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Articles 16 Documents
Search results for , issue "Vol 34, No 02 (2002)" : 16 Documents clear
Nutritional status, growth, dietary intake in asthmatic children: a study to Junior High Schools in Yogyakarta municipality. Elisa, Elisa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Objective: To know the relationship between asthma and nutritional status, growth, and dietary intake. Design: Prospective cohort study of children aged 12-18 years.Setting: Twenty eight Junior High Schools in Yogyakarta municipality.Subjects: There were 802 children who ever had symptoms of asthma and children who never had asthma as control. Questionnaire was used to establish the diagnosis of asthma. Subjects were grouped as: 1) ever had symptom of asthma but not in the last 6 months; 2) ever had 1 symptom of asthma in the last 6 months; 3) ever had 2 symptoms of asthma in the last 6 months; 4) ever had 3 symptoms of asthma in the last 6 months; and 5) never had asthma.Main outcome measures: Z-score for weight for age and height for age, body mass index (BMI), and dietary intake.Results: This study revealed there was no statistical difference between asthmatic group and control group in nutritional status based on weight for age in the first measurement (p=0.292), the second (p=0.258), and the third (p=0.122); based on height for age, in the first measurement (p=0.356), the second (p=0.541), the third (p=0.384). Likewise, based on body mass index, in the first measurement (p=0.335), the second (p=0.305), the third (p=0.166). Concerning growth there was no statistical difference between asthmatic group and control group based on weight for age from the first to the second measurement (p=0.215), second to third Ip=0.866), first to third (p =0.188); based on height for age from the first to the second measurement Ip=0.774), second to third (3=0.627), first to third (p=0.738. Likewise, based on body mass index from the first to the second measurement (p=0.556), second to third (p=0.913), first to third (p=0.784). Daily intake of nutrition revealed there was no statistical difference between asthmatic group and control group concerning calorie (p=0.075), protein (p=0.345), fat (p=0.989), carbohydrate (p=0.731), calsium (p=0.936), iron (p=0.566), vitamin A (p=0.457), vitamin B (p=0.932, and vitamin C (p=0.837).Conclusion: Asthma did not affect nutritional status, growth, and dietary intake. There was no significant difference on growth among study groups.Key words: asthma - nutritional status - growth - dietary intake
Behavior of the B. malayi microfilariae in the peripheral blood of human carrier in the intergradation area of Mahakam Delta East Kalimantan. FA Sudjadi, FA Sudjadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Nonperiodic form of B. malayi of human parasite was recently identified in East Kalimantan by Sudjadi as different subspecies in addition to the previously known periodic and subperiodic form of infraspecific variation.Objectives: To detect any changes in microfilarial periodicity in the intergradation area of Mahakam delta with the subperiodic type from South Sulawesi, genealogically related to local population. Methods: Periodicity examinations were carried out on the microfilarial carriers discovered in the B. malayi endemic area of Mahakam delta. Blood examinations (60 mm3 blood) was carried out every 2 hours, in 24 hour cycles. Result analysis was performed using Aikat and Das statistical formula. Results: Six microfilaria carriers of B. malayi were examined for periodicity study. Cases of the nonharmonic or noncircadian form were predominantly (in 5 cases) recorded and only in one case the microfilariae were showed a harmonic or circadian wave pattern,, but with a peak hour at day time, ie 15.5400". Conclusion: No change of the microfilarial periodicity was recorded in the intergradation zone of Mahakam delta. The B. malayi filarial worm was still showing a nonperiodic wave pattern as different subspecies apart from the subperiodic and periodic form of filarial worm.Key words: B. malayi - microfilarial periodicity - intergradation area - non periodic form - subperiodic form
Random amplified polymorphic DNA (RaPD) markers readily distinguish crytic mosquito species(diptera:culicidae) Budi Mulyaningsih, Budi Mulyaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

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The relationship of asthma with physical fitness of Junior High School students in Yogyakarta. Isri Muninggar, Isri Muninggar
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Background: The understanding on asthma and the patients daily quality of life remain an issue which still need to be elucidated. Information about the relationship between asthma and physical fitness is an important factor that has to be considered in the management of asthma patients.Objective: To examine the relationship between asthma and the physical activity and physical fitness, with regards to the factors influencing them, in junior high students in Yogyakarta.Research design: crosss sectional.Materials and Methods: The samples were part of a main research project on the prevalence of asthma in the population of junior high school students in Yogyakarta. The incidence of asthma was determined using a previously validated modified questionnaire from ISAAC, ATS 78 and Robertson. Cases were students diagnosed as asthma, and the controls were students without asthma. Cases and controls were matched for age, sex, and school year. The basic characteristics of the students and parents, daily circle activities, history of smoking, school grade and physical exercise, the time needed to run a 100 meters distance were obtained using a quesionnaire. Lung function tests were done before and after 10 minutes static bicycle exercise. Data are analyzed using student T-test and X2 test.Results: There were 219 students who fulfilled the inclusion criteria, consisted of 102 males (46.57%) and 117 females (53.43%). The case group (asthma) comprised 119 students while 100 students were the control group. There is no statistically significant difference between the average age of subjects in the case group (14.70 years) with the control group (14.62 years) (p=0.416). There were no statistically significant differences between the cases and controls concerning the school grade and physical exercise (p=0.243), the time needed to run 100 meters distance (p=0.959), daily activities, physical activities: athletic (p=0.511), game playing sport (p =0.342), bicycling (p =0.212), swimming (p=0.586), the ability to joint to physical activities/physical exercise. We also could not find statistically significant differences in the lung function test before: VC p=0.271, FVC p =0.694, FEV1 p=0.995, FEV1/FEC p=0.579, PEF 25% p=0.144, PEF 25-75% p=0.179, MVV p=0.340 and Ex. Time p=0.100, and after exercise using static bicycle for 10 minutes VC p=0.347, FVC p=0.805, FEV1 p=0.767, FEV1/FEC p=0.401, PEF 25% p=0.152, PEF 25-75% p=0.210, MVV p =0.310.Conclusion: There is no statistically significant difference in daily physical activities and school physical exercise activity between students in the asthmatic group and control group.Key words: Asthma physical fitness - physical activity - incidence of smoking - nutritional status - growth and development - lung function test
Amplification and cloning of block 2 of the gene encoding Merozoite Surface Protein-1 (MSP-1) of Plasmodium falciparum isolated from Kokap, Yogyakarta Elsa Herdiana, Elsa Herdiana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Falciparum malaria remains a major public health problem in tropical and subtropical countries including Indonesia. The disease is caused by Plasmodium falciparum and spread by Anopheline mosquitoes. The widespread of Plasmodium which are resistant to antimalarial drugs and Anopheline mosquitoes which are resistant to insecticides have urged to look for alternative solutions including the development of protective vaccines. Several vaccine candidates have been studied, one of them is Merozoite Surface Protein-1 (MSP-1) which is expressed on the surface of merozoite. It was shown that this protein induces protective immune responses. Variation on the gene encoding MSP-1 of Plasmodium falciparum has been well documented but such data from Indonesia population have never been studied. Objective: The aim of this study is to amplify and clone MSP-1 gene of P. falciparum isolated from Kokap, Yogyakarta.Methods: Block 2 of the gene encoding MSP-1 was amplified by Polymerase Chain Reaction (PCR) and the PCR amplification products were cloned using pGEM® -T vector and transformed into Escherichia coil JM 109.Result: From 19 PCR results, 3 were cloned and 10 colonies were picked up. Nine of 10 showed the MSP-1 gene insertion by PCR method.Conclusion: Block 2 of the gene encoding MSP-1 of P. falciparum isolated from Kokap, Yogyakarta was successfully amplified by PCR method. This study resulted in 9 recombinant plasmids which contained MSP-1 gene as the outcome of cloning and transformation into E.coli.Key words: P. falciparum - MSP-1 - PCR - Amplification - Cloning
The detection of one-roof contact individuals on the tuberculosis infection of primary school students in Yogyakarta Municipality Sri Wijayamti, Sri Wijayamti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: With the detection of tuberculosis (TB) infection in children means that there are transmitting infection by the lung TB carrier with positive acid fast (AF) sputum. The transmission more frequently occurred in one-roof close contact.Objective: To know the relationship between the TB infection in primary school students and the one-roof family member with positive AF.Material and methods: This study was performed from May 1997 to September 1999 using case-control study design. The subjects of the study were primary school students in Yogyakarta Municipality. The primary schools involved were selected using proportional population random sampling method by the area IPDA. The study was performed by active case finding of one-roof family members with over 2 week successive cough and performing the sputum culture. As the controls were the students who were confirmed not infected by TB after BCG test, matched for their age and sex. The statistical analysis using univariable and the clinical significance of the risk factor was inferred from the confidence interval.Result: One hundred and forty one TB-infected students were assigned as the cases and 189 students not infected by TB were assigned as the controls. The one-roof family members with over 2 week successive cough were found more in the case group than in thecontrol group, with the Odds ratio = 2.83; (95% CI = 1.01-5.611. The one-roof family members with over 2 week successive cough and positive AF sputum were found only 1 person in each group, with the Odds ratio = 1.34; (95% CI = 0.08-21.65); X2 =. 0.04; p = 0.84.Conclusion: There were more one-roof family members with over 2 week successive cough in the case group. The presence of a one-roof family member with over 2 week successive cough and positive AF sputum culture, was not proved statistically and not conclusive clinically but tendency to be conclusive as the risk factor of the incidence of TB infection in children.Keywords: TB in children - BCG test - risk factor - contact person - positive sputum culture
Wary tuberculosis and cholestatic type hepatitis. A case report Edi Hartoyo, Edi Hartoyo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Universitas Gadjah Mada

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Abstract

Tuberculosis is still a major health problem in Indonesia. The organ commonly affected is lung (pulmonary tuberculosis) but other organs (extrapulmonary tuberculosis) may also be involved. Working diagnosis of tuberculosis in childhood is usually established by tuberculin test, chest x-ray and clinical manifestations, but it is often difficult to establish diagnosis of childhood tuberculosis as the chest x-ray and clinical manifestations are not always specific and we have difficulty in finding tuberculosis such as scoring method, bactec, serologic examination and biomolecular technic. However all of those examinations require further study for practical clinical use in the future. We report 12 year old child suffering from miliary tuberculosis and cholestatic type hepatitis. The recommended treatment for miliary tuberculosis is INH 10 mg/kgbw/day, Rifampicin 15 mg/kgbw/day, Ethambutol 15 mg/kgbw/day, Pyrazinamid 20 mg/ kgbw/day (for 2 months). Thereafter, the therapy was continued according to the conventional protocol. For the cholestatic type hepatitis we gave ursodeoxycolic (urdafalk) 8 mg/kgbw/day and cursil 2 x 1. The patients condition improved after evaluation of 3 week therapy.Key words: miliary tuberculosis - diagnostic problem - cholestatic hepatitis - management
Pro-inflammatory cytokine level and neutrophil count in acute ischemic stroke Suroto, Suroto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Pro-inflammatory cytokines are cytokines which are often found to be increased in an inflammatory process. In acute ischemic stroke there is an inflammatory response which affects the progress of the stroke.Objectives: The aim of this study was to understand the correlation between pro-inflammatory cytokines and neutrophil count in the peripheral blood of acute ischemic stroke patients.Methods: Peripheral blood was taken from 30 acute ischemic stroke patients age 50-70 years old on the third day of stroke attack. Leukocytes and differential count were examined, also level of TNF alpha, IL-1 and IL-8. Confirmation of ischemic stroke was determined by the head CT scan without contrast media on the fourth day of stroke attack. Thirty subjects without stroke were taken as control, with matching in age, gender and ethnic group.Results: The result showed a significant difference in neutrophil count between stroke group and control group (F 0.001). There was also significant difference among the 3 pro-inflammatory cytokines in 2 groups (F for TNF alpha: 0.007, IL-1: 0.055 and IL-8: 0.002). In bivariate correlation test, there was a significant correlation between IL-8 level and neutrophil count in the blood (p 0.0000).Conclusion: It is concluded that pro-inflammatory cytokines especially IL-8 has a role in recruiting neutrophil as an important component in the inflammatory response in acute ischemic stroke.Keywords: ischemic stroke - pro-inflammatory cytokines - IL-8 - neutrophil - correlation
Wary tuberculosis and cholestatic type hepatitis. A case report Edi Hartoyo, Edi Hartoyo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

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Abstract

Tuberculosis is still a major health problem in Indonesia. The organ commonly affected is lung (pulmonary tuberculosis) but other organs (extrapulmonary tuberculosis) may also be involved. Working diagnosis of tuberculosis in childhood is usually established by tuberculin test, chest x-ray and clinical manifestations, but it is often difficult to establish diagnosis of childhood tuberculosis as the chest x-ray and clinical manifestations are not always specific and we have difficulty in finding tuberculosis such as scoring method, bactec, serologic examination and biomolecular technic. However all of those examinations require further study for practical clinical use in the future. We report 12 year old child suffering from miliary tuberculosis and cholestatic type hepatitis. The recommended treatment for miliary tuberculosis is INH 10 mg/kgbw/day, Rifampicin 15 mg/kgbw/day, Ethambutol 15 mg/kgbw/day, Pyrazinamid 20 mg/ kgbw/day (for 2 months). Thereafter, the therapy was continued according to the conventional protocol. For the cholestatic type hepatitis we gave ursodeoxycolic (urdafalk) 8 mg/kgbw/day and cursil 2 x 1. The patient's condition improved after evaluation of 3 week therapy.Key words: miliary tuberculosis - diagnostic problem - cholestatic hepatitis - management
The relationship of asthma with physical fitness of Junior High School students in Yogyakarta. Isri Muninggar, Isri Muninggar
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 02 (2002)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

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Abstract

Background: The understanding on asthma and the patient's daily quality of life remain an issue which still need to be elucidated. Information about the relationship between asthma and physical fitness is an important factor that has to be considered in the management of asthma patients.Objective: To examine the relationship between asthma and the physical activity and physical fitness, with regards to the factors influencing them, in junior high students in Yogyakarta.Research design: crosss sectional.Materials and Methods: The samples were part of a main research project on the prevalence of asthma in the population of junior high school students in Yogyakarta. The incidence of asthma was determined using a previously validated modified questionnaire from ISAAC, ATS 78 and Robertson. Cases were students diagnosed as asthma, and the controls were students without asthma. Cases and controls were matched for age, sex, and school year. The basic characteristics of the students and parents, daily circle activities, history of smoking, school grade and physical exercise, the time needed to run a 100 meters distance were obtained using a quesionnaire. Lung function tests were done before and after 10 minutes static bicycle exercise. Data are analyzed using student T-test and X2 test.Results: There were 219 students who fulfilled the inclusion criteria, consisted of 102 males (46.57%) and 117 females (53.43%). The case group (asthma) comprised 119 students while 100 students were the control group. There is no statistically significant difference between the average age of subjects in the case group (14.70 years) with the control group (14.62 years) (p=0.416). There were no statistically significant differences between the cases and controls concerning the school grade and physical exercise (p=0.243), the time needed to run 100 meters distance (p=0.959), daily activities, physical activities: athletic (p=0.511), game playing sport (p =0.342), bicycling (p =0.212), swimming (p=0.586), the ability to joint to physical activities/physical exercise. We also could not find statistically significant differences in the lung function test before: VC p=0.271, FVC p =0.694, FEV1 p=0.995, FEV1/FEC p=0.579, PEF 25% p=0.144, PEF 25-75% p=0.179, MVV p=0.340 and Ex. Time p=0.100, and after exercise using static bicycle for 10 minutes VC p=0.347, FVC p=0.805, FEV1 p=0.767, FEV1/FEC p=0.401, PEF 25% p=0.152, PEF 25-75% p=0.210, MVV p =0.310.Conclusion: There is no statistically significant difference in daily physical activities and school physical exercise activity between students in the asthmatic group and control group.Key words: Asthma physical fitness - physical activity - incidence of smoking - nutritional status - growth and development - lung function test

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