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Trianokta Akbar Wardana
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trianokta.akbar@yarsi.ac.id
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Kota adm. jakarta pusat,
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INDONESIA
YARSI Medical Journal
Published by Universitas Yarsi
ISSN : 08541159     EISSN : 24609382     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 6 Documents
Search results for , issue " Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008" : 6 Documents clear
Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in vitro Indra, M Rasjad; Satuman, Satuman; Widodo, Edwin
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.244

Abstract

BackgroundThe aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. MethodsPreadipocyte were isolated from omentum of Rattus norvegicus Wistar using Collagenase type I dan II (Sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% Fetal Bovine Serum (FBS). Insulin were added into the media once the cells attached to the culture plate Proliferating and differentiated cells were counted and analysed by using Oil Red O dan Hematoxylen (HE) staining. Results This study demonstrated that adipocyte could be isolated using Collagenase type I but not type II. The addition of 10% FBSsignificantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. The timing of FBS addition was best performed on day 8 using 10% FBS. Specific adipocyte staining using Oil Red O revealed thatthere were core lipids in mature adipocyte. ConclusionsCollagenase tipe I could be used to isolate preadipocyte cells. Supplementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. Standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro.
KloningOpen Reading Frame (orf) ESAT-6 (Early Secretory Antigenic Target-6) Mycobacterium tuberculosis ke Escherichia coliBL21 (DE3) Agus, Rosana; Maidin, Asaad; Hatta, Mochammad; S.Retnoningrum, Debbie
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.245

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis is an infectious diseases leading to significant death toll in most partof the world. Vaccination with BCG, a TB vaccine, is still a common practice until now. In general, the people in Indonesia receive BCG vaccine during their early childhood, but the efficacy of the vaccine would not last long to adulthood, which allow themto get potential latent TB infection. This latent TB infection might be detected by tuberculin skin test (TST), however, the weakness is that false positive reactions are commonly found due to cross reaction between antibodies produced during BCG vaccination and purified protein derivative (PPD). Alternatively, its detection could be performed by identifying immunodominant antigen to M. tuberculosis. The Early Secretory AntigenicTarget-6 (ESAT-6) for antibody based serological detection with high sensitivity and specificity could also be applied. The purpose of this study was to clone the open reading frame (orf) of ESAT-6 from Mycobacterium tuberculosis into Escherichia coli BL21 (DE3). In this method, orf ESAT-6 was ligated to the expression vector pET-32b and transformed into E. coli BL21 (DE3). Characterization of clones was carried out by cutting the recombinant plasmid using restriction enzymes BamH1 and XhoI. The result showed that three colonies with recombinant plasmid pET-32b-ESAT-6 were obtained. Sequencing of the DNA insert was then performed using the universal T7 primer. Characterization of white colonies with restriction enzymes showed two bands i.e. 288 bp and 5900 bp for orf ESAT-6 and pET32b vector respectively. BLAST analysis of sequence showed 100% homology.
Pengaruh Pemberian Ekstrak Biji Luffa acutangula, Roxb pada Mencit Betina Galur Swiss Webster terhadap Lama Siklus Estrus dan Jumlah Folikel Muda Purwaningsih, Endang
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.250

Abstract

The purpose of thepresent study was to investigate the effect of oral Luffa acutangula, Roxb seed extract respectively in the estrous cycle period and the total number of immature follicles on female mice, Swiss Webster strain. In this study, experimental animals weredivided to 5 treatment groups. The first 3 mice of each group were treated with 200, 300 and 400 mg/kg body weight of seed extract orally. The remaining 2 mice served as treated control (given aquabidest only) and samecontrol. Treatment wih seed extract of each group was given everyday for 20 and 40 days. All female mice whichwere treated with various doses (200, 300 and 400 mg/kg body weight) of Luffa acutangula, Roxb seed extract showed the highly significant different in the estrous cycle period and the number of immature follicles compared to control groups on day 20 and 40. The duration of estrous cycle of the treatment group was longer and the number of immature follicles were decreased; and the duration cycle was longer significant for the 40 day.
Pengaruh Bising Pesawat Udara terhadap Jumlah Sel NK (CD56 + CD16 + CD3 ) pada Masyarakat di Sekitar Bandara Adi Sumarmo Boyolali Hartono, Hartono
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.017 KB) | DOI: 10.33476/jky.v16i3.247

Abstract

Exposure to noise constitutes a health risk. There is sufficient scientific evidence that noise exposure can induce hearing impairment, hypertension and ischemic heart disease, annoyance, sleep disturbance, and decreased school performance. For other effects such as changes in the immune system and birth defects, the evidence is limited.The aims of this study was to find out the effect of the aircraft noise level to the number of Natural Killer Cell (CD56+CD16+CD3) among people in the area of Adi Sumarmo Airport Boyolali.The research finding was expected to contribute to the science development and to give benefits for local government and among people in the Area of Adi Sumarmo Airport in preventing the effect of noise. The research design was an analytical survey with a cross sectional approach, taking location at the Dibal and Gagak Sipat Village, Ngemplak Sub district, Boyolali District. The work was conducted from Juny to October 2008. The number of subjects involved were 57. They were divided into 3 groups; Group 1 was exposed 52.17 dB of noise level (19 respondents); Group 2 was exposed 71.79 dB of noise level (19 respondents); and Group 3 was exposed 92.29 dB of noise level (19 respondents). The samples were taken usingsimple random sampling. The data were analyzed by Anova followed by Post Hoc Test using LSD test completed with Homogenous Subsets. Based on the results of the analysis, a conclusion was drawn that there was a significant effect of the aircraft noise level to the number of Natural Killer Cell (CD56+CD16+CD3) among people in the area of AdiSumarmo Airport Boyolali.
Efektivitas Residu Insektisida Actellic 500EC pada Berbagai Macam Permukaan Dinding Terhadap Kematian Nyamuk Anopheles aconitus, Aedes aegypti, dan Culex quinquefasciatus Boesri, Hasan; Susanti, Lulus
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.446 KB) | DOI: 10.33476/jky.v16i3.252

Abstract

Insecticide spray has been commonly practiced for malaria eradication program. Well chosen and accurate dose of insecticide is required to obtain the best result. In this study, a small scaletrial of Actellic 500EC residual spray with dosage ranges of 1.0 ml/m2, 2.0 ml/m2, 4.0 ml/m2 and 5.0 ml/m was applied on wall, shelf and bamboo surfaces. Three malaria vectors e.g. Anopheles aconitus, Aedes aegypti, andCulex quinquefasciatus mosquitoes, 3-4 days old and well fed were used for the experiment using bioassay test kit cone (WHO standard 1975). The result showed that 4.0 ml/m dosages of Actellic 500EC were more effective than the other dosages. The mosquito mortality was 100% in one week for An. aconitus, Ae. aegypti and Cx. quinquefasciatus.
Kandungan Vitamin A dan Protein Bakso Belut di Daerah Istimewa Yogyakarta Suryani, Yoni; Yuliati, Yuliati; Farida, Yuyun
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.248

Abstract

The objectives of this study were to investigate vitamin A and protein contentsin eel (Monopterus albus) meat balls. Theeel meat balls were made byaspecial addition of tapioca30%, 40%, 50% and 60% of thetotal weight of eel meat. Hedonic test was conducted in order to find the levels of panellist preferences. Friedman test wasemployedto find the effect of tapioca addition on colour, flavour, texture and taste. The content of vitamin A was analysedusing HPLCmethodand proteincontentusing Micro Kjeldahl method. The result revealed that the variation of tapioca addition gavenosignificanteffect on colour performance with the significance level of 1.0. However, it gave significant effect on flavour, texture and taste preferences with the significance level of <0.05. It wasfound that the most favourite eel meat balls was the one with tapioca addition of 50% of the eel meat weight. The average of vitamin A contentwas 2,215.95mg/100 gand protein is 7.2896 mg/100 g on the most favourite eel meat balls.

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