The objective of the research was studied the effect ofÂ acetic acid concentration and extraction time on the collagen quality of black tilapia leather. Black tilapia leather processed into collagenÂ as an alternative to increasing value-added of fisheries industry waste. Collagen of black tilapia was extracted by the treatment of acetic acid molarity, each: 0.25 M, 0.50 M, and 0.75 M (A factor) and Â extraction time of 16 and 48 hours (B factor). Based on the analysis of variance, is known that the interaction of those treatments (AB) didnât significantly effect on the yieldÂ (p>0.05). Collagen extraction of tilapia leather with 0.75 M of acetic acid at 16 hours, produces the greatest yieldÂ (5.97%), with denaturation temperature is 35.75oC, and quantitative composition of glisine, alanine, and glutamic amino acids were: 5395.82 ppm (52.99%), 2979.15 ppm (22.08%), and 1684.42 ppm (7.45%). Based on the analysis of SDS-PAGE, is known that the collagen contained were Î± component and Î² component, so that collagen of tilapia leather has type I collagen.
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