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Jurnal ILMU DASAR
Published by Universitas Jember
ISSN : 14115735     EISSN : 24425613     DOI : -
Core Subject : Science, Education,
Jurnal ILMU DASAR (JID) adalah jurnal ilmiah yang melingkupi bidang matematika, fisika, kimia dan biologi. Naskah yang diusulkan untuk diterbitkan Jurnal Ilmu Dasar adalah naskah yang belum pernah diterbitkan dan atau tidak sedang dipertimbangkan penerbitannya di majalah lain. Jurnal Ilmu Dasar menerima tulisan hasil penelitian dasar (bukan aplikasi) untuk bidang matematika, kimia, fisika, dan biologi. Naskah ilmiah ditulis dalam bahasa Indonesia atau dalam bahasa Inggris yang baik. Secara lebih detail dapat dilihat dari pedoman penulisan.
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Articles 8 Documents
Search results for , issue "Vol 16 No 1 (2015)" : 8 Documents clear
INDUCTION SOMATIC EMBRYOGENESIS USED 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) AND KINETIN IN SPINDLE LEAF EXPLANT SUGARCANE Sholeha, Wardatus; Sugiharto, Bambang; Setyati, Dwi; Dewanti, Parawita
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1384.105 KB) | DOI: 10.19184/jid.v16i1.1387

Abstract

Induction of somatic embryogenesis in sugarcane requires the composition Plant Growth Hormone (PGH) appropriate. Utilizing of PGH (2,4-D and kinetin) is expected to induce sugarcane somatic embryogenesis. The purpose of this study was to obtain the concentration of 2,4-D and kinetin that effective for the multiplication of sugarcane var. NXI 1-3 through somatic embryogenesis. This study consists of four stages: callus induction, callus proliferation, regeneration of shoots and encapsulation. The plant material used is a spindle leaf sugarcane var. NXI 1-3. Callus induction used 2,4-D with different concentration (2 ppm, 3 ppm and 4 ppm). Callus proliferation used 2,4-D with concentration 1 ppm and 2 ppm. Regeneration of shoots used kinetin 0,5 ppm. The results are showed that the optimal induction of embryogenic callus on medium MS + sucrose 30 g / L + CH 300 ppm + 300 ppm PVP + 2,4-D 4 ppm as indicated by the high percentage of explants forming embryogenic callus that is equal to 40% and the respective concentration 2 ppm and 3 ppm is 33,3% and 37,5%. In proliferation stage, the development callus optimal on medium MS + sucrose 30 g / L + CH 300pm + PVP 300 ppm + 2,4-D 2 ppm and formulations for regeneration shoot on medium MS + sucrose 30 g / L + kinetin 0.5 ppm. The result of encapsulation can be shaped 100 sythetic seed. Keywords: Somatic embryogenesis, spindle leaf, kinetin, 2,4-D
ISOLATION OF GENES ENCODING ARTHROPOD ODORANT BINDING PROTEINS (OBP), D7 FROM SALIVARY GLAND VECTORS OF MALARIA: ANOPHELES SUNDAICUS Pawana, Nuryatmaja Gora; Muzakhar, Kahar; Senjarini, Kartika
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v16i1.621

Abstract

The isolation of Arthropod Odorant Binding, D7 protein, encoding genes from Anopheles sundaicus and An. maculatus mosquitos as the malaria vectors in Indonesia is necessary to recognize their characteristic. The isolated genes can be used to develop the Transmission Blocking Vaccine (TBV). This research aims to characterize the D7 protein encoding genes from An. sundaicus and An. maculatus through the synthesis of complementary DNA (cDNA) of D7 protein by using D7 protein primer that has been used for the other species of Anopheles. The mosquitos were taken from Dusun Parasputih, Bangsring, Wongsorejo, Banyuwangi, Jawa Timur. Isolation of the salivary gland was done by performing microdisection method and the isolation of the total RNA was done by performing High Pure RNA Isolation Kit (Roche-Germany). Synthesis of cDNA D7 encoding gene and its amplification were performed by using Maxime RT-PCR Premix Kit (iNtRon Biotechnology). The result of the total RNA and RT-PCR were run  in agarose gel and visualized under the UV transiluminator. Based on the visualization, we found that the salivary gland total RNA of female An. sundaicus was 500-750 base pair (bp). The RT-PCR visualization showed a band sized below 100 bp and it was concluded not to be the size of the D7 protein encoding gene. An incompatibility of D7 primer from An. gambiae with cDNA template from An. sundaicus was suspected to be the reason of the gene isolation failure.Keywords: gene isolation, D7 protein, salivary gland, Anopheles sundaicus
THE DIVERSITY OF HOLOTHURIOIDEA IN THE INTERTIDAL ZONE OF THE PANCUR SHORE OF THE ALAS PURWO NATIONAL PARK Afrely, Riya Wulan; Rosyidi, Moh. Imron; Fajariyah, Susantin
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.227 KB) | DOI: 10.19184/jid.v16i1.1390

Abstract

Holothurioidea well known as Sea Cucumber is one of the classes from the Echinodermata phylum. Sea cucumbers are benthic animals that move slowly. Currently they already identified about 1135 species. Their existence usually associated with abiotic factors such as water salinity, temperature, pH, wave motion, light intensity and the substrate. The ability of the Holothurioidea to adapt to the abiotic factors will affect species diversity. The research aims is to determine the species diversity in the intertidal zone at Pancur Shore of the Alas Purwo National Park. It includes the species composition, species diversity index and the index of equality The study was conducted on 14th-21st of June 2014, by using systematic plotting transect method. The results found: one order, one family, two generas and eight species. The Species diversity index of Holothurioidea at Pancur Shore is low (0.930) and the equality index  classified uneven (0.447).Keywords: Holothurioidea, intertidal, diversity, evennes
EPHYPITIC FERNS (PTERIDOPHYTA) FROM RAUNG MOUNT BANYUWANGI, EAST JAVA INDONESIA Ulum, Fuad Bahrul; Setyati, Dwi
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1400.413 KB) | DOI: 10.19184/jid.v16i1.1486

Abstract

An investigation of epiphytic Pteridophytes diversity at Mount Raung, Banyuwangi, East Java Province, Indonesia, was carried out in 7 February 2015. As many as 11 Pteridophytes from 3 families had been identified based on their morphological chracteristic. Among them, four species of Family Aspleniaceae, including Asplenium confusum Tardieu & Ching, Asplenium nidus L., Asplenium salignum Blume, and Asplenium tenerumoides S.B.Andrews. Polypodiaceae include Leptochilus wrightii (Hooker & Baker) X. C. Zhang, Microsorum punctatum (L.) Copel., Pyrrosia bicolor ( Kaulf. ) Ching, and Schellolepis percussa (Cav.) Pic.Serm. Vittariaceae include Antrophyum formosanum Hieron., Vittaria ensiformis Sw., and Vittaria lineata (L.) Sm. which is the smallest specimen with less than 10 cm long.Keywords: Mount Raung, Pteridophyta, Epiphytic, Families. 
X-RAY PHOTOELECTRON SPECTROSCOPY (XPS) ANALYSIS OF UNDOPED ZNO AND ZNO:ER THIN FILMS Sugihartono, Iwan; Budi, Esmar; Budi, Agus Setyo
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.446 KB) | DOI: 10.19184/jid.v16i1.1052

Abstract

Undoped ZnO and ZnO:Er  thin films were deposited on p-type Si substrates by ultrasonic spray pyrolisis (USP). Undoped and ZnO:Er thin films have been analyzed by using X-ray Photoelectron Spectroscopy (XPS). The results show that the XPS spectrum has two Er peak at ?157 eV and ?168 eV. The XPS Zn 2p spectrum of undoped ZnO and ZnO:Er thin films have binding energy for Zn 2p3/2 (~ 1021 eV) and Zn 2p1/2 (~1045eV) were found no shift in binding energy after the incorporation of Er. Meanwhile, after Er incorporates into ZnO, the O 1s spectrum is composed two peak of binding energy (BE) at ~530.5eV and the shoulder about 532.5 eV.Keywords: ZnO thin films, ZnO:Er, XPS, binding energy
BIOLOGICAL CONTROL ENTOMOPATHOGENIC NEMATODES HETERORHABDITIS SP. AND STEINERNEMA SP. PEST CONTROL TERMITE LAND AS COPTOTERMES SP. AND MICROTERMES SP. IN THE DISTRICT LUMAJANG Qodiriyah, Qodiriyah; Sulistyanto, Didik; Purwatiningsih, Purwatiningsih
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.051 KB) | DOI: 10.19184/jid.v16i1.1518

Abstract

This research aims to find out the most effective concentration (LC50) and the most effective time (LT50)  and  to select a biological agent netamoda of nematodes Steinernema sp. dan Heterorhabditis sp. as the environmentally friendly biological control agents on the ground termite Coptotermes sp. and   Microtermes sp. in Lumajang. All the data were analyzed by using the termite mortality percentage variance analysis. The LC50 and LT50 were tested by using the probit analysis. Therefore, the empirical probit values were obtained from the percentage of mortality after they were calculated by the Abbot formulation. The biological agents netamoda entomopathogenic Heterorhabditis sp. and Steinernema sp. had the high value on the pathogenicity of Coptotermes sp. and Microtermes sp., the LC50 values on Coptotermes sp. of nematodes Heterorhabditis sp. was 14.94 IJ/ml and Steinernema sp. was 15.22 IJ/ml., the LC50 values on Microtermes sp. of nematodes Heterorhabditis sp. was 16.54 IJ/ml and  Steinernema sp. was 20.39 IJ/ml., the LT50 values on Coptotermes sp. of nematodes Heterorhabditis sp. was 1.29 hours and Steinernema sp. was 1.35 hours, and the LT50 values on Microtermes sp. of nematodes Heterorhabditis sp. was 2.14 hours and Steinernema sp. was 1.82 hours. Through pathogenicity test, the entomopathogenic nematodes Heterorhabditis sp. is more effective to control subterranean termites compared with the nematode Steinernema sp. This happens because Heterorhabditis sp. are actively engaged and looking for a host so that there are a large number of possibilities of contacting them with a very large subterranean termites.Keywords: Entomopathogenic nematodes,   Heterorhabditis sp., Steinernema sp., LC50, LT50 
ONE DIMENSION MODEL BASED FILTER FOR SEPARATION OF TIME-LAPSE MICROGRAVITY ANOMALY Minardi, Suhayat; Ardianto, Teguh
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v16i1.1055

Abstract

The research has a goal to develop a model-based filter to separate the time lapse microgravity anomali caused by the 3 pieces of the source of anomalies with different depths. The activities which included in this research are: geological modeling, calculation of time lapse microgravity responses caused by several sources, designing of digital model based filter, application of the filter to separate synthetic time lapse microgravity anomali data, and performance test of the filter. Designed model is multi aquifer sistem with homogeny isotropic properties at different depth. Equipment of this research is a unit computer with MATLAB Version 7.11.0.584 (R2010b) and Microsoft Excel 2007 software. The designed filter can separate time lapse microgravity anomali based on its sources successfully with error less than 5%.Keywords: Filter, synthetic, time lapse microgravity anomaly 
STRUCTURAL EQUATION MODELING OF THE FACTORS AFFECTING THE NUTRITIONAL STATUS OF CHILDREN UNDER FIVE IN BANYUWANGI REGION USING RECURSIVE (ONE-WAY) GSCA Tirta, I Made; Susanti, Nawal Ika; Dewi, Yuliani Setia
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1517.157 KB) | DOI: 10.19184/jid.v16i1.534

Abstract

Structural Equation Modeling is one among popular multivariate analysis, especially applied in pschology and marketing. There are two main types of Structural Equation Modeling namely covariance-based or CB-SEM and variance-based or Partial Least Square (PLS)- SEM. Both types have advantages and disadvantage. To overcome its limitation, Generalized Structured Component Analysis (GSCA) was then proposed as an extension of PLS-SEM. In estimating the parameters, GSCA uses Alternating Least Squares (ALS) and in estimating the standard error of the parameter estimates it uses the bootstrap method. In this paper, GSCA is applied to study the causality model of Infant nutritional status, in relation with socio-economic status and infantcare status in Banyuwangi Region. The results show that both socio-economic and infantcare status have significant positive influence on infant nutritional status.Keywords:  Alternating least square, generalized structural component analysis,  nutritional status of infants,  structural equation modelling

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