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Articles 18 Documents
Search results for , issue "Vol 39, No 01 (2007)" : 18 Documents clear
The effect of fructooligosaccharide (FOS) in Children With Diarrhea Yati Soenarto, Mohammad Juffrie Yunri Istari, Nenny Sri Mulyani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Latar belakang: Penyakit diare masih merupakan penyebab penting kesakitan dan kematian bayi dan anak di negara berkembang. Penyakit diare ini disebabkan oleh ketidakseimbangan flora usus yang dipengaruhi oleh jumlah bakteria yang menguntungkan . FOS dapat menstimulasi pertumbuhan bakteri yang menguntungkna dan menghambat bakteri pastogen yang menyebabkan infeksi saluran cerna Tujuan: Mengetahui efekFructooligosaccharide pada diareBahan dan cara: penelitina kendali acak dilakukan di 8 puskesmas di Yogyakarta. Seratus delapan belas sampel ikut dalam penelitian ini, 93 masuk dalam kelompok FOS yang mendapat FOS 2,5 gram atau 5 gram per hari sesuai umur, dan 25 masuk dalam kelompok plasebo. Lamanya diare dan keasaman tinja diukur selama penelitianHasil: Lama diare lebih pendek secara bermakna pada kelompok FOS dibanding plasebo p
Cytotoxic Activity of /V-alkyl and /V-benzyl 1,10-phenathroline derivatives in Human Cancer Cell Lines Isnatin Miladiyah, Esti Nurwening Sholikhah Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Background: In our study on the antiplasmodial activity of 1,1 O-phenanthroline derivatives, we found some compounds possessing a potential cytotoxic in normal cell line.Objective: In this study we tested these derivatives in human cancer lines in order to know their in vitro anticancer activity.Materials and methods: Six derivatives of 1,1 O-phenanthroline, 4 derivatives of N-alkyl and 2 derivatives of N-benzyl 1,1 O-phenanthroline were tested on two human cells cancer, myeloma (NS-1) and HeLa cells line. Cytotoxic activity was evaluated by trypan blue exclusion assay and their activity was expressed by the concentration inhibiting 50% of the cell growth (lCso) The ICso of each compound was determined by probit analysis.Results: The results showed that the ICso values of 1,1 O-phenanthroline derivatives ranged from 4.68 to 15.63 pM on myeloma cell and from 2.82 to 16.89 pM on HeLa cell. The 4-Bromo-3(2-bromoetyll-2metyl-1 , 1 O-phenantroline (3) and (1 )-N-(4-benzyloxy-3-methoxy-benzyll-1 ,1 O-phenanthrolinium chloride (6) with ICso values ranged from 4.68 to 4.72 pM on myeloma cell showed the same (p>0.05) cytotoxicity with doxorubicin, with ICso values ranged from 2.82 to 3.08 pM on HeLa cell showed the higher (p
ntiplasmodial Activity of Fractions Isolated from Methanolic Extrac of Meniran Herb (Phyllanthus niruri L) traditionally Used to Treat Malaria Subagus Wahyuono, Mustofa Esti Nurwening Sholikhah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Latar belakang: Tanaman meniran (P.niruri L.) secara tradisional telah digunakan untuk mengobati malaria. Penelitian pendahuluan terhadap berbagai ekstrak tanaman meniran menunjukkan bahwa ekstrak metanol adalah aktif terhadap P.falciparum. Namun demikian, fraksi manakah dalam ekstrak metanol yang mempunyai aktivitas antiplasmodium belum pernah dikaji.Tujuan: untuk mengetahui aktivitas antiplasmodium fraksi aktif tanaman meniran.Bahan dan cara: Fraksinasi yang mengacu pada bioassay dilakukan dengan menggunakan pelarut yang sesuai untuk memisahkan fraksi aktif dari ekstrak metanol. Pada awalnya, ekstrak metanol dipisahkan dengan etil asetat sehingga diperoleh fraksi larut etil asetat dan tidak larut etil asetat. Fraksi tidak larut etil asetat selanjutnya dipisahkan dengan kolom kromatografi dengan fase diam Sephadex LH-20 dan fase gerak n-hexan : metanol : asam asetat (7,5 : 12,5 v/v : 10 tetes) sehingga diperoleh 4 fraksi untuk diuji aktivitasnya. Aktivitas antiplasmodium pada strain P. falciparum ditunjukkan dengan nilai IC50 yaitu kadar fraksi aktif yang mampu menghambat pertumbuhan parasit hingga 50%. Identifikasi kandungan senyawa dalam fraksi aktif dilakukan dengan metode kromatografi lapis tipis (KLT).Hasil:Hasil penelitian menunjukkan bahwa fraksi tidak larut etil asetat leih aktif (IC50, 2,2-2,4 mg/mL) dari pada fraksi larut etil asetat (IC50, 4,3-4,8mg/mL). Dan pemisahan fraksi tidak larut etil asetat diperoleh 9 fraksi yang dapat dikelompokkan menjadi 4 fraksi berdasarkan gambaran KLTnya. fraksi III (FIII) merupakan fraksi paling aktif dengan IC50, 3,4-4,1 mg/mL. Identifikasi kandungan aktif F III dengan KLT menunjukkan adanya senyawa polifenol. Penelitian lanjut akan dilakukan untuk mengisolasi dan memurnikan senyawa polifenol dan mengkaji aktivitas antiplasmodiumnya.Simpulan: Fraksi III dari ekstrak metanol meniran yang mengandung senyawa polifenol merupakan fraksi paling aktif secara in vitro terhadap P.falciparum.Key words: Phyllanthus niruri, malaria, antiplasmodial activity, in vitro, polyphenol
Mutagenicity Activity of Jatropha Curcas L. (Euphorbiaceae) Latex I.Kristiani, Fazwishni Siregar
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Folklore uses of Jatropha curcas L. latex are to cure toothache, as a mouth rinse to treat bleeding gums, as a hemostatic and a wound dressing and many others. Even though the latex has been used traditionally as a plant medicament, scientific investigation including toxicological studies was very limitedObjective: The purpose of this study was to evaluate the mutagenicity of J. curcas latex by Ames method. Materials and Methods: Five increasing concentrations of diluted latex (312-5000 !-tg) were mixed with phosphate buffer or 59 mix and cultured bacteria, incubated, and then added to top agar solution. The mixture was poured into a Petri dish on top of minimal glucose agar. After incubation for 2 days, the reverted coloni were counted. If the number of revertant increases with the dose and the number are more then twice as many as that of spontaneous revertant per plate, the compound should be judged to be positive.Result: Results showed that there were no increased numbers of colonies treated with increasing concentration of J. curcas latex. This finding is observed in all bacteria used. Conclusion: J. curcas latex is not mutagenic assessed by Ames method.Key words: Jatropha curcas latex, mutagenicity test, Ames test, traditional medicament, toxicology.
Comparison of the Diagnostic Test of Leukocyte Count, Percentage of Neurtrophyl, and C-Reactive protein (CRP) in Adult Patients With Simple and Complicated Acute Appendicitis Ishandono Dachlan, Imam Sofii Agus Barmawi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: acute appendicitis is one of the acute abdomen conditions that needs an emergency surgical procedure to prevent severe complication. If the perforation is present, the complications are general peritonitis, abscess and postoperative complication such as fistula and operative wound infection. Late diagnosis can increase the mortality and morbidity. There are about 11.2%-30% cases with intestinal perforation caused by late diagnosis. The leukocyte count, percentage of neutrophyl and C-reactive protein (CRP) are valuable informations to diagnose the perforation and nonperforation acute appendicitis in adult patients.Method: a prospective cross sectional study was held on diagnostic test from patients database for acute appendicitis cases in Digestive Surgery Subdivision of Dr Sardjito Hospital from December 2005 until October 2006. The data was classified as simple and complicated acute appendicitis based on the histopathology result. Eighty two were found for both groups which fulfilled the inclusion and exclusion criteria. The diagnostic test Le. sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Likelihood Ratio (LR +), Likelihood Ratio (LR-I, accuracy and receiver operating characteristics (ROC) with area under the curve (AUC) were analyzed for the leukocyte count, neutrophyl, and C-reactive protein (CRP) in each group with histopathology result as a gold standard.Result: the leukocyte count, neutrophyl and C-reactive protein (CRP) had respectively sensitivity of: 70,07%; 74,54%; 89,09%; specificity 70,37%; 70,37%; 81,48%; Positive Predictive Value (PPV): 82,97%; 83,67%; 90,74%; Negative Predictive Value (NPV) 54,28%; 57,57%; 78,57%; Likelihood Ratio (LR+) 2,36%; 2,51 %; 4,56%, Likelihood Ratio (LR-) 0,42%; 0,22%; 0,13%, area under the curve (AUC) 0,797; 0,744; 0,891, and accuracy 70,73%; 73,17%, 86,56%.Conclusion: C-reactive protein (CRP) was a better diagnostic test to differ simple from complicated acute appendicitis in adult patient compared to leukocyte count and neutrophy!.Key words: simple and complicated acute appendicitis, leukocyte cout, neutrophyl, C- reactive protein
Sexual dimorphism of pelvic girdle: pelvimetry and pelvic types in Javanese Etty Indriati, Etty Indriati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Background: Each population has certain phenotypic traits that are unique compared to other populations. Pelviss size and morphology are two of them. A western formula of obstetric conjugate = diagonal conjugate - 1.5 to 2.0 em; may not be applicable to Javanese population; and sexual traits in Western pelves may not entirely be the same with Indonesians.Objective: to get a formula for obstetric conjugate, and conduct various pelvic measurements in order to find out the metric pattern as well as the distribution of pelvic types in Javanese.Material and Methods: 27 pelves consisting of 6 females and 21 males housed at the Department of Anatomy, Embryology and Anthropology were studied by connecting the sacrum with the right and left pelvis forming pelvic girdles. Superior inlet, diagonal conjugate, angle of greater sciatic notch, diameter and width of pelves were among the metric studied.Results: Widely used traits such as greater sciatic notch that are used in Western literature to differentiate male and female pelves in Caucasian is not consistent for Javanese pelves. The most consistent skeletal traits for sexing female pelvis are wide subpubic angle (> 75 degree), preauricular sulci, flat posterior pubic symphisis, and short ischiopubic rami. The common type of ginecoyd pelvis in Javanese (48%) is in accord with western pelvis reaching 50%. Compared to the western obstetric conjugate, Javanese obstetric conjugate has a slightly different formula = diagonal conjugate - 1 to 2.6 cm.Conclusions: Several traits in sexual dimorphic of pelvic bone in Javanese do not match with skeletal traits widely used in Western literature, portraying the importance of studying regional human biological variation. The obstetric conjugate of Javanese pelves is not entirely the same with Western formula, though the difference is not significant.Key words: Javanese, pelves, sexual dimorphism, obstetric conjugate
Body Constitution, grip stregth, and Vital Capacity of Children 15 to 18 Years in Samigaluh Highland and Galur Lowland Kulonprogo Yogyakarta Janatin Hastuti, Janatin Hastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Universitas Gadjah Mada

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Background: Growth of children at age of 15 to 18 years old has an important influence on providing body constitution in their adult. Grip strength and vital capacity express functional effort of organs or parts of the body. Therefore, it certainly relates to the shape and size of the body or the body constitution. Different environment in highland and lowland affects the body constitution and functional capacity in growth period.ObJective: The aims of this research were to know the differences of body constitution, grip strength, and vital capacity of 15-18 year old children in Samigaluh highland and Galur lowland in Kulonprogo, Yogyakarta Province, and the relationship between body constitution, grip strength, and vital capacity of both populations. Methods: The research was done on 422 boys and girls of 1 5-18 years, which consisted of 152 children living in Samigaluh and 270 children living in Galur Kulonprogo. The children were examined with respect to body weight, height, grip strength of right and left hands, and vital capacity. Body Mass was Index then calculated from weight and height. Statistical analysis of three way ANOV A, t-test, Pearson product moment correlation, and linear regression were used.Results: Body constitution, grip strength, and vital capacity of boys differed significantly from girls. Boys were heavier and taller than girls. Grip strength and vital capacity were also greater in boys. Children living in Galur were heavier and taller than those living in Samigaluh, but they were similar in vital capacity. Grip strength was different at age of 15-16 years. There was no difference in Body Mass Index between boys and girls, as well as between Samigaluh children and Galur children.Conclusions: Body constitution, grip strength, and vital capacity of boys and girls were different significantly, also between Samigaluh and Galur children as well. Concerning Samigaluh children were shorter and lighter, it was said that Samigaluh children have better physical performance. Grip strength and vital capacity of 1 5 to 1 8 year old children living in Samigaluh highland and Galur lowland were stronger affected by body constitution than by age.Key words: body constitution; grip strength; vital capacity; 1 5-18 year old boys and girls
Pancreatoblastoma : A case report Indrawati, Indrawati Rahmayani, Susi Hariyati, Soeripto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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A 2.5 year old female child was admitted to Dr. Sardjito Hospital with complaint of enlarging abdomen since 6 months. She was apparently well until 3 months prior to admission when she had anorexia and weight lost. Physical examination revealed a solid intrabdominal mass (15x13 cm) with smooth surface and no tenderness. USG of the abdomen showed a large mass in paraaortal region andpushed the aorta. Operation was done and during exploration the mass was located as high as the stomachlevel and extended into the pancreas. Gross examination showed the tumor was 11x11x8 cm, encapsulated,nodular, white cut surface, some parts were brown and most of them were fragile. The diagnosis ofpancreoblastoma was established by immunohistochemical examination. The presenting features of pancreoblastoma are generally nonspecific and clinically difficult to distinguishfrom other intraabdominal tumors such as neuroblastoma, non-Hodgkin lymphoma, Wilms tumor, hepatoblastoma and desmoblastoma. Some clinical tests might suggest these tumors, i.e. multiorgan involvement for non-Hodgkin lymphoma; renal origin, the propensity for venous invasion and for thepulmonary metastasis for Wilms tumor. a–feto protein that is positive in either hepatoblastoma or pancreoblastoma cannot differ both tumors. Another intrabdominal tumor that should be considered is desmoblastoma that positive vimentin stain. The positive CAM5.2. as well as cytokeratin and the negative vimentin in immunohistochemical examination confirmed the diagnosis of pancreoblastoma.Keywords : pancreoblastoma – child – intraabdominal tumors – immunohistochemical examination
Penile granuloma caused by liquid silicone injection Ishandono Dachlan, Ishandono Dachlan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Various alloplastic injectable implants have been developed for soft tissue augmentation withoutsurgery. Silicone is a polymer from a family of chemically related organo-silicone compounds that mayexist in any state from a fluid to a solid. Injectable liquid silicone has been used for various cosmetictreatments, mainly for soft tissue augmentation, for example lip, cheek, breast, buttock, and penile. Liquidsilicone has been implicated in variety of adverse inflammatory reactions, sometimes resulting in tissuedestruction.We describe an adverse granulomatous reaction after the injection of liquid silicone for penile augmentation.Penile granuloma can occur as an adverse effect of penile enlargement with the injection of high viscositysolution, like silicone (siliconoma), paraffin (paraffinoma), vaseline or mineral oil. Penile granuloma is arare case which needs an accurate management.We found 30-years old man that occurs 5 weeks after non medical injection of liquid silicone. Surgicalmanagement was done with an excision of the granuloma and round incision on the upper penile, exceptthe urethral part. There were no complications following the treatment. Histopathology examination showedthe appearance of silicone-like foreign body granuloma and fibrosis of the preputium. It is concluded that Injection liquid silicone for penile augmentation could cause adverse granulomatous. Surgical management can be done with an excision of the granuloma.Keywords: liquid silicone, penile granuloma, surgical excision.
Body Constitution, grip stregth, and Vital Capacity of Children 15 to 18 Years in Samigaluh Highland and Galur Lowland Kulonprogo Yogyakarta Janatin Hastuti, Janatin Hastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

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Abstract

Background: Growth of children at age of 15 to 18 years old has an important influence on providing body constitution in their adult. Grip strength and vital capacity express functional effort of organs or parts of the body. Therefore, it certainly relates to the shape and size of the body or the body constitution. Different environment in highland and lowland affects the body constitution and functional capacity in growth period.ObJective: The aims of this research were to know the differences of body constitution, grip strength, and vital capacity of 15-18 year old children in Samigaluh highland and Galur lowland in Kulonprogo, Yogyakarta Province, and the relationship between body constitution, grip strength, and vital capacity of both populations. Methods: The research was done on 422 boys and girls of 1 5-18 years, which consisted of 152 children living in Samigaluh and 270 children living in Galur Kulonprogo. The children were examined with respect to body weight, height, grip strength of right and left hands, and vital capacity. Body Mass was Index then calculated from weight and height. Statistical analysis of three way ANOV A, t-test, Pearson product moment correlation, and linear regression were used.Results: Body constitution, grip strength, and vital capacity of boys differed significantly from girls. Boys were heavier and taller than girls. Grip strength and vital capacity were also greater in boys. Children living in Galur were heavier and taller than those living in Samigaluh, but they were similar in vital capacity. Grip strength was different at age of 15-16 years. There was no difference in Body Mass Index between boys and girls, as well as between Samigaluh children and Galur children.Conclusions: Body constitution, grip strength, and vital capacity of boys and girls were different significantly, also between Samigaluh and Galur children as well. Concerning Samigaluh children were shorter and lighter, it was said that Samigaluh children have better physical performance. Grip strength and vital capacity of 1 5 to 1 8 year old children living in Samigaluh highland and Galur lowland were stronger affected by body constitution than by age.Key words: body constitution; grip strength; vital capacity; 1 5-18 year old boys and girls

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