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INDONESIA
Jurnal Protobiont
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Core Subject : Education,
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Articles 316 Documents
EFEKTIVITAS ANTIMIKROBA BUAH LAKUM (Cayratia trifolia [L.] DOMIN) TERHADAP PERTUMBUHAN BAKTERI Streptococcus sp. (L.10.3) Yuniar, Helenda Ferni Asri; Rahmawati, Rahmawati; Rousdy, Diah Wulandari
Protobiont Vol 9, No 1 (2020)
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Abstract

Streptococcus sp. (L.10.3) is one of the bacteria that can cause dental plaque. This study was aimed to determine the antimicrobial activity of the fraction of methanol fraction of fruit against Streptococcus sp. (L.10.3). This study used a Completely Randomized Design (CRD), 3 levels of concentrations of fraction methanol fraction 0.3 g/mL, 0.6 g/mL and 0.9 g/mL and 2 positive control treatments of amoxicillin and negative control using distilled water with three times repeated. Testing the antibacterial activity using the Kirby-Bauer method using disc paper. The results of the study showed the average diameter of inhibition zones of Streptococcus sp. (L.10.3) in the 24th hour incubation ranged from 7.70-10.72 mm and in the 48th hour incubation ranged from 8.70-10.37 mm. A concentration of 0.9 g/mL is an effective concentration with a moderate inhibition response category. Giving fraction of methanol fraction of fruit at the lowest concentration of 0.3 g/mL is able to provide a moderate inhibition response with the bacteriocidal antibacterial category.
POLA PERGERAKAN KATAK BATU BERBINTIK HITAM (STAUROIS GUTATTUS GUNTHER, 1859) DI GUNUNG POTENG KALIMANTAN BARAT Jakaria, Mohamad; Junardi, Junardi; Riyandi, Riyandi
Protobiont Vol 9, No 1 (2020)
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Movement patterns has been observed in black-spotted rock frog (Staurois gutattus). The aim of this study was to obtain data movement patterns. This research was conducted at Mountain Poteng, West Kalimantan. Data were analized graphically to explain the relationship between the used parameters. There are 20 individual frogs were observed for 24 hours by using a spool track, which consists of 10 males and females. Chi square test result values of alignment grooves conducted on 20 individual frogs were observed during the 24 hours of observation showed that the ?2 count < ?2 table with a value of (?2 = 0,61 < 3,481, p = 0,05; Chi square test), it shows that the pattern of movement of male and female frogs do not stay away from the starting point of observation.
JENIS BURUNG DI KAMPUS UNIVERSITAS TANJUNGPURA Indra, Sutarto Kusuma; Kustiati, Kustiati; Rafdinal, Rafdinal
Protobiont Vol 9, No 1 (2020)
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Quality degradation, modification, and habitat loss are significant threats to bird species. The natural habitat of birds has been modified into residential land and facilities to meet the needs of human life as happened at Tanjungpura University. This study aims to determine of birds species at Tanjungpura University. Observations were carried out from January to March 2019. The method used in collecting the data from bird was ?Encounter rates? which was conducted in the morning starting at 6 ? 9 am and at 3 - 6 pm. The data obtained were analyzed with the formula of simple abundance scale and frequency of attendance. The birds found at Universitas Tanjungpura are 28 species classified into 23 genera, 17 families, and seven orders. Birds found to have an abundance order scale are classified into abundant, general, frequent and, unusual categories. Birds included in the abundant category are Collocalia fuciphaga and Passer montanus. The types of bird foods at Tanjungpura University consist of frugivore, insectivore, granivore, herbivore, carnivore, piscivore, omnivore, molluscivore, and nectarivore. The value of attendance frequency have range between 10-100%. The bird species with highest frequency of attendance?s value is Passer montanus, Pycnonotus aurigaster, Pycnonotus goiavier, Collocalia fuciphaga, and Anthreptes malacensis.
KARAKTERISASI DAN IDENTIFIKASI GENUS BAKTERI ENDOFIT DARI DAUN DAN BATANG JERUK SIAM (Citrus nobilis var. microcarpa) SEHAT DI DESA ANJUNGAN KALIMANTAN BARAT Silalahi, Lusiana Fitriani; Mukarlina, Mukarlina; Rahmawati, Rahmawati
Protobiont Vol 9, No 1 (2020)
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Endophytic bacteria that live in the tissues of plant organs act as biological control agents and growth accelerator such as for plants Siam Orange (Citrus nobilis var. microcarpa) which is included in main commodity horticultural crops in West Kalimantan. This study was aimed to know the genera of endophytic bacteria from healthy Siam Orange stems and leaves. The study was conducted through the steps of endophyte bacteria isolation, and identification. Systematic sampling method was used and data collected were descriptively analyzed. The study results obtained nine isolates from leaves of Bacillus, Micrococcus, Pseudomonas, Erwinia, Microbacterium, Cellulomonas and one unidentified isolate, one isolate from stems of Micrococcus.
KARAKTERISASI BACILLUS SPP. DAN FUSARIUM SP. DARI TANAMAN LADA (PIPER NIGRUM L.) DI DESA JAGA Flori, Florianus; Mukarlina, Mukarlina; Rahmawati, Rahmawati
Protobiont Vol 9, No 1 (2020)
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Pepper (Piper nigrum L.) is one of the farm crop which become commodities in West Kalimantan. This research aimed to determine the morphological characteristic of Bacillus bacteria from rhizosphere of pepper and Fusarium fungi with symptoms of yellow disease. Sampling was done in pepper farm crops, Sungai Jaga Village, Bengkayang Regency. Isolation and identification were implemented at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak. The result of isolation and identification showed 4 bacterial isolates belong to genus Bacillus, namely Bacillus sp. BRF1, Bacillus sp. BRF2, Bacillus sp. BRF3 and Bacillus sp. BRF4 and one isolate of Fusarium sp. JDF from pepper with symptom of yellow desease.
ETNOBOTANI PAKAIAN ADAT DALAM KEGIATAN RITUAL MASYARAKAT ETNIS DAYAK KANAYATN DI KALIMANTAN BARAT Roberto, Roberto; Rafdinal, Rafdinal; Wardoyo, Elvi Rusmiyanto Pancaning
Protobiont Vol 9, No 1 (2020)
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Dayak Kanayatn as one of the Dayak ethnic in Kalimantan Barat has a tendency to use its natural resource such as plants to support various tradition costum needs, such as tradition clothing which are absolute within the traditional costum ritual. The purpose of this research is to find out the species of plants and its parts that used as material in the Dayak Kanayatn traditional costum clothing. The research was conducted between April until July 2019. The result of the research found that there are 24 species of plants from 12 family. The most used family are the Arecaceae (8 species), Poaceae (5 species), and Moraceae (2 species). Parts of the plants which used the most are branch (44%), then skin of the branch (26%), and the wood (13%). All the plants which are used had its own specific meaning for Dayak Kanayatn people.
PENGARUH KOTORAN AYAM DAN MIKORIZA Glomus aggregatum TERHADAP PERTUMBUHAN KEDELAI (Glycine max) PADA TANAH GAMBUT Iqbal, Muhamad; Linda, Riza; Mukarlina, Mukarlina
Protobiont Vol 9, No 1 (2020)
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Peat soil in West Kalimantan has been contained low of nutrient level so it affects the growth of soybeans. One effort to increase the availability of nutrients in peat soils is by using mycorrhiza and chicken manure fertilizer. This research aims to determine the growth of soybeans by administering chicken manure and mycorrhiza on peat soils. The research was conducted from January to April 2019. The research design used was a Completely Randomized Design Factorial consisting of 2 factors with 4 replications. The first factor is mycorrhiza fungi: M1 (0 gr/polybag), M2 (5 gr/polybag), M3 (10 gr/polybag), M4 (15 gr/polybag) and the second factor is chicken manure fertilizer: P1 (0 tons/ha) , P2 (5 tons/ha), P3 (10 tons/ha), P4 (15 tons/ha). The results showed that the single chicken manure application significantly affected all observed parameters with 15 tons/ha chicken manure fertilizer. Mycorrhiza fungi just a significantly effect on fresh weight and dry weight. Also, the combination of mycorrhiza fungi and chicken manure fertilizer just a significantly affected wet weight and dry weight with combination 5 g mycorrhiza and 5 tons/ha of chicken manure fertilizer.
KEMAMPUAN DEGRADASI SELULOSA OLEH BAKTERI SELULOTIK YANG DIISOLASI DARI TANAH GAMBUT Riza Linda, Anggita Ulfa, Siti Khotimah,
Protobiont Vol 3, No 2 (2014)
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Cellulose is one of organic matters in the peat soil which was difficult to degrade. The degradation can be done enzymatically by cellulotic bacteria. The study was conducted from September 2012 to April 2013. The destination study was to determine genera of cellulotic bacteria which capable degrading the cellulose by the depreciation of crude fiber. The sampling plot was taken of Bansir Darat district in the Southeast Pontianak based on Wollum method. The bacteria were isolated by pour plate method and selected with cellulose hydrolysis method. Identification of bacterial isolates was adapted to Bergey?s manual. Plate count method was used in the enumeration of bacteria, while the level of cellulose degradation was measured using Datta method. Sugar reduction of each genus was measured by Nelson-Somogyi method. Isolation results were obtained 28 isolates, after selected based on selulotic activity index obtained three best isolates (BS20, BS19 and BS17) of 3.2, 2.2 and 2.2. The identification result of three isolates was belonged to the genus Bacillus, Pseudomonas and Nocardia by each of number density was 64.3 x 1012CFU/g, 42 x 1012CFU/g and 42.2 x 1012CFU/g. Cellulose can be degraded was 50%, 8% and 18%. Sugar reduction was produced respectively at 0.017%, 0.043% and 0.019%.
INVENTARISASI JAMUR MAKROSKOPIS DI HUTAN BUKIT DANAU DESA PENIRAMAN KECAMATAN SUNGAI PINYUH KABUPATEN MEMPAWAH Sabrawi, Salmiah; Turnip, Masnur; Ifadatin, Siti
Protobiont Vol 9, No 1 (2020)
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Macroscopic mushrooms are one of the important biodiversity in the ecosystem of forests and human life. Research related to macroscopic mushrooms in West Kalimantan has already been done, but have never done in some areas, which is in the forest of Bukit Danau Peniraman Village of Mempawah regency. This research aims to determine the types of macroscopic mushrooms that exist in the forest of Bukit Danau Peniraman Village of Sungai Pinyuh district, Mempawah regency. Sampling of fungi using exploration methods by exploring the forest according to the transect pathway. The results showed that there were 20 types of macroscopic mushrooms consisting of 5 orders, 13 families, and 17 generas. Fungi that most commonly found came from an order of Polyporales and the fewest orders are the order ofDacrymycetales, the order of Hymenochaetales, and the order of Xylariales. Macroscopic mushrooms found in the forest of Bukit Danau Peniraman Village of Sungai Pinyuh district, Mempawah regency, fungi most commonly grew at substrate of dead tree.
PERTUMBUHAN KEDELAI (Glycine max [L.] Merrill) VARIETAS ANJASMORO DENGAN PEMBERIAN BIOURIN KAMBING (Capra aegagrus hircus) Hamid, Abd.; Linda, Riza; Mukarlina, Mukarlina
Protobiont Vol 9, No 1 (2020)
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Soybean (Glycine max [L.] Merrill) Anjasmoro variety is one of the superior soybean varieties widely cultivated in West Kalimantan. The growth of soybean can be increased by applying liquid organic fertilizer namely goat biourine. The purpose of this study was to determine the goat biourine quality and the effect of the application of goat biourine on the growth of Anjasmoro variety soybean plants. This study uses a Completely Randomized Design (CRD) consisting of five treatments, namely without biourine/control, 100 mL/L biourine, 200 mL/L biourine, 300 biourine mL/L and  400 mL/L biourine. Each treatment was repeated 5 times to obtain 25 experimental units. The analysis of goat biourine showed the value of C-Organic 2.03%, pH 4.83, N 0.72%, P 0.004% and K 0.234%. The results showed that apply of goat biourine had a significant effect on plant height, number of leaves, shoot wet of weight, shoot dry of weight, number of branches, number of root nodules and the number of effective root nodules, but it had not a significant effect on root wet of weight and root dry of weight.

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