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International Journal of Science and Engineering (IJSE)
Published by Universitas Diponegoro
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Core Subject : Engineering,
The scope of journal covers all area in the application on chemical, physical, mathematical, biological, agricultural, corrossion, and computer science to solve the engineering problems.
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Articles 7 Documents
Search results for , issue "Vol 9, No 2 (2015)" : 7 Documents clear
IDENTIFICATION OF Staphylococcus sp. STRAINS ISOLATED FROM POSITIVE WIDAL BLOOD BASED ON 16S rRNA GENE SEQUENCES Darmawati, Sri
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.721 KB) | DOI: 10.12777/ijse.9.2.97-100

Abstract

The purpose of this study is to identify 8 strains of Staphylococcus genus members isolated from positive Widal blood (4 strains of Staphylococcus saprophyticus, 1 strain of Staphylococcus warneri, 2 strains of Staphylococcus hominis, 1 strain of Staphylococcus xylosus and 1 strain of Staphylococcus capitis) based on 16S rRNA gene sequences. The methods used in this study are conducting PCR of 16S rRNA gene, cloning genes using T-Vector pMD20 which is transformed to E. coli DH5α, sequencing. The results show that four strains (BA 47.4, BA 19.2, KD 29.5 and TG 09.1) are identified as Stap. Saprophyticus strains of Stap. saprophyticus members of ATCC 15305T (99.01-100% similarity). The strain of TG 01.3 is identified as Stap. Warneri strain of TG 01.3 of Stap. Warneri members of ATCC 27836T (99.74-99.93% similarity), 2strains (KT 29.2 and KD 35.1) are identified as of Stap. hominis members of DSM 20328T (99.4-99.67% similarity). The strain of KT 30.5 is not identified
Gas Chromatographic Method: Tool for Rapid and Sensitive Analysis of Residual Solvents in Amoxicillin and Ampicillin Tablets Haque, Sk Manirul; Hossain, S M Zakir; Shanker, Prem
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.184 KB) | DOI: 10.12777/ijse.9.2.90-96

Abstract

A simple and sensitive static head space gas chromatographic (SH-GC) method equipped with FID has been developed and validated for simultaneous determination of residual solvents e.g.,  methanol, dichloromethane and toluene in two therapeutic drugs such as amoxicillin  and ampicillin. The separation was achieved with 30 m long Elite - 5 fused silica capillary column and 0.32 mm inner diameter. The developed SH-GC method offered symmetric peak shape, good resolution and reasonable retention time for all the solvents. Beer’s law was obeyed in the concentration ranges 100 – 1200, 50 – 1000 and 50 – 500 ppm for methanol, dichloromethane and toluene, respectively. The method was validated according to international conference on harmonization (ICH) guidelines in terms of specificity, linearity, precision, accuracy, limit of detection, limit of quantitation, robustness and solution stability. The degrees of linearity of the calibration curves, the percent recoveries, relative standard deviation for the method were also determined. All the validation parameters were within the acceptable range. The developed SH-GC method could, therefore, be suitable for simple and rapid detection of trace levels residual solvents in other pharmaceutical products and thereby it could be used for routine analysis in any analytical laboratory.
The Application of Integrated Multi Trophic Aquaculture (IMTA) Using Stratified Double Net Rounded Cage (SDFNC) for Aquaculture Sustainability Putro, Sapto P.; Widowati, Widowati; Suhartana, Suhartana; Muhammad, Fuad
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1010.788 KB) | DOI: 10.12777/ijse.9.2.85-89

Abstract

The increase of fishery production nationally and internationally may impact on the potential emergence of a variety of environmental problems. The application of sustainable aquaculture is urgently needed by breeding fish for commercial purposes in a manner such that it has a minimum impact on the environment, contributing to the development of local communities and generating economic benefits. The design of the cage and farming practice in aquaculture activities are the important steps to ensure that farming activity is still observed in order to anticipate the risk of organic enrichment caused by the activities. The application of Integrated Multi-Trophic Aquaculture  (IMTA) on the Stratified Double Floating Net Cage  (SDFNC) integrated with biomonitoring are an appropriate solution to the ongoing productive farming practices. IMTA is an aquaculture practice using more than one species of biotas which have ecologically mutual relationship as a part of the food chain in the area at the same time. The application of IMTA allows farmers to get several aquaculture products in the same area without increasing the horizontal area of the farms. At first, the SDFNC has been applied for farming Cyprinus carpio and Tilapia niloticus as polyculture system in freshwater ecosystem of Rawapening Lake, Central Java. Its operation has been able to increase the production capacity of at least 75% of conventional cages. The application of SDFNC-IMTA using milkfish (Chanos Chanos), seaweed (Kappaphycus alvarezii), and white shrimp (Litopenaeus vannamei) has been able to minimize the impact and maintain the water ecosystem in the Gulf Awerange, South Sulawesi.
Nutrient Potency of Rice Straw Processed with Amofer as Cattle Feed Stuff in East Kalimantan Mayulu, Hamdi; Suhardi, Suhardi
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.457 KB) | DOI: 10.12777/ijse.9.2.101-105

Abstract

Forage demand can be supplied from rice straw which processed with certain feed technology innovation. Some advantages can be derived under this technology i.e. increasing nutrient content, optimizing the utilization, improving the efficiency and reducing production cost by minimizing feed cost. Ammoniation fermentation (amofer) as an applied technology could give solution over the limitation of rice straw availability. The objective of this research was to determine the potency of rice straw processed with amofer (amofer-rice straw) as raw material to formulate complete feed. The experiment was carried out with randomized block design with three treatments and six replications. Amofer treatment was carried out by adding urea 3% from the total material and then placed into plastic jar +12 liter and then incubated under an-aerobic process for 18 days. Fermentation material i.e. biology starter by 1% of total material was added at the ninth day. Dry Matter (DM) content resulted from each treatments was 87,28%, 85,96% and 84,61% for T1, T2 and T3, respectively. Crude Protein (CP) content was T1=24,48%, T2=21,04%; and T3=24,46%, Crude Fiber (CF) content was T1=31,30%; T2=31,30%; and T3=31,39%. Total Digestible Nutrient (TDN) for version 1 was  T1=57,29; T2=56,19; and T3=56,89 and version 2 was T1=53,11%; T2=52,28%; and T3=51,10%. The average value of Non Nitrogen Free Extract (NNFE) was T1=23,49%; T2=28,08%; and T3=26,57%. The utilization of amofer as applicable technology is considered as the most appropriate method to increase the quality of rice straw by significantly increase nutrient content in term of crude protein (CP) and reduce crude fiber (CF) content. This increasing quality can be seen from the result of proximate analysis, NNFE and TDN content
Analysis Loading Height of HTR (High Temperature Reactor) Core to Obtain Criticlity of Reactor Setiawati, Evi
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.058 KB) | DOI: 10.12777/ijse.9.2.

Abstract

High temperatur reactor (HTR) attract to be studied due to it has inherent safety characteristics and capabilities to produce energy economically. Design of reactor core in this study is a blend HTR 10 in China with HTR pebble-bed. The reactor has thermal power of 10 MW with inlet and outlet helium temperatures of 250oC and 700oC. HTR design is a cylindrical with helium gas as a coolant and graphite as a moderator. The HTR uses pebble-bed fuel composed a large amount of particles of TRISO in graphite metrics. Kernel radius used to analyse reactor core height in this research is 225 µm with enrichment of 16% in order to achieve critical condition. Reactor criticality is also influenced by the height of active reactor core where pebble-bed fuel is distributed. Calculation of the reactor criticality at any height variations active core is done with MCNP5 modelling techniques. The modelling is done by making the geometry of reactor and pebble-bed which is distributed by using body-centred cubic lattice in the reactor core. From the MCNP5 calculation, the first criticality of HTR can be achieved on the active core height of 150.9012 cm calculated from the bottom active core with criticality value of 1.00312±0.00090. The higher active reactor core is, the more increasing the reactor criticality is. This is occured due to there are many fuel balls of pebble-bed used, so that activity of fission in reactor increases. However, reactor criticality is still in stable condition in each the rise of active core height from critical core height even though reactor reactivity increases 0.01 Δk/k. The minimum of fuel needed to achieve initial criticality (critical core height) is 11,805 pebbles and 8,906 moderators.
CONGESTION AS A RESULT OF SCHOOL AND SHOPPING CENTER ACTIVITY Kumaat, Meike; Mulyono, Agus Taufik; Sjafruddin, Ade; Setiadji, Bagus Hario
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (942.312 KB) | DOI: 10.12777/ijse.9.2.106-112

Abstract

Development of land use in public facilities such as shopping center and school gives an impact on transportation problem in Manado City, North Sulawesi.  To determine factors which have causal relationship with congestion  as a result of school and shopping center activity then it need to be assessed and studied.  Descriptive study with observational survey was used in this study. The study ran Structural Equation Modelling (SEM) by using AMOS program. Estimated method was used to calculate sample size then found 300 repondents, comprised : visitors and mall managers, school visitors, parents, school managers, Public Works department, and urban planning department .The study yielded a statistically significant correlation between  school and shopping center activity with congestion s. The result  indicated that school activity was positively related to congestion with p value  at p=0,000 (p ≤ 0,05). Shopping center activity was positively related to congestion with p value  at p=0,000 (p ≤ 0,05). The closer proximity from school to shooping center will causes severe traffic congestion. The relationship between school facility with proximity was found in p value at  p=0,000 (p ≤ 0,05) . The relationship between shopping center facility with proximity was found in p value at  p= 0,020 (p ≤ 0,05). While, the relationship between proximity with congestion was p= 0,008 (p ≤ 0,05). Monastery school and Mega Mall activity were affecting congestion because a closer proximity of two facilities. This indicates that the occurence of traffic congestion in Monastery School  may be dependent on existence of  Piere Tendean road link
The Efficacy of Dutasteride and Green Tea Combination Towards Angiogenesis and Bleeding on BPH after TURP : Study their effect on VEGF, MVD and Hb Nugroho, Eriawan Agung; Muslim, Rifki; Riwanto, Ignatius; Pranoto, Soetojo Wirjo
International Journal of Science and Engineering Vol 9, No 2 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (978.937 KB) | DOI: 10.12777/ijse.9.2.80-84

Abstract

ABSTRACT Objectives: to find the efficacy dutasteride and green tea in reducing hemorrhage on TURP patients  and to evaluate the anti- angiogenesis effect. Methods: Double Blind Randomized Controlled Trial Post Test Only, with 80 samples, randomized into 4 groups: 1 control group and 3 treatment groups (P1 with 0.5 mg of dutasteride, P2 with a capsul of green tea, and P3 with combination of 0.5 mg dutasteride and a capsul green tea at least 14 days before TURP. We compared the ΔHb define the VEGF expression and MVD count to evaluate the angiogenesis changes between 4 groups. The difference is considered statistically significant with p<.05. Results: ΔHb (-0.40 + 0.246) pg/ml for control group, (-0.20 + 0.067) pg/ml for P1 group, (-0.18 + 0.081) pg/ml for P2 group, and (-0.14 + 0.092) pg/ml for P3 group. VEGF expression were (20.20 + 17.386), (12.90 + 15.509), (11.60 + 9.121), and (3.60 + 1.667) for control, P1, P2, and P3 group respectively. MVD count were (41.20  + 10.273), (32.75 + 9.318), (26.15 + 7.278), and (18.35 + 7.876) for control, P1, P2, and P3 group respectively. The result from between-subject effect tests showed statistically significant differences in ΔHb (p<0.001), VEGF expression (p<0.001), and MVD count (p<0.001). Dutasteride and green tea significantly reduce the hemorrhage during TURP by decreasing the MVD. Conclusion: Administration of  0.5 mg dutasteride and 725 mg of green tea, 14 days prior to TURP significantly reduce the hemorrhage during TURP (Δ Hb) by decreasing the MVD

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