from medical check-up records of male adult office workers in Bandung. The data was analyzed with Mann-Whitney test (? = 0.05) and Chi-Square to find the odds ratio (OR). The results showed that out of 110 data in medical records, there were 79 (71.82%) obese and 31 (28.18%) non-obese. In early adulthood males (<35 years) there is no significant difference between non-obese with obese in systolic blood pressure (p=0.779) and diastolic blood pressure (p=0.779). In middle adulthood males (?35 years) there was a highly significant difference between non-obese with obese on systolic blood pressure (p=0.001) and diastolic blood pressure (p=0.003). There was a significant relationship between obesity and hypertension (p=0.048) with OR=3.73. In conclusion, in early adulthood males (<35 years) obese does not have an effect on the development of hypertension, while in middle adulthood males, obesity increases the risk of developing hypertension by 3.73 times. Keywords: obesity, blood pressure, early adulthood males, middle adulthood males