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INDONESIA
Jurnal ILMU DASAR
Published by Universitas Jember
ISSN : 14115735     EISSN : 24425613     DOI : -
Core Subject : Science, Education,
Jurnal ILMU DASAR (JID) adalah jurnal ilmiah yang melingkupi bidang matematika, fisika, kimia dan biologi. Naskah yang diusulkan untuk diterbitkan Jurnal Ilmu Dasar adalah naskah yang belum pernah diterbitkan dan atau tidak sedang dipertimbangkan penerbitannya di majalah lain. Jurnal Ilmu Dasar menerima tulisan hasil penelitian dasar (bukan aplikasi) untuk bidang matematika, kimia, fisika, dan biologi. Naskah ilmiah ditulis dalam bahasa Indonesia atau dalam bahasa Inggris yang baik. Secara lebih detail dapat dilihat dari pedoman penulisan.
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Articles 12 Documents
Search results for , issue "Vol 8 No 2 (2007)" : 12 Documents clear
FUNCTIONALIZATION OF POLYETHYLENE FILMS WITH PH-RESPONSIVE ACRYLIC ACID USING GRAFTING TECHNIQUE INDUCED BY UV-RAY Suka, Irwan Ginting; Simanjuntak, Wasinton
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

This research was carried out to study photografting of acrylic acid onto linear low-density polyethylene (LLDPE) film, induced by UV ray at 60°C, as a function of monomer concentration and irradiation time.  This research was conducted since it has been known that hydrogels formed by polyacrylic acid exhibit shrinking and swelling behaviors according to the change in pH of the aqueous medium. Therefore, the acrylic acid-grafted LLDPE film is expected to exhibit a pH-responsive function, in which it swells in alkaline media (pH = 8) and shrinks in acidic media (pH = 4).  Xanthone was used as a photoinitiator, which was coated on the film earlier. The samples were characterized using attenuated total reflection infrared spectroscopy and a scanning electron microscope analysis. The pH-responsive experiments revealed that the samples exhibited the pH-responsive characters, as expected, in which they shrank and swelled in acidic and alkaline media, respectively. The pHresponsive character was higher for the samples prepared in the system containing monomer at higher concentration. The different extents of the pH-responsive character of the resulting grafted PE films were discussed in terms of location of grafted chains in the film substrate.
PRODUCTION AND CHARACTERIZATION OF ENZYME β-ENDOXYLANASE FROM BACTERIA OF TERMITE-INTESTINAL SYSTEM Ratnawati, A. A. Istri; Handayani, Wuryanti; Puspaningsih, Ni Nyoman Tri
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

A mesophile bacteria isolated from intestinal systems of local-soil termite, produced an extracellular ?-endoxylanase upon growth on xylan. Optimum production of the enzyme was found in media containing oatspelt xylan at 37oC after sixth hours. The activities of its ?-endoxylanase on oat-spelt xylan was investigated. It had an optimum pH and temperature, 5.0 and 40o C, respectively. However, pH stability occurred between 5.08.0. The enzyme was stable at 40o C for four hours and possessed a half life of four hours. ?-endoxylanase had an apparent molecular mass of 45.000 to 66.200 Dalton as determined by SDS-PAGE. Analysis of zymogram using SDS-Xylan-PAGE indicated that enzymes could degrade oat-spelt xylan as substrates.
CHROMATOGRAM AND SPECTRAM PROFILES ANTIMALARIAL ISOLATE FROM DICHLOROMETHANE EXTRACT OF ARTOCARPUS CHAMPEDEN SPRENG. STEM BARK Nuri, Nuri
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The isolate which possessed antimalarial activity had been isolated from dichloromethane extract of Artocarpus champeden Spreng. Stem bark. The isolate inhibited Plasmodium falciparum growth in vitro by IC 0.024±0.011 ?g/ml. Chromatogram and spectram of isolate analysis showed that it contained flavonol as a  major compound.
EFFECT OF SUBSTITUTING PURE SUCROSE BY SUGARCANE JUICE AS CARBON SOURCE ON THE FERMENTATION OF DEXTRAN PRODUCTION Triantarti, Triantarti; Santoso M, Hendro
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Sucrose is a carbon source for dextran fermentation and it is also used as a substrate of dextransucrase enzyme for producing dextran.  Sugar cane juice containing sucrose as a main sugar, hence it is potential to be used as a cheap medium for dextran fermentation.  This research was conducted to study the dextran fermentation using sugar cane juice as a medium. Two main experiments were done in this research. The first experiment has been carried out to determine the optimum medium composition for dextran fermentation using pure sucrose as a carbon source by variations on type and concentrations of yeast extract and buffering minerals.  The second experiment was conducted to determine the effect of substituting pure sucrose in the fermentation medium by sugar cane juice. Fermentation was conducted at static condition, room temperature and 16-20 h fermentation time. The results showed that the optimum conditions for dextran fermentation using pure sucrose were sucrose 20%, yeast extract 0.75% (technical grade yeast extract was able to be used) and K2HPO4 minerals for buffering medium. Dextran production was able to reach 51 mg/g medium. The optimum medium composition and fermentation conditions were used as a control medium.  In the second experiment, pure sucrose in the control medium was substituted by sugar cane juice with variations of 0; 50, 75 and 100%. Technical grade yeast extract was still added at 0.75%.  The result showed that the higher sugar cane juice concentration the lower dextran production in the fermentation.  On the other hand, medium fermentation containing 100% sugar cane juice without yeast extract was able to produce 45 mg dextran/g medium, which was not significantly different to dextran production from control medium.  This experiment showed that sugar cane juice was a potential material as a cheap carbon source for dextran fermentation
GROWTH OF BACTERIA ISOLAT CONSORTIUM FROM BENAKAT ON HIGH SALAINITY CRUDE OIL MEDIA Nugroho, Astri; Effendi, Edison; Annisa, Fiona
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The production and cunsumption of oil and petroleum product are increasing worldwide and threat of oil pollution is increasing accordingly. About 10 million tons of oil and petroleum products are used worldwide each day, it will occur 5% of all oil pollution entering the sea. For that reason, needs a treatment to handle and remediate aquatic ecosystem like former. Bioremediation is a process to detocsify dan degrade crude oil. Expected from this process, land and aquatic environment where were polluted by petroleum can be natural. Treatments are divided into three stages, which are (1) isolation of dominant microorganism that degraded petroleum hydrocarbon in 15% salinity and TPH 10%, (2) Identification of bacteria with biochemistry tes and Analytical Profile Index 20 NE test (3)Biodegradation ability test in 15%, 10% salinity and 30? (seawater salinity) and TPH (Total Petroleum Hidrocarbon) 1%, 2.5 %, 5 %, and 10 %. The microorganisms that were identified from the isolation crude oil from Benakat are Pseudomonas aeruginosa, Aeromonas hydrophila and Agrobacterium radiobacter. Total sel microorganisms/ml in exponential phase are P. aeruginosa: 2.42E/ml, A. hydrophila: 2.99E+14 /ml, A. radiobacter: 2.42E+14/ml, and mixed culture microorganism: 4.65E/ml. The average percentage sequence degradation from the optimum  growth are mixed culture = 92%, P. Aeruginosa = 90%, A. hydrophila = 88%, and A. radiobacter = 79%. The percentage sequence degradation of mixed culture in 15% salinity and 1%, 2,5%, 5%, 10% TPH concentration are 90%, 84%, 97%, 94%. Otherwise, 30? salinity and 1%, 5%, 10% TPH concentration, the percentage of biodegradation are 98%, 96%, 84%.
CONTINUM REGRESSION WITH DISCRETE WAVELET TRANSFORMATION PREPROCESSING Setiawan, Setiawan; Anwar Notodiputro, Khairil
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In the multiple regression modeling, serious problems will be occurred if  independent variables are correlated, to be named  ill conditioned problem, and the number of observations is much less than the number of independent variables, it is a singularity problem. Continum Regression (CR) approach, it?s better to overcome the problem of ill conditioned, but if  the number of observations is much less than the number of independent variables usually facing the problem in computing. So the first step, it needes dimension reduction of independent variables (known as a preprocessing method). Discrete wavelet transformation (DWT) is one of a good method handle the problem of singularity.  The research we have studied  combination of CR and DWT as a preprocessing method can solved the problems of ill conditioned and singularity.  The result of empirical research with simulation data has concluded that performance of CRDWT have very good potency to overcome the problems of the number of observations much less than the number of independent variables and ill conditioned.
ISOLATION AND CHARACTERIZATION OF THE EXPRESSION OF GENE FOR SUCROSE TRANSPORTER PROTEINS IN SUGARCANE PLANT (SACCHARUM OFFICINARUM) Novita, Harianti; Siswoyo, Tri Agus; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Sucrose as the major transported form of fixed carbon must be translocated from source tissue to the sites of consumption and storage or sink tissues. The translocation of sucrose is facilitated by some distinct sucrose transporters proteins (SUT). To study sucrose transporters in sugarcane, we had conducted isolation and characterization of gene encoding sucrose transporter protein. The isolation was performed with RT-PCR method using total RNA isolated from sugarcane leaf and primer designed from conservative region of SUTcDNAs of SoSUT2A (accession number AY65599), OsSUT-1(accession number AAP54842, OsSUT-1 mRNA (accession number XM 46477). Based on the conservative amino acids sequences of QILQQFA and MGKTEPV, the corresponding sequences of the primers for RT-PCR were: P1, (forward) 5?CAGATCCTTCAACAGTTCGC-3? and P2 (reverse) 5?-TGCCCTTTGTCTCCGGAACC-3?, respectively. Agarose gel electrophoresis shown a clear single 0.5 kb cDNA band of the PCR product. Thus, the DNA was cloned into pGEMT vector (Promega) for further analysis. Sequence determination of the PCR product revealed a nucleotides sequence of 543 bp in length and has a high homology around 89%, 87.3 % and 84.8 % with maize ZmSUT-1, sugarcane SoSUT 2A and rice OsSUT-1 mRNA, respectively. We designated the cDNA as SoSUT2 and the nucleotide sequence have been submitted to GenBank data base under accession number bankit 734628. By using PSORT analysis the fragment of cDNA-SoSUT2 encoded protein may be located in the endoplasmic reticulum. To have a better understanding, the expression of SoSUT2 gene in sugarcane was determinate by RT-PCR method using total RNA isolated from leaf, petioles, stem and root and visualized the PCR product in agarose gel electrophoresis. Based on the cDNA bands intensity, it can be illustrated that the expression of SoSUT2 gene were found highest in sugarcane leafs then petioles and stem, but the expression was not found in root. Although the SoSUT2-cDNA has not been isolated in full size, the results suggest the presence of gene family of SUT in sugarcane.
ANTIOXIDANT ACTIVITY OF POLAR FRACTION OF METHANOL EXTRACT FROM TREE BARK OF SHOREA ACUMINATISSIMA WITH DPPH METHOD Haryoto, Haryoto; Santoso, Broto; Nugroho, Hafid
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Shorea accuminatissima, which is known locally as Meranti kuning, is one species of Dipterocarpaceae family. This plant contains compounds that are expected to have antioxidant activity. This research was carried out to examine the radical scavenger activity of polar fraction of methanolic extract from the tree bark of S. accuminatissima by using DPPH (2,2-difenil- 1-pikrilhidrazil) method with vitamin C as comparator. Powder of tree bark of S. accuminatissima was macerated in methanol. The methanolic extract was then evaporated to obtained dry extract. Extract with concentration of 200, 100, 50, 25 and 10 µg/mL were used to test its radical scavenger activity. The result showed that polar fraction of methanolic extract and vitamin has IC value of 319.83 and 3.72 µg/mL, respectively.
THE USE OF NATA DE COCO MEMBRANE AS BIOSENSOR MATERIAL Mulyono, Tri; Asnawati, Asnawati; Noviandri, Indra; Buchari, Buchari
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Nata de coco has been characterized as filter toward glucose solutions. Standard nata de coco membrane can not be used as membrane because of variations of thickness. It has unequal weight if it was made thinner. Based on its character, nata de coco had been made in various incubation times. Three days incubation time membrane have passed up 76.84 % glucose, 61.15  Lm -2hr -1 flux/permeability. In Low concentration, it?s produced permeate 87% and decrease in increasing concentration. Structures of membrane morphology showed that existence of bovine serum albumin (BSA) in glucose solution possibility didn't influence determination of glucose in blood.
DATA NON-NORMALITY ON AMMI MODELS: BOX-COX TRANSFORMATIONS Hadi, Alfian Futuhul; Sa'diyah, Halimatus; Sumertajaya, I Made
Jurnal ILMU DASAR Vol 8 No 2 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

AMMI (Additive Main Effect Multiplicative Interaction) model for interactions in two-way table provide the major mean for studying stability and adaptability through genotype × environment interaction (GEI), which modeled by full interaction model.  Eligibility of AMMI models depends on that assumption of normally independent distributed error with a constant variance.  In the case of non-normal data distribution, the appropriateness of AMMI model is being doubtful. Transform the observation by power family of Box-Cox transformation is an effort to handle the non-normality. AMMI model then can be applied to the transformed data appropriately following by the use of ordinary least square for estimating parameters.  This approach is investigated by applying them to (i) a count data of pest population of Poisson distribution, which came from a study of leave pest in soybean genotype, and to (ii) a study of rice genotype stability of filled grain per panicle (Binomial data).  One must be carefully considered what the meaning of the transformation in the AMMImodels and Biplot AMMI.

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