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Articles 14 Documents
Search results for , issue "Vol 36, No 2 (2004)" : 14 Documents clear
Paroxysmal nocturnal haemoglobinuria with dysphagia. A case report Riadi Wirawan, Riadi Wirawan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

We reported a case of a 32 year old male with diagnosis thalassaemia and dysphagia who had been hospitalized four times at two different private hospitals. The chief complaints were weakness, brownish red discoloration of urine at night until morning and dysphagia. Physical examination revealed hepatosplenomegaly. The laboratory findings were normocytic normochromic anaemia, pancytopenia, reticulocytosis, hyperactive erythropoiesis, decreased NAP score, positive Hams test and sugar water test. These findings were in accordance with intravascular haemolytic anaemia caused by PNH. Dysphagia in this case is one of clinical manifestation of PNH. We also found disorder of liver function test such as increased transaminase activity and gGT which may be caused by cholestasis, which should be confirmed by USG.Key words : intravascular haemolytic anaemia - dysphagia - haemoglobinuria - cholestatis - pancytolemia
Variation in susceptibility status to organophosphate insecticide among several geographic populations of Aedes albopictus Skuse in Indonesia Budi Mulyaningsih, Budi Mulyaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Aedes albopictus skuse is an important vector of dengue fever and dengue hemorrhagic fever in Southeast Asia. Its distribution in Indonesia extends from Sabang (Sumatera) to Merauke (Papua) and is currently expanding. In response to a potential dengue fever outbreak in Indonesia during 2002, the susceptibility of 4 geographic populations of Aedes albopictus Skuse to commonly used mosquito adulticide (malathion) and larvicide (temephos) were assessed.Objective: The aim of this study is to determine the susceptibility status of each Ae. albopictus population to malathion and temephos.Methods: Eggs collected from 4 different geographic area in Indonesia, such as Padang (Sumatera), Yogyakarta (Java), Banjar (Kalimantan) and Timika (Papua) were reared to larvae and adults and were investigated by using bioassay (World Health Organization, 1981). The bioassay data were analyses using Probit analysis, which also corrects for mortality in the controls by Abott formula.Result: The Ae. albopictus mosquito population originate from Yogyakarta shows highest LC50 and LC90 malathion and temephos value, and the Ae. albopictus mosquito population originate from Timika shows lowest LC50 and LC90 malathion and temephos value than other population.Conclusion: There were insecticide susceptibility status difference of Ae. albopictus from diffetent geographic population in Indonesia (Padang, Yogyakarta, Banjar and Timika).Key words: malathion – temephos - Ae. albopictus - bioassay - Abott formula.
Malondialdehyde level in the lens of complicated cataract patients with the instillation of diclofenac sodium 0,1% eye drop pre-operatively Wiwik Widowati, Wiwik Widowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Universitas Gadjah Mada

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Abstract

Objective: To determine the level of malonaldehyde of lens in complicated cataract patients receiving instillation of diclofenac sodium 0,1% eye drop, 3 times daily, 2 weeks before undergoing surgery. Materials and methods: A cross sectional study on complicated cataract patients who underwent extracapsular cataract extraction, was conducted at Dr. Sardjito General Hospital, dr. Yap Eye Hospital and mass cataract surgery in Yogyakarta and surrounding area, from November 2002 until November 2003. Subjects were consecutively recruited and classified into two groups. Twenty patients received diclofenac sodium 0,1% eye drop 3 times daily for two weeks before lens extraction, and 20 patients who received no treatment served as control. On the day of surgery, the lens was taken to measure the level of MDA at the Laboratory of Biochemistry. The difference of MDA level between the two groups was statistically analyzed by student t test.Results: The mean MDA level in the group receiving diclofenac sodium was 4,386 ± 2,625 nmol/g, while in the group without diclofenac sodium it was 6,728 ± 3,422 nmol/g. This difference was statistically significant with p = 0.020.Conclusion: The mean MDA level in diclofenac sodium group was lower than that without diclofenac sodium group.Key words: malondialdehyde level - caractogenesis - complicated cataract - diclofenac sodium.
Anticancer selectivity of Momordica charantia L. Seeds Extract and Column Fraction on Raji , HeLa and T47D Cell Lines Hersri Rochmad Parhardian, Hersri Rochmad Parhardian
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Momordica charantia L. has been used traditionally as medicinal plant in Indonesia. The seed extracts have active substances in the form of ribosome-inactivating proteins (RIPs. Almost all RIPs can eliminate virus infection and kill cancer cells. Some cancer cells like Raji cell line and HeLa cell line are derived from Burkitts lymphoma and cervical carcinoma, respectively. Both cancers shows a striking association with Epstein-Barr Virus (EBV) and human papilloma virus (HPV).T47D cell line is derived from breast cancer and is not related to certain type of virus.Objective: This study was focused on the effect of RIP from M.charantia L. on Raji, HeLa and T47D cell lines.Methods: Crude extract from M.charantia L. seeds was dissolved in 0.15 M NaCI, and then precipitated with 30-60% saturated ammonium sulphate. Precipitant was then purified with column of CM-Sepharose CL-6B with 0.5 M NaCI gradient. Cell lines grown in growth media with crude extract and active fraction of column. Lethal Doses 50% (LD50) was calculated directly for Raji cell line and used MTT for HeLa and T47D cell lines.Results: The study shows that 0.25M NaCI column fraction containing 32kDa protein had an activity on DNA supercoiled cleavage. The difference of LD50 between crude extract with 32kDa protein was not significant (p>0.05) for Raji cell line and HeLa cell line, but there was significant difference for T47D cell line. T47D cell line shows DNA fragmentation as sign of apoptosis.Conclusion: Raji cells shows more sensitive than T47D cell lines did, whereas HeLa cell line was resistant to the crude extract and 32kDa protein. In this work, the death of T47D cell line was estimated through apoptosis.Key words: column fraction - Momordica charantia L. - Raji - T47D cell lines - apoptosis.
Drugs availability at districs level and the quality of prescribing in primary health care Iwan Dwiprahasto, Iwan Dwiprahasto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Universitas Gadjah Mada

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Abstract

Background: An excessive use of drugs in primary health care is commonly unavoidable. This in part due to the limited knowledge of prescribers who are mostly paramedics and whose pharmacotherapeutic knowldges are limited. More than 90% patients with acute respiratory infection (ARI) and diarrhea received antibiotics and similar percentage of patients with muscle ache received injection unecessarily. Objective: To improve the availability of drugs at district level and to improve the quality of prescribing at primary health centers (PHCs).Design: Quasi experimental with pre and post test analysis and using a control group for comparison. An interactive, problem-based training intervention was carried out within 2 days period involving all PHCs and districts warehouses in three provinces, i.e West Kalimantan, West Sumater, and West Nusa Tenggara. Monitoring, supervision and feedback were carried out regularly upon completion of the inter- vention. This study involves 18 districts and 118 PHCs.Results: 11 .995 prescriptions were collected at baseline, 6 months and 1 year after intervention. An average number of drugs per prescription decreased significantly 1 year after intervention (p<0.05), while no improvement was found in the control group. The use of antibiotics for ARI decreased consis- tently and significantly 6 months and 1 year after intervention (p<0.05). The use of injection for muscle ache also significantly decreased 1 year after intervention (p<0.05). Drug availability at district level could be maintained at an ideal level (1 2-18 months) and no stock out was detected 1 year after the intervention.Conclusions: Interactive, problem-based training on rational use of drugs and training on managing drug supply could improve the quality of prescribing and drug availability at district level.Key words: drug availability - stock out - problem-based training - acute respiratory infection - diarrhea 
Cloning and expression of cDNA encoding 28 kDa soluble protein (GRA2) tachyzoite of Toxoplasma gondii local isolate Jarot Subandono, Jarot Subandono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Toxoplasma gondii is an intracellular parasite that causes toxoplasmosis and found in tropical countries. Toxoplasmosis is dangerous if suffered by pregnant woman or immunodeficiency patients. T. gondii has 28 kDa soluble protein from dense granule (GRA2) and among GRA proteins, GRA2 is probably the most immunogenic. Disruption of the GRA2 locus in T. gondii has resulted in decreasing of the parasite virulence in mice. Invasion of tachyzoite T. gondii into macrophages was also significantly inhibited by anti GRA2 antibody, therefore the availability of GRA2 protein is essential for development of protective vaccine.Objective: The aim of this research was to produce 28 kDa soluble protein (GRA2) by cloning and expression of cDNA encoding soluble protein tachyzoite of T. gondii local isolate.Methods: Total ribonucleic acid (RNA) and messenger RNA was isolated from tachyzoite of local T. gondii grown up in Balb/c mice. Messenger RNA was isolated from total RNA using polyATtract mRNA Isolation Systems and synthesis of cDNA using Universal RiboClone cDNA Synthesis Systems. Recombinants of pUC18 were transformed into E. co/i XL1-Blue by heat shock technique. Expression of recombinant protein was analysed by immunoblotting using polyclonal antibodies against soluble protein of T. gondii. Results: Two recombinant clones were isolated which expressed 28 kDa recombinant protein.Conclusion: Two recombinant clones were isolated. The immunoblotting result indicates that the recombinant expressed 28 kDa recombinant protein which hybridized with the antibody polyclonal against soluble protein of T. gondii and the proteins are possibly GRA2 proteins.Key words : Toxoplasma gondii - tachyzoite - cDNA - 28 kDa soluble protein.
Study of medical report on justice examination result at Dr. Sardjito Hospital Beta Ahlam Gizela, Beta Ahlam Gizela
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Every human violation or crime case, in which there is human as a victim needs medical proofing, namely the Medical Report for justice or Visum et Repertum in Indonesia. We also have an obligation in keeping medical confidentiality in medical practice. It is therefore, necessary to study relevance regulation of examination and disclosing medical confidentiality for justice.Objectives: The purpose of this study is to analyse management of Visum et Repertum of life person at Sardjito Hospital Yogyakarta.Methods: Visum et Repertum of life person at Medical Record Department of Sardjito Hospital was reviewed. The MEG protocol for examination and reporting from International Amnesty used as a standard. Results: The study shows that management of Visum et Repertum of life person at Sardjito Hospital was administratively inappropiate. There were some discrepancies between regulation and practice. There is no agreement between each doctors in managing cases. Generally, Visum et Repertum was accepted in content, but for the future an improvement is needed as International Amnesty.Conclusions: Management of life person cases for Visum et Repertum at Sardjito Hospital do not international standard.Key words: visum et repertum - living person - medical confidentiality - informed consent
Paroxysmal nocturnal haemoglobinuria with dysphagia. A case report Riadi Wirawan, Riadi Wirawan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.386 KB)

Abstract

We reported a case of a 32 year old male with diagnosis thalassaemia and dysphagia who had been hospitalized four times at two different private hospitals. The chief complaints were weakness, brownish red discoloration of urine at night until morning and dysphagia. Physical examination revealed hepatosplenomegaly. The laboratory findings were normocytic normochromic anaemia, pancytopenia, reticulocytosis, hyperactive erythropoiesis, decreased NAP score, positive Ham's test and sugar water test. These findings were in accordance with intravascular haemolytic anaemia caused by PNH. Dysphagia in this case is one of clinical manifestation of PNH. We also found disorder of liver function test such as increased transaminase activity and gGT which may be caused by cholestasis, which should be confirmed by USG.Key words : intravascular haemolytic anaemia - dysphagia - haemoglobinuria - cholestatis - pancytolemia
Variation in susceptibility status to organophosphate insecticide among several geographic populations of Aedes albopictus Skuse in Indonesia Budi Mulyaningsih, Budi Mulyaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.839 KB)

Abstract

Background: Aedes albopictus skuse is an important vector of dengue fever and dengue hemorrhagic fever in Southeast Asia. Its distribution in Indonesia extends from Sabang (Sumatera) to Merauke (Papua) and is currently expanding. In response to a potential dengue fever outbreak in Indonesia during 2002, the susceptibility of 4 geographic populations of Aedes albopictus Skuse to commonly used mosquito adulticide (malathion) and larvicide (temephos) were assessed.Objective: The aim of this study is to determine the susceptibility status of each Ae. albopictus population to malathion and temephos.Methods: Eggs collected from 4 different geographic area in Indonesia, such as Padang (Sumatera), Yogyakarta (Java), Banjar (Kalimantan) and Timika (Papua) were reared to larvae and adults and were investigated by using bioassay (World Health Organization, 1981). The bioassay data were analyses using Probit analysis, which also corrects for mortality in the controls by Abott formula.Result: The Ae. albopictus mosquito population originate from Yogyakarta shows highest LC50 and LC90 malathion and temephos value, and the Ae. albopictus mosquito population originate from Timika shows lowest LC50 and LC90 malathion and temephos value than other population.Conclusion: There were insecticide susceptibility status difference of Ae. albopictus from diffetent geographic population in Indonesia (Padang, Yogyakarta, Banjar and Timika).Key words: malathion – temephos - Ae. albopictus - bioassay - Abott formula.
Malondialdehyde level in the lens of complicated cataract patients with the instillation of diclofenac sodium 0,1% eye drop pre-operatively Wiwik Widowati, Wiwik Widowati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.959 KB)

Abstract

Objective: To determine the level of malonaldehyde of lens in complicated cataract patients receiving instillation of diclofenac sodium 0,1% eye drop, 3 times daily, 2 weeks before undergoing surgery. Materials and methods: A cross sectional study on complicated cataract patients who underwent extracapsular cataract extraction, was conducted at Dr. Sardjito General Hospital, dr. Yap Eye Hospital and mass cataract surgery in Yogyakarta and surrounding area, from November 2002 until November 2003. Subjects were consecutively recruited and classified into two groups. Twenty patients received diclofenac sodium 0,1% eye drop 3 times daily for two weeks before lens extraction, and 20 patients who received no treatment served as control. On the day of surgery, the lens was taken to measure the level of MDA at the Laboratory of Biochemistry. The difference of MDA level between the two groups was statistically analyzed by student t test.Results: The mean MDA level in the group receiving diclofenac sodium was 4,386 ± 2,625 nmol/g, while in the group without diclofenac sodium it was 6,728 ± 3,422 nmol/g. This difference was statistically significant with p = 0.020.Conclusion: The mean MDA level in diclofenac sodium group was lower than that without diclofenac sodium group.Key words: malondialdehyde level - caractogenesis - complicated cataract - diclofenac sodium.

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