cover
Contact Name
Debora Christin Purbani
Contact Email
editor@e-jmsb.id
Phone
+6221-8761356
Journal Mail Official
editor@e-jmsb.id
Editorial Address
Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia
Location
Kota bogor,
Jawa barat
INDONESIA
Journal of Microbial Systematics and Biotechnology
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Articles 14 Documents
NICKEL (NI) REDUCTION IN SOROWAKO POST-MINING SOIL THROUGH APPLICATION OF MYCORRHIZA ACAULOSPORA SP. ASSOCIATED WITH CANAVALIA ENSIFORMIS L. Akib, M. Akhsan; Mustari, Kahar; Kuswinanti, Tutik; Syaiful, Syatrianty Andi; `, Syatrawati; Kumalawati, Z.
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.938 KB) | DOI: 10.37604/jmsb.v1i1.19

Abstract

The nickel (Ni) content in a post-mining soil of Pomalaa mines reached 14,200 mg.kg-1 and became a limiting factor in the plant growth process. A Ni reduction in the soil by using phyto-accumulator such as Jack bean (Canavalia ensiformis L.) can be improved by combining it with arbuscular mycorrhizal (AM) fungi. The purpose of this study was to determine the effect of the mycorrhizal fungus Acaulospora sp. on the efficiency of Ni reduction by C. ensiformis. This experiment was carried out by using a randomized block design with three different treatments, include: 1) C. ensiformis without Acaulospora sp. inoculation (negative control), 2) C. ensiformis inoculated with indigenous Acaulospora sp. and 3) C. ensiformis inoculated with non-indigenous Acaulospora sp. The study was conducted in the nursery that belongs to PT. Vale Indonesia Tbk., Sorowako, South Sulawesi, Indonesia. The results showed that highest nickel accumulation was found in the root inoculated with indigenous Acaulospora sp. (9500 mg.kg-1), followed by stem (1400 mg.kg-1), leaf and pod (1300 mg.kg-1), seed (1200 mg.kg-1), and flower (1100 mg.kg-1). This study indicates that application of the indigenous Acaulospora sp. can improve C. ensiformis efficiency to reduce Ni content at Sorowako post-mining area.
ANTIBIOFILM AND ANTIMICROBIAL ACTIVITIES OF PAPAYA (CARICA PAPAYA L.) AND STEVIA (STEVIA REBAUDIANA BERTONI) LEAF EXTRACTS AGAINST THREE BIOFILM-FORMING BACTERIA Hastuty, Aerma
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.806 KB) | DOI: 10.37604/jmsb.v1i1.18

Abstract

Biofilm is a structural form of a microbial group that is protected by the Extracellular Polymeric Substance (EPS) matrix. The biofilm is considered as the main mediator of infection, and plays a major role in the occurrence of drug resistance. This study was aimed at determining the antimicrobial and antibiofilm activities of papaya (Carica papaya L.) and stevia (Stevia rebaudiana Bertoni) leaf extracts against three biofilm-forming bacteria. The antimicrobial assay showed that papaya leaf extract exhibits higher activity compared to stevia leaf extract in inhibiting the growth of the biofilm-forming bacteria. The optimum condition of papaya leaf extract to inhibit biofilm-forming bacterial growth occurred at 45% and 75% concentrations of the extract (pH 7). A 100% biofilm degradation by papaya leaf extract occurred at pH 6 and pH 9.
THE DECOLORIZATION EFFECT BY ASPERGILLUS SP. 3 ON GOLDFISH OPERCULAR BEATS Dewi, Ratna Stia; Ulfimaturahmah, Fitria Ayudi; Khotimah, Khusnul
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.02 KB) | DOI: 10.37604/jmsb.v1i2.29

Abstract

Batik effluent had high toxicity to aquatic organisms. Dye decolorization is a process used to reduce color density. Fungi which used for decolorization was Aspergillus sp 3. This study aimed to investigate the ability of fungi on decolorization of 3 kinds of batik effluents (Indigosol Green dye, Indigosol Purple dye, and Naphtol Black dye) and to investigate the ability of fungi on reducing TDS level. The study was also investigated the initial toxicity of batik effluents to Goldfish (Cyprinus carpio). Decolorization was measured by spectrophotometry, the pH value was measured by pH meter, and the TDS value was measured by TDS meter. In addition, the decolorized batik effluent was tested for toxic effect on C. carpio by total opercular beats. Decolorization assay showed that Aspergillus sp. 3 had the ability to decolorized only 2 kinds of batik effluents. The decolorization percentage of Indigosol Purple was 60.015% and Naphtol Black was 56.679%. The pH after treatment decreased from 8.5?9 to 5.3?6. The range of pH value of Indigosol Green, Indigosol Purple, and Naphtol Black 5.3?6. Besides that, Aspergillus sp. 3 also had the ability to reduce the TDS level in the effluent. The TDS level on Indigosol Green, Indigosol Purple, and Naphtol Black as 4,965%, 25,307%, and 15,129%, respectively. Initial toxicity assay of effluent to C. carpio showed that there was a difference of total opercular beats, which exposed by decolorized and before decolorized batik effluent. The total opercular beats of C. carpio on decolorized batik effluents showed high value than before decolorized batik effluents. It can be concluded that Aspergillus sp. 3 had the ability to decolorize and decreasing the initial toxicity of Indigosol Purple batik effluents on C. carpio.
COVER JMSB VOL.1 NO.2 (2019) JMSB, Cover
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.111 KB) | DOI: 10.37604/jmsb.v1i2.32

Abstract

OPTIMIZATION OF GROWTH OF OYSTER MUSHROOM MYCELIUM (PLEUROTUS SP.) FROM TASIKMALAYA ON SEVERAL KINDS OF CEREAL MEDIUM Khusnul, Khusnul
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.998 KB) | DOI: 10.37604/jmsb.v1i2.27

Abstract

Pleurotus sp. or oyster mushroom is one type of edible mushroom that contains essential nutrients for the human body. This mushroom has been widely cultivated in Indonesia, one of which is Pleurotus sp. from Tasikmalaya. The key to the success of oyster mushroom cultivation is the seeds and growing medium or baglog, while the factors that influence the quality of the seeds include the type of medium. This study aims to determine 1) the effect of the type of seed medium from the type of cereals used on the growth of mycelium Pleurotus sp. from Tasikmalaya, 2) determine the type of seed medium that has the best mycelium growth. This study uses an experimental method with a Completely Randomized Design (CRD). The parameters measured include the main parameters, namely the rate of growth of mycelium Pleurotus sp. from Tasikmalaya on the seed medium. Supporting parameters are the quality of mycelium growth in the seed medium, the proximate value of cereals used for the seed medium. The results of the analysis showed that the medium of cereals used as seeds ready for cultivation could affect the quality of the seeds produced. The seedling medium from milled corn has the best growth of mycelium.
ANTAGONISTIC ACTIVITY OF THREE ASPERGILLUS ISOLATES AGAINST FUSARIUM WILT OF BANANA Hidayat, Iman; Dewi, Lita Citra; Sukmawati, Dalia
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.165 KB) | DOI: 10.37604/jmsb.v1i1.16

Abstract

Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.
INVENTORY OF MACROFUNGI AT PEAT SWAMP FOREST AREA, KAPUAS HULU, WEST KALIMANTAN `, Noverita; Setia, Tatang Mitra
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.884 KB) | DOI: 10.37604/jmsb.v1i1.17

Abstract

The areas surrounding peat swamp forest at Dusun Meliau and Bukit Peninjau, Kapuas Hulu, West Kalimantan host a diverse species of flora and fauna, including macrofungi. However, the information on the macrofungal diversity in this area has not been reported. The goal of this study was to obtain information on the diversity of macrofungi that can be used as food and medicine. Data was collected by direct modified collection method using plot (10 × 20 m) with interval 10 m along the transect line. A total of 79 specimens of macrofungi discovered from the two locations, of which 23 and 66 specimens were collected from Dusun Meliau and Bukit Peninjau, respectively. Of the 79 macrofungi specimens, 16 specimens were potential as a food source, and 41 as medicine. The macrofungi in this study was dominated by members of Ordo Aphyllophorales.
BIOSORPTION CHROME (CR) AND DYES USING BIOSORBENT IN THE MODIFIED TEA BAG Lestari, Sri; Dewi, Ratna Stia; Wibowo, Eko Setio; `, Atang
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.238 KB) | DOI: 10.37604/jmsb.v1i1.20

Abstract

Biosorption has been known as a potential method in reducing heavy metals and dyes in wastewater, including chrome (Cr) and dyes contents of the wastewater from the batik industry. Straw and spent mushroom compost (SMC) are potential biosorbents due to cheap, abundant, and easily obtained. However, the effectivity of the biosorbents is not only depended upon the type of material but also their surface area. In this study, a modified tea bag was used to increase the surface area of the straw and spent mushroom compost to absorb Cr and dyes from the batik industry wastewater. The optimum of biosorbent ratio and pH in Cr absorption and dyes decolorization was measured. This experiment was conducted using Spilt Plot Design. The results showed that the highest Cr absorption was found at 0,0050 mg/g, and the percentage of decolorization was 68,92% in the biosorbent ratio of 3:1 and pH 5. Biosorbent packed in the tea bags modification was effective removes Cr and dyes in the batik industry wastewater.
THE EFFECT OF SUBSTRATE COMPOSITION ON THE ACTIVITY OF AMYLASE AND CELLULASE BY TRICHODERMA HARZIANUM STRAINS UNDER SOLID STATE FERMENTATION Napitupulu, Toga Pangihotan; Silaban, Nathaniel Reinhart; Kanti, Atit; Sudiana, I Made
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.517 KB) | DOI: 10.37604/jmsb.v1i2.26

Abstract

Trichoderma harzianum is a filamentous fungus that has been known to have biocontrol and plant growth-promoting ability. However, the propagation of this fungus particularly through solid state fermentation (SSF) and characterization of its enzyme activity as one the indicator of quality of fermentation process are still needed to be explored further. Rice grain and its derivative products have economically as well as nutrient composition features beneficial as substrates of fungal propagation through SSF. Therefore, the aim of this study was to investigate the effect of white rice, rice bran, and combination of white rice and rice bran on the activity of amylase and cellulase by Trichoderma harzianum strains under SSF. Two strains of the fungus, InaCC F116 and InaCC F89, as well as their consortium were employed as fungal inoculants. After closed fermentation in dark chamber at 30 ± 1°C for 7 days, the activity of amylolytic as well as cellulolytic enzyme was assayed. The result showed that the presence of rice bran as a substrate increased the activity of crude amylase and crude cellulase. In all substrates, the strain F116 has low activity of both enzymes. The fungal consortium improves the activity of crude enzymes in all substrates. Therefore, the amylase and cellulase activity by T. harzianum in SSF condition were strain- as well as substrate-dependent.
COVER JMSB VOL 1, NO 1 (2019) JMSB, JMSB
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.561 KB) | DOI: 10.37604/jmsb.v1i1.15

Abstract

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