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Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin Jusri, Ridza; Widodo, Wahyu Setia; Widowati, Wahyu; Armansyah, Adek; Sormin, Delores Elisabeth; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1629

Abstract

Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of Pineapple Core Extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H2O2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H2O2 scavenging activity of around 24.12±3.13 μg/ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCEPerbandingan Aktivitas Antioksidan dan Antihialuronidase Ekstrak Bonggol Buah Nanas (EBN) dengan Senyawa LuteolinTerdapat dua jenis proses penuaan kulit, penuaan kronologis dan penuaan dini. Penuaan dini umumnya disebabkan oleh radikal bebas, baik dari polusi udara maupun photoaging. Penggunaan antioksidan dan antihialuronidase untuk mencegah proses penuaan telah banyak diketahui. Ananascomosus merupakan salah satu tanaman yang memiliki potensi sebagai antipenuaan dan antioksidan karena mengandung sejumlah senyawa fenolik yang memiliki berbagai aktivitas biologis. Salah satu senyawa fenolik utama dalam A. comosus adalah luteolin. Tujuan penelitian ini adalah untuk mengevaluasi potensi antioksidan dan anti-aging dari Ekstrak Bonggol Nanas (EBN) dan senyawa pembandingnya yaitu luteolin . Penelitian ini dilakukan di Biomolecular and Biomedicine Research Center, Aretha Medika Utama dari Agustus hingga November 2018. Pengujian antioksidan dari EBN dan luteolin  dilakukan menggunakan uji pemerangkapan H2O2. Uji antipenuaan dilakukan melalui uji penghambatan aktivitas hialuronidase, salah satu enzim penting dalam proses penuaan. Hasil penelitian menunjukkan bahwa Luteolin  memiliki nilai IC50 aktivitas pemerangkapan H2O2lebih rendah sekitar 24.12±3.13 μg/ml lebih baik daripada EBN dengan IC50 sebesar 304.56±3.76μg/mL. Hasil uji aktivitas penghambatan hialuronidase, senyawa luteolin  memiliki nilai IC50 lebih rendah, yaitu sebesar 67,38±3,99 μg/mL dibanding dengan EBN nilai IC50 161,15±1,05μg/mL. Hasil ini menunjukkan bahwa senyawa pembanding luteolin  memiliki aktivitas antioksidan dan antihialuronidase yang lebih tinggi daripada EBN. 
Rituximab Iodination Procedure for Radioiodinated Rituximab (131I-Rituximab) Preparation Ramli, Martalena; Hidayat, Basuki; Sutari, Sutari; Setyowati, Sri; Susilo, Veronica Yulianti
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1864.956 KB) | DOI: 10.15395/mkb.v51n2.1595

Abstract

Rituximab is a chimeric monoclonal antibody which has specific for CD20 antigen expressed by pre-B and mature B-cells. Radiolabelled Rituximab, 131I-Rituximab, has been sucessfully used for treatment of B-Cell NHL. Due to its short shelf-life, 131I-Rituximab is commonly freshly prepared in hospitals prior to its used.  This study aimed to validate rituximab iodination procedure for 131I-Rituximab preparation in order to find the most suitable procedure to be applied in hospitals which intend to produce 131I-Rituximab in-house.  Three different methods of radiolabelling using three types of oxidizing agents, namely Iodobeads, Iodogen, and Chloramine-T were performed. Prior to the validation, radiochemical purity test and purification procedures were also validated as these procedures are critical for producing an acceptable quality of  I-Rituximab. In addition, the shelf-life of 131I-Rituximab was also studied. This study was conducted at the Centre for Radioisotope and Radiopharmaceutical Technology, Serpong during the period of July 2015 to February 2018.  The results showed that the radiochemical purity test of 131I-Rituximab could be easily performed by using instance thin layer chromatography–silica gel (ITLC-SG) in the stationary phase and 85% methanol or saline in the mobile phase. Purification of 131I-Rituximab was conducted using a Sephadex G-25 M filled column with 0.1 M PBS, pH 7.2, as the eluent that was found to be quite reliable to give 131I-Rituximab with radiochemical purity of >95% and recovery of approximately 90%. Radiolabelling efficiency performed using Iodobeads was the lowest (60%) compared to that of Iodogen and Chloramine-T (80–90%). In addition, approximately 30% of  I was retained by Iodobeads and this procedure was time consuming(~ 1 hours). It is concluded that Chloramine-T and Iodogen are better than Iodobeads as the oxidizing agent for radiolabelling of Rituximab with 131I. The radiochemical purity of 131I-Rituximab is well maintained when stored at room temperature and in 4 °C temperature up to 6 hours.Validasi Prosedur Iodinasi Rituximab untuk Preparasi131 I-RituximabValidasi proseduri odinasi rituximab untuk preparasi131I-Rituximab telah berhasil dilakukan.  Validasi ini dilakukan untuk mendapatkan prosedur yang paling sesuai yang dapat diaplikasikan untuk produksi131 I-Rituximabdi rumah sakit yang ingin memproduksi131 I-Rituximab di laboratorium mereka.  Tiga metode radiolabelling menggunakan 3 jenis oksidator Iodobeads, Iodogen, dan Chloramine-T telah divalidasi. Sebelum validasi ini, prosedur uji kemurnian radiokimia dan pemurnian divalidasi terlebih dahulu karena prosedur-prosedur ini sangat berpengaruh dalam penyediaan131 I-Rituximab dengan kualitas yang baik. Disampingitu, lama simpan131I-Rituximab juga dipelajari. Penelitan ini dilaksanakan di Pusat Teknologi Radioisotop dan Radiofarmaka, Serpong, Juli 2015–Februari 2018. Hasil penelitian memperlihatkan bahwa uji kemurnian radiokimia 131I-Rituximab dapat dilakukan dengan mudah menggunakan instance thin layer chromatography – silica gel (ITLC-SG) sebagai fasa diam dan metanol 85% atau larutan salin sebagai fasa gerak. Pemurnian131I-Rituximab menggunakan kolom Sephadex G-25 M dan0.1 M PBS pH 7,2 sebagai eluen dapat diandalkan dan memberikan131I-Rituximab dengan kemurnian radiokimia >95% dan sekitar 90% perolehan kembali. Efisiensi penandaaan menggunakan Iodobeads didapatkan paling (60%) dibanding dengan Iodogen dan Chloramine-T (80 – 90%). Di samping itu, sekitar 30% 131I hilang karena terikat pada Iodobeads dan prosedur ini memakan waktu yang panjang (~1 jam). Penandaan Rituximab 131I menggunakan Chloramine-T and Iodogen dapat disimpulkan lebih baik dibanding dengan menggunakan Iodobeads. Kemurnian radiokimia131I-Rituximab terjaga dengan baik pada penyimpanan selama 6 jam pada suhu kamar dan 4 °C.
PEDIATRIC HAND BLAST INJURY PROFILE IN EMERGENCY ROOM OF DR. HASAN SADIKIN GENERAL HOSPITAL BANDUNG Ismiarto, Yoyos Dias; Arif, Yoan Putrasos
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.006 KB) | DOI: 10.15395/mkb.v51n2.1643

Abstract

In the past, blast injuries were mainly caused by war-related incidents. However, despite the current lack of war-related activities, blast injuries are still seen due to unintentional low explosive material blasts which are mostly seen on children?s hand. However, the classification for the severity of this type of injury is still unclear. The aim of this study was to assess the incident, patterns, and severity of hand blast injuries among pediatric patients treated in Dr.Hasan Sadikin General Hospital. Twenty two pediatric patients in Dr. Hasan Sadikin Hospital Bandung from 2013?2017 were included in this study. All data were extracted retrospectively from patient medical records and the degree of injury severity was assessed using Hand Injury Severity Score (HISS). There were 22 hand injuries caused by explosion identified in 18 boys (82%) with the age range of 5 to 15 years. Hand injuries from explosions have a specific pattern with the hand radial side experiencing the most severe damage including the thumb (16 cases), index finger (16 cases), and middle finger (14 cases). The severity of the injuries varied from mild (3 cases) to moderate (4 cases), severe (7 cases), and major (8 cases).  A high incidence of major hand blast injuries is found among pediatric patients with the hand radial side tends to suffer the most severe damage.Profil Cedera Tangan Akibat Ledakan pada Anak di Ruangan Gawat Darurat RSUP Hasan Sadikin BandungCedera akibat ledakan di masa lalu terutama disebabkan oleh situasi peperangan. Saat ini cedera akibat ledakan masih terjadi terutama pada tangan anak yang disebabkan oleh ledakan bahan berdaya rendah yang tidak disengaja. Namun, klasifikasi untuk menentukan derajat keparahan cedera masih belum jelas. Tujuan dari penelitian ini adalah menganalisis insidensi, pola, dan derajat keparahan cedera terhadap pasien yang dirawat di Rumah Sakit Hasan Sadikin. Penelitian ini melibatkan 22 pasien anak di Rumah Sakit Dr. Hasan Sadikin Bandung periode 2013-2017. Semua data didapatkan secara retrospektif dari rekam medis pasien. Tingkat keparahan cedera dinilai menggunakan Hand Severity Severity Score (HISS). Dua puluh dua cedera tangan akibat ledakan teridentifikasi. Kelompok ini sebagian besar terdiri atas 18 anak laki-laki dengan rentang usia 5 hingga 15 tahun. Cedera tangan akibat ledakan memiliki pola tertentu, dengan sisi radial tangan mengalami kerusakan paling parah di antaranya 15 kasus mengenai ibu jari, 16 kasus mengenai jari telunjuk, dan 14 kasus mengenai jari tengah. Tingkat keparahan cedera bervariasi dari kasus ringan sebanyak 3 kasus, sedang sebanyak 4 kasus, berat sebanyak 7 kasus, dan parah sebanyak 8 kasus. Cedera ledakan di tangan anak memiliki angka kejadian yang tinggi dalam kategori berat dengan sisi radial tangan cenderung menderita kerusakan paling parah.
CRANIAL CHARACTERISTICS, MAXILLOFACIAL, AND SKULL BASE STRUCTURE OF NON-HUMAN PRIMATE (ADULT MACACA FASCICULARIS): A PRELIMINARY STUDY FOR CRANIAL CRANIOTOMY MODEL Bolly, Hendrikus Masang Ban; Indah, Astrina Rosaria; Faried, Ahmad; Noverina, Rachmawati; Arifin, Muhammad Zafrullah; Wirakusumah, Firman Fuad
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1650

Abstract

The use of non-human primate (NHP) animal models, which anatomical and physiological similarities to human, is important for the sake of learning the anatomical properties. This study aimed to characterize the cranial, maxillofacial, and skull base structures of non-human primates as a potential model suitable for a cranial craniotomy model. Adult Macaca fascicularis (MF) skulls classified asspecificpathogen-free for TB, SIV, SV40, Polio, Foamy virus and Herpes B virus from PT Bio Farma (Persero) Animal Lab. Library were used to represent the anatomical model.The open access database from Mammalian Crania Photographic Archive 2nd Edition (MCPA2) was used for cranial characterization analysis. This study was performed at the Department of Neurosurgery, Dr. Hasan Sadikin General Hospital and the Animal Laboratory of PT. Biofarma (Persero) from November 2018 to January 2019. The skull base structures were assessed for its analogies with its human counterpart. Comparison by t-student analysis between male and female skulls shows the mean male cranial length (CL) is greater than in female (116.68 vs 102.50 mm), with p=0.000; the mean male bizygomatic width (BZB) is greater than in female (79.30 vs 69.70 mm) with p=0.001; the mean male posterior cranial breadth (CBN) is greater than in female (63.40 vs 58.79 mm) with p=0.019; and the mean male cranial base length (CBL) is greater than in female (63.32 vs 57.55 mm), with p=0.001. The skull of MF is suitable for Neurosurgery and Neuroscience study since the MF cranial characterization and structure are similar to that of human. Its structure is ideal for performing craniotomy since it has several characteristics such as cranial vault, maxillofacial structure with huge temporal muscle, and skull base structure.  Karakteristik Kranium, Maksilofasial, dan Struktur Dasar Tengkorak Non-human Primate Macaca fascicularis dewasa: Studi Pendahuluan untuk Model Craiotomi Kranium Penggunaan hewan model berasal dari primata bukan-manusia (NHP), dimana secara anatomis dan fisiologis mirip dengan manusia, sangat penting untuk dapat mempelajari struktur anatominya. Penelitian ini bertujuan untuk karakterisasi struktur kranium, maksilofasial dan dasar tengkorak primata (Non-human Primate) yang berpotensi sebagai model penelitian kraniotomi. Tengkorak dewasa Macaca fascicularis (MF) dari  Laboratorium Hewan PT Biofarma (Persero), bebas infeksi TBC, SIV, SV40, Polio, Foamy Virus,  dan Herpes B digunakan sebagai model anatomi. Basis data terbuka Mammalian Cranial Photographic Archive 2nd Edition (MCPA2) digunakan untuk analisis kranium. Penelitian dilakukan di Departmen Bedah Saraf RSHS dan Laboratorium Hewan  PT. Biofarma  pada November 2018?Januari 2019. Struktur tengkorak MF dipelajari analogi seperti pada struktur tengkorak manusia. Analisis uji-t tengkorak jantan dan betina menunjukan rerata panjang kranial (Cranial length, CL) jantan lebih panjang dibanding betina (116.68 vs 102.50 mm, p=0.000); rerata bizygomatic width (BZB) jantan lebih panjang dibanding betina (79.30 vs 69.70 mm, p=0.001); rerata posterior cranial breadth (CBN) jantan lebih panjang dibanding betina (63.40 vs 58.79 mm, p=0.019);dan rerata cranial base length (CBL) jantan lebih panjang dibanding betina (63.32 vs57.55 mm, p=0.001). Tengkorak MF sesuai untuk berbagai penelitian bidang bedah saraf dan neurosains. Karakterisasi kranial dan strukturnya sama dengan yang dimiliki manusia. Struktur kranium MF merupakan model ideal untuk kraniotomi berdasar karakteristik tulang tengkorak, otot temporal besar pada maksilofasial, dan struktur dasar tengkorak.
CORRELATION BETWEEN SERUM MID-REGIONAL PRO-ADRENOMEDULLIN AND SEQUENTIAL ORGAN FAILURE ASSESSMENT (SOFA) SCORE IN PATIENT WITH SEPSIS Pujiyanti, Hapsari; Lismayanti, Leni; Rostini, Tiene; Parwati, Ida
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.557 KB) | DOI: 10.15395/mkb.v51n2.1456

Abstract

Most sepsis will develop into multi organ failure (MOF). To assess manifestation of MOF, SOFA score that includes several laboratory parameters for each organ is used. However, this requires time and is also costly. Recently, Mid-Regional Pro-Adrenomedullin (MR proADM) biomarkers are stated to be an alternative marker of MOF in sepsis because MR proADM is secreted by endothelials that may increase in sepsis or bacterial infection. The aim of this study was to analyze the correlation between serum MR proADM levels and SOFA score. This was a cross-sectional  observational analytical study conducted in Dr. Hasan Sadikin General Hospital (RSHS) Bandung from August 2017 to July 2018. This study was a part of the bigger sepsis biomarker study. Samples used in this study consisted of 50 stored serum from the Sepsis Biomarker study in which the MR proADM was measured. Analysis using Spearman?s correlation test showed a moderate positive correlation between serum MR proADM level and SOFA score (r = 0,582, p=0.000), showing that MR proADM serum was directly proportional to SOFA score. It is concluded that MR proADM can be considered as one of the biomarkers for multi organ failure.Korelasi Kadar Mid Regional ProAdrenomedullin Serum dengan Skor Sequential Organ Failure Assessment (SOFA) pada Penderita SepsisSebagian besar sepsis akan berlanjut menjadi multi organ failure (MOF). Untuk mengetahui MOF digunakan skor SOFA yang memerlukan beberapa parameter laboratorium untuk tiap-tiap organ dan membutuhkan waktu dengan biaya yang mahal. Saat ini diketahui biomarker Mid Regional proAdrenomedullin (MR proADM) dapat digunakan sebagai biomarker MOF pada sepsis karena MR proADM disekresi oleh endotel yang akan meningkat pada keadaan sepsis atau infeksi bakteri. Tujuan penelitian ini mengetahui korelasi kadar MR proADM serum dengan skor SOFA. Penelitian ini bersifat observasional analitik dengan rancangan potong lintang dilaksanakan di RSUP Dr. Hasan Sadikin (RSHS) Bandung pada bulan Agustus 2017 sampai Juli 2018. Penelitian ini bagian dari payung penelitian ?Biomarker pada sepsis?. Bahan pemeriksaan berupa bahan biologis tersimpan sebanyak 50 sampel dan dilakukan pemeriksaan MR proADM. Hasil analisis menggunakan Spearman?s correlation test menunjukkan korelasi positif sedang antara kadar MR proADM serum dan skor SOFA (r=0,582; p=0,000). Hal ini menunjukkan semakin tinggi kadar MR proADM serum maka semakin tinggi skor SOFA. Simpulan, terdapat korelasi positif sedang dan bermakna antara MR proADM dengan skor SOFA, sehingga MR proADM dapat dipertimbangkan sebagai salah satu biomarker pada multi organ failure.
Effect of Black Garlic Extract on Blood Glucose, Lipid Profile, and SGPT-SGOT of Wistar Rats Diabetes Mellitus Model Prihanti, Gita Sekar; Isnaini, Faradilla; Yudistia, Rieka; Faradilla, Alifa; Rahman, Masita
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1309.126 KB) | DOI: 10.15395/mkb.v51n2.1657

Abstract

Dyslipidemia is one of the complications of diabetes mellitus (DM) that can lead to elevated total cholesterol, TG, and LDL. Increased SGOT-SGPT indicates damage of hepatic cells. Black garlic extract (Allium sativum L.), which contains polyphenol, flavonoid, and anthocyanin is thought to improve blood glucose, lipid profile, and SGPT-SGOT in human. This study aimed to observe the effect of black garlic extract on blood glucose, lipid profile, and SGPT-SGOT of Wistar Rats Diabetes Mellitus Model. This study was held in 2017 at Biomedical Lab Medical Faculty of Muhammadiyah Malang University. This was a true experimental study with post test only control group design. The rats were assigned to a positive control group and 3 treatment groups consisting of 6 experimental animals each and treatment was given for 25 days. Blood glucose lipid profile, and SGPT-SGOT levels were measured by a spectrophotometer. Data were analyzed using one-way ANOVA, Post Hoc Bonferroni, and Linear Regression tests. Differences were between different groups of rats (p=0,000) with a significant difference seen between all treatment groups and the control group based on  the Post Hoc Bonferroni test results (p=0.000). Black garlic extract reduced blood sugar by 91.4%, total cholesterol by 79.1%, TG by 69.5%, LDL by  81.3%, SGPT by 91.4%, and SGOT by 70.6%. This effect is seen across doses starting from 1.5 mg/200gBW to the highest dose of 6 mg/200gBW. It can be concluded that Black garlic extract (Allium sativum L.) affects  blood glucose, lipid profile, SGPT, and SGOT positively in male wistar (Rattusnorvegicus) rats diabetes mellitus model by decreasing blood sugar, lipid profile, SGPT, and SGOT levels. Pengaruh Ekstrak Black Garlic (Allium sativum. L) Jenis  Solo Peroral terhadap Kadar GulaDarah, Profil Lipid, dan SGPT-SGOT padaTikusWistarJantan (Rattusnovergicus) Model Diabetes Melitus Dyslipidemia adalah salah satu komplikasi diabetes mellitus (DM) yang dapat berpengaruh terhadap peningkatan kolesterol total, trigliserid (TG), dan low-density lipoprotein (LDL). Peningkatan serum glutamic oksaloasetat transaminase (SGOT) dan serum glutamic pyruvic transaminase (SGPT) mengindikasikan kerusakan pada sel hepar. Ekstrak bawang putih hitam (Allium sativum L.) mengandung polifenol, flavonoid, dan anthocyanin yang dipercaya dapat memperbaiki gula darah, profil lipid, dan SGPT-SGOT pada manusia. Penelitian ini dilaksanakan pada tahun 2017 di Laboratorium Biomedik Fakultas Kedokteran Universitas Muhammadiyah Malang dan menggunakan desain eksperimental post test kelompok kontrol. Kelompok kontrol positif dan 3 kelompok perlakuan yang terdiri dari 6 hewan coba di setiap kelompok selama 25 hari. Gula darah, profil lipid, dan kadar SGPT-SGOT diukur menggunakan spektrofotometer. Data dianalisis menggunakan uji ANOVA satu arah, uji Bonferroni post hoc, dan uji regresi linear. Uji ANOVA satu arah menunjukkan ada perbedaan antarkelompok perlakuan tikus (p=0,000). Hasil uji Bonferroni post hoc menunjukkan perbedaan signifikan (p=0,000) di semua kelompok perlakuan dan kontrol. Bawang putih hitam berpengaruh 91,4% terhadap penurunan gula darah, 79,1% terhadap penurunan kolesterol total, 69,5% terhadap penurunan TG, 81,3% terhadap penurunan LDL, 91,4% terhadap kadar SGPT dan 70,6% terhadap kadar SGOT. Dosis yang memiliki efek yang signifikan sudah mulai terlihat pada dosis awal yaitu 1.5 mg/200 gBB sampai pada dosis tertinggi 6 mg/200 gBB. Dapat disimpulkan bahwa ekstrak bawang putih hitam (Allium sativum L.) terbukti mempengaruhi kadar gula darah, profil lipid, dan SGPT-SGOT tikus Wistar jantan model diabetes mellitus (Rattusnorvegicus) yaitu menurunkan gula darah, profil lipid, dan SGPT-SGOT. 
Effect of Unripe Berlin Banana Flour on Lipid Profile of Dyslipidemia Rats Agustin, Firda; Febriyatna, Ayu; Damayati, Ratih Putri; Hermawan, Hendra; Faiziah, Nurul; Santoso, Razi Dwi; Wulandari, Retno Dwi
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.289 KB) | DOI: 10.15395/mkb.v51n2.1630

Abstract

Dyslipidemia is one of the risk factors of coronary heart disease and stroke. Unripe Berlin banana flour contains chemical compounds and nutrients that may affect lipid profile. This study aimed to determine the potential effects of unripe berlin banana flour on lipid profile, especially in dyslipidemia. This experiment was conducted from July to December 2018 at the Food Analysis Laboratory of State Polytechnic of Jember and the Biomedical Laboratory of the Faculty of Dentistry, University of Jember using male wistar white rats weighed 150–200g. The rats were then divided into a negative control group consisting of  normal rats (K-), a positive control group consisting of dyslipidemia rats (K+), and a treatment group consisting of dyslipidemia rats that received 0.144 g unripe berlin banana flour (UBF). Dyslipidemia condition was achieved by administering high-fat diet (HFD) for 9 weeks. Unripe banana flour was fed to the treatment group starting from the 10th weeks for  4 weeks. The measurement of pre-intervention lipid profile was performed at 9th week while the post-intervention lipid profile was measured at 13th week. Data collected were analyzed using ANOVA. Results o showed that the Berlin unripe flour (UBF) could reduce total cholesterol and LDL levels. In addition, a decrease in HDL levels in treatment group  with Berlin unripe banana flour (UBF) and in the negative control group (K-). The same decrease in HDL level was also seen in the dyslipidemia group (K+). Hence, it is concluded that Berlin banana flour has the potential to improve lipid profile in dyslipidemia rats. Pengaruh Pemberian Tepung Pisang Berlin Unripe terhadap Perbaikan Profil Lipid Tikus Dislipidemia  Dislipidemia merupakan faktor risiko penyakit jantung koroner dan strok. Tepung pisang berlin unripe mengandung senyawa aktif dan zat gizi yang berpengaruh terhadap profil lipid. Penelitian ini bertujuan mengetahui potensi tepung pisang berlin unripe terhadap profil lipid pada tikus jantan galur wistar model displidemia. Penelitian dilaksanakan pada bulan Juli sampai dengan Desember 2018 di Laboratorium Analisis Pangan, Politeknik Negeri Jember dan Laboratorium Biomedik, Fakultas Kedokteran Gigi, Universitas Jember. Penelitian menggunakan tikus jantan galur Wistar dengan bobot badan 150–200 gr. Kelompok perlakuan terdiri atas kelompok tikus normal (K-), tikus dislipidemia (K+), tikus dislipidemia + tepung pisang Berlin unripe dosis 0,144 gram (UBF). Perlakuan dislipidemia dilakukan dengan cara pemberian  high fat diet (HFD) selama 9 minggu. Selanjutnya, pemberian UBF diberikan dalam bentuk pakan selama 4 minggu. Pengukuran profil lipid pre-intervensi (minggu ke-9) dan post-intervensi (minggu ke-13). Data dianalisis dengan uji ANOVA. Hasil penelitian menunjukkan bahwa kelompok perlakuan tepung pisang Berlin unripe (UBF) dapat menurunkan kadar kolesterol total dan LDL. Selain itu, terjadi penurunan kadar HDL pada kelompok perlakuan tepung pisang Berlin unripe (UBF) dan kelompok negatif (K-). Pada kelompok K+ juga terjadi penurunan kadar HDL. Simpulan penelitian, tepung pisang Berlin memiliki potensi terhadap perbaikan profil lipid pada tikus dislipidemia.
Use of Propranolol in Infantile Hemangioma Resulting in Scarless Healing Vania, Rachel; Irwansyah, Denny
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.267 KB) | DOI: 10.15395/mkb.v51n2.1538

Abstract

Infantile hemangiomas (IHs) are the most common childhood tumors. Some can ulcerate causing pain, bleeding, and scarring, leading to cosmetical disfigurement. The use of propranolol has beaten the traditional high-dose systemic corticosteroids therapy, which falls short on its infamous adverse effects. In these case series, regimen of propranolol was highlighted when surgery is not an option. Two cases were presented. Case 1 involveda three-month-old baby girl was presented with a 0.6x0.6 cm disfiguring purple tumor on her nasal tip and columella and Case 2 involveda three-month-old baby girl with a 2x0.5 cm sharply bordered and bulging red tumor on her left labium major.Both lesions became visible at the age of 3 months and grew rapidly since then.  Propranolol was initiated at 2 mg/kgBW per day with careful administration. Adverse effects and drug tolerance were closely monitored under pediatrician and plastic surgery care with none reported. Dramatic results was seen after 2 weeks of treatment and no scar was found at the end of the therapy. This confirmed that propranolol is an effective and well tolerated treatment when commenced early and continued with the minimum protocol of 2 mg/kgBW/day through the proliferative phase of infantile hemangioma. It is concluded that propranolol is beneficial for managing infantile hemagioma cases with a scarless outcome that cannot be achieved through surgery. The use of propanolol is extremely beneficial when the case involves a tumor in a site that makes it impossible for reconstruction. Penggunaan Propranolol pada Hemangioma Infantil yang Menghasilkan Penyembuhan Tanpa Bekas LukaHemangioma infantil adalah tumor yang paling sering pada anak. Tumor ini dapat mengalami ulserasi, nyeri, perdarahan, parut buruk, sampai menyebabkan penampilan cacat. Terapi propranolol telah mengalahkan kortikosteroid, yang dikenal mempunyai banyak efek samping yang buruk. Pada serial kasus ini, kami akan menyoroti regimen penggunaan propranolol, ketika pembedahan tidak mungkin dilakukan. Kasus 1: Bayi perempuan, 3 bulan, datang dengan tumor keunguan yang menyebabkan disfigurasi pada nasal tip dan kolumela. Kasus 2: Bayi perempuan, 3 bulan, datang dengan tumor kemerahan, berbatas tegas dan benjol, pada labium mayor sinistra. Kedua lesi timbul pada usia 3 bulan dan membesar dalam hitungan hari. Propranolol dimulai dengan dosis 2 mg/kg/BB per hari, dengan pengawasan ketat oleh spesialis anak dan bedah plastik. Kedua kasus tidak terdapat efek samping dan toleransi obat baik. Hasil drastis terlihat dalam dua minggu dan pada akhir terapi didapatkan parut yang kasat mata. Kasus kami mengonfirmasi bahwa terapi propranolol adalah efektif dan dapat ditoleransi dengan baik, diberikan dini dan stabil pada dosis 2 mg/kg/BB per hari pada fase proliferasi, dengan hasil tanpa bekas yang tidak dapat dicapai dengan pembedahan. Kami ingin meningkatkan kesadaran pada dokter dalam penggunaan propranolol yang telah dibuktikan bermanfaat, terutama pada tumor yang berada di tempat yang tidak dapat dioperasi dan sulit direkonstruksi.
Antioxidant and Antityrosinase Activities of Ethanolic Pachyrhizuserosus Peel and Tuber Extract Siregar, Irma Dolsyeria; Kusuma, Hanna Sari Widya; Widowati, Wahyu; Marpaung, Hans Hotma; Ferdinand, Sahna; Fachrial, Edy; Lister, I Nyoman Ehrich
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.515 KB) | DOI: 10.15395/mkb.v51n2.1628

Abstract

Aging process is a physiological process in living organisms caused by, among others, free radicals. One of the free-radical-related aging problems is skin hyperpigmentation (excessive melanin) due to increasing tyrosinase enzyme activities. Natural compounds are widely used as antioxidant and antiaging agents. Bengkuang (Pachyrhizuserosus) is known as a source of various active compounds which can be used against free radicals to reduce the risk of skin aging  through  tyrosinase enzyme inhibition. This study was performed in September 2018 in Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia to examine the antioxidant and antityrosinase properties of Pachyrhizuserosus peel extract (PPE) and Pachyrhizuserosus  tuber extract (PTE).The extraction of PPE and PTE was performed using 70% ethanol by maceration method, followed by  phytochemical analysis using modified Farnsworth method. Antioxidant activities were measured through 2,2-Diphenyl-1-picrylhydrazil (DPPH) scavenging activities while antiaging assay were conducted through the  tyrosinase activity inhibition. In this study, PPE contained saponin, tannin, triterpenoid, and terpenoid while the PTE showed the presence of flavonoid, saponin, phenol, tannin, and alkaloid in phytochemical analysis. In the antioxidant assay, PPE presented a higher DPPH scavenging activities (IC50= 84.09 µg/mL) when compared to PTE (IC50= 98.30 µg/mL)(p<0.05). In antiaging assay, PPE showed a higher tyrosinase inhibitory activities when compared to PTE with =97.05µg/mL and 194.51µg/mL,respectively. It can be concluded that PPE has antioxidant and antiaging activities effective for preventing skin aging. Aktivitas Antioksidan dan Antitirosinase Ekstrak Etanol Kulit dan Daging PachyrhizuserosusProses penuaan merupakan suatu proses fisiologis yang terjadi pada makhluk hidup yang dapat disebabkan oleh radikal bebas. Hiperpigmentasi kuli tmerupakan salah satu masalah penuaan yang disebabkan oleh radikal bebas melalui peningkatan aktivitas enzim tirosinase. Bengkuang (Pachyrhizus erosus) diketahui mengandung berbagai senyawa aktif yang dapat menangkal radikal bebas serta mengurangi risiko penuaan kulit. Penelitian dilaksanakan pada September 2018 di Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, Indonesia untuk menguji kemampuan aktivitas antioksidan dan antitirosinase pada ekstrak etanol kulit bengkuang (EEKB) dan ekstrak etanol daging bengkuang (EEDB). Pembuatan ekstrak kulit dan daging bengkuang dilakukan dengan cara mengekstraksi bahan dengan etanol 70% menggunakan metode maserasi, kemudian dilanjutkan dengan analisis fitokimia ekstrak dengan modifikasi metode Farnsworth. Aktivitas antioksidan diuji dengan mengukur pemerangkapan 2,2-Diphenyl-1-picrylhydrazil (DPPH) sedangkan antiaging diuji dengan mengukur aktivitas penghambatan tirosinase. Pada uji fitokimia menunjukkan EEKB memiliki kandungan senyawa saponin, tanin, triterpenoid dan terpenoid, sedangkan EEDB menunjukkan kandungan senyawa flavonoid, saponin, fenol, tanin, dan alkaloid. Pada uji antioksidan, EEKB memiliki aktivitas tertinggi pada pemerangkapan DPPH (IC50=84.09 µg/mL) dibanding dengan EEDB (IC50=98.30 µg/mL) (p≤0.05). Pada pengujian antiaging, EEKB memiliki nilai yang lebih tinggi pada aktivitas penghambatan tirosinase dibandingkan dengan EEDB (IC50=97.05 µg/mL; 194.51 µg/mL (p≤0.05). Simpulan, EEKB memiliki aktivitas antioksidan dan antiaging sehingga efektif dalam mencegah penuaan kulit. 
Role of Physical and Cognitive Factors in Passing Abilities of Futsal Athletes in Universitas Padjadjaran Lubis, Leonardo; Muhammad, Afan Maulana; Nandina, Oktavia
Majalah Kedokteran Bandung Vol 51, No 2 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n2.1578

Abstract

Futsal is a variation of soccer, to be played on a smaller field, with the fastest development among other indoor sports. Futsal players need to master several basic playing techniques to be able to play good futsal, one of them is passing. There are 2 factors that affect futsal athlete skills; physical and mental factors, including cognitive factors. This study aimed to determine the contribution of physical and cognitive factors on the ability to execute passing techniques. A cross-sectional analytic study was conducted in October 2018 on 33 participants who were listed as futsal players in the Faculty of Medicine Futsal Unit, Universitas Padjadjaran, and in the amateur futsal club, La Coruna FC. Physical factor data consisting of body muscle mass percentage, leg muscle percentage, total body fat percentage, and legs subcutaneous fat percentage were measured using Body Impedance Analysis tool while the cognitive factors consisting of; attention, short term memory, and spatial ability, were measured using grid concentration test, digit span test, and spatial ability test, respectively. Athlete’s passing ability was measured using 30 second pass and stop test. Data were then analyzed using regression analysis. The results showed that the percentage of leg muscles has the highest significance value for the athlete’s passing ability. (β=0.6, p<0.05) while the leg muscles percentage and attention level are shown to be the physical and cognitive factors which play greater roles in the passing ability of futsal athletes.Peran Faktor Fisik dan Kognitif  terhadap Performa Teknik Dasar Passing Atlet Futsal Universitas Padjadjaran Futsal adalah variasi sepakbola yang dimainkan di lapangan yang lebih kecil. Atlet futsal perlu menguasai beberapa teknik bermain dasar untuk bermain futsal dan salah satunya adalah passing. Ada 2 faktor yang memengaruhi keterampilan atlet futsal, yaitu fisik dan mental yang salah satunya adalah kognitif. Penelitian ini bertujuan mengetahui kontribusi faktor fisik dan kognitif terhadap kemampuan teknik passing. Studi analitik cross-sectional dilakukan terhadap 33 peserta yang terdiri dari atlet futsal Fakultas Kedokteran dan atlet futsal klub amatir, La Coruna FC selama Oktober 2018 di Jatinangor. Data komponen fisik terdiri atas persentase massa otot tubuh, persentase otot tungkai, persentase lemak tubuh total, dan persentase lemak subkutan kaki yang diukur dengan alat analisis impedansi tubuh, sedangkan kognitif terdiri atas konsentrasi, memori jangka pendek, dan kemampuan spasial yang masing-masing diukur dengan uji konsentrasi grid, uji rentang digit, dan uji kemampuan spasial. Kemampuan passing diukur menggunakan tes pass and stop selama 30 detik. Data kemudian diproses dengan analisis regresi. Hasil Analisis regresi menunjukkan persentase otot kaki memiliki nilai signifikansi tertinggi untuk kemampuan passing atlet. (β=0,6, p<0,05). Persentase otot tungkai dan tingkat konsentrasi adalah faktor fisik dan kognitif yang memiliki peran lebih besar dalam kemampuan passing atlet futsal Jatinangor. 

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