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Jurnal Kedokteran Hewan
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Articles 345 Documents
THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION Sari, Eka Meutia; Tanjung, Satria; Sari, Dewi Ratna; Akmal, Muslim; Siregar, Tongku Nizwan; Thasmi, Cut Nila
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.14821

Abstract

This study aimed to determine the effect of prostaglandin F2? (PGF2?) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2? administration at a dose of 25 mg and PGF2? at a dose of 37.5 mg had no effect (P 0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.5±0.9; 6.3±0.6; and 6.2±1.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.7±0.6; 7.1±0.2; and 6.5±0.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,328±96.43 x 106 cells/mL; 1,354±102.19 x 106 cells/mL; and 1,353.3±88.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.3±2.8%; 71.6±2.8%; and 73.3±2.8%, respectively. Testosterone levels in P1 and P3 were 5.05±0.22 and 6.74±1.38 ng/mL, respectively (P 0.05). Based on the results of this study, it was concluded that the administration of PGF2? does not affect semen quality but can increase the level of testosterone on Bali cattle.
POTENTIAL OF ACEH ARABICA COFFEE EXTRACT (COFFEA ARABICA L.) IN REJUVENATION OF AGING SKIN IN RAT Safrida, Safrida; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.8050

Abstract

This study aimed to u determine the potency of Aceh Arabica coffee In rejuvenation of aging skin in rat. This study was conducted in vivousing completely randomized design (RAL), which consisted of 4 treatment groups as follows: premenopausal rats as negative control (KON),premenopausal rats with administration of distilled water as placebo (PLS), premenopausal rats with administration of ethinylestradiol 9x10-3mg/day/200 g BW (EST), and premenopausal rats with coffee extract administration of 300 mg/day/200 g BW (KOP). Each treatment groupconsisted of 5 rats. The parameters observed were skin collagen, water content/skin hydration, and skin ribonucleic acid (RNA) level. The datawere analyzed using analysis of variance (ANOVA) and Duncan test using SAS 9.13 software. The result showed the average skin collagen andRNA levels were higher in premenopausal rats with administration of coffee extract and ethinylestradiol (P 0.05) than control rats. On the otherhand, average of water level in premenopausal rats with coffee extract and ethinylestradiol administration were the same as control rats. It can beconcluded that administration of Aceh Arabica coffee extract can repair skin quality, which is indicated by the increase of collagen and RNAlevel on aging skin in rat.
IDENTIFIKASI DAN KARAKTERISASI FENOTIPE STAPHYLOCOCCUS AUREUS ASAL KASUS BUMBLEFOOT DAN ARTHRITIS PADA BROILER k, Khusnan; Prihtiyantoro, Wahyu; Slipranata, Mitra
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.332

Abstract

Penelitian ini bertujuan mengidentifikasi dan karakterisasi Staphylococcus aureus (S. aureus) yang berasal dari kasus bumblefoot dan arthritis. Dalam penelitian ini digunakan 10 isolat S. aureus yang terdiri atas lima hasil isolasi asal kasus bumblefoot dan lima hasil isolasi asal kasus arthritis pada ayam broiler. Identifikasi S. aureus dilakukan melalui uji fermentasi manitol salt agar (MSA), pewarnaan Gram, uji koagulase, clumping factor, dan uji katalase. Karakterisasi fenotipe S. aureus yang dilakukan meliputi sifat hemolisis pada plat agar darah, sifat hidrofobisitas, dan kemampuan hemaglutinasi. Hasil penelitian menunjukkan bahwa semua isolat S. aureus mampu memfermentasi MSA, positif uji koagulase, clumping factor, dan katalase. Staphylococcus aureus mampu menghemolisis plat agar darah dengan memperlihatkan sifat ?-hemolisin (80%), ?-hemolisin (10%), and ?-hemolisin (10%). Semua isolat S. aureus (100%) bersifat hidrofil dan mampu mengaglutinasi sel darah merah kelinci.
THE CONTRACEPTIVE EFFECT OF THE EXTRACT METHANOL OF THE LEAVES HYDROCOTYLE JAVANICA THUMB ON FEMALE MICE R, Rinidar; Isa, M.
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i1.3117

Abstract

The aim of this study is to investigate the contraceptive effect of the extract methanol of the leavesHydrocotyle javanica Thumb and its effect threat of implantation, the quantity and condition of fetuses,histology appearance of the ovary and uterus and rate of pregnancy. The female mice were given orallyeither methanol extract of leaves, for 10 days in various doses: 20, 40, 60, and 80 gram/kg BW. On day20, the mice were mated and when pregnancy occurred, treatment was stopped. On day 17 of pregnancy,the mice were sacrificed: uterus and ovaries were examined histologically. In conclusion, methanol extractof Hydrocotyle javanica Thumb shows contraceptive activity (P 0.05).Keywords: extract methanol, Hydrocotyle javanica Thumb, contraceptive
GAMBARAN HISTOLOGIS TUBULUS SEMINIFERUS KAMBING KACANG JANTAN LOKAL PASCAPEMBERIAN SUPLEMEN DAUN KATUK Ferasyi, Teuku Reza; Budiman, Hamdani; Akmal, Muslim; Melia, Juli; R, Razali; Novita, Andi; Barus, Rina Aulia; Suprayogi, Agik
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1261

Abstract

Penelitian ini mengetahui pengaruh pemberian tepung atau ekstrak alkohol daun katuk terhadap perubahan histologis testis kambing kacang lokal jantan. Dalam penelitian ini digunakan 15 ekor kambing dengan umur sekitar 1,5 tahun. Semua hewan penelitian terlebih dahulu diaklimatisasi selama 2 minggu terhadap lingkungan penelitian. Hewan-hewan tersebut dibagi menjadi 3 kelompok yang masing-masing terdiri atas 5 ekor kambing. Kelompok P0 (kontrol) telah hanya diberikan air distilasi. Pada kelompok P1, diberikan perlakuan berupa suplementasi tepung daun katuk dan untuk kelompok P2 diberikan ekstrak alkohol daun katuk. Semua perlakuan diberikan secara oral sebanyak dua kali per hari (pada pagi dan sore hari) selama 35 hari berturut-turut. Setiap kambing percobaan diberikan pakan yang terdiri atas daun-daunan dan rumput, serta disediakan akses untuk memperoleh air minum secara ad libitum. Pada akhir waktu penelitian, seluruh hewan dikastrasi dan bagian tubulus seminiferus dari testis diambil dan diproses lebih lanjut untuk evaluasi secara histologis. Hasil yang diperoleh menunjukkan bahwa terdapat indikasi peningkatan produksi spermatid dan spermatosit pada Kelompok P1 dan P2. Dengan demikian disimpulkan bahwa pemberian suplemen daun katuk dapat meningkatkan produksi spermatid dan spermatosit kambing kacang lokal jantan.
DEVELOPMENT OF THE CORTICAL AND MEDULLARY CELLS OF ADRENAL GLAND IN THE LONG-TAILED MACAQUE (MACACA FASCICULARIS) DURING PRE- AND POSTNATAL PERIOD Cahyadi, Danang Dwi; Nurhidayat, Nurhidayat; Supratikno, Supratikno; Novelina, Savitri; Nisa', Chairun; Setijanto, Heru
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.12044

Abstract

The study aimed to analyze the dynamics of the development of cortical and medullary cells of adrenal gland in the long-tailed macaque (Macaca fascicularis) during pre- and postnatal period. The samples of adrenal glands were taken from 5 fetuses aged 70, 85, 100, 120, and 150 days of gestation, a newborn aged 10, and an infant aged 105 days old. The samples of the adrenal glands were stained with hematoxylin-eosin and Masson trichrome (Goldner?s modification). The results showed that the fetal adrenal cortex consisted of a definitive and fetal zones, but in the late gestation (fetus aged 150 days of gestation), the transitional zone appeared between both zones. At the postnatal period, the definitive zone changed to glomerulosa zone, while the outer cells of the transitional zone changed to fasciculata zone which radially arranged. The results indicated that the fetal zone was present until birth and it will disappear gradually after birth. The medullary cells were found at the fetal stages as an irregular structure of small islands of chromaffin cells in the fetal zone. This structure migrated slowly to the center of the adrenal glands. The characteristics of mature chromaffin cells were found in the adrenal glands at postnatal period. In conclusion, the cortex adrenal of the newborn of long-tailed macaque consisted of zona glomerulosa, zona fasciculata, zona transitional, and the remaining fetal zone that has dissapeared gradually after birth.
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P 0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (AZADIRACHTA INDICA) ON TRYPANOSOMA EVANSI IN RATS (RATTUS NORVEGICUS) Fahrimal, Yudha; Maghfirah, Siti; Rinidar, Rinidar; Azhar, Al; Asmilia, Nuzul; Erina, Erina
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5450

Abstract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.
BRIGHTNESS-MODE EKOKARDIOGRAFI DOMBA JAWA JANTAN EKOR TIPIS Fakhrul Ulum, Mokhamad; Utami, Nur Fitri; Dwi Utami, Nindya; Noviana, Deni
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2838

Abstract

Penelitian ini bertujuan mencitrakan jantung domba jawa ekor tipis (DET) secara ekokardiografi. Domba ekor tipis jantan sebanyak lima ekor dengan berat 14-16 kg berumur 10-12 bulan digunakan dalam penelitian ini. Domba dipegang dan dibaringkan pada meja khusus tanpa sedasi ataupun pembiusan. Organ jantung domba dicitrakan menggunakan ultrasonografi brightness-mode (B-mode). Transduser cembung dengan frekuensi 2,5-4 MHz digunakan untuk mencitrakan jantung pada posisi right parasternal (RPS) view dan left parasternal (LPS) viewdengan posisi long axis (LAx) dan short axis (SAx). Hasil pencitraan ekokardiografi menunjukkan bahwa bagian-bagian dari organ jantung yang tersusun atas cairan terkesan anechoic, sedangkan bagian yang tersusun atas jaringan lunak terkesan hypoechoic dengan derajat ekogenisitas yang bervariasi. Bagian-bagian struktur jantung dapat dibedakan menurut ruang jantung atrium kanan, ventrikel kanan, atrium kiri dan ventrikel kiri. Bagian-bagian jantung yang dapat dicitrakan seperti septa jantung, otot papilaris, katup jantung semilunaris, katup jantung mitralis, katup jantung trikuspidalis, pembuluh darah vena paru-paru dan aorta. Ekokardiografi B-mode pada organ jantung domba jawa ekor tipis dapat mencitrakan dengan baik struktur ruang, otot, katup dan pembuluh darah besar dengan derajat ekogenisitas yang bervariasi.
EFFECT OF PMSG ADMINISTRATION IN COMBINATION WITH VAGINAL SPONGE ON ESTROUS OCCURRENCE AND LITTER SIZE OF JAVANESE SHEEP Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100%  of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively.  Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep

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