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Medical Laboratory Technology Journal
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Core Subject : Health, Science,
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area. Please read these guidelines carefully. Authors who want to submit their manuscript to the editorial office of Medical Laboratory Technology Journal should obey the writing guidelines. If the manuscript submitted is not appropriate with the guidelines or written in a different format, it will BE REJECTED by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.
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Articles 83 Documents
THE CYTOTOXIC ACTIVITY OF ANNONA MURICATA LINN LEAVES ETHANOLIC EXTRACT (AMEE) ON T47D BREAST CANCER CELL LINE Fertilita, Soilia; Sandhika, Willy; Suprabawati, Desak Gede Agung
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.291

Abstract

Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.
THE RESULTS OF TOXOPLASMA GONDII EXAMINATION IGM ANTIBODIES USING ICT METHOD AND ELISA METHOD IN PREGNANT WOMEN Nadayang, Syifa Amelia; Fajrunni'mah, Rizana
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.241

Abstract

Toxoplasmosis is a disease that can infect animals and zoonotic in humans. In pregnant women (congenital toxoplasmosis), the risk and severity of symptoms of congenital toxoplasmosis are more severe if infection occurs early in pregnancy and asymptomatic. The only way to determine disease is by serological screening. Serological diagnosis techniques for toxoplasmosis include ELISA (Enzyme-linked Immunosorbent Assay) and ICT (Immunochromatography Assay Test). However, until now, research on toxoplasmosis with the ICT method is still minimal. This study aims to analyze the results of the IgM antibody examination Toxoplasma gondii using the ICT method with the ELISA method and calculate the sensitivity and specificity of the ICT method and the ELISA method in pregnant women. The research design was used analytic (cross-sectional), with the number of samples is 33 serums. Data from the ELISA method obtained from secondary medical record data and the remaining serum samples were examined by the ICT method by researchers at the Harapan Kita Hospital Laboratory. In the ICT method, two samples (6.1%) were positive, and 31 samples (93.9%) were negative. In the ELISA method, results obtained similar to the ICT method, two samples (6.1%) positive and 31 samples (93.9%) with negative results. Toxoplasmosis examination can be done using the ICT method as the initial serological screening stage, but it not recommended as a diagnosis enforcer.
ANIMAL MODELS WITH METABOLIC SYNDROME MARKERS INDUCED BY HIGH FAT DIET AND FRUCTOSE Hidayati, Larantika; Widodo, Agung Dwi Wahyu; Hidayat, Boerhan
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.266

Abstract

Metabolic syndrome is lipid and non-lipid metabolism disorder due to the association of several factors such as physiological, clinical, biochemical, and interrelated factors. People with metabolic syndrome can be diagnosed by fulfilling 3 of 5 criteria, including obesity and increased waist circumference, increased TG levels, increased blood pressure, hyperglycemia, and increased High-Density Lipoprotein (HDL) serum. The high-fat diet disrupts tissue lipid metabolism, so insulin resistance occurs due to lipotoxicity. Besides, some studies use a combination of mixtures (fructose, sucrose) and fat-rich food components to build metabolic characteristics in mice that affect human characteristics. The purpose of this study was to make an animal model with a metabolic syndrome marker induced by the High Fat Diet (HFD) consisting of pork oil and chicken egg yolk, as well as fructose from simple and economical ingredients. This study was an experimental study using experimental animals of male Rattus norvegicus strain wistar, which were grouped into three random treatment groups, namely the control group, HFD group, and High Fat Diet Fructose (HFDF) group. The number of samples used was 27 rats, with nine rats in each group. The animal was induced for four weeks, then measured levels of FPG, HD, and TG. From the research that has been done, it is found that there are significant differences in levels of Fasting Plasma Glucose (FPG), High-Density Lipoprotein (HDL), and Triglycerides (TG) (p
RENAL FUNCTION EVALUATION IN SECONDARY AMYLOIDOSIS ACCORDING TO LOCATION OF DEPOSITION Özçelik, Serhat; ?ahin, Gülizar Manga
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.284

Abstract

It aimed to evaluate the renal functions according to the accumulation site of amyloid in patients diagnosed with secondary amyloidosis by renal biopsy. Fifty patients diagnosed with secondary amyloidosis, who referred to the nephrotic syndrome outpatient clinic included in the study. According to the findings of renal biopsy, only-glomerular amyloid deposition (group- 1) detected in 9 patients and glomerular+ tubulointerstitial deposition (group- 2) identified in 41 patients. Serum creatinine, creatinine clearance, and total proteinuria levels compared between groups at the time of presentation and on 3rd, 6th, 12th, and 24th months. Initial and subsequent serum creatinine, creatinine clearance, and total proteinuria levels not found to be significantly different between groups (p>0,05 for all). Among subjects with only-glomerular deposition, statistically significant descents in total proteinuria, when compared to initial levels, were observed from the beginning of the 6th month to the end of the 24th month (p
THE EFFECT OF DEHYDROEPIANDROSTERONE ON OVARIAN RESERVE IN OVARIAN DAMAGE CAUSED BY METHOTREXATE An??n, Ali Do?ukan; Çö?endez, Ebru; Gün, ?smet; Sakin, Önder; Ç?kman, Muzaffer Seyhan; Ba?ak, Kayhan; Orçun Kaptana?as?, Asuman; Alan, Yasemin; Alan, Murat; Beyan, Emrah
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.270

Abstract

To determine the possible detrimental effects of multiple methotrexate doses has on the ovarian reserve and to determine the beneficial effects of dehydroepiandrosterone supplementation. The rats (n:24) divided into three groups; Group 1: control group, Group 2: dehydroepiandrosterone and methotrexate group (6mg/kg dehydroepiandrosterone dissolved in 0.1 ml sesame seed oil subcutaneously for ten days and 1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th days) and Group 3: methotrexate group (1mg/kg intramuscular methotrexate at the 1st, 3rd, 5th and 7th day). The groups compared in regards to their histopathological ovarian damage scores and AMH values. It established that multiple methotrexate applications had a considerable effect on reducing vascular congestion in the ovarian tissue. Both in groups 2 and 3 AMH values found to be significantly lower. When this decline in the ovarian reserve examined comparatively; while both the group 2 and 3 reported having a considerable and continuous reduction in the AMH levels correlative to the control group; the primordial, primary and total follicle counts shown to stay statistically the same in the group 2 (p
THE EFFECT OF ORAL CONTRACEPTIVE PILLS ON THE GENE MUTATION OF FACTOR V LEIDEN AMONG SUDANESE WOMEN Mohammed Salih, Kawthar Abdelgaleil; Abdelmalik, Hiba; Babiker, Hiba; Abd Alla, Ahmed Bakheet
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.271

Abstract

Oral contraceptive pills are problems for women, often have many effects, and may cause several diseases. The purpose of this research was to determine the impact of oral contraceptive pills on factor V sufferers. This case-control study conducted in Khartoum Sudan during the period from April to November 2018. The study included 50 women who used oral contraceptive as a case and 50 women who did not use oral contraceptive pills as a control, all of whom were verbally informed of the study and approved for participation. The PCR do for each sample. The results obtained from cases show that the mean age is 30±5.5 and divided into three groups less than 20 with a lower frequency of 4 % (2/50), (20-35) with a higher incidence of 80 % (40/50) and a higher rate of 16 % (8/50) for more than 35 years. Most cases use the oral contraceptive pill for more than one year at a frequency of 60% (30/50) with a mean of 2±0.8. The most frequent oral contraceptive pill use was levonorgestrel 88% (44/50), followed by desogestrel 12% (6/50). The study concludes that there is no significant difference in gene mutation between case and control. There was also an insignificant association between the mutation and demographic data.
BENEFICIAL EFFECTS OF SOYGURT INTAKE IN TYPE 2 DIABETES MELLITUS IN ANIMAL MODEL RAT (RATTUS NORVEGITUS) Bintari, Ni Wayan Desi; Parwati, Putu Ayu
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.258

Abstract

Type 2 Diabetes Mellitus (T2DM) is the more common type of diabetes results from the ineffective use of insulin. Improvement of the metabolic system in T2DM patients can be done through the regulation of gut microbiota balance. Gut microbial improvement can be modulated directly by probiotic food consumption. Soygurt is probiotic food with a low glycemic index (GI) and glycemic load (GL) value and rich in isoflavones, which has a potential effect in reducing diabetes risk. The aim of this study is to determine the effect of soygurt consumption in blood glucose levels and body weight of albino wistar rats (Rattus norvegitus). Reseach using a completely randomized design for experimental study. Subjects of this research are 30 male rats (R. norvegistus) aged 2-3 months with average body weight 150-200 gr. Diabetic rats were induced by using single intraperitoneal injection (175 mg/kg BW) alloxan monohydrate. Soygurt feeding given once daily using oral gavage feeding. The result showed that soygurt feeding in diabetic rats with three variations of treatment could significantly (p
A STUDY RELATED TO EFFECTS OF THE BRUCELLOSIS ON OSTEOPOROSIS Sayiner, Hakan Sezgin; Akgün, Sad?k; Küçükkaya, Yunus; Ba?aran, Murat Alper; Vural, Hüseyin
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.269

Abstract

Brucellosis is one of the infectious diseases that may increase osteoporosis risk. Bone formation and destruction markers analyzed in the examination of osteoporosis risk. There been many studies on the effect of a variety of diseases on the bone, but no studies have conducted for brucellosis. This study is one of the rare studies showing the effect of brucellosis on a bone. The purpose of this study is that whether age, agglutination level, having brucellosis at the time or before, and gender knowledge of patients have effects on osteoporosis by using bone formation and destruction markers. Between 01/04/2015 and 31/12/2017, blood samples were taken from 40 patients with brucellosis and seven patients whose treatment completed at least six months before. Then biochemical markers were studied on these blood samples. ELISA washer and reader (Biotek, Novatek, Istanbul, Turkey) was used to obtain the values of bone formation and destruction markers. There was a significant difference, according to H. Osteocalcin (Human Osteocalcin/Bone Gla Protein), in terms of bone formation markers and was higher in women. Having brucellosis at the time was significant according to Human deoxypyridinoline (DPD) and Human C-telopeptide of type I collagen (CTX-I) in terms of bone destruction markers and was higher in brucellosis. The bone formation and destruction markers strongly correlated with each other in the same direction. It is thought that brucellosis can increase bone destruction markers, especially DPD (Human deoxypyridinoline) and CTX-I; therefore, osteoporosis risk in brucella patients can reduce by implementing a treatment plan that closely monitors bone destruction markers.
PENGARUH EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella Sativa) TERHADAP EKSPRESI ET-1 AORTA PADA MENCIT MODEL PREEKLAMPSIA Hipni, Rubiati
Medical Laboratory Technology Journal Vol 2, No 1 (2016): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v2i1.37

Abstract

Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p
Perbandingan Penurunan Kadar Formalin pada Tahu yang Direbus dan Direndam Air Panas muntaha, akhmad; haitami, haitami; hayati, Nurul
Medical Laboratory Technology Journal Vol 1, No 2 (2015): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i2.20

Abstract

Tofu is a food with high protein content and the moisture content reaches 85%, so that it knows can not last long. Manufacturers know still use formaldehyde as a preservative. Handling to reduce formaldehyde levels in the know are soaked in hot water and boiled in boiling water. The purpose of this study was to determine the ratio decreased levels of formaldehyde in the know are boiled and soaked in hot water out. This type of research is True Experiment with posttest study design Only Control Group Design. The population in this study is tofu containing formalin. Then examined by spectrophotometry of formaldehyde levels in most groups as a pretest sample, others are given treatment and formalin levels checked by spectrophotometry. Data were analyzed using the Mann-Whitney test. Results of this study the average levels of formaldehyde in the know before the treatment is 68.668 ppm. Decreased levels of formaldehyde in formalin know after boiling for 10 minutes was 64.77%. Decreased levels of formaldehyde in formalin know with the treatment of immersion in hot water for 10 minutes is 33.1%. Based on statistical tests that have been conducted, it was found a significant difference between the reduced levels of formaldehyde in formalin boiled out and the know formalin soaked in hot water with sig. 0,000. Boiling know formalin reduce levels of formaldehyde greater than soaking out in hot water.