Vibriosis is one of diseases which often results in mass mortality ofÂ Penaeus monodonÂ larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarvaÂ stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescentÂ bacterium). Several kinds of antibiotics and chemical material have been used toÂ overcome the disease but they have side effects to environment and human. TheÂ searching of bioactive compounds as an alternative treatment has been done forÂ multi purposes. In this study diethyl eter, butanol and aqueous extract of IndonesianÂ sponges Aaptos aaptos and Callyspongia pseudoreticulataÂ were tested for in vitroÂ activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. TheÂ result showed that all extracts of Aaptos aaptos gave a positive antibacterial activityÂ towards those pathogenic bacteria. Meanwhile, only butanol extract of CallyspongiaÂ pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenicÂ bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extractÂ of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 andÂ 0.625 mg/mL, respectively. Whilst, the butanol extract ofÂ Callyspongia pseudoreticulataÂ indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity ofÂ those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly,Â butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect inÂ Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the firstÂ report for the anti-vibr io activity of both Aaptos aaptos and CallyspongiaÂ pseudoreticulata. This results suggest thatÂ Aaptos aaptos has a potential to be usedÂ as a source of alternative compound to vibriosis prevention for mariculture.
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