cover
Contact Name
Lianah lianah
Contact Email
Lianah lianah
Phone
-
Journal Mail Official
info@j-tropical-crops.com
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
Journal of Tropical Crop Science
ISSN : 23560169     EISSN : 23560177     DOI : -
Core Subject : Agriculture,
Journal of Tropical Crop Science is an international journal publishing research related to tropical crops. Articles in the journal focus on in-vivo and in-vitro production and production environment, breeding, seed technology, physiology, postharvest, pest and pathology of cereals, vegetables, fruits, ornamentals, medicinal and plantation crops; soil, landscape, turf and agro ecology management under tropical conditions.
Arjuna Subject : -
Articles 1 Documents
Search results for , issue " Vol 1, No 1 (2014): Journal of Tropical Crop Science" : 1 Documents clear
Cloning and Characterization of P5CS1 and P5CS2 Genes from Saccharum officinarum L. under Drought Stress Iskandar, Hayati Minarsih; Widyaningrum, Dwiyantari; Suhandono, Sony
Journal of Tropical Crop Science Vol 1, No 1 (2014): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.987 KB)

Abstract

Increasing world sugar demand might be fulfilled with land extensification which include the use of dry area. Development of drought tolerance and high productivity sugarcane variety could be achieved  by plant genetic engineering. Under drought condition, proline will be accumulated and functioned as an osmoregulator in plant cells. ∆1-pyrroline-5-carboxylate synthase (P5CS) is one of the important enzymes in proline biosynthesis.  This enzyme is encoded by P5CS gene family. We cloned two homologous P5CS genes from sugarcane,  SoP5CS1 (Accession Number : KF178299) and SoP5CS2 (Accession Number : KF178300), which encode 729 and 716 amino acid polypeptides. The identity between these two genes was 74% based on nucleotide sequences. The SoP5CS1 gene had 98% identity with SbP5CS1 (Accession Number : GQ377719.1) and SoP5CS2 had 99% identity with SaP5CS (Accession Number : EF113257.1). In this experiment, sugarcane plantlets  were exposed to medium containing PEG 6000 (40%) for 12, 24, 48, and 72 hours. Proline concentration was measured after treatment and genes expression were analyzed by real time-qPCR. The results showed that the proline concentration was increased 12 folds (9.8 umol.g-1) after 48-hours stress treatment. The highest expression of SoP5CS1 occured at 24-hours treatment with approximately 16 times from plant without PEG (control plant) and decreased gradually at 48 and 72 hours treatment. The highest expression of SoP5CS2 occured at 24-hours drought stress with approximately 3.6 folds compared to control. In drought treatment, the expression level SoP5CS1 was higher than SoP5CS2 and has increased significantly at 12-hours treatment. It is suggested that the SoP5CS1 gene contributes more significantly to the production of proline during drought stress than SoP5CS2. Hence, SoP5CS1 could potentialy be used as a marker to screen sugarcane variety for drought tolerance and for the development of transgenic plant tolerant to drought.Keywords: cloning, drought, expression, P5CS, sugarcane

Page 1 of 1 | Total Record : 1