Jurnal Ilmu Ternak dan Veteriner
Vol 1, No 3 (1995)

Evaluation of antibody responses of cattle and buffaloes to Clostridium perfringens type A vaccine using ELISA

Natalia, Lily (Unknown)

Article Info

Publish Date
25 Feb 2014


An enzyme-linked immunosorbent assay (ELISA) was developed to detect specific antitoxin Clostridium perfringens type A. It was based on the use of purified alpha toxin of Clostridium perfringens type A as the coating antigen, which was then linked to specific alpha antitoxin . Horse raddish peroxidase labelled IgG was used as the conjugate, and 2,2-azino-bis (3- ethylbenzthiazoline-6-sulfonic acid) (ABTS) as the substrate . The ELISA was used to evaluate vaccination results on cattle and buffaloes against enterotoxaemia caused by Clostridium perfringens type A. Key words: ELISA, Clostridium perfringens type A antitoxin, vaccination

Copyrights © 1995

Journal Info







Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic ...