Indonesian Journal of Biotechnology
Vol 22, No 1 (2017)

Bioactivity and genetic screening of marine actinobacteria associated with red algae Gelidiella acerosa

Ulfah, Maria (Unknown)
Kasanah, Noer (Unknown)
Handayani, Niken Satiti Nur (Unknown)

Article Info

Publish Date
01 Jun 2017


Bacterial resistance to existing antibiotics has driven a search for new antibiotics from marine actinobacteria. Bioactivity and genetic screening of actinobacteria associated with red algae Gelidiella acerosa were conducted to discover new antibacterial compounds against Vibrio alginolyticus. A total of 14 actinobacteria isolates were obtained from G. acerosa. The isolates were subjected to genetic screening for nrps (non-ribosomal peptide synthetase) and FADH2-dependent halogenase genes. The isolates’ ability to produce secondary metabolites was examined by fermentation in various media in a six-well mini plate. The bioactivity of the secondary metabolites was screened using a microtiter assay and the agar overlay method. The results showed that all 14 isolates had the nrps gene, whereas none had the halogenase gene. Meanwhile, eight of the actinobacteria isolates showed antibacterial activity against V. alginolyticus.

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Journal Info





Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology


The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...