Indonesian Journal of Cancer Chemoprevention
Vol 8, No 2 (2017)

Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Lestari, Beni (Unknown)
Novitasari, Dhania (Unknown)
Putri, Herwandhani (Unknown)
Haryanti, Sari (Unknown)
Sasmito, Ediati (Unknown)
Meiyanto, Edy (Unknown)

Article Info

Publish Date
30 Jun 2017


Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words : food additives, genotoxic, cytotoxic, micronucleus

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Journal Info





Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology


Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, ...