Jurnal Perikanan Universitas Gadjah Mada
Vol 11, No 1 (2009)

ISOLASI, KARAKTERISASI DAN UJI INFEKSI BAKTERI PROTEOLITIK DARI LUMPUR KAWASAN HUTAN BAKAU

Triyanto, Triyanto (Unknown)
Isnansetyo, Alim (Unknown)
Prijambada, Irfan D. (Unknown)
Widada, Jaka (Unknown)
Tarmiawati, Afi (Unknown)



Article Info

Publish Date
20 Feb 2009

Abstract

This research was aimed to isolate proteolytic bacteria that have high ability to hydrolize protein. The sources of bacterial samples were collected from mangrove sediment in Indramayu and Cilacap Regency. Collwel SWYE Modification Agar medium and Skim Milk Agar Medium, which contain pepton and casein, were used for isolating the bacteria. Forty isolates having proteolitic activity were obtained. Three isolates, i.e. Ps.23.1, Ps.27.3 and Ps.23.2, having the highest activity were selected. The selected isolates were able to grow at pH range of 5 to 8. They were also able to grow at NaCl concentration of 0.5-3.5%. Intramuscular injection test on white shrimp (Lithopenaeus vannamei) shows that Ps.23.1 and Ps.23.2 were not pathogenic to shrimp. On the other hand, Ps.27.3 was indicated to cause disease on shrimp. These results indicated that isolates Ps.23.1 and Ps.23.2 potentially can be used as inoculant for bioremedation of brackish water pond. Isolate Ps.23.1 was characterized to be an aerobic, nonmotile, short rod, gram negative bacteria having catalase and oxidase activities. It produced yellowish, circulair, entire colonies. Isolate Ps.23.2 was characterized to be an aerobic, motile, rod, spore forming, gram positive bacteria having catalase and oxidase activities.

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Journal Info

Abbrev

jfs

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider ...