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Articles 14 Documents
Search results for , issue "Vol 41, No 01 (2009)" : 14 Documents clear
The influence of endometriosis peritoneal fluid on the development of mouse embryo in in vitro pre-implantation Hasto Wardoyo, Ita Fauziah, O. Wahyu, Djaswadi Dasuki
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Endometriosis was associated with various immunologic changes detected both in the endometrial eutopic and blood edge sample. Some researchers reported that women with endometriosis have an increased amount of peritoneum fluid. Endometrial environment and peritoneal fluid diffusing to the ovarial tube may affect sperm-ovum interaction, initial phase of embryonic development and reduce endometrial receptability. The correlation between endometriosis and early in vitro embryonic development has been reported in some studies showing controversial results; therefore it is necessary to carry out researches to clarify the influence of endometriosis on the early embryonic development. Objective: to identify the influence of peritoneal fluid of infertile women with endometriosis and normal peritoneum fluid of fertile women on the development of mouse embryo in in-vitro pre-implantation. Methods: Prospective, randomized controlled trial subjects are 2 cells of the Swiss albino strain embryos super-ovulating procedure on mouse, 2 embryonic cells resulted from in-vivo fertilization were taken out and in-vitro culture was done with the exposure of 10% or 2.5% cell-free supernatant peritoneum fluid of infertile women with endometriosis and 10% or 2.5% cell-free supernatant peritoneum fluid of normal fertile women as the control. The blastocyst growth was observed in the 72 hour culture and hatching blastocyst culture in 96 hours. Results: An amount of 231 embryos of 2 cells was obtained. After 72 hours, there were 103 blastocyst cultures in both groups. Blastocyst development rate (BDR) culture with to endometriosis peritoneum fluid exposure was lower compared with the normal peritoneum fluid exposure (32.2% vs 56.9%, RR 0.5, 95% CI: 0.42-0.77). Hatching rate (HRI of blastocyst culture with endometriosis peritoneum fluid was lower than that of normal peritoneum fluid (35.1 % vs 92.4%, RR 0.38, 95%CI: 0.24-0.59). The exposure to 10% endometriosis peritoneum fluid lowered BDR significantly compared with the normal peritoneum fluid, 7.7% vs 43.9% (RR 0.18,95% CI: 0.07-0.42); while the exposure to 2.5 % BDR was significantly different, (64% vs 74%, RR 0.87,95% CI: 0.66-1.13). Exposure to 10% compared to 2.5% normal endometriosis peritoneum fluid showed significantly decreased BDR (p 0.05). There was a significant interaction between type of peritoneum fluid (endometriosis vs normal) and the concentration of peritoneum fluid (10% vs 2.5%1 in decreasing BDR (RR 0.17,95% CI: 0.04-0.65). Conclusion: Exposure to endometriosis peritoneum fluid lowered the blastocyst development rate and hatching rate as compared with that to normal peritoneum fluid on the development of the in- vitro mouse embryo model. Key words: embryonic development - peritoneum fluid - endometriosis
The Change of Retinal Sensitivity in Diabetic Retinopathy after Photocoagulation Angela Nurini Agni, Arif Budiman, Hartono, Angela Nurini A
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Panretinal photocoagulation is used to destroy new blood vessel growth which develops on large areas of retina. Burns that occur would cause scars, that would stop bleeding. Laser has shown benefit in decreasing advance visual loss. Several studies stated that panretinal photocoagulation also decreased the light sensitivity, peripheral visual acuity, and night visual, paracentral scotoma, choroidal neovascularization (rare). submacular fibrosis, and laser injury cycatrical widening, Study on this problem is needed. Objective: To find out the influence of panretinal photocoagulation on retinal sensitivity in diabetic retinopathy patients. Methods: This study used pretest-posttest design. Subjects were diabetic retinopathy patients who visited Ophthalmology Outpatient Clinic Unit Dr. Sardjito Hospital and Dr. Yap Eye Hospital, Yogyakarta from March 2008 to January 2009. All subjects underwent panretinal laser photocoagulation and visual field was examined before treatment and 1 month (4 weeks) after treatment. Data were analyzed descriptively. The difference of retinal sensitivity before and after treatment were analyzed with paired Hest. Result: 19 patients (34 eyes) were included in the study. Average duration of diabetes mellitus in the subjects was 10.44 + 5.26 years. Most (82.4%) subjects had no history of hypertension. The number of laser given to subjects were 826-1850 burns (the average was 1164+218.95). There was improvement in the retinal sensitivity after photocoagulation, although it was statistically insignificant (p > 0.05). There was no significant difference between old and new parameters on visual acuity improvement (p = 0.72 Conclusion: There was no significant change in retinal sensitivity between before and 4 weeks after panretinal photocoagulation in diabetic retinopathy patients. Key words: retinal sensitivity - panretinal photocoagulation - diabetic retinopathy
Standardization of Immunocytochemical method for the diagnosis of Dengue Viral Infection in Aedes aegypti Linn Mosquitoes (Diptera Culicidane) Umniyati, Sitti Rahmah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

methods for virus detection in the mosquito, such as the direct fluorescent-antibody test on head squashes. However, it has the disadvantages of being labor-intensive and requiring fluorescent microscope as well as cryofreezer. Newer methods involving enzyme conjugates such as peroxidase in conjunction with either polyclonal or monoclonal antibodies are greatly improved. With new methods of immunocytochemistry, it is now possible to detect dengue viral antigen in a variety of tissues. Objective: This study was aimed to standardize an immunocytochemical streptavidin-biotin-peroxidasecomplex assay for diagnosis of dengue infection in Aedes aegypti using monoclonal antibody DSSC7. Methods: The infected mosquitoes were held in small cylindrical cages covered with mosquito netting, and incubated at 27:t 1°C ami at relative humidity of 88:t 6 %. The specificity of the immunocytochemical procedure was validated by negative and positive controls showing that the antibody was bound to an appropriate structure. The sensitivity and specificity were also evaluated based on Herrmanns Formula. The presence of dengue antigen on head squash preparation was detected based on ISBPC assay using monoclonal antibody against dengue. The validity and reliability of the measurement were evaluated based on kappa values, according to Landis and Koch. Result: Positive result was detected as discrete brownish granular deposits throughout most visual fields of brain tissue. Dengue viral antigen was immunolocalized to the cytoplasm of brain cells. The immunocytochemical test under light microscope at magnification of 400x was 86.67% sensitive, 96.00% specific, and the kappa value is 0.64. Meanwhile the kappa value between two observers was 0.92, with sensitivity and specificity of 96% and 97% respectively at magnification of 1000x. Conclusion: The monoclonal antibody DSSC7 was sensitive, specific, valid, and reliable as primary antibody to detect dengue viral infection in Ae. aegypti head squash preparation based on immunocytochemical streptavidin-biotin-peroxidase-complex assay under light microscope. Key words: antigen - denguevirus - Aedes aegypti - immunocytochemistry - monoclonal antibody DSSC7
Comparison of daily vs. weekly single-dose ferrous sulphate treatment in female junior high students with iron deficiency anemia Madarina Julia, Dedy Afandi, Sri Mulatsih,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Complianc~ of daily treatment of iron deficiency anemia in children is still low. The compliance will be increased if the iron supplementation is given weekly. Previous study showed that there was no significant difference in the increase in hemoglobin level between daily and weekly treatment in prepubertal children. Objective: To study the difference in the increase in hemoglobin and serum ferritin levels between daily and weekly sirigle-dose treatment of ferrous sulphate in female junior high school students with iron deficiency anemia after menarche. Methods: This was a quasi-experimental study recruiting 179 and 174 anemic female students in the weekly and daily group, respectively. They receive weekly or daily single-dose of ferrous sulphate capsules. Hemoglobin levels were measured before and after the 12 weeks treatment, while serum ferritin levels were measured before and after in a subset of the study subjects. Result: Mean:t SD levels of hemoglobin before and after iron supplementation were 11.18:1: 0.51 and 12.79:t0.63 g/dl (p=0.001) in the weekly group, and 11.17:1:0.61 and 12.68:1:0.57 g/dl (p=0.001) in the daily group. Mean:l: SD levels of ferritin before and after iron supplementation were 6.95:1: 1.85 and 41.5:1:33.93 ng/ml (p=0.001) in the weekly group, and 6.61 :1:2.17 and 40.7:1:22.73 ng/ml (p=0.001) in the daily group. The prevalence of anemia after supplementation is similar in both groups, i.e. 6.7% in the weekly group and 8.0% in the daily group (p = 0.631. There were no significance difference in the occurrence of side effects of diarrhea and nausea in both groups (p>0.05). Conclusion: This study concluded that daily vs. weekly ferrous sulphate supplementation did not result in significantly different level of both hemoglobin and serum ferritin after treatment. The difference in the occurrence of side effects was also not statistically significant. Key words: anemia iron deficiency - hemoglobin level - serum ferritin level - ferrous sulphate
The effect of Pandanus conoideus Lam. oil on pancreatic (i-cells and glibenclamide hypoglycemic effect of diabetic Wistar rats Maliyah Madiyan, Nur Anisah, Winarto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Oxidative stress has been implicated in pathogenesis and progression of many degenerative disorders including naturally occuring and chemically-induced diabetes mellitus. In addition to the increased production of free radicals, antioxidant defense systems are disturbed in diabetes mellitus. As a result, oxidative stress may cause oxidative damage of cellular, including Ii-cells of pancreas. Objective: This study was aimed at investigating the effect of P. eonoideus Lam. on hypoglycemic activity of glibenclamide and histochemical changes in pancreatic islets. Methods: Thirty male Rattus norvegicus rats were used in the study, and randomly selected. The animals were divided into two groups, each group consisted of 15 rats. Each group was devided into 3 subgroups, the first subgroups were subgroup 1; 2, 3 and the second was subgroup 4, 5, 6. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes in the second group. Subgroup 1 and 4 did not receive anything, subgroup 2 and 5 received glibenclamide 0.09 g/kgBW/day and subgroup 3 and 6 received P. eonoideus Lam. 0.3 mLlkgBW/day and glibenclamide 0.09 g/kgBW/day. Rats were given P. eonoideus Lam. and glibenclamide everyday for 14 days. Hypoglycemic activity was evaluated in overnight fasted at day 1, 7 and 14, and the acute hypoglycemic activity was evaluated at time 0, 1, 2, 4, and 6 hour aher treatment at day 14. Blood was collected from orbital sinuses. Blood glucose level was determined using GOD-PAP method. At 15 day rat were killed, the pancreas was removed. The data was compared statistically using one-way analysis of variance (AN OVA) followed by post-hoc test and student unpaired t-test. For the analysis of immunohistochemical data, a non parametric test (Kruskal-Wallis) was used. Results: The combination of P. eonoideus Lam. and glibenclamide produced significant reduction in blood glucose in the serial study at day 1, 7 and 14 and comparable with that of standard drug, glibenclamide also control groups in normal and diabetic rats. It also produced significant reduction of blood glucose in the serial study at time 0, 1, 2, 4, and 6 hour after treatment at day 14. Significant increasing histoscore, number and diameter of Langerhans islets were observed in P. eonoideus Lam. treated rats. Conclusion: P. eonoideus Lam. could increase hypoglycemic effect of glibenclamide and histoscore, number, and diameter of Langerhans islets in diabetic rats. Key words: minyak buah merah - pancreas B-cells - glibenclamide hypoglycemic effect - diabetes
Giant fetus in fetu in a female child with fever: A case report Rochadi, Mei Neni Sitaresmi, Soeripto, Hanggoro Tri Rinonce Pieri Kumaladewi, Siti Iqbalwati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Fetus in fetu is a form of monozygotic diamniotic twin pregnancy where the parasitic twin installs and grows in the body of its partner. It is a rare condition, with a reported incidence of 1 in 500.000 live births. The majority of these cases occur in children younger than 18 months with the chief complaint of an intraabdominal mass, and have a 2:1 male predominance. The size and weight of the fetus vary, from 4 cm to 24.5 cm and from 1.2 g to 1.8 kg. Whether this entity represents a highly differentiated teratoma versus a parasitic twin remains controversial. The objective of this paper was to report a rare condition of giant fetus in fetu in female child with fever, especially to emphasize the importance of proper examination. Although fetus in fetu is very rare, it should remembered as a possibility in an intraabdominal. In some cases further detailed examinations should be done to confirm the diagnosis. A rare case of giant fetus in fetu in a 20th month female patient with fever was reported. Intrabdominal mass was found as the first sign related to fetus in fetu but was neglected by the parents. USG, plain abdominal photo and CT-scan had revealed the diagnosis preoperatively. Proper examination of the fetus in fetu was done including grossly by doing extensive dissection, and detailed histological tissue examination from several sites to make diagnosis completely. Key words: giant fetus in fetu - fetiform teratoma - congenital abnormality - retroperitoneal, fever
The influence of stigma and depression on quality of life of leprosy patients Agnes S. Siswati, Jenny MC Siagian, Carla R Marchira
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Stigma attached to leprosy-affected individuals is one of the strongest stigma of diseases. Depression is often occurred to many lepers. Stigma and depression are two factors which affect the quality of life of the lepers. Objective: To determine whether stigma and depression affect the quality of life of leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Hospital. Methods: It was a quantitative and cross-sectional research. The quantitative analysis was conducted with chi-square and multiple regression methods. Result: There was no significant correlation between stigma and quality of life of leprosy patients. The factors which significantly correlated with quality of life were gender, income, and depression score (p < 0.051. Depression score significantly affected the quality of life (B 1.876; P < 0.05; CI 1.166-36.5661. Conclusion: Depression affected the quality of life more than deformity and demographic factors among leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Yogyakarta. Key words: stigma - depression - lepro
The Change of Retinal Sensitivity in Diabetic Retinopathy after Photocoagulation Angela Nurini Agni, Arif Budiman, Hartono, Angela Nurini A
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

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Abstract

Background: Panretinal photocoagulation is used to destroy new blood vessel growth which develops on large areas of retina. Burns that occur would cause scars, that would stop bleeding. Laser has shown benefit in decreasing advance visual loss. Several studies stated that panretinal photocoagulation also decreased the light sensitivity, peripheral visual acuity, and night visual, paracentral scotoma, choroidal neovascularization (rare). submacular fibrosis, and laser injury cycatrical widening, Study on this problem is needed. Objective: To find out the influence of panretinal photocoagulation on retinal sensitivity in diabetic retinopathy patients. Methods: This study used pretest-posttest design. Subjects were diabetic retinopathy patients who visited Ophthalmology Outpatient Clinic Unit Dr. Sardjito Hospital and Dr. Yap Eye Hospital, Yogyakarta from March 2008 to January 2009. All subjects underwent panretinal laser photocoagulation and visual field was examined before treatment and 1 month (4 weeks) after treatment. Data were analyzed descriptively. The difference of retinal sensitivity before and after treatment were analyzed with paired Hest. Result: 19 patients (34 eyes) were included in the study. Average duration of diabetes mellitus in the subjects was 10.44 + 5.26 years. Most (82.4%) subjects had no history of hypertension. The number of laser given to subjects were 826-1850 burns (the average was 1164+218.95). There was improvement in the retinal sensitivity after photocoagulation, although it was statistically insignificant (p > 0.05). There was no significant difference between old and new parameters on visual acuity improvement (p = 0.72 Conclusion: There was no significant change in retinal sensitivity between before and 4 weeks after panretinal photocoagulation in diabetic retinopathy patients. Key words: retinal sensitivity - panretinal photocoagulation - diabetic retinopathy
Standardization of Immunocytochemical method for the diagnosis of Dengue Viral Infection in Aedes aegypti Linn Mosquitoes (Diptera Culicidane) Umniyati, Sitti Rahmah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

methods for virus detection in the mosquito, such as the direct fluorescent-antibody test on head squashes. However, it has the disadvantages of being labor-intensive and requiring fluorescent microscope as well as cryofreezer. Newer methods involving enzyme conjugates such as peroxidase in conjunction with either polyclonal or monoclonal antibodies are greatly improved. With new methods of immunocytochemistry, it is now possible to detect dengue viral antigen in a variety of tissues. Objective: This study was aimed to standardize an immunocytochemical streptavidin-biotin-peroxidasecomplex assay for diagnosis of dengue infection in Aedes aegypti using monoclonal antibody DSSC7. Methods: The infected mosquitoes were held in small cylindrical cages covered with mosquito netting, and incubated at 27:t 1°C ami at relative humidity of 88:t 6 %. The specificity of the immunocytochemical procedure was validated by negative and positive controls showing that the antibody was bound to an appropriate structure. The sensitivity and specificity were also evaluated based on Herrmann's Formula. The presence of dengue antigen on head squash preparation was detected based on ISBPC assay using monoclonal antibody against dengue. The validity and reliability of the measurement were evaluated based on kappa values, according to Landis and Koch. Result: Positive result was detected as discrete brownish granular deposits throughout most visual fields of brain tissue. Dengue viral antigen was immunolocalized to the cytoplasm of brain cells. The immunocytochemical test under light microscope at magnification of 400x was 86.67% sensitive, 96.00% specific, and the kappa value is 0.64. Meanwhile the kappa value between two observers was 0.92, with sensitivity and specificity of 96% and 97% respectively at magnification of 1000x. Conclusion: The monoclonal antibody DSSC7 was sensitive, specific, valid, and reliable as primary antibody to detect dengue viral infection in Ae. aegypti head squash preparation based on immunocytochemical streptavidin-biotin-peroxidase-complex assay under light microscope. Key words: antigen - denguevirus - Aedes aegypti - immunocytochemistry - monoclonal antibody DSSC7
Comparison of daily vs. weekly single-dose ferrous sulphate treatment in female junior high students with iron deficiency anemia Madarina Julia, Dedy Afandi, Sri Mulatsih,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Complianc~ of daily treatment of iron deficiency anemia in children is still low. The compliance will be increased if the iron supplementation is given weekly. Previous study showed that there was no significant difference in the increase in hemoglobin level between daily and weekly treatment in prepubertal children. Objective: To study the difference in the increase in hemoglobin and serum ferritin levels between daily and weekly sirigle-dose treatment of ferrous sulphate in female junior high school students with iron deficiency anemia after menarche. Methods: This was a quasi-experimental study recruiting 179 and 174 anemic female students in the weekly and daily group, respectively. They receive weekly or daily single-dose of ferrous sulphate capsules. Hemoglobin levels were measured before and after the 12 weeks treatment, while serum ferritin levels were measured before and after in a subset of the study subjects. Result: Mean:t SD levels of hemoglobin before and after iron supplementation were 11.18:1: 0.51 and 12.79:t0.63 g/dl (p=0.001) in the weekly group, and 11.17:1:0.61 and 12.68:1:0.57 g/dl (p=0.001) in the daily group. Mean:l: SD levels of ferritin before and after iron supplementation were 6.95:1: 1.85 and 41.5:1:33.93 ng/ml (p=0.001) in the weekly group, and 6.61 :1:2.17 and 40.7:1:22.73 ng/ml (p=0.001) in the daily group. The prevalence of anemia after supplementation is similar in both groups, i.e. 6.7% in the weekly group and 8.0% in the daily group (p = 0.631. There were no significance difference in the occurrence of side effects of diarrhea and nausea in both groups (p>0.05). Conclusion: This study concluded that daily vs. weekly ferrous sulphate supplementation did not result in significantly different level of both hemoglobin and serum ferritin after treatment. The difference in the occurrence of side effects was also not statistically significant. Key words: anemia iron deficiency - hemoglobin level - serum ferritin level - ferrous sulphate

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