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Articles 14 Documents
Search results for , issue "Vol 46, No 03 (2014)" : 14 Documents clear
Correlation between leptin level with lipid profile and free fatty acid in liver cirrhosis patients Siti Nurdjanah, Siti Maryani, Neneng Ratnasari,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/jms.v46i03.7308

Abstract

Malnutrition is a common condition in liver cirrhotic patients. Leptin regulates body weightphysiologically by suppressing appetite and increasing energy expenditure. Leptin is higher in femalethan male. Studies have shown correlation between leptin with metabolic factors like body massindex (BMI) and lipid profile in cirrhotic patients. This study was conducted to investigate thecorrelation between serum leptin levels with lipid profile and free fatty acid in male patients with livercirrhosis. This was a cross sectional study that conducted at Gastroentero-Hepatology Clinic andInternal Ward at Dr. Sardjito General Hospital, Yogyakarta. The inclusion criteria were patients withliver cirrhosis > 18 years old, male, with Child-Pugh classification B and C, and provided informedconsent. The exclusion criteria were liver cirrhotic patients with comorbidity chronic kidney disease,chronic heart failure, diabetic, cancer, infection/septic, pregnancy, breast feeding, and steroid use.Data collecting was performed by anamnesis, physical examination, abdominal ultrasonographyexamination, and blood chemistry test. Pearson test was used to evaluate the correlation betweenthe serum leptin level with the lipid profile and free fatty acid. The results showed that no significantnegative correlation was observed between the serum leptin level with the total cholesterol (r= -0.052; p=0.766), high-density lipoprotein/HDL (r= -0.078; p=0.658) and triglyceride (r= -0.170; p=0.328) in male patients with liver cirrhosis. Furthermore, no significant positive correlationwas observed between the serum leptin levels with the low-density lipoprotein/LDL (r= -0.013; p=0.942) and free fatty acid/FFA (r= 0.007; p=0.968). In conclusion, there was no correlationbetween serum leptin levels with lipid profile and FFA in male patients with liver cirrhosis.
Zinc deficiency and school-age children’s memories Indria Laksmi Gamayanti, Setianingsih, Djaswadi Dasuki,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.861 KB) | DOI: 10.19106/JMedScie004603201403

Abstract

Zinc deficiency is associated with cognitive and motor delay in children. Moreover, it is associatedwith deficits in activity and attention in nutritional deficiency children. This study was conductedto evaluate the correlation between zinc deficiency with memory of children. A cross sectionalstudy design was employed from June 1st to 30th, 2013 among school-age children in KlatenDistrict, Central Java who met inclusion and exclusion criteria. Sixty five eligible children wereassessed their health, socio-economic and nutritional status. Hemoglobin levels were measuredby the standart cyanoblue method. Plasma zinc levels were analyzed with flame atomic absorptionspectrophotometry (AAS). Short-term memory (STM) was measured using the instrument WechslerIntellegence Scale for Children (WISC0 subtest Digit Span Memory Test and long term memory(LTM) was assessed using the recall of narrative. Independent t-test was used to compare theSTM or LTM between groups of each independent factors. Linear regression analysis was usedto determine the independent factors associated with the STM or LTM. The STM scores of thechildren with zinc deficiency (6.1 ± 1.3) was significantly higher than those with normal zinclevel (10.7 ± 3.1) [p=0.0004; 95%CI= -6.98 – (-2.14)]. However, the LTM for both group ofthe children were not significantly different (p=0.658; 95%CI= -3.16 – 2.01). A significantcorrelations between zinc levels, hemoglobin level and socio-economic status with the STM scoreswere observed (p<0.05), whereas nutritional status was not (p>0.05). In contrast, no significantcorrelations between zinc levels, hemoglobin levels, socio-economic status, nutritional status andthe LTM scores was observed in the school age children (p>0.05). In conclusion, zinc deficiency isassociated with STM loss in the school age children, however it is not associated with LTM loss.Moreover, hemoglobin level and socio-economic status are found to be independent factors forSTM loss, however they are not independent factors for LTM loss.
The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model Arief Budiyanto, Widodo Wirohadidjojo, Retno Danarti, Suswardana,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.34 KB) | DOI: 10.19106/JMedScie.004603201401

Abstract

Povidone-iodine (PI) 10% solution is an effective antiseptic. However, it appears to be toxic tothe cells involved in wound healing. The aim of this study is to evaluate the toxicity of PI oncultured human fibroblast using fibroblast populated collagen lattice (FPCL) model. The culturedhuman fibroblast was divided into 6 groups i.e. 5 groups were exposed by PI 1, 0.1, 0.01, 0.001and 0.0001%, and 1 group was exposed by phosphate-buffered-saline (PBS). Twenty-four hourslater, the media was washed using PBS. The size of the FPCL media on each group wasobserved over time by serial photographs, which then were measured by Image-J computerprogram. Exposure of 0.1, 0.01, 0.001 and 0.0001 PI caused an obvious reduction of fibroblast’scontraction capability on FPCL media, which described temporary fibroblast injury, that showinga concentration-dependent recovery phenomenon after 48th hour. Furthermore, 1% PI exposureleads to a permanent fibroblast injury. In conclusion, PI exposure in concentration more than0.1% has a permanent toxic effect on fibroblast that clearly observed using a simple FPCLmodel.
Binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase Glial Fibrillary Acidic Protein (GFAP) expression of astrocytes in the CA1 rat hippocampus during operant learning conditioning Nugraha, Zainuri Sabta; Aswin, Soedjono; Harijadi, Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.092 KB) | DOI: 10.19106/JMedScie004603201401

Abstract

Astrocytes of hippocampus contribute in the learning performance. Entrainment of gamma wavescan improve learning performance by improving the neurons to astrocytes communication. Theaim of this study was to evaluate the effect of binaural entrainment of 2000-2040 Hz and 2000-2090 Hz on Glial Fibrillary Acidic Protein (GFAP) expression in astrocytes of the CA1 region ofrat hippocampus during operant learning conditions. Twenty male Wistar rats aged 4-6 weekswith body weight 100-150 g were devided into 4 groups. Group I was given binaural soundsentrainment at 2000-2040 Hz without learning test. Group II was given entrainment as performedin Group I followed by a learning test. Group III was given binaural sounds entrainment at 2000-2090 followed by a learning test. Group IV was not given entrainment nor learning test. Theentrainment was performed for 30 minutes everyday for 12 days and the learning test wasperformed for 10 minutes everyday for 12 days. The GFAP expression was examinedimminohistochemically. Astrocytes processes and astrocytes histoscore were also calculated.The results showed that the number of the GFAP-positive astrocytes in Group I (70.96 ± 4.86),II (69.76±3.07) and III (63.10±5.85) were significantly higher than Control (47.33±1.33)(p<0.05). The number of the processes astrocytes in Group I (47.64 ± 3.87), II (60.66±2.07)and III (54.17±6.38) was significantly higher than Control (30.87±2.69) (p<0.05). Moreover,the number of the processes astrocytes in Group II was significantly higher than Group I (p=0.016).The astrocytes histoscore index in the Group II (115.58±14.13) and III (78.32±22.23) weresignificantly higher than Group I (28.79 ± 9.61) and Control (16.05±1.64) (p<0.05). Inconclusion, the binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase GFAP expressionof astrocytes in Cornu Ammonus 1 (CA1) region of rat hippocampus during operant learningconditioning.
Secondary acute myeloblastic leukemia after adjuvant chemotherapy and radiotherapy in breast cancer: A case report Kartika Widayati Taroeno-Hariadi, Mardiah Suci Hardianti, Johan Kurnianda,
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/jms.v46i03.7311

Abstract

A rising number of long-term survivors and potential long-term outcome related to the treatment isone of the most important issue due to the advances of management in cancer. The development ofsecondary malignancy has been reported in a number of cohorts. We present a case of breast cancerpatient developing acute myeloid leukemia (AML)- M5 within a relatively short interval of two and ahalf years from her primary treatment with adjuvant chemotherapy with 4 cycles of doxorubicin andcyclophosphamide, followed by 4 cycles of three weekly paclitaxel (AC-T) and radiotherapy. Whatcould be attributed to the occurrence of secondary leukemia in this patient will be discussed.
Current in vitro assay to determine bacterial biofilm formation of clinical isolates Nuryastuti, Titik
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.978 KB) | DOI: 10.19106/JMedScie004603201406

Abstract

Biofilm mode of growth is bacterial lifestyle widespread in microbial world and represents a concernin health care. Recently, serious problem concerning biofilm-related infection is increasing significantly.However complexity of biofilm makes it difficult to conduct proper analysis. Although biofilm representsa major challenge for microbiologist, methods aimed to determine biofilm formation and developmentare not standardized yet. The aim of the present review was to provide an overview of the advantagesand disadvantages of the difference in vitro methods for determining biofilm forming ability ofclinical isolates.
Correlation between leptin level with lipid profile and free fatty acid in liver cirrhosis patients Maryani, Siti; Ratnasari, Neneng; Nurdjanah, Siti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.354 KB) | DOI: 10.19106/JMedScie004603201404

Abstract

Malnutrition is a common condition in liver cirrhotic patients. Leptin regulates body weightphysiologically by suppressing appetite and increasing energy expenditure. Leptin is higher in femalethan male. Studies have shown correlation between leptin with metabolic factors like body massindex (BMI) and lipid profile in cirrhotic patients. This study was conducted to investigate thecorrelation between serum leptin levels with lipid profile and free fatty acid in male patients with livercirrhosis. This was a cross sectional study that conducted at Gastroentero-Hepatology Clinic andInternal Ward at Dr. Sardjito General Hospital, Yogyakarta. The inclusion criteria were patients withliver cirrhosis > 18 years old, male, with Child-Pugh classification B and C, and provided informedconsent. The exclusion criteria were liver cirrhotic patients with comorbidity chronic kidney disease,chronic heart failure, diabetic, cancer, infection/septic, pregnancy, breast feeding, and steroid use.Data collecting was performed by anamnesis, physical examination, abdominal ultrasonographyexamination, and blood chemistry test. Pearson test was used to evaluate the correlation betweenthe serum leptin level with the lipid profile and free fatty acid. The results showed that no significantnegative correlation was observed between the serum leptin level with the total cholesterol (r= -0.052; p=0.766), high-density lipoprotein/HDL (r= -0.078; p=0.658) and triglyceride (r= -0.170; p=0.328) in male patients with liver cirrhosis. Furthermore, no significant positive correlationwas observed between the serum leptin levels with the low-density lipoprotein/LDL (r= -0.013; p=0.942) and free fatty acid/FFA (r= 0.007; p=0.968). In conclusion, there was no correlationbetween serum leptin levels with lipid profile and FFA in male patients with liver cirrhosis.
Secondary acute myeloblastic leukemia after adjuvant chemotherapy and radiotherapy in breast cancer: A case report Hardianti, Mardiah Suci; Kurnianda, Johan; Taroeno-Hariadi, Kartika Widayati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.544 KB) | DOI: 10.19106/JMedScie004603201405

Abstract

A rising number of long-term survivors and potential long-term outcome related to the treatment isone of the most important issue due to the advances of management in cancer. The development ofsecondary malignancy has been reported in a number of cohorts. We present a case of breast cancerpatient developing acute myeloid leukemia (AML)- M5 within a relatively short interval of two and ahalf years from her primary treatment with adjuvant chemotherapy with 4 cycles of doxorubicin andcyclophosphamide, followed by 4 cycles of three weekly paclitaxel (AC-T) and radiotherapy. Whatcould be attributed to the occurrence of secondary leukemia in this patient will be discussed.
Zinc deficiency and school-age children’s memories Setianingsih, .; Dasuki, Djaswadi; Gamayanti, Indria Laksmi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.861 KB) | DOI: 10.19106/JMedScie004603201403

Abstract

Zinc deficiency is associated with cognitive and motor delay in children. Moreover, it is associatedwith deficits in activity and attention in nutritional deficiency children. This study was conductedto evaluate the correlation between zinc deficiency with memory of children. A cross sectionalstudy design was employed from June 1st to 30th, 2013 among school-age children in KlatenDistrict, Central Java who met inclusion and exclusion criteria. Sixty five eligible children wereassessed their health, socio-economic and nutritional status. Hemoglobin levels were measuredby the standart cyanoblue method. Plasma zinc levels were analyzed with flame atomic absorptionspectrophotometry (AAS). Short-term memory (STM) was measured using the instrument WechslerIntellegence Scale for Children (WISC0 subtest Digit Span Memory Test and long term memory(LTM) was assessed using the recall of narrative. Independent t-test was used to compare theSTM or LTM between groups of each independent factors. Linear regression analysis was usedto determine the independent factors associated with the STM or LTM. The STM scores of thechildren with zinc deficiency (6.1 ± 1.3) was significantly higher than those with normal zinclevel (10.7 ± 3.1) [p=0.0004; 95%CI= -6.98 – (-2.14)]. However, the LTM for both group ofthe children were not significantly different (p=0.658; 95%CI= -3.16 – 2.01). A significantcorrelations between zinc levels, hemoglobin level and socio-economic status with the STM scoreswere observed (p<0.05), whereas nutritional status was not (p>0.05). In contrast, no significantcorrelations between zinc levels, hemoglobin levels, socio-economic status, nutritional status andthe LTM scores was observed in the school age children (p>0.05). In conclusion, zinc deficiency isassociated with STM loss in the school age children, however it is not associated with LTM loss.Moreover, hemoglobin level and socio-economic status are found to be independent factors forSTM loss, however they are not independent factors for LTM loss.
Correlation between leptin level with lipid profile and free fatty acid in liver cirrhosis patients Maryani, Siti; Ratnasari, Neneng; Nurdjanah, Siti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.354 KB) | DOI: 10.19106/JMedScie004603201404

Abstract

Malnutrition is a common condition in liver cirrhotic patients. Leptin regulates body weightphysiologically by suppressing appetite and increasing energy expenditure. Leptin is higher in femalethan male. Studies have shown correlation between leptin with metabolic factors like body massindex (BMI) and lipid profile in cirrhotic patients. This study was conducted to investigate thecorrelation between serum leptin levels with lipid profile and free fatty acid in male patients with livercirrhosis. This was a cross sectional study that conducted at Gastroentero-Hepatology Clinic andInternal Ward at Dr. Sardjito General Hospital, Yogyakarta. The inclusion criteria were patients withliver cirrhosis > 18 years old, male, with Child-Pugh classification B and C, and provided informedconsent. The exclusion criteria were liver cirrhotic patients with comorbidity chronic kidney disease,chronic heart failure, diabetic, cancer, infection/septic, pregnancy, breast feeding, and steroid use.Data collecting was performed by anamnesis, physical examination, abdominal ultrasonographyexamination, and blood chemistry test. Pearson test was used to evaluate the correlation betweenthe serum leptin level with the lipid profile and free fatty acid. The results showed that no significantnegative correlation was observed between the serum leptin level with the total cholesterol (r= -0.052; p=0.766), high-density lipoprotein/HDL (r= -0.078; p=0.658) and triglyceride (r= -0.170; p=0.328) in male patients with liver cirrhosis. Furthermore, no significant positive correlationwas observed between the serum leptin levels with the low-density lipoprotein/LDL (r= -0.013; p=0.942) and free fatty acid/FFA (r= 0.007; p=0.968). In conclusion, there was no correlationbetween serum leptin levels with lipid profile and FFA in male patients with liver cirrhosis.

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