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Jurnal Sain Veteriner
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Articles 511 Documents
Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani Budipitojo, Teguh; -, Ariana; Pangestiningsih, Tri Wahyu; Wijayanto, Hery; Kusindarta, Dwi Liliek; Musana, Dewi Kania
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5968.722 KB) | DOI: 10.22146/jsv.34698

Abstract

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of  Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic  documentation of  Indonesia’s native biodiversity. 
Pengaruh Ekstrak Buah Delima Terstandar 40% Ellagic Acid terhadap Profil Darah Tikus Putih Yang Mengalami Nefrotoksisitas akibat Induksi Gentamisin Lukiswanto, Bambang Sektiari; Yuniarti, Wiwik Misaco
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7994.355 KB) | DOI: 10.22146/jsv.34697

Abstract

The purpose of this study was to determine the effect of pomegranate extract of 40% ellagic acid on blood profile of albino rats that had nephrotoxicity after included with Gentamicin. a total of 32 white rats were divided into four treatment groups, each consisting of eight rats. the four treatment groups were healthy rats (P0), Gentamicin induced rats (P1), Gentamicin induced rats and ellagic acid treatment (P2) and Gentamicin induced rats and standardized Pomegranate extract therapy 40% ellagic acid treatment (P3). After seven days of treatment, blood was taken for blood profile tests. The results showed that in the Gentamicin-induced group (P0), a decrease in blood profile allegedly caused by Gentamicin that could cause damage and impaired renal tubular function. Givibg ellagic acid and pomegranate extracts of 40% ellagic acid proved to improve the blood profile of Gentamicin induced rats. This is due to inhibition of renal damage and improved renal function due to antioxidant activity possessed by ellagic acid and pomegranate extract of standardized 40% ellagic acid.
Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan Gustina, Sri; Hasbi, Hasbi; Kurniani Karja, Ni Wayan; Setiadi, Mohamad Agus
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7327.448 KB) | DOI: 10.22146/jsv.34695

Abstract

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries. 
Immunocytochemical Study on Blood and Organ Suspension of Tiger Grouper (Epinephelus fuscoguttatus) Infected with Field Isolate of Viral Nervous Necrosis Lestari, Artanti Tri; Sudaryatma, Putu Eka
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1097.367 KB) | DOI: 10.22146/jsv.5426

Abstract

One potential marine cultures that have been developed and started to show the international market is grouper. Grouper culture can not be separated from factors that can affect disease and thwart cultivation. One of the diseases that has been reported by researchers is viral nervous necrosis (VNN) causing mass mortality in fish, especially grouper larvae and juvenile stadia. Laboratory of Balai KIPM kelas l Denpasar develop rapid diagnostic techniques, precise and accurate test using immunocytochemistry of blood and organs as one of the initial inspection. Tiger grouper sized 150-300 g as much as 50 and acclimatized, then 10 fishes used as controls, 40 fishes were injected with inoculum VNN 101.5 reared without water replacement cycle for ten days. Clinical observation and organ sampling performed 12 hours post-infection and consecutive every 12 hours. Blood samples and organs were collected for immunocytochemical (streptavidin-biotin) and a confirmatory test using RT - PCR using kit IQ -2000 VNN. Immunocytochemistry and RT-PCR showed positive results against VNN blood smears and suspensions organs of grouper fish with 24 hours post-infection . Based on the test results, the immunocytochemistry test on the blood and organ suspensions can be used as a detection technique VNN which is rapid, precise and accurate. 
Prevalence of Toxoplasmosis in Sheep Slaughtered in Ngampilan Slaughterhouse Yogyakarta Using CATT Method Siregar, Rika Yuniar; ., Yuswandi
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1135.786 KB) | DOI: 10.22146/jsv.5425

Abstract

The research was conducted to find out the prevalence of toxoplasmosis in sheeps which were slaughtered in Ngampilan Slaughterhouse Yogyakarta using card agglutination test (CATT) method. Blood samples were collected from 50 sheeps. The blood samples were then centrifuged at 5000 rpm for 5 minutes in 4°C in order to obtain the sera of the sheeps. After that, each serum in the amounts of 10 µl was tested using CATT method. The positive CATT test resulted in the green background with red aggregate in the middle, whereas the negative CATT test resulted in the uniform brown discoloration. In conclusion, about 72% of sheeps slaughtered in Ngampilan Slaughterhouse Yogyakarta was positively infected toxoplasma. Toxoplasmosis prevalences for ewes, rams, sheeps and lambs are 79 %, 59 %, 72 % and 67 %, respectively. 
Total Plate Count of Milk from Dairy Cooperatives in Yogyakarta and East Java Septiani, Monica; Drastini, Yatri
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.398 KB) | DOI: 10.22146/jsv.5424

Abstract

Most of the local populations in Indonesia, especially in Sleman, Yogyakarta and Tulungagung, East Java consume cow milk. Cow milk is more widely consumed than that of horse or goat because of the people  habit and the raw milk is more easily found in the market. The majority of people consume milk without knowing the number of bacteria contained and neither the presence nor absence of harmful bacteria in the milk consumed. This study aimed to identify the total number of bacteria in several dairy cooperatives in Sleman, Yogyakarta and Tulungagung, East Java. Milk samples were taken from two tanks in Koperasi Sarono Makmur (KSM), three tanks (were taken four times, once a week) in Koperasi Warga Mulya (KWM) Sleman, Yogyakarta, and two tanks (twice in the morning and once in the evening) in Koperasi Unit Tani Ternak (KUTT) Tulungagung, East Java. Milk from each tank were taken 500 mL and put into the cooler that was given ice cubes. Then the samples to be tested for total bacteria from each tank with plate count agar medium. The number of samples was 19. Milk samples were statistically analyzed with one way ANOVA. The results showed that the total number of bacteria of milk of KSM is 1.0 x 106 CFU/mL, KWM 3.6 x 106 CFU/mL, whereas cow's milk KUTT 4.0 x 106 CFU/mL. The average total number of bacteria of KWM and KUTT exceeds the requirements of the Indonesian National Standard (SNI) 01-3141-1998 (1,0 x 106 CFU/mL).
The Influence of Kebar Grass Infuse to Mice (Mus musculus) Spermatogenesis Lefaan, Paula Nency
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1097.132 KB) | DOI: 10.22146/jsv.5423

Abstract

This study was aim to examine the effect of grass infusion kebar (Biophytum petersianum) to increase the activity of spermatogenesis in male mice. This research was conducted at the Laboratory of Botany University Discourse Kristen Duta Yogyakarta, Laboratory of Experimental Animal Care Unit and Unit III LPPT Gadjah Mada University in April 2009 to May 2009 using Swiss strain male mice aged 2.5 months as many as 25 tails, which are grouped in 5 groups treated with different concentrations, 0% (control), 1%, 3%, 5%, and 10% for 30 days, 2 times a day at a dose of 0.5 ml each time. Results of TLC method proves that these plants contain three types of chemical compounds that could potentially impact the process of spermatogenesis, ie, saponins, flavonoids and tannins. The analysis showed an increase in activity with increasing concentrations of spermatogenesis up to a concentration of 5% (spermatogonia: 226, primary spermatocytes: 227, secondary spermatocytes: 377, spermatids: 302) with a significant value of 0.432. Yet, at a concentration of 10% actually decreased spermatogenesis activity due to negative feedback. Conclusion: Infuse kebar grass proved to increase the activity of spermatogenesis, but at concentrations that are too high can cause a decrease in spermatogenesis activity.
Review of The Artificial Insemination Result Based on Anestrus Post Insemination in Bali Cattle Herds at The Regency of Sikka, East Nusa Tenggara Topianong, Tarsisius Considus; Budiyanto, Agung; Setyawan, Erif Maha Nugraha
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.101 KB) | DOI: 10.22146/jsv.5422

Abstract

Anestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood,  the cattle with pregnancy condition will not show the signs of estrus.  The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of  the role of inseminator on the success of AI and  then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %.  Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the  farmers who did not have any ability or authority to perform  medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy  followed by the process of parturition.
The Effect of Various Thawing Temperatures of Frozen Semen on the Success of Artificial Insemination of Beef Cattle Wulandari, Ida Arlita; Prihatno, Surya Agus
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.688 KB) | DOI: 10.22146/jsv.5421

Abstract

The study about the effect of various thawing temperatures frozen semen on the success of artificial insemination of beef cattle had been conducted in Residency of Sleman Territory of Yogyakarta. The study used 59 heifers and cows that were oestrus (without oestrus stimulation) with body condition score (BCS) of 3 and 1,5-8 years age. They were inseminated using frozen semen of Simmental thawed in temperature of 37°C, 35°C (warm water) and 28-30°C (cold water). Effect of thawing temperatures was evaluated by non returnrate (NR). NR of thawing temperatures of 37°C, 35°C and 28-30°C were 63,16%, 55%, and 45%. Thawing temperature of 37°C increased the non returnrate compared to the thawing temperatures of 35°C and 28-30°C. Statistical analysis by Chi-square showed there were no differences between thawing temperatures of  37°C, 35°C and 28-30°C on the success of artificial insemination
The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls Utami, Tri; Topianong, Tarsisius Considus
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.301 KB) | DOI: 10.22146/jsv.5420

Abstract

The percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were used 10 straws of FH bulls in the form of 0.25 ml. A total of 10 straws then divided into two treatment groups of  thawing  in water at 37°C and water 8°C, respectively. An examination of the motility and morphology of spermatozoa abnormalities performed every two hours for two times. Calculating the percentage of sperm motility was done by calculating the percentage of spermatozoa moving forward in the field of view under a microscope with a magnification of 10x. the percentage of abnormal spermatozoa was  assessed by William's stain. Spermatozoa morphology was observed by using a microscope magnification of 100x. Abnormalities of spermatozoa were calculated from a total of 200 spermatozoa, either normal or abnormal. At the same thawing time, the motility of FH cattle sperms post thawing in water temperature 37°C had a higher preference than that of post thawing in water temperature 8°C, although it was not significantly different (P > 0.05).  Based on morphological aspects, frozen semen used in this study is within the tolerance limit for the total percentage of abnormal sperm morphology between 12% to 23% and normal morphology between 70% to 88%. 

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