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Journal of Tropical Crop Science
ISSN : 23560169     EISSN : 23560177     DOI : -
Core Subject : Agriculture,
Journal of Tropical Crop Science is an international journal publishing research related to tropical crops. Articles in the journal focus on in-vivo and in-vitro production and production environment, breeding, seed technology, physiology, postharvest, pest and pathology of cereals, vegetables, fruits, ornamentals, medicinal and plantation crops; soil, landscape, turf and agro ecology management under tropical conditions.
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Articles 5 Documents
Search results for , issue " Vol 2 No 2 (2015): Journal of Tropical Crop Science" : 5 Documents clear
Study of Oil Palm (Elaeis guineensis Jacq) In Vitro Embryogenesis using Young Leaves Explants Wiendi, Ni Made Armini; Constantin, Mondjeli; Wachyar, Ade
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

This study reported in vitro embryogenesis of oil palm using young leaves as explants. Explants were grown in solid modified MS or Eeuwens medium containing different concentrations of NAA and 2,4-D, i.e. media C1, C2, C3, C4 and C5, M1, M2, M3 and M4, to induce embryogenic calli. Compact and pearly-white, globular calli were obtained from the youngest leaf explants 28 weeks after culture.C1 media (MS medium + 107.41 µM of NAA + 100 mg.L-1 of asparagine + 100 mg.L of glutamine-1) produced the highest percentage of calli formation (30.56%), whereas C4 media (C1 supplemented with 67.86 µM of 2,-D ) was the optimal media for embryogenic callus induction. Direct embryoids were obtained from slightly older leaf explants on the C3 media containing NAA after 36 weeks of culture. However, four subcultures using the same medium with gradual reduction of auxin concentration were not successful to develop embryogenic callus and embryoid cells during the course of this study.  
Correlations Between Leaf N, P, K, Ca and Fe Levels and The Production of Metabolites in Torbangun (Coleus amboinicus Lour.) Mulyana, Erik; Aziz, Sandra A; Aisyah, Syarifah Iis; Damanik, M. Rizal Martua
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Torbangun (Coleus amboinicus Lour) is a tropical plant from the Lamiaceae family. Torbangun leaves have a distinct aroma. Torbangun leaves have been traditionally used as a medicinal plant in North Sumatra, Indonesia. Bataknese lactating women in North Sumatra consumed torbangun leaves after giving birth with beliefs that it could increase their breast milk production (lactagogue), and that the leaves can function as anti-fungal and/or anti-bacterial, analgesic, to reduce blood cholesterol, and clean the human uterus. However, scientific evidence on this traditional herb is limited. This research was conducted to: (1) determine the leaf N, P, K, Ca and Fe in different position along the plant and the leaf age as to diagnose the secondary metabolites content of torbangun leaves, and (2) determine the relationship between N, P, K, Ca and Fe leaf concentrations with their metabolite production. The results of the study showed that: (1) the best leaf position and age to determine of the need of N, P, K, Ca and Fe nutrients are on the 3rd leaf position of 5 months-old plant (2) There was a positive correlation between leaf K concentrations with shoot dry weight, Ca with PAL activity, and Fe with total saponins on the 3rd leaf position of 5 months-old plant.Keywords: Coleus amboinicus Lour, torbangun, nutrient, correlation test, secondary metabolites.
The Diversity of Epiphytic Wild Orchids in Mallawa Resort Area of Bantimurung Bulusaraung National Park, South Sulawesi, Indonesia Hiola, St. Fatmah; Dirawan, Gufran D; Caronge, Muhammad Wiharto
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

This research aims to report the diversity of epiphytic wild orchids in Mallawa Resort area of Bantimurung Bulusaraung National Park (BBNP), South Sulawesi, Indonesia. Exploration methods were used in this study to search and record epiphyticwild orchids in this area. The technique of data collection comprised taking pictures with a digital camera for documentation and collecting specimens of wild orchids that were unidentified at the site. The identification of orchid species was conducted by matching the morphology and characterization of epiphytic wild orchids with appropriate photographs showing details to enable identification. The results of the study showed that there were 36 species of epiphytic wild orchids to be found in the study area. The identification to species level included 10 species, there were 17 specimens that were identified to genus level, and seven specimens remained unidentified. Sympodial type orchids dominated the suite of native orchids, with 23 species.Keyword: epiphytes, Mallawa Resort, Bantimurung Bulusaraung National Park, wild orchids
Extraction of Antioxidant Compounds from Rambutan (Nephelium lappaceum L.) Peel as Agricultural Waste in Taiwan Gusman, Josephine Azaria; Tsai, Pi-Jen
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Besides being an agricultural waste, rambutan (Nephelium lappaceum L.) peel has been widely known as good antioxidant source. However, no information about the antioxidant or their way of extraction for rambutan grown in Taiwan can be found. Since its major bioactive compound is geraniin, one of polyphenol compounds, so that total phenolic was used as an index to investigate the optimum condition of extraction from rambutan peel (red and yellow) in Taiwan. Extraction was conducted based on solvent ratio (1:10 to 1:30 g/mL), ethanol concentration (0 to 95 %), ultrasonic extraction temperature (30 to 70°C), and extraction method (conventional from 6 to 36 h at room temperature and ultrasonic from 0.5 to 10 min at 50oC). Results showed that the highest total phenolic content found in red rambutan variety was at 1:15 (g/mL) ratio, but no significant difference for yellow rambutan. Forty percent of ethanolic extraction and 50°C for ultrasonic extraction for both varieties. Conventional method (12 h) and ultrasonic method (2 min) resulted in the highest total phenolic recovery. As to their antioxidant activity in this study, FRAP ranged from 3800.25±86.49 to 4116.5±88.41 (µmol Fe2+/g D.W), flavonoid from 6.41±0.48 to 8.57±0.35 (mg Quercetin/g D.W), and total phenolic recovery from 297.78±4.06 to 358.42±4.63 (mg GAE/g D.W.). This is the first paper regarding the study of rambutan peel in Taiwan.Keywords: Rambutan (Nephelium lappaceum L.), geraniin, antioxidant, agricultural waste
Cloning and Characterization of Partial Chlorophyll a Oxygenase (CAO) Gene Involved in Soybean Shade Tolerance Mechanism Khumaida, Nurul; Kisman, Kisman; Sopandie, Didy
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Plant tolerance mechanism under shade stress is indicated by high chlorophyll-b content, which is synthesized from chlorophyll a by chlorophyll a-oxygenase (CAO) gene. The study was aimed to clone and characterize the partial of CAO gene involved in shade tolerance mechanism in soybean.  Total RNA was isolated from the second trifoliate leaves of soybean plants and the first strand cDNA was prepared using Reverse Transcriptase Moloney Murine Leukemia Virus (RT-M-MLV) (RNase H-). Northern blot hybridization was used to analyze expression of partial CAO gene. The result showed that one partial gene, CAO 3-4 (1,052 bp), comprised of 292 adenine (a), 241 cytosine (c), 276 guanine (g) and 242 thymine (t), therefore the number of amino acid deduction was 338.  The expression of CAO 3-4 partial gene was higher in shade tolerant genotypes than in shade sensitive genotypes, both under 50% shade and dark. These results demonstrated that the CAO gene is involved in shade tolerance mechanism of soybean.

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