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Contact Name
Gilang Nugraha
Contact Email
gilang@unusa.ac.id
Phone
+6282233441232
Journal Mail Official
ijmlst@unusa.ac.id
Editorial Address
Kota Surabaya, Jawa Timur, Indonesia
Location
Unknown,
Unknown
INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
ISSN : 26846748     EISSN : 26569825     DOI : doi.org/10.33086/ijmlst
Core Subject : Health, Science,
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
Arjuna Subject : -
Articles 18 Documents
BLOOD LEAD CONCENTRATIONS AND THE NEUROPSYCHOLOGY SCORES OF PREGNANT WOMEN IN KLANG VALLEY, MALAYSIA Shamsudin, Shamsul Bahari; Hashim, Jamal Hisham; Ismail, Nik Nasri Nik; Koentjoro, Maharani Pertiwi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (941.974 KB) | DOI: 10.33086/ijmlst.v2i1.1499

Abstract

Pregnant women with high blood lead posed high risk to their fetus as placental transfer can occurs to the fetus. The objective of this study was to identify the relationship between blood lead and the neuropsychological score of women who were in their 3rd trimester of pregnancy. These respondents were undergoing a routine antenatal checkup at a teaching hospital located in Klang Valley areas.  Blood lead concentrations were analyzed using graphite furnace Atomic Absorption Spectrophotometer (AAS). The neuropsychological scores were measured with WHO Neurobehavioral Core Test battery (NCTB). The test consists of 7 items, which made up of the Digit Symbol, Trail Making, Digit Span, Benton Visual Retention Test, Pursuit Aiming, Santa Ana Manual Dexterity, Reaction Time and Movement Time tests. The mean blood lead was 7.78±4.77 µg/dL. The mean score for the total NCTB test was 50.00±5.24. Statistical analysis showed blood lead concentrations were inversely correlated with the total NCTB score (r= ?0.462, p?0.01). The correlation was about 21.3%. The General Linear Model (GLM) showed that age (?= ?0.15, p=0.017), weight (?=2.67, p=0.05) and height (?=?1.97, p=0.05) also influence the total neuropsychological scores. In conclusion, blood lead reduces the total neuropsychological scores. The scores for each of the 7 items were inversely and significantly correlated with blood lead concentrations except for the Trail Making and Santa Ana Manual Dexterity tests.
CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Andini, Ary; Prayekti, Endah; Dyah Wulandari, Devyana; Nidianti, Ersalina
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.839 KB) | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the  best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing  sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into  K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained  collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (ENHALUS ACOROIDES) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY Orno, Theosobia Grace; Rantesalu, Agnes
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.391 KB) | DOI: 10.33086/ijmlst.v2i1.1463

Abstract

The studies analysing the use of natural ingredients as an alternative treatment in the field of pharmacology are developing very rapidly. One of researches that is quite promising in the pharmaceutical industry is the application of marine materials. Marine materials that are frequently used consist of shellfish, algae, sponges and seagrass. Several studies on toxicity tests have shown that the methanol extract of seagrass (from species Enhalus acoroides) is more toxic than the other seagrass family. This study aims to test the toxicity level of Seagrass (E. acoroides) extract from Soropia coastal waters. The research method in this study was an experimental laboratory using E. acoroides Seagrass as a sample that was obtained from Soropia Coast, Konawe Regency, Southeast Sulawesi Province. The sample was extracted using methanol as a solvent by macerating it and was tested for its toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test results showed that the samples with a concentration of 10 ppm, 100 ppm and 1000 ppm in leaves extracts produced an LC50 value of 404.88 ppm, while the stem and root extracts has a value of LC50 >1000 ppm. The test was continued with higher concentration of leaves extracts consisted of 250 ppm, 500 ppm, and 1000 ppm. The toxicity test showed an LC50 value of 0.7309; which means that it was very toxic. The methanol extract of Seagrass (E. Acoroides) is potential to be used for further analysis and anticancer formulations.
LABORATORY TRIAL OF PROTEIN DETERMINATION IN URINE USING DIFFERENT PH VALUES OF ACETIC ACID AND ACETATE BUFFER METHOD Rahayu, Dinar; Rustiana, Tuti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1041.078 KB) | DOI: 10.33086/ijmlst.v2i1.1459

Abstract

Abstract The determination of protein in urine is important in clinical examination along with other parameters in urine. The presence of protein in urine can be interpreted that there is a disorder in kidney. Acid and heat coagulations method is still widely used in many areas to determine protein in urine. In this method, the characteristic of protein that will precipitate in the presence of acid or if exposed to heat is deployed to gain information about the amount of protein. The greater amount of protein, the more prominence is the coagulation. Urine pH also varies according to the condition, classic acidosis will give an acidic urine and the presence of ammonium producing bacteria can cause basic urine. In this research acetic acid method with 6% of CH3COOH and pH value of 2,9 and buffer acetic with pH 4,5 are used to determine the certain amount of protein (+3 value, corresponds with 2-4 mg/dL protein in urine) in varied pH values of urine samples. To compare the results, first in control urine with pH 6,8 the results of both methods is compared and shows no significant different, then the Kruskall-Wallis test is used to compare the results in other pH values to control and the test is shown also there are no significant difference. This shows that either acetic acid at pH 2,9 or acetic buffer at pH 4,5 can be used to determine protein amount in urine.
A POWERFUL ELISA TECHNIQUE TO TEST THE POTENTIAL OF EXTRA VIRGIN OLIVE OIL IN REDUCING TNF-Α LEVEL AND EDEMA VOLUME IN MALE RATTUS NORVEGICUS EXPOSED TO CARRAGEENAN Aulia, Andina Putri; Maat, Suprapto; Aryati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.433 KB) | DOI: 10.33086/ijmlst.v2i1.1400

Abstract

Extra virgin Olive oil is extracted from fruit that can be used as anti-inflammatory agent. This research aimed to test the potential of extra virgin olive oil in reducing edema volume and TNF-? plasma in carrageenan-induced rats. This research was purely experimental research with the post test control group design. A total of twenty eight Wistar rats were divided randomly into four treatment groups. Group I was a control negative group, while the group II, III, and IV were orally administered with extra virgin Olive oil at the dose of 0.9 ; 1.8 ; 2.7 mL/day, respectively. Paw edema was measured one hour before the rats was induced to carrageenan and every hour until four hours after it was induced to carrageenan. TNF-? plasma was measured at four hours. Analysis of the data was done by calculating the presentation of edema inhibition in every group, then the data was statistically analyzed by Anova, Repeated Anova, LSD and Kruskal Wallis test with 95% confidence interval. The result showed that extra virgin olive oil has an anti-inflammatory effect. The highest decrease in edema volume percentage was in group III (14.21%). There was a significant difference in the edema volume of all treatment groups at each time of the experiment with TNF-? value (p < 0.05). In conclusion, the administration of extra virgin olive oil can lower the volume of carrageenan-induced edema in rats depend on the dose. Also, the administration of extra virgin olive oil can be dose-dependent in reducing the levels of TNF-? in carrageenan-induced edema in rats.
STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE Febryani, Noor; Amalia, Ikke Nanda; Anggraeni, Intan Dwi; Nugraha, Gilang
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.412 KB) | DOI: 10.33086/ijmlst.v1i2.1311

Abstract

Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test.
THE EFFECT OF ENVIRONMENTAL FACTOR AND USE OF PERSONAL PROTECTIVE EQUIPMENT ON THE SYMPTOMS OF ACUTE RESPIRATORY TRACT INFECTIONS IN FURNITURE INDUSTRY WORKERS Sunaryo, Merry
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.775 KB) | DOI: 10.33086/ijmlst.v2i1.1307

Abstract

Dust is one type of potential hazardzs in the workplace that can affect the health of the workers. The occupation that are always exposed to dust is furniture industry workers so that they have higher risk of getting acute respiratory tract infection (ARI) disorder which can interfere with breathing. The wood dust is formed from some of the sawn wood and sanding that will lead to an acute respiratory tract infection. The study aimed to determine the effect of environmental factor and the use of Personal Protective Equipment (PPE) against the symptoms of acute respiratory infections in the furniture industry workers. The research method used was quantitative method with observational and cross-sectional research types and it was analysed by using logistic regression test. The population in this study was the workers of the furniture industry at Semarang street, Surabaya City, with a total of 57 people, of which 37 furniture workers as a sample. The results show that most of the workers has symptoms of acute respiratory tract infection. It could be influenced by the environmental factor such as dust exposure that produced wood dust in each manufacturing processes. Additionally, the use of PPE also affected the occurrence of acute respiratory tract infections symptoms in the workers. In conslusion, many factors can influence the occurrence of acute respiratory tract infection symptoms in the furniture industry workers. Therefore, it is necessary to minimize the dust exposure in workers by wearing PPE such as respirators.
EVALUATION OF ERYTHROCYTE SEDIMENTATION RATE (ESR) OF MOTORCYCLE WORKSHOP WORKER EXPOSED TO BENZENE Sahri, Moch.; Koentjoro, Maharani Pertiwi; Shamsudin, Shamsul Bahari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.29 KB) | DOI: 10.33086/ijmlst.v1i2.1303

Abstract

Occupational exposure to benzene from fuel oil in workshop motorcycle workshop causes several health effects, depending upon the level and duration of exposure. Analysis of blood is important to access the status of worker? health. The study aimed to assess the gather basic information required for protecting workers? health and improve working conditions in the works sites by investigating the ESR levels. A comparative cross-sectional study conducted in Surabaya City, East Java-Indonesia, which involved 100 workers. The occupational data collected using a structured questionnaire while blood analysis parameters measured with an automated hematology analyzer.   The result showed that leukocytes, erythrocytes, hemoglobin (HGB), hematocrit values (HCT), platelet, MCV, RDW, eosinophil, basophils, neutrophil rods, neutrophil segment, lymphocyte, monocytes were in normal value. Whereas, MCH, MCHC, ESR has shown in abnormal value as average 24.5 pg, 29.18 g/dL and 8.21 mm/h, respectively. Those value, especially in ESR value indicated the increasing concentration plasma viscosity. This is may cause by inflammation in the workers? body. To prevent the hazardous effect of benzene exposure, occupational health should be implemented for workers in order to protect them from exposure to benzene.
IDENTIFICATION OF ASCORBIC ACID CONTENT IN CARICA PAPAYA L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY Riscahyani, Nosy Maria; Ekawati, Evy Ratnasari; Ngibad, Khoirul
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.857 KB) | DOI: 10.33086/ijmlst.v1i2.1291

Abstract

Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.
THE EFFECT OF BEETROOT (BETA VULGARIS L.) JUICE ON CHOLINESTERASE ACTIVITY IN FARMERS EXPOSED TO ORGANOPHOSPHATE PESTICIDES Wulandari, Devyana Dyah; Santoso, Andreas Putro Ragil; Wulansari, Devyani Diah
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.943 KB) | DOI: 10.33086/ijmlst.v1i2.1215

Abstract

A decrease in cholinesterase (chE) enzyme activity is an indicator of pesticide poisoning, especially organophosphate pesticides. The chE activity reduction will result in an increase in the amount of acetylcholine and will bound to muscarinic and nicotinic receptors in the central and peripheral nervous system which can lead to the onset of Alzheimer's disease. However, antioxidant products can slow the progression of Alzheimer's disease by protecting neurons from oxidative stress. Red Beetroot (Beta vulgaris L.) contains betalains and phenolic compounds which act as an antioxidants that are capable of preventing such illness. This study aims to prove the concept that antioxidant compounds in red beetroot juice can increase the chE activity in subjects exposed to organophosphate pesticides. This research is an observational study with an experimental approach. This research used 25 farmers who were exposed to organophosphate pesticides as respondents. They were given 500 ml of beetroot juice twice a day for 2 consecutive months. Cholinesterase levels were measured before and after consuming red beet juice. Measurement of cholinesterase levels was carried out using a kinetic photometric test. This method was developed based on recommendations from the German Society of Clinical Chemistry (DGKC). The results showed that the average of chE (U/L) Level Before Treatment was 8.102 and 8.380 after treatment with a p value 0,62. It can be concluded that there was an increase in the level of cholinesterase but it was not statistically significant. This may be caused by a different response to activity of cholinesterase after consuming beetroot juice in each subjects, age difference, smoking habits, and personal protective equipment.

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