Journal of Tropical Crop Science
Vol 2 No 2 (2015): Journal of Tropical Crop Science

Study of Oil Palm (Elaeis guineensis Jacq) In Vitro Embryogenesis using Young Leaves Explants

Wiendi, Ni Made Armini (Unknown)
Constantin, Mondjeli (Unknown)
Wachyar, Ade (Unknown)



Article Info

Publish Date
01 Jun 2015

Abstract

This study reported in vitro embryogenesis of oil palm using young leaves as explants. Explants were grown in solid modified MS or Eeuwens medium containing different concentrations of NAA and 2,4-D, i.e. media C1, C2, C3, C4 and C5, M1, M2, M3 and M4, to induce embryogenic calli. Compact and pearly-white, globular calli were obtained from the youngest leaf explants 28 weeks after culture.C1 media (MS medium + 107.41 µM of NAA + 100 mg.L-1 of asparagine + 100 mg.L of glutamine-1) produced the highest percentage of calli formation (30.56%), whereas C4 media (C1 supplemented with 67.86 µM of 2,-D ) was the optimal media for embryogenic callus induction. Direct embryoids were obtained from slightly older leaf explants on the C3 media containing NAA after 36 weeks of culture. However, four subcultures using the same medium with gradual reduction of auxin concentration were not successful to develop embryogenic callus and embryoid cells during the course of this study.  

Copyrights © 2015






Journal Info

Abbrev

agro

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Journal of Tropical Crop Science is an international journal publishing research related to tropical crops. Articles in the journal focus on in-vivo and in-vitro production and production environment, breeding, seed technology, physiology, postharvest, pest and pathology of cereals, vegetables, ...