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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
ISSN : 20895690     EISSN : 24069272     DOI : -
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 210 Documents
Potential Risk of Organic Contaminants to The Coastal Population Through Seafood Consumption from Jakarta Bay Dwiyitno, Dwiyitno; Andarwulan, Nuri; Irianto, Hari Eko; Lioe, Hanifah Nuryani; Dsikowitzky, Larissa; Ariyani, Farida; Schwarzbauer, Jan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.289

Abstract

A comprehensive study on exposure assessment of the priority organic contaminants via seafood consumption has been conducted to the coastal population of Jakarta Bay. Seafood is essential food source in Indonesia and also important income for the majority of coastal populations. A number of 152 respondents from 4 districts surounding the bay were interviewed to record their frequency and pattern on seafood consumption. In the same time, 13 seafood species were collected directly from Jakarta Bay during the dry and wet seasons for the assessment of organic contaminants. A non-target GC/MS screening identified more than 40 organic contaminants in which 6 of them are potentially considered as priority contaminants including 3 groups of carcinogenic contaminants i.e. dichlorodiphenyl-trichlorethane (DDT) and its metabolites (DDXs), dichlorobenzenes (DCB) and carcinogenic PAHs (PAH4). Further exposure analysis suggested cumulative health risk of these contaminants was less than official minimal risk level (MRL) and therefore categorized safe for the corresponding population. However, attention must be paid since additional exposure of either from the different food category or other exposure route may contribute to significantly elevate the health risk on the population as well as potential exposure of emerging contaminants.
usage hydraulic press to separate sap from Eucheuma cottonii Basmal, Jamal
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 2 (2011): August 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i2.62

Abstract

Function of hydraulic press in fresh handling of seaweed is to separate sap from its thallus tofasten its drying process. A device model of hydraulic press has been developed at the ResearchCenter for Marine and Fisheries Product Processing and Biotechnology with a pressure energy(force power) of 30 tons at 4 kWh. During the use of force power of 10 tons/900 cm2for 10 minutesfor 10 kg of seaweed, 20.6% of sap was obtained. Sap is known to contain 2,000 ppm of auxin,1,500 ppm of giberelin (GA3), 1,200 ppm of zeatin and 1,000 ppm of kinetine. In horticulture, ithas been applied to promote growth rate in plants and to increase the production of fruits.
Ethanolic Fermentation Efficiency of Seaweed Solid Waste Hydrolysates by Saccharomyces cerevisiae Martosuyono, Pujoyuwono; Munifah, Ifah; Ningrum, Gesty Aulia
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.231

Abstract

The objective of this research was to determine the saccharification and the fermentation efficiency of seaweed solid waste hydrolysate by Saccharomyces cerevisiae (Accession number 3012.254) in anaerobic condition. The optimum saccharification yield of acid pretreated waste (40.93±1.72)% was obtained after 48 hours with  saccharification rate of (0.51±0.02) g/l.h. Higher yield was shown by NaOH pretreated waste (67.29±1.24)% after 24 hours with saccharification rate of (0.81±0.06) g/l.h. The fermentation of enzymatic hydrolysates of acid and alkali pretreated samples by S. cerevisiae produced a maximum of 7.52±0.24 g/l and 14.5±0.54 g/l ethanol respectively after 72 hours fermentation. Maximum ethanol yield was 0.31±0.03 g/g and 0.40±0.02 g/g sugar respectively for acid and alkali pretreated samples. The ethanol yields showed that alkali pretreated sample produces higher conversion substrate ratio (80% of theoretical yield) compared to acid pretreated sample (62% of theoretical yield).
SYNTHESIS OF POLYVINYL ALCOHOL-CHITOSAN HYDROGEL AND STUDY OF ITS SWELLING AND ANTIBACTERIAL PROPERTIES Wikanta, Thamrin; Erizal, Mr; Tjahyono, Mr; Sugiyono, Mr
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i1.10

Abstract

The aim of this research was to synthesize a hydrogel for wound dressing by mixing of polyvinyl alcohol (PVA) and chitosan (CTS) and processed by combination technique of freezing-thawing and irradiation by gamma ray, and to study of its properties. PVA aqueous solution 10% (w/v) was mixed with 2% (w/v) chitosan (CTS) solution and homogenized. The PVA-CTS mixture was processed by freezing-thawing up to 3 cycles, and then irradiated by gamma rays at the doseranged of 20-50 kGy  (dose rate was 10 kGy/hour). Result showed that PVA-CTS hydrogel with the gel fraction of 83%, 87%, 90%, and 83% were obtained at the irradiation dose of 20 kGy, 30 kGy, 40 kGy, and 50 kGy, respectively. Increasing of irradiation dose caused increasing of water absorption of hydrogel, i.e. 1.700 %, 1.715 %, 1.913 %, and 2.036 %, respectively, and the hydrogel reached the equilibrium in 25 hours. The hydrogel showed very slow water evaporation rate (~ 2%) at the initial time (1 hour) and then increased very fast (up ~50 %) at 24 h, i.e. 43%, 39.13%, 44%, and 53%, respectively. The elongation at break of hydrogels were obtained 245%, 322%, 322%, and 205% with the maximum value were obtained at irradiation dose ranged of 30-40 kGy. The presence of chitosan in the PVA hydrogel made it having higher antibacterial properties with the inhibitionzone value of 8 mm at irradiation dose of 30-40 kGy compared to PVA hydrogel as a negative control (6 mm) and to chloramphenicol as a positive control (8 mm).
ZEASANTIN DARI MIKROALGA: BIOSINTESIS DAN PEMANFAATANNYA Abidin, Dispanstiani; Karwur, Ferry
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 3 (2009): December 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i3.154

Abstract

Karotenoid merupakan pigmen alami yang disintesis oleh semua organisme fotosintetik, serta sejumlah bakteri dan jamur non-fotosintetik. Ada dua pengelompokan karotenoid alami: (1) karoten, seperti â-karoten dan á-karoten, dan (2) santofil. Umumnya santofil seperti violasantin, antherasantin, zeasantin, neosantin, dan lutein diproduksi oleh tumbuhan tingkat tinggi, namun juga dapat disintesis oleh mikroalga hijau.  Zeasantin merupakan salah satu pigmen yang berperan penting  dalam  pencegahan  penyakit  degenerasi  makular  akibat  usia (AMD). Produksi zeasantin dapat  dilakukan oleh  beberapa mikroalga seperti Spirulina sp., Dunaliella salina, Chlamydomonas reinhardtii, Synechocystis, Microcystis aeruginosa, dan Porphyridium cruentum. Artikel ini akan membahas mengenai biosintesis zeasantin serta pemanfaatannya.
Front Cover Squalen Bulletin Vol. 10 No. 1 Tahun 2015 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.336

Abstract

The Quality of Alkali Treated Cottonii (ATC) Made from Eucheuma cottonii Collected from Different Regions In Indonesia darmawan, muhamad; Bandol Utomo, Bagus Sediadi; Yuda Mulia, Raekal Amral
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.37

Abstract

The presented study has been carried out in order to study the quality of Alkali Treated Cottonii (ATC) made from Eucheuma cottonii which being collected from different regions in Indonesia (Belitung, Nusa Tenggara Barat and Lampung). The quality variables analyzed were the characteristics of raw materials (Clean anhydrous weed and impurities) and the characteristics of ATC produced (moisture content, ash content, acid insoluble ash content, yield, gel strength, sulphate content, gelling -melting point). The analysis was done in 3 replicates and the data were statistically analyzed using SPSS 15 package software. Results indicated that the raw material from Lampung had a better quality than those from Nusa Tenggara Barat and Belitung. In addition, the characteristics of ATC produced from these three raw materials showed that seaweed from Lampung produced better quality ATC than those from Nusa Tenggara Barat and Belitung in terms of their gel strength, sulphate content and yield.
Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber Chasanah, Ekowati; Fawzya, Yusro Nuri; Tarman, Kustiariyah; Januar, Hedi Indra; Nursid, Muhammad
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.269

Abstract

Fatty acids and carotenoid has been known as an anticancer agent on both preventing and treating cancer disease. This study was conducted to analyze the fatty acid profile, carotenoid and in vitro anticancer activity of 12 sea cucumber harvested from Karimunjawa and Lampung waters. The aim of the study was to determin the potency of sea cucumbers as raw material for nutraceutical products. Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. Results of the study showed polyunsaturated fatty acid (PUFA) dominated the composition of fatty acids in the samples from both locations. Holothuria sp. was detected to contain the highest amount of carotenoid. Furthermore, the highest in vitro anticancer activity was detected also in the sample of Holothuria sp. The activity of 30 ppm Holothuria sp. extract against HeLa cell was detected to be almost equal to the 5 ppm doxorubicin control. Concentration of 5 ppm Holothuria sp. extract also showed positive result in killing 50% of MCF-7 and T47D, but capable to 100% kill HeLa and WiDR cells. At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. Statistical analysis showed the amount of carotenoid and two particular fatty acid compounds (docosadienoic and eicosapentaenoic acid) significantly (P<0.05) contributed to the cytotoxic activity that was found in the sea cucumber samples. Those compounds were found in highest concentration from Holothuria sp harvested from Lampung waters, thus being the most prospective raw material for nutraceutical or functional food ingredient with anticancer potency.
Development of enzymatically produced chitooligosaccharide from shrimp industrial waste: opportunity and challenge Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): August 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i2.46

Abstract

Since identified having better properties such as solubility in water and bioactivities comparedto its polymer form, chitooligosaccharide has obtained great attention either from researcher andindustries. Wide application of chitooligosaccharides, from pharmaceutical, food and agriculture,is very depending on the deacetylation degree and the size of oligosaccharide. Enzymaticproduction is more dependable in producing specific, safe and environmentally friendlychitooligosaccharide. Chitosanase is one of chitin degrading enzymes group, having importantrole in production of chitooligosaccharide. Shrimp and crustacean waste which are abundant inIndones ia are important raw material for chitin and its deriv ativ e produc ts inc ludingchitooligosacharide and as a source of chitin degrading enzymes including chitosanase. RCMFPPBhas a c ollec tion of potential c hitin degrading mic robes for produc ing func tionalchitooligosaccharides for food, pharmaceutical and biocontrol applications. There is a bigopportunity for Indonesia as chitooligosaccharide producer as we have relatively big amount ofraw material from shrimp and crustacea’s waste, and local enzyme. One of the problem is thelegality of chitooligosaccharide for food and pharmaceutical products has not been available yet.Another non food application such as biocontrol can be developed first since this product does notneed strict regulation as for food.
TOXICITY OF Aaptos suberitoides EXTRACT ON TIGER SHRIMP Penaeous monodon POST LARVAE Rosmiati, Rosmiati; Suryati, Emma
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 2 (2014): August 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i2.98

Abstract

Marine sponges contain bioactive compounds which can be used not only for human diseases treatment but also for animal diseases treatment. Aaptos suberitoides has been reported to have potential as antibacterial against Vibrio harveyi in vitro. This study aims to study the toxicity effect of Aaptos suberitoides extract on tiger shrimp Penaeous monodon post larvae. The toxicity study of this extract was done by using soaking method. This experiment used Completely Randomized Design with 6 treatments of A.suberitoides extract namely A). 0 ppm, B). 31.3 ppm, C). 62.5 ppm, D). 125, E). 250 ppm, and F). 500 ppm. Each treatment consisted of 3 replications. Findings showed that A.suberitoides butanol extracts at the concentration up to125 ppm were safe for tiger shrimp post larvae. On the other hand, the higher concentration of that gave a toxic effect on the post larvae. The lowest mortality (20.0%) was obtained on the post larvae treated with butanol extract at the concentration of 31.3 ppm and the highest was found at the concentration of 500 ppm (97.0%). Compared to the negative control (0 ppm), there was no any difference exhibited by the post larvae treated with butanol extract at the concentrations of 31.3, 62.5, and 125 ppm. Beside that, these concentrations gave a normal growth and no abnormal behavior. The histological observation did not also show damage on post larvae haepatopancreas tissue.

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