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Majalah Kedokteran Bandung
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Comparison of Antioxidant and Antiaging Activities Between Dragon Fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose) Rind Extract and Kaempferol Viany, Lisma; Rizal, Rizal; Widowati, Wahyu; Samin, Buter; Kusuma, Rita; Fachrial, Edy; I Nyoman, Lister Ehrich
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1715

Abstract

Aging involves progressive physiological changes of the body, including skin dysfunction and death which can be accelerated by the presence of free radicals. Compounds with potential antioxidants and antiaging are contained in various fruits, including dragon fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose). This study aimed to compare the antioxidant and antiaging activities of dragon fruit rind extract (DFRE) and Kaempferol. The antioxidant and antiaging activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenger and inhibitory activity assay of tyrosinase, respectively and was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama, from August to November 2018. Data acquired were then analyzed using ANOVA, followed by Post Hoc Test using Turkey HSD test with a confidence level of 95% (α=0.05). Phytochemical assay was also conducted to determine the content of active ingredients in thedragon fruit rind extract. Phytochemical test result showed that DFRE contained flavonoids, tannins, phenols, triterpenoids, and alkaloids compounds. Both DPPH scavenging and tyrosine inhibition assay indicated that Kaempferol compound also had antioxidant and antiaging activities. The IC50 values of DFRE and Kaempferol antioxidant activities were 164.98 μg/mL and 83.30 μg/mL, respectively, while the IC50 values of tyrosinase inhibition activity of DFRE and Kaemferol were 88.46 μg/mL and 59.34 μg/mL, respectively. DFRE had antioxidant activities and tyrosinase inhibition activities thus can be used as an alternative antiaging and antioxidant agents. Perbandingan Aktivitas Antioksidan dengan Antitirosinase Ekstrak Etanol Kulit Buah Naga (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose)Aging atau penuaan adalah proses kompleks yang ditandai dengan penurunan progresif fungsi fisiologis tubuh, termasuk kulit yang diikuti oleh disfungsi dan kematian kulit. Faktor-faktor yang dapat mempercepat proses penuaan dini di antaranya radikal bebas dan gaya hidup yang tidak sehat. Buah-buahan banyak mengandung senyawa yang berpotensi sebagai antioksidan dan antiaging, salah satu di antaranya adalah buah naga. Penelitian ini bertujuan mengetahui potensi antioksidan dan antiaging ekstrak kulit buah naga (EKBN) yang dibandingkan dengan senyawa kaempferol (SK) sebagai pembanding. Penelitian yang dilakukan meliputi uji fitokimia untuk mengetahui kandungan bahan aktif pada kulit buah naga, uji pemerangkapan DPPH, dan uji penghambatan tirosinase dan dilakukan di Pusat Penelitian Biomolekul dan Biomedis, Aretha Medika Utama, dari Agustus hingga November 2018. Data lalu dianalisis menggunakan analisis varians (ANOVA) satu arah (one way) Hasil penelitian uji fitokimia menunjukkan EKBN mengandung golongan senyawa, flavonoid, tanin, fenol, triterpenoid, dan alkaloid serta tidak mengandung golongan senyawa saponin, steroid, dan terpenoid. Senyawa kaempferol memiliki aktivitas antioksidan melalui pemerangkapan DPPH dan aktivitas antiaging melalui penghambatan tirosinase yang lebih baik dibanding dengan EKBN. Aktivitas antioksidan dilihat dari nilai IC50 EKBN sebesar 164.98 μg/ml dan SK sebesar 83.30 μg/mL. Aktivitas penghambatan tirosinase memiliki nilai IC50 EKBN masing-masing sebesar 88.46 μg/mL dan IC50 SK sebesar 59.34 μg/mL. EKBN memiliki aktivitas antioksidan dan penghambatan tirosinase sehigga dapat dimanfaatkan sebagai alternatif bahan antiaging dan antioksidan. 
The Correlation Between Neutrophil-lymphocyte Count Ratio and Procalcitonin in Septic and Septic Shock Jonathan, Jonathan; Pradian, Erwin; Zulfariansyah, Ardi
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.35 KB) | DOI: 10.15395/mkb.v51n3.1642

Abstract

Sepsis is one of the leading causes of death in patients with infection. Procalcitonin is one of the laboratory parameters that is used to differentiate between thesystemic inflammatory responses caused by pathogens and non-pathogens. Neutrophil-lymphocyte count ratio (NLCR) is one of the simple laboratory parameters used. The purpose of this study was to investigate the correlation between NLCR and procalcitonin for sepsis and septic shock patients. A cross-sectional prospective correlational analysis study was perfromed on 36 sepsis and septic shock patients admitted to the Intensive Care Unit of Dr. Hasan Sadikin Bandung General Hospital during September 2017–May 2018. Statistical analysis was performed using Rank Spearman’s and Mann Whitney test. Both procalcitonin and NLCR increased in sepsis and septic shock patients. Procalcitonin level was significantly different in sepsis and septic shock groups (p<0.05), albeit insignificant difference in NLCR (p>0.05). NLCR was significantly higher in gram positive bacteria patient group (p<0,05); however, the difference was not seen for procalcitonin level (p>0.05). Correlation test found a significant positive correlation between SOFA score and procalcitonin (R=0.557 and p<0.01) and no correlation between SOFA score and NLCR (R=0.117 and p>0.05). There was no correlation between NLCR and procalcitonin for sepsis and septic shock patients (R=0.122 and p>0.05). Therefore, no correlation was found between NLCR and procalcitonin for sepsis and septic shock patients.Korelasi antara Rasio Hitung Neutrofil-Limfosit dan Prokalsitonin pada Pasien Sepsis dan Syok SepsisSepsis merupakan salah satu penyebab kematian terbanyak pada pasien infeksi. Pemeriksaan prokalsitonin merupakan salah satu parameter untuk membedakan bahwa respons inflamasi sistemik disebabkan oleh patogen atau non-patogen. Parameter laboratorium lain yang lebih sederhana adalah rasio hitung neutrofil-limfosit (neutrophil-lymphocyte count ratio/NLCR). Tujuan penelitian ini mengetahui korelasi antara NLCR dan prokalsitonin pada pasien sepsis dan syok sepsis. Penelitian analisis korelasional prospektif ini dilakukan dengan desain potong lintang terhadap 36 pasien sepsis dan syok septik yang dirawat di ICU Rumah Sakit Dr. Hasan Sadikin Bandung periode September 2017–Mei 2018. Analisis statistik menggunakan Uji Rank Spearman dan Mann Whitney. Kadar prokalsitonin dan NLCR meningkat pada pasien sepsis dan syok septik. Kadar prokalsitonin berbeda secara signifikan pada kelompok sepsis dan syok septik (p<0,05), perbedaan tidak terlihat untuk NLCR (p>0,05). NLCR secara signifikan lebih tinggi pada bakteri gram positif daripada bakteri gram negatif (p<0,05), perbedaan tidak terlihat pada kadar prokalsitonin (p>0,05). Uji korelasi menunjukkan korelasi positif yang signifikan antara skor SOFA dan prokalsitonin (R=0,557; p<0,01) dan tidak terdapat korelasi antara skor SOFA dengan NLCR (R=0,117; p>0,05). Tidak terdapat korelasi antara NLCR dan prokalsitonin pada pasien sepsis dan syok septik (R=0,122; p> 0,05). Simpulan: tidak terdapat korelasi antara NLCR dan prokalsitonin pada pasien sepsis dan syok septik.
COMPARISON OF HBA1C LEVELS IN PATIENTS WITH TB-DM AND TB-NON DM Puetri, Nona Rahmaida; Hadifah, Zain; Nur, Abidah; Hanum, Sari
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.808 KB) | DOI: 10.15395/mkb.v51n3.1753

Abstract

Tuberculosis (TB) is still a global problem that has not currently been resolved, due to it rapid transmission through droplet nuclei released when TB patients cough, sneeze, or even talking. One of the TB comorbidities is Diabetes Mellitus (DM) that makes it necessary to do HbA1c screening in patients with TB DM and TB non-DM to prevent further disease complications.This was a cross-sectional descriptive analytic study conducted in 2018 on a total sample of 248 TB-DM and TB non-DM patients in Aceh Besar District and Banda Aceh City, Indonesia with 29.8% of the subjects were TB-DM patients and the remaining 70.2% were TB Non-DM patients. The.HbA1c levels in TB patients varied, with the average HbA1c level of 10.738 in people with TB DM and 5.426 in TB non-DM. The HbA1c level in patients with TB DM was two times higher than in TB non-DM patients. Around 21% of TB patients who were not diagnosed with DM by health care workers showed an HbA1c level that was in line with the level in the DM category. Therefore, it is recommended that each TB patient undergoes screening HbA1c in order to prevent TB non-DMpatients from becoming TB DM patients.
CORRELATION BETWEEN ANKLE-BRACHIAL INDEX SCORE AND DIABETIC POLYNEUROPATHY DEGREE OF SEVERITY Gamayani, Uni; Miftahurachman, Miftahurachman; Lailiyya, Nushrotul; Juhana, Handika Sonjaya
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1543

Abstract

Diabetic polyneuropathy (DPN) is the most prevalent chronic microvascular complication of type 2 diabetes mellitus (DM). The severity of DPN is categorized based on symptoms, signs, and nerve conduction study (NCS) abnormality into grades 0, 1a, 2a, and 2b. Pathogenesis of DPN consists of metabolic and vascular processes. In addition, macrovascular factor also plays a role in the pathogenesis of DPN. Macrovascular diseases, such as peripheral arterial disease (PAD), could be diagnosed using the ankle brachial index (ABI). Several studies have proven a strong correlation between ABI and DPN; hence, this study aimed to examine the correlation between ABI score and DPN severity. This was a cross sectional analytic study on. 73 type 2 DM patients who met the inclusion and exclusion criteria and visited the outpatient Endocrine Clinic and Neurophysiology laboratory of the Department of Neurology Dr. Hasan Sadikin General Hospital Bandung, during the priod of June to October 2018. ABI examination were performed after DPN indication was evident from results of physical examination, lab tests, and NCS. Rank Spearman statistical analyses were performed to assess the correlation between ABI score with DPN severity and the result was considered significant if p value <0.05. No correlation was found between ABI score DPN severity and between ABI score and DPN symptoms, signs, and NCS abnormality. Hence, ABI score does not correlate with DPN severity stage. Hubungan Skor Ankle-Brachial Index dengan Derajat Keparahan Polineuropati DiabetikaKomplikasi kronis mikrovaskular diabetes mellitus (DM) tipe 2 yang sering ditemukan adalah polineuropati diabetika (PND). Derajat keparahan PND berdasar gejala, tanda, dan abnormalitas pemeriksaan konduksi saraf tepi (KST) dibagi atas derajat 0, 1a, 2a, dan 2b. Patogenesis PND terdiri atas proses metabolik dan vaskular. Faktor makrovaskular diduga memiliki peranan terhadap terjadinya PND. Penyakit arteri perifer oklusif (PAPO) adalah penyakit makrovaskular yang dapat dideteksi dengan pemeriksaan ankle brachial index (ABI). Beberapa penelitian membuktikan hubungan antara skor ABI dan PND. Penelitian ini bertujuan menilai hubungan antara skor ABI dan derajat keparahan PND. Penelitian bersifat observasi analitik studi potong lintang yang dilakukan pada 73 penyandang DM tipe 2 yang memenuhi kriteria inklusi dan eksklusi di Instalasi Rawat Jalan Klinik Endokrin dan Laboratorium Neurofisiologi KSM/Departemen Neurologi RSUP Hasan Sadikin Bandung periode Juni sampai Oktober 2018. Pemeriksaan ABI dilakukan setelah pemeriksaan klinis PND, laboratorium darah, dan KST. Korelasi antara skor ABI dengan derajat keparahan PND dinilai menggunakan analisis statistik Rank Spearman, signifikan jika p <0,05. Tidak terdapat korelasi antara skor ABI dan derajat keparahan PND. Tidak terdapat korelasi gejala PND, tanda PND, maupun abnormalitas uji KST dengan skor ABI. Skor ABI tidak berkorelasi dengan derajat keparahan PND, baik gejala PND, tanda PND, maupun abnormalitas uji KST. 
HEPATOPROTECTIVE AND ANTIFIBROTIC EFFECTS OF INDONESIAN PROPOLIS Prasetyo, Diding Heri; Sarsono, Sarsono; Nurwati, Ida; Putranto, Prihandjojo Andri; Martini, Martini; Prasetyo, Nabila Aushaf
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.973 KB) | DOI: 10.15395/mkb.v51n3.1768

Abstract

Liver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect of ethanol extract of propolis (EEP) from mount Lawu, Indonesia to prevent liver damage and fibrosis progression in mice with hepatic cirrhosis. This study was performed during the period of June 2018 to May 2019 on a sample of 32 male Balb/C mice divided into control group (P1), induction of carbon tetrachloride (CCl4 ) group (P2), induction of 50 mg/BW CCl4 + EEP group (P3), and (induction of 100 mg/KgBW CCl4 + EEP (P4) with each group consisted of eight mice. The CCl4 in olive oil was administered intraperitoneally three times a week for six weeks. Mean differences between group was determined using ANOVA test with a significance level of 0.05. The induction of CCl4 increased liver cell damage and serum alanin aminotransferase (ALT) level. However, the addition of EEP significantly (p<0.001) reduced liver cell damage as seen in P3 (54.38±4.17 per 100 liver cells) and P4 (37.13±4.36 per 100 liver cells) groups and serum alanin aminotransferase (ALT) as seen in P3 (291.19±113.92 U/L) and P4 (229.38±73.45 U/L) groups. The APRI scores were also reduced after EEP as seen in P3 (0.738±0.292) and P4 (0.513±0.253) groups. Thus, EEP isolates from Gunung Lawu can reduce liver cell damage and fibrosis in mice model of hepatic cirrhosis. Efek Hepatoprotektif dan Antifibrotik Propolis IndonesiaSirosis hati (SH) merupakan tahap ireversibel dalam proses kerusakan hati yang terjadi setelah fibrosis hati sebagai hasil aktivitas nekroinflamasi dan fibrosis hati. Oleh karena itu, penghambatan inflamasi hati dan fibrosis sangat penting untuk mencegah terjadi SH. Penelitian ini bertujuan menganalisis efek ekstrak etanol isolat propolis (EEP) gunung Lawu dalam mencegah progresifitas kerusakan dan fibrosis hati pada mencit model SH. Penelitian dilakukan di Laboratorium Histolgis Fakultas Kedokteran Universitas Sebelas Maret Surakarta dan Lembaga Penelitian dan Pengujian Terpadu (LPPT) Universitas Gajah Mada Yogyakarta . Penilitian ini dilakukan periode Juni 2018 sampai Mei 2019, dengan sampel 32 ekor mencit Balb/C jantan dibagi menjadi kelompok (P1) kontrol negatif, (P2) induksi karbon tetraklorida (CCl4 ), (P3) induksi CCl4 +EEP dosis 50 mg.kgBB-1, dan (P4) induksi CCl4 +EEP dosis 100 mg.kgBB-1, dengan tiap-tiap sampel terdiri dari delapan ekor mencit Balb/C jantan. CCl4 dalam minyak zaitun diberikan pada tikus Balb/C tiga kali seminggu selama enam minggu secara intraperitoneal. Uji ANOVA digunakan untuk menentukan perbedaan rata-rata antar kelompok dengan tingkat kemaknaan sebesar 0,05. Namun, penambahan EEP secara signifikan (p<0,001) menurunkan tingkat kerusakan sel hati seperti yang terlihat pada P3 (54,38±4,17 per 100 sel hati) dan kelompok P4 (37,13±4,36 per 100 sel hati) dan serum alanin aminotransferase (ALT) seperti yang terlihat dalam kelompok P3 (291,19±113,92 U / L) dan P4 (229,38±73,45 U / L). Hasil skor APRI menunjukkan kelompok P1(0,213±0,113) dan P2 (0,863±0,534), EEP dapat menurunkan skor APRI pada kelompok P3 (0,738±0,292) dan P4 (0,513±0,253). Simpulan, EEP isolat Gunung Lawu menurunkan tingkat kerusakan sel hati dan fibrosis pada mencit model SH.
Correlation between Body Mass Index and Serum Leptin Level in Patients with Polycystic Ovary Syndrome Jupri, Erna; Tjandrawati, Anna; Bayuaji, Hartanto; Rachmayati, Sylvia; Parwati, Ida
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1459

Abstract

Polycystic ovary syndrome (PCOS) is the most common endocrine abnormality in reproductive-age women, which is characterized by hyperandrogenism, anovulation, and polycystic ovaries. Some evidence suggested that leptin also causes PCOS due to its role in female reproductive system. The physiological function of leptin controls the balance of energy and suppresses the center of appetite. Patients with PCOS maybe underweight, normoweight, overweight, and obese, based on their body mass index (BMI), but obesity is a common clinical situation in PCOS. Obesity occurs when the level of leptin increases but cannot decrease appetite, resulting in leptin resistance. This study aimed to discover the connection between BMI and the level of leptin in patients with PCOS. This was a cross-sectional observational analytic performed in Dr. Hasan Sadikin General Hospital Bandung between April 25 and July 10, 2018. Subjects included 46 PCOS patients who were diagnosed based on Rotterdam criteria by gynecology obstetricians in the hospital. Enzyme-linked immunosorbent assay (ELISA) was used to examine the leptin serum levels. Leptin level increased 74%, mostly in obese patients that comprised 41% of these subjects. Normal leptin levels was seen in 26% of patients, none of them was obese. The Spearman Rank correlation test showed a significant positive correlation between BMI and leptin levels (r=0.644, p=0.000). Hence, a high body mass index in people with PCOS positively correlates with increased leptin level. Korelasi antara Indeks Massa Tubuh dan Kadar Leptin Serum pada Penderita Sindrom Ovarium PolikistikSindrom Ovarium Polikistik (SOPK) merupakan kelainan endokrin pada wanita reproduktif yang ditandai oleh hiperandrogenisme, anovulasi, dan ovarium polikistik. Terdapat beberapa bukti bahwa leptin turut menyebabkan SOPK karena leptin berperan dalam sistem reproduksi wanita. Fungsi fisiologis leptin mengendalikan keseimbangan energi dan menekan pusat nafsu makan. Penderita SOPK dapat memiliki indeks massa tubuh (IMT) underweight, normoweight, overweight dan obese, namun obesitas merupakan gambaran klinis yang umum terjadi pada SOPK. Obesitas terjadi bila peningkatan kadar leptin, namun leptin tidak mampu bekerja menurunkan nafsu makan sehingga terjadi resistensi leptin. Penelitian ini bertujuan mengetahui hubungan IMT dengan kadar leptin pada penderita SOPK. Penelitian ini berbentuk analitik observasional dengan rancangan potong lintang dan dilakukan di Rumah Sakit Umum Pusat Dr. Hasan Sadikin (RSHS) Bandung pada tanggal 25 April–10 Juli 2018. Subjek penelitian sebanyak 46 penderita SOPK yang didiagnosis berdasar atas kriteria Rotterdam oleh klinisi obstetri ginekologi RSHS. Pemeriksaan kadar leptin serum mengunakan metode Enzyme-linked immunosorbent assay (ELISA). Kadar leptin yang meningkat 74% terbanyak pada IMT obese 41% subjek, dan kadar leptin normal 26% tidak ditemukan pada subjek yang IMT obese. Uji korelasi Rank Spearman menunjukkan korelasi positif kuat yang bermakna antara IMT dan kadar leptin (r=0,644; p=0,000). Indeks massa tubuh yang tinggi pada penderita SOPK berkorelasi positif dengan peningkatan kadar leptin.
Effect of Serial Passage on Growth Kinetics, Biological Properties, and Differentiation into Adipocytes of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells Rizal, Rizal; Widodo, Wahyu Setia; Wibowo, Satrio; Munshy, Ubaydillah Zedd
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.372 KB) | DOI: 10.15395/mkb.v51n3.1767

Abstract

Early passges of human Wharton’s jelly-derived mesenchymal stem cells (hWJMSCs) have been suggested by various studies; however, the effect of serial passaging has not been well defined. This study aimed to investigate the effect of serial passage on the growth kinetics, biological properties and differentiation into adipocytes on hWJMSCs. This study was conducted from April to December 2014 to evaluate the hWJMSCs growth kinetics at passage 6 (P6) and P12 by calculating proliferation rate, population doubling time (PDT), and cumulative population-doubling levels (CPDL) using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium MTS assay kit. The cells biological properties were characterized using flow cytometry analysis followed by measuring the differentiation into adipocytes in extensive culture using Oil Red O staining after induction. An independent sample t-test was applied to analyze the differences between groups using IBM SPSS Statistics Version 22 with a p<0.05 considered significant. The growth kinetics assay showed the cells at P6 had a higher proliferation rate, CPDL, and faster PDT compared to the cells at P12 (p<0.05). Data from expression of MSCs surface markers showed that the cells retained their biological properties at both passages but decreased at P12 (p<0.05). The formation of intracellular lipid droplets was also observed at both passages under adipogenic induction. However, the content of oil red O extracted-cells decreased at P12 (p<0.05). The hWJMSCs decreased their growth kinetics, biological properties, and differentiation into adipocytes as a result of serial passage. This finding might be valuable for treating degenerative diseases using cell-based therapy. Pengaruh Pasase Berseri terhadap Kinetika Pertumbuhan, Karakteristik Biologis, dan Diferensiasinya Menjadi Adipocytes yang Berasal dari Jaringan Wharton’s Jelly-Sel Punca MesenkimPenelitian tentang sel punca mesenkim yang berasal dari jaringan Wharton’s Jelly manusia (hWJMSCs) hanya disarankan penggunaanya pada pasase awal. Pengaruh pasase berseri terhadap karakteristik sel hWJMSCs belum banyak diuraikan dengan baik. Penelitian ini bertujuan untuk mengetahui efek dari pasase berseri terhadap kinetika pertumbuhan, sifat biologis dan diferensiasi menjadi adipocytes dari sel hWJMSCs. Penelitian ini dilakukan di Laboratorium Fisiologi, Fakultas Kedokteran, Universitas Gadjah Mada dan Aretha Medika Utama, Biomolecular and Biomedical Research Center, dari bulan April sampai Desember 2014. Kinetika pertumbuhan sel hWJMSCs pada P6 dan P12 dievaluasi dengan menghitung laju pertumbuhan sel, population doubling time (PDT), dan cumulative population-doubling levels (CPDL) menggunakan uji MTS. Sifat biologis sel dikarakterisasi menggunakan flowsitometri dengan mengukur ekspresi penanda permukaan CD90, CD105, CD73, dan CD44. Kemampuan diferensiasinya menjadi adipocytes diukur menggunakan pewarnaan Oil Red O setelah induksi mendium diferensiasi adipogenik. Uji kinetika pertumbuhan sel menunjukkan sel-sel di P6 memiliki tingkat proliferasi dan CPDL yang lebih tinggi dan PDT yang lebih cepat dibanding dengan sel-sel P12 (p<0,05). Karakterisasi MSCs menunjukkan bahwa sel-sel tersebut mempertahankan sifat biologisnya di kedua pasase tetapi menurun pada P12 (p<0,05). Jumlah intraseluler lemak pada P12 lebih sedikit dibandingkan pada P6 (p<0,05). Kinetika pertumbuhan, sifat biologis, dan kemampuan berdiferensiasi menjadi adipocytes dari hWJMSCs menurun sebagai akibat pasase berseri. 
Sindrom Patau dengan genotipe 47,xy,+13,t(13:18) Elfira, Vanda; Kadi, Fiva Aprilia; Laksono, Bremmy; Effendi, Sjarif Hidajat
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1389

Abstract

Trisomy 13 (Patau syndrome) is cytogenetically classified as a 47,XY,+13 or 47,XX,+13, due to nondisjunction at meiosis I or II, or at mitosis (mosaicism), and partial trisomy due to translocation. Patau syndrome is one of the most common chromosomal anomalies with an estimated incidence of about 1/10,000 births characterized by the presence of cleft lip and/or palate, post axial polydactyly, low set ears, rocker-bottom feet, cryptorchidism, and congenital heart disease. This was a case report of a newborn baby in Dr. Hasan Sadikin General Hospital Bandung in January 2016 with translocation of chromosome 13 segment to chromosome 18 or 47,XY,+13,t(13:18). Sindrom Patau dengan Genotype 47,XY,+13,t(13:18)Trisomi 13 (sindrom Patau) secara sitogenetik diklasifikasikan 47,XY,+13 atau 47,XX,+13, disebabkan oleh kegagalan pemisahan pada meiosis I atau II, atau pada mitosis (mosaicism) dan trisomi parsial yang disebabkan oleh translokasi. Sindrom Patau merupakan salah satu anomali kromosom yang paling sering dengan insidensi sekitar 1/10.000 kelahiran, dengan karakteristik fenotipe seperti celah bibir dan atau celah langit-langit, postaxial polydactyly, low set ears, rocker bottom feet, kriptokismus, serta kelainan jantung kongenital. Dilaporkan kasus sindrom Patau pada bayi baru lahir di Rumah Sakit Dr. Hasan Sadikin Bandung pada Januari 2016 yang menunjukkan segmen dari kromosom 13 translokasi ke kromosom 18 atau 47,XY,+13,t(13:18).
Correlation between Serum Ferritin, Serum Cystatin C, and Renal Function in Children with β Thalassemia Major Permadi, Suci Saptyuni; Renarti, Lelani; Rachmadi, Dedi
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1666

Abstract

Renal dysfunction caused by iron overload is characterized by an increase in ferritin and cystatin C levels. The objective of this study was to determine the correlation between ferritin, cystatin C, and renal function in children with β thalassemia major. A cross-sectional observational analytic study was conducted in September 2018 on 34 children with β thalassemia major in Dr. Hasan Sadikin General Hospital. Ferritin and cystatin C levels were documented and the estimated glomerular filtration rate (eGFR) was calculated using the Schwartz formula. Statistical tests were performed using Rank Spearman and Point Biserial with p value of <0.05 considered significant. the median ferritin level, cystatin C level, and eGFR of the subjects were 2,818 ng/mL (95% CI: 2,505–3,977), 209.9±121.5 (95% CI: 167.5–252.3), and 185.5 mL/min /1.73 m2 (95% CI: 173.6–208.2), respectively. Correlations were sought between serum ferritin and eGFR (r=0.132, p=0.229), between cystatin C and eGFR: r=0.3012, p=0.041, and between ferritin and cystatin C: r=0.433, p=0.011. No correlation was found between ferritin and renal function whereas serum cystatin C presented a positive correlation with renal function. A strong correlation was found between ferritin and cystatin C. Serum ferritin and cystatin C are promising biomarkers to assist in monitoring renal function in children with β thalasemia major.Hubungan Feritin dan Cystatin C Serum dengan Fungsi Ginjal pada Anak Talasemia β Mayor Kelebihan besi pada anak talasemia β mayor mengganggu organ vital di antaranya ginjal. Gangguan fungsi ginjal karena kelebihan besi dapat ditandai dengan peningkatan kadar ferritin dan cystatin C. Penelitian ini bertujuan mengetahui hubungan kadar feritin dan cystatin C dengan fungsi ginjal pada anak talasemia β mayor. Penelitian observasional analitik dengan rancangan potong lintang, dilaksanakan bulan September 2018. Subjek penelitian adalah anak penderita talasemia β mayor di Rumah Sakit Dr. Hasan Sadikin Bandung. Dilakukan pemeriksaaan kadar feritin dan cystatin C. Penilaian fungsi ginjal menggunakan estimated glomerular filtration rate (eGFR) dengan formula Schwartz. Uji statistik menggunakan uji Rank Spearman dan Point Biserial dengan kemaknaan berdasar nilai p<0,05. Didapatkan jumlah sampel sebanyak 34 anak, dengan kadar feritin median 2818 ng/mL (IK95%:2505–3977), cystatin C 209,9±121,5 (IK95%:167,5–252,3) dan eGFR median 185,5 mL/menit per 1,73 m2(IK95%:173,6–208,2). Korelasi antara kadarferritin dengan eGFR: r=0,132, p=0,229, korelasi cystatin C dengan eGFR: r=0,3012; p=0,041 dan korelasi kadar feritin dengan cystatin C: r=0,433; p=0,011). Hasil penelitian ini tidak didapatkan korelasi antara feritin dan fungsi ginjal, sedangkan cystatin C memiliki korelasi positif dengan fungsi ginjal. Didapatkan korelasi kuat antara kadar feritin dan cystatin C serum. Pemeriksan kadar feritin dan cystatin C dapat membantu pemantauan fungsi ginjal pada anak talasemia β mayor.
Histopathological Effects of Ageratum Leaf Extract (Ageratum Conyzoides) on Wound Healing Acceleration After Acute Excisional Wound on Epidermis in Type 2 Diabetes Mellitus Model of Sprague Dawley Rats (Rattus norvegicus) Yudhantoro, Liliek; Hidajat, Nucki Nursjamsi; Ismiarto, Yoyos Dias; Ismono, Darmadji
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1652

Abstract

Diabetic wound healing problem often occurs if proper care is not given which will lead to the development of a chronic wound. Ageratum (Ageratum conyzoides L.) leaves is one of the most common plants in tropical areas, including Indonesia, which are frequently used in traditional treatment due to its anti-bacterial properties. This experimental study aimed to identify the effects of topical application of Ageratum leaf extract on wound healing based on histopathological examination of the reepithelization, ulceration, neovascularization, and the presence of inflammatory cells. This study was performed in the period of September to December 2017 at the Pharmacology and Pathology Anatomy laboratories of Dr. Hasan Sadikin General Hospital Bandung, Indonesia. Excisions were made on the back of the 36 rats that were divided into control and test groups. The test group then received a topical application of Ageratum leaf extract. The resulting histopathologic appearance of the wound was then examined. The test group showed better wound healing in all parameters inspected when compared to the control group. Mann-Whitney Test with 95% confidence interval (p<0.05) showed that the re-epithelialization, ulceration, neovascularization, and presence of inflammatory cells reflected a statistically significant improvement in the test group (p=0.319, p=0.290, p=0.251, and p=0.245, respectively). This study concludes that the topical application of Ageratum leaf extract has a statistically significant benefit on diabetic wound healing.Pengaruh Histopatologis Ekstrak Daun Ageratum (Ageratum Conyzoides) terhadap Akselerasi Penyembuhan Luka Setelah Luka Eksisi Akut pada Epidermis pada Model 2 Diabetes Mellitus Tikus Sprague Dawley (Rattus norvegicus) Luka diabetes sering menyebabkan luka kronik jika tidak mendapat penanganan yang tepat. Ageratum (Ageratum conyzoides) merupakan tanaman khas daerah tropis termasuk Indonesia, yang daunnya digunakan untuk pengobatan tradisional karena mengandung zat anti bakteri. Penelitian eksperimental ini menggunakan desain kelompok kontrol post-test untuk mengidentifikasi efek aplikasi topical dari ekstrak daun Ageratum pada penyembuhan luka dengan pemeriksaan histopatologis dari reepitelialisasi, ulserasi, neovaskularisasi, dan sel radang. Penelitian ini dilakukan antara September hingga Desember 2017 di laboratorium Farmakologi dan Patologi Anatomi Rumah Sakit Umum Dr. Hasan Sadikin Bandung. Eksisi dilakukan pada tiap-tiap tikus dari 36 tikus yang dibagi menjadi kelompok kontrol dan kelompok uji di mana kelompok uji dilakukan perawatan dengan ekstrak etanol daun ageratum secara topikal. Setelah diaplikasikan ekstrak daun Ageratum, luka diperiksa secara histopatologi. Observasi pada kelompok uji menunjukkan penyembuhan luka eksisional tikus diabetes yang lebih baik daripada kelompok control untuk semua parameter. Uji Mann-Whitney dengan interval kepercayaan 95% (p <0,05) menunjukkan bahwa nilai p untuk reepitelisasi, ulserasi, neovaskularisasi, dan sel radang adalah p=0,319, p=0,290, p=0,251, dan p=0,245. Aplikasi topikal dari ekstrak daun Ageratum memiliki manfaat pada penyembuhan luka diabetes dan hasilnya signifikan secara statistik. 

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