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Jurnal Fitopatologi Indonesia
ISSN : 02157950     EISSN : 23392479     DOI : -
Core Subject : Health,
Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).
Arjuna Subject : -
Articles 240 Documents
FORMULASI BAKTERI ENDOFIT AKAR KEDELAI UNTUK PENGENDALIAN PUSTUL BAKTERI Habazar, Trimurti; Resti, Zurai; Yanti, Yulmira; Sutoyo, Sutoyo; Imelda, Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.147 KB) | DOI: 10.14692/jfi.11.2.51

Abstract

Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
IDENTIFIKASI POLEROVIRUS PENYEBAB KLOROSIS PADA CABAI ASAL BALI, INDONESIA Apindiati, Rita Kurnia; Suastika, Gede; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (790.801 KB) | DOI: 10.14692/jfi.11.2.43

Abstract

Based on the results of a survey conducted on chilipepper plants in Kertha Village, Payangan Subdistrict, Gianyar District, Bali Province, many chilipepper plants with chlorosis symptoms caused by the virus was observed. Infected plants showed yellowing of the lamina, but the veins remain green. Identification was focused on the Pepper vein yellows virus (PeVYV) through transmission using Aphis nasturtii, observation on the morphology of virus particles, total RNA detection by reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequence. The results showed that the disease can be transmitted from infected plant to healthy plant using A. nasturtii. Viral particle has hexagonal shape with the diameter ~30 nm. RT-PCR of total RNA from severe, moderate, and mild symptomatic infected plants, using specific primer sets for coat protein gene of PeVYV successfully amplified DNA with size of ~650 bp. Coat protein gene sequence of PeVYV isolates Bali, Indonesia showing different chlorosis intensity had the highest homology with PeVYV isolates from Taiwan 95.1?96.6%. Based on the data, PeVYV has been confirmed as the cause of chlorosis in chilipepper plants in Bali, Indonesia.
KARAKTERISTIK MORFOLOGI DAN FISIOLOGI ISOLAT PANTOEA STEWARTII SUBSP. STEWARTII PADA JAGUNG Desi, Yulfi; Habazar, Trimurti; Agustian, Agustian; Khairul, Ujang; Syamsuwirman, Syamsuwirman; Novia, Prima
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.074 KB) | DOI: 10.14692/jfi.10.2.45

Abstract

Pantoea stewartii subsp. stewartii is the pathogen causing stewart?s wilt disease in maize. The occurrence of the disease was recently reported in Indonesia with limited information. Research was conducted to characterize several isolates of P. stewartii subsp. stewartii isolated from maize plants collected from 6 districts in West Sumatra. Leaves showing yellow stripes symptoms along the vein was ground and suspeneded then the bacteria was isolated and incubated for 3 × 24 h at room temperature in nutrient glucose agar (NGA). Pure bacterial colonies gained from this medium was used for characterization. Assessment of bacterial characters was conducted based on morphological assay, physiological assay, biological assay (hypersensitivity, and pathogenicity) followed by polymerase chain reaction (PCR)-based identification. Unique characters of the family Enterobacteriaceae was observed on 7 isolates based on morphological and physiological characters, and only 4 isolates (PR1, PP, ST1, LA) gave positive reaction on hypersensitivity and pathogenicity test. Further molecular identification confirmed 3 out of 7 isolates had high homology to Pantoea stewartii, i.e. Pantoea stewartii strain R1-104, R1-132, and ATCC 29923 for isolates LA, PR1, and PR2, respectively.
APLIKASI FORMULA CAMPURAN RIZOBAKTERI UNTUK PENGENDALIAN PENYAKIT BUSUK AKAR RHIZOCTONIA DAN PENINGKATAN HASIL KEDELAI DI TANAH ULTISOL Khaeruni, Andi; Asniah, Asniah; Taufik, Muhammad; Sutariati, Gusti Ayu Kade Sutariati Ayu Kade
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.224 KB) | DOI: 10.14692/jfi.10.2.37

Abstract

Rhizoctonia root rot disease caused by Rhizoctonia solani is one of the most important disease in soybean area, including at ultisol land in Southeast Sulawesi. Rhizobacteria application is one alternative method to control this pathogen. The aim of this experiment was to study of rhizobacteria indigenous formulation to control of Rhizoctonia root rot disease and increase soybean yield in Ultisol soil. A complete randomized design with seven treatments was used in this experiment. The treatments were (A) rhizobacteria formulation for seed treatment, (B) rhizobacteria formulation for seed treatment and repeated at 2 weeks after planting, (C) rhizobacteria formulation for seed treatment, repeated at 2 and 4 weeks after planting, (D) rhizobacteria formulation for seed treatment and fungicide applied at 2 weeks after planting, (E) fungicide seed treatment, and repeated at 2 weeks after planting, (F) fungicide seed treatment, and repeated at 2 and 4 weeks after planting, and control (without rhizobacteria and fungicides). All treatments were inoculated by R. solani and replicated three times. The results showed that rhizobacteria seed treatment and repeated at 2 and 4 weeks after planting was the most effective treatment to control Rhizoctonia root rot disease, and increase plant height and leaf number up to 119% and 170%, respectively, and increased the yield of soybean up to 1870% in ultisol soil compared to plant with control treatment.  
TRICHODERMA SPP. DAN PENICILLIUM SPP. DARI TANAH RIZOSFER LAHAN RAWA LEBAK DALAM MENGINDUKSI KETAHANAN TANAMAN CABAI TERHADAP SERANGAN PENYAKIT REBAH KECAMBAH Muslim, Ahmad; Syahri, Syahri; Hamidson, Harman; Salim, Abdullah
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.422 KB) | DOI: 10.14692/jfi.10.1.31

Abstract

Soil microbes associated with rhizosphere are important for promoting plant growth and inducing resistance to diseases. The research was conducted to study the ability of Trichoderma spp. and Penicillium spp. isolated from rhizosphere in lowland swampy area for controlling damping-off disease caused by Rhizoctonia solani Khun. Trichoderma spp. and Penicillium spp. were cultured in bran, corn meal, and rice straw containing media and applied as inoculum to 2-weeks old seedlings. Application of two fungi isolates effectively induced resistance of chili plants to damping-off disease. Trichoderma spp. and Penicillium spp. were significantly reduced disease incidence by 61.5?100% to 46.2?100%, respectively and disease severity by 50?100% and 30?95.9%, respectively. This experiment showed the potential of Trichoderma spp. and Penicillium spp. as biocontrol agents to control damping-off disease on chili.  
PENAPISAN DAN IDENTIFIKASI BAKTERI AGENS BIOKONTROL PENYAKIT LAYU FUSARIUM HASIL ISOLASI DARI RIZOSFER PISANG Agustiyani, Dwi; Nditasari, Achirul; Laili, Nur; Antonius, Sarjiya
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.425 KB) | DOI: 10.14692/jfi.10.1.23

Abstract

Banana wilt disease caused by Fusarium oxysporum f.sp.cubense is considered as one of the most destructive diseases on banana plants in the tropical region. Biological control agents (BCA?s) have become a promising solution to overcome this disease. The objective of this study was to find potential BCA?s for wilt disease of banana plants. Bacteria and actinomycetes were isolated from banana?s rhizosphere in Lampung and Cianjur. As much as 64 actinomycetes and 142 bacteria isolates were obtained. Antagonistic test against F. oxysporum f. sp. cubense of those isolates showed that 21 bacteria and 10 actinomycete isolates have abilities to inhibit F. oxysporum f. sp. cubense. Actinomycetes showed relatively higher inhibition against F. oxysporum f. sp. cubense compared to bacteria. Isolates which have positive antagonistic activities against F. oxysporum f. sp. cubense were then tested for their protease, chitinase, and selulase activities qualitatively on specific medium. All actinomycetes which were tested had chitinase enzyme activities, while only 5 bacterial isolates had chitinase activities. On the other hand, 13 bacterial isolates showed protease activities and only 1 actinomycete showed protease activity. Two bacterial isolates (L.II.4.ND and L.A.I-5.DW) and 3 actinomycetes (L.A.I.DW, L.3.1.DW and Ci.I.A5.DW) which showed high inhibition against F. oxysporum f. sp. cubense and lyses enzymes activities were identified based on 16S rRNA genes. Analysis based on GenBank data, those isolates have 99% homology to Klebsiella pneumonia (L.II.4.ND), Burkholderia sp. (L.A.I-5.DW), Streptomyces sp. (L.A.I.DW), Streptomyces sp. (L.3.1.DW) and Streptomyces sp. (Ci.I.A5.DW).
SENSITIVITAS METODE SEROLOGI DAN POLYMERASE CHAIN REACTION UNTUK MENDETEKSI BEAN COMMON MOSAIC POTYVIRUS PADA KACANG PANJANG Anggraini, Sherli; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.881 KB) | DOI: 10.14692/jfi.10.1.17

Abstract

Mosaic disease in yard long bean is caused by Bean common mosaic potyvirus (BCMV) and has been reported to affect yield. Common method to detect infection of BCMV involves serological assay and polymerase chain reaction (PCR). The aims of this research is to assess the sensitivity of three methods, i.e. Indirect Enzym-Linked Immunosorbent Assay (I-ELISA), Dot Immunobinding Assay (DIBA), and reverse transcription (RT)-PCR as detection method for BCMV infection in yard long bean. Sensitivity level of the methods was evaluated by diluting plant extract and antisera for I-ELISA and DIBA, and cDNA as template in RT-PCR. Virus isolate from Cirebon was maintained in yard long bean in screenhouse and used for the assessment. Absorbance value of ELISA showed that dilution end point for I-ELISA was reached at 10-3 and 10-2 of plant extract and antisera dilution, respectively. Positive infection was still detected using DIBA when the plant extract was diluted up to 10-5 based on development of color intensity on nitrocellulose membrane. Specific viral DNA fragment was still amplified when cDNA was diluted up to 10-4, indicated higher sensitivity level of RT-PCR method.
PENGELOLAAN PENYAKIT KUNING PADA TANAMAN LADA OLEH PETANI DI WILAYAH BANGKA Munif, Abdul; Sulistiawati, Ita
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.889 KB) | DOI: 10.14692/jfi.10.1.8

Abstract

Yellow disease caused by plant-parasitic nematodes are still a major problem on pepper production area in Bangka region. Significant yield losses occurred due to yellow disease. The objective of this research was to survey the yellow disease incidence and to study farmer?s knowledge, attitude and practices on the management of yellow disease of black pepper in Bangka district, Central Bangka and South Bangka. This study consisted of a survey of the incidence of yellow disease in the field and interviews to the farmers. The result showed that the yellow disease had infected nearly all of black pepper fields in the areas with incidence of as much as 20?60%. Almost all of the respondents reported that the yellow disease was the major disease of black pepper in Bangka region. Farmers? level of knowledge about the causal of disease, symptoms, ecology and control methods of the yellow disease are still very limited. Various control measures have been done by farmers to combat the disease, especially by removing and burning the infected plants or by application of lime. A few farmers did not even controlling the disease. Pesticides have been used by a few farmers to control the yellow disease, but the application of pesticide does not in line with integrated pest management principles.
PENGENDALIAN PENYAKIT BUSUK PANGKAL BATANG KELAPA SAWIT SECARA KULTUR TEKNIS Priwiratama, Hari; Prasetyo, Agus Eko; Susanto, Agus
Jurnal Fitopatologi Indonesia Vol 10 No 1 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.722 KB) | DOI: 10.14692/jfi.10.1.1

Abstract

Effort to manage basal stem rot disease (BSR) caused by Ganoderma boninense could be conducted through several cultural practice approaches. Four series of field experiments was conducted to observe the effectiveness of cultural practice methods for controlling the epidemic of basal stem rot due to G. boninense. Sanitation trial consisted of three treatments i.e root sanitation with big hole planting system, big hole without root sanitation, and standard replanting system as comparison. Hole in hole and standard planting system were compared in the hole in hole trial. Digging and mounding trial was conducted in four treatments, i.e. digging and mounding with teer and Trichoderma, digging and mounding with Trichoderma, mounding with Trichoderma, and infected palm without any treatment as comparison. Meanwhile, a 4 m × 4 m trench was used in the isolation trench trial. Annual observation was conducted to determine the disease incidence of BSR in each trial. Results showed that all methods could prevent palms from G. boninense infection in the early stage of oil palm development. Proper root sanitation delayed G. boninense infection until 2 years after treatment (YAT), hole in hole planting system was able to suppress disease incidence up to 7 YAT, digging and mounding was useful to prolong the life of the infected palms up to 3 YAT, meanwhile the use of isolation trench was demonstrated to prevent G. boninense infection to neighboring palms up to 2 YAT.
DETEKSI DAN IDENTIFIKASI POTYVIRUS PADA UBI JALAR DI TANA TORAJA, SULAWESI SELATAN Anjarsari, Laras; Suastika, Gede; Damayanti, Tri Asmira
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.803 KB) | DOI: 10.14692/jfi.9.6.193

Abstract

Typical viral symptoms including chlorotic and uneven interveinal yellowing on leaves without leaf malformation was observed on sweet potato field in Tana Toraja, South Sulawesi. To identify the causal of the disease, reverse transcription-polymerase chain reaction (RT-PCR) and DNA sequencing were carried out to detect the virus from infected plants. RT-PCR using universal primer for C1 gene of Potyvirus was successfully amplified approximately 683bp DNA fragment. The nucleotide sequences of this C1 gene fragment showed 98% homology to Sweet potato virus G (SPVG). Amplification using specific primer for coat protein (CP) gene of SPVG followed by DNA sequencing confirmed the association of SPVG from infected plants. Further nucleotide analysis shwowed that SPVG isolate from Tana Toraja had 99.2% homology to isolate from Japan. This is the firstt report of SPVG infection on sweet potato in South Sulawesi.

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