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el-Hayah
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Articles 120 Documents
IDENTIFICATION OF VESICULAR ARBUSCULAR MYCORRHIZA (VAM) FROM SOIL AND ITS POTENCY IN REDUCING DISEASE DEVELOPMENT (PHYTOPHTHORA SP.) ON 5 CITRUS ROOTSTOCK Dwiastuti, Mutia Erti; Widyaningsih, Sri; Wicaksono, Rudi Cahyo; Agustina, Dina; Triasih, Unun
El-Hayah Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8426

Abstract

Phytophthora spp. is one of the fungal pathogens that kills plants on several kinds of the citrus rootstock. In other countries, it is reported that disease pathogens were reduced in roots containing Vesicular Arbuscular Mycorrhiza (VAM interaction). However, in Indonesia, there is less information about the effect of VAM on the roots of citrus plants against root disease caused by Phytophthora sp. This study aimed to identify VAM in citrus roots and study the potential of VAM in controlling root rot of Phytophthora sp. on five types of the citrus rootstock. The research was carried out at the Phytopathology Laboratory, Indonesian Citrus, and Subtropical Fruits Research Institute (ICSFRI). Phytophthora spp. and VAM samples originated from several citrus centers endemic to Phytophthora were collected. VAM was isolated from the rhizosphere area of citrus plants, while Phytophthora sp. was isolated from infected plant roots. The fungus isolates were isolated, purified, then identified through references. The test of the potential of VAM in increasing resistance of root diseases caused by Phytophthora sp. was performed at the screen house in ICSFRI. The results of the study showed that VAM was identified in 39 gardens in 6 districts from samples collected in 49 yards in 10 regions of citrus centers. The dominant VAM genus is Glomus sp. with the highest density of spores was originated from Ponorogo area. The results of the identification of Phytophthora morphologically showed a diversity of Phytophthora, namely P. parasitica, P. palmivora, and P. citrophthora. The test of the potential of VAM in increasing plant resistance to Phytophthora results showed that Kanci, JC, RL, and Volkameriana varieties inoculated with Phytophthora sp. and Glomus sp. have higher plant height than healthy plants.
ANTIBACTERIAL ACTIVITY ETHYL ACETATE EXTRACTS OF EARTHWORMS (LUMBRICUS RUBELLUS, EISENIA FOETIDA, NEREIS SP) TOWARD STAPHYLOCOCCUS AUREUS, ENTEROCOCCUS FAECALIS, SALMONELLA THYPOSA INVITRO Kartikaningsih, Hartati; Maharani, Sarastria; Sartika, Fitarina
El-Hayah Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8248

Abstract

Earthworms had a mechanism of antibacterial. The research aimed to observe Lumbricus rubellus, Eisenia foetida, Nereis sp. antibacterial activity against Salmonella thyposa, Staphylococcus aureus, Enterococcus faecalis in vitro compared to ampicillin antibiotics. All the worms extracted using ethyl acetate extraction and tested their MIC. The compound of amino acids of the worms was analyzed by HPLC and nanodrop.  Lumbricus rubellus was the best anti-bacteria activity followed by Eisenia foetida and Nereis sp., but these activities less than ampicillin antibiotic. Observations with SEM showed these worms extract caused cell leakage in all of these bacteria. Protein content with Nanodrop testing revealed the highest protein content was Lumbricus rubellus (21.75 ppm) followed by Eisenia foetida (21.32 ppm) and Nereis sp. (20.98 ppm), as well as for amino acids levels, there were Lumbricus rubellus (24.66%), Eisenia foetida (22.78%), Nereis sp. (18.37%). From the 15 amino acids detected, all of the worms had the same sequence of fourth the highest amino acids (Glutamate, Aspartate, Leucine, Arginine) and fourth the lowest amino acid levels (Methionine, Hystidin, Tyrosin, Glisan). It had not been tested amino acid sequences of antibacterial compounds of these worms (Lumbricin 1: Phe-Ser-Lys-Tyr-Glu-Arg in Lumbricus rubellus worms, Fetidin 1: Ala-Met-Val-Ser-Ser and Fetidin 2: Ala-Met- Val-Gly-Thr in the Eisenia foetida worm, Hemerythrin: His-Glu-Asp in Nereis sp).
THE ROLE OF POTASSIUM AND CALSIUM IN IMPROVING THE QUALITY AND SHELF LIFE OF TOMATO (LYCOPERSICUM ESCULENTUM VAR. SERVO) Wicaksana, Puguh Catur; Wijaya, Ketut Anom; Soeparjono, Sigit
El-Hayah Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8430

Abstract

Tomato has the characteristics natural to damage. Damage fruits are caused by destitute of fruit quality and sensory attributes. The loss of tomato production on harvest in developing countries reached 50% due to the long duration of tomato distribution. Servo varieties of tomatoes are in demand by farmers because of their genetic advantages, high productivity, and abundant fruit weights. The shortage of servo varieties of tomatoes is the short shelf life of fruit, which is seven days after harvest. Short shelf life is influenced by fruit thickness and fruit hardness. In improving the quality and shelf life of tomatoes, potassium (K) nutrition plays a role in photosynthetic translocation, strengthens the cell wall, and involved in the lignification process of sclerenchyma tissue. Tomatoes, as climacteric fruits have a high respiration rate influenced by ethylene. Ethylene production can be inhibited by calcium chloride (CaCl2) application by strengthening the cell wall mechanism, cross-link with pectin, and increase the cell wall regeneration can change the texture of the fruit become harder, and it reduces sensitivity to physiological damage. Based on the treatment of this research, the quality and shelf life of servo varieties of tomato is increased.
THE EFFECT OF BIOACTIVATOR VARIATION AND DOSES OF COW DUNG ON QUALITY OF COFFEE EXOCARP WASTE Krismawati, Amik; Sugiono, Sugiono
El-Hayah Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8243

Abstract

Coffee exocarp waste produced from the harvest can be used as raw material for compost. The composting can be added with other ingredients to add organic material. Cow manure is the one ingredient that can be added to enrich organic materials. In the composting process, the time required will be longer, but the time can be accelerated by adding a bio activator. The finished compost can be applied at the plant to meet crop nutrient elements. The purpose of this research was to determine the effect of bio activator variation and doses of cow manure on the quality of compost from coffee exocarp waste. This research was conducted at compost house of Assessment Institute for Agricultural Technology (AIAT) and was held on February - April 2017. This research used factorial Randomized Block Design (RBD) with two factors; there is a variation of bio activator and doses of cow manure with three replication. Total number of treatment this research were 12 treatment, there are D1S1 = EM4 + 2 kg cow manure ; D2S1 = Moebillin + 2 kg cow manure; Petrofast + 2 kg cow manure; Decoprima + 2 kg cow manure; D1S2 = EM4 + 4 kg cow manure; D2S2 = Moebillin + 4 kg cow manure; D3S2 = Petrofast + 4 kg cow manure; D4S2 = Decoprima + 4 kg cow manure; D1S3: EM4 + 6 kg cow manure; D2S3 = Moebillin + 6 kg cow manure; D3S3 = Petrofast + 6 kg cow manure; D4S3 = Decoprima + 6 kg cow manure. The data obtained will be processed using Analysis of Variance (ANOVA). If there is a real effect, it will be continued using the BNT test (Least Significant Differences/LSD) with a 5% level. The Results showed the application of bio activator Decoprima (D4) and a dose of 6 kg cow manure (S3) could increase and shows the highest value of pH compost 6,01 (D4) and 6,06 (S3). The dose of 2 kg cow manure (S1) showed the highest compost shrinkage value (34.64%) compared to the 4 kg dose cow manure (S2) (32,22%) and 6 kg (S3) (25,68%). On the other hand, the application of bio activator variation and doses of cow manure did not significantly affect the quality of compost, including N-total (2,15% -2,60%), C-organic (21,40% -24,91%) and C/N ratio (8,81-11,15). The physical properties of the aroma compost show the smell of soil, and the color of the compost is dark brown. 
THE SUCROSE CONTENTS OF FOUR HONEY TYPES FROM APIS MELLIFERA BEEKEEPERS IN JAVA Adalina, Yelin; Kuntadi, Kuntadi
El-Hayah Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8244

Abstract

Honey is a sweet thick liquid made by honeybees as their main products. Due to the increasing demand for honey, the risk of counterfeiting is on the rise, mainly when the production is limited. In term of quality standards, the sucrose content is one of the benchmarks in determining the authenticity of honey. This study aims at determining the sucrose contents of the honey from six regencies in Java. Ten honey samples that consisted of two samples of kapok honey, three samples of mango honey, three samples of rubber honey, and two samples of coffee honey were obtained from Apis mellifera beekeepers. The results showed that the averages of sucrose contents in mango honey, rubber honey, coffee honey, and kapok honey were respectively 4.94%, 3.47%, 10.49%, and 1.02%. The sucrose contents of the ten honey samples averagely met the benchmark. Still, two honey samples exceeded the criterion outlined in SNI-2013 (maximum of 5%), i.e., mango honey from Sragen Regency and coffee honey from the Pasuruan Regency. The high sucrose content of coffee honey was allegedly induced by honey counterfeiting through the addition of sugar solutions.
VIABILITAS DAN PRODUKTIVITAS SELULOSA DARI INOKULUM KERING Acetobacter xylinum DENGAN SUBSTRAT PEMBAWA BERUPA SERBUK KELAPA PARUT DAN SERBUK AMPAS KELAPA PARUT Andriani, Amalia Fitri
el–Hayah Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i1.1681

Abstract

Inokulum nata yang berisi kultur Acetobacter xylinum, pada umumnya tersedia dalam bentuk agar slant atau bentuk kultur cair dalam medium air kelapa. Bentuk inokulum tersebut membutuhkan perlakuan khusus dan mahal. Seiring dengan penigkatan kebutuhan inokulum nata de coco, maka bentuk inokulum dikembangkan agar lebih praktis, mudah perlakuannya, penyimpanan dan aman dalam transportasi. Penelitian ini bertujuan untuk mengetahui kemungkinan penyediaan inokulum kering nata de coco dengan substrat pembawa berupa serbuk kelapa parut dan serbuk ampas kelapa parut. Inokulum kering dibuat dengan menginokulasikan kultur cair A. xylinum ke dalam serbuk kelapa parut kering dan serbuk ampas kelapa parut kering kemudian dilakukan pengeringan dengan inkubator dengan suhu 30o C dan 40o C. Inokulum dalam bentuk serbuk kering dengan substrat pembawa berupa serbuk kelapa parut dan serbuk ampas kelapa parut dapat dibuat dengan pengeringan suhu 30o C selama 30 jam dengan perbandingan substrat pembawa : inokulum cair sebanyak 1:2. Inokulum kering dengan substrat pembawa berupa serbuk kelapa parut memiliki kadar air 3,25 %, vabilitas 1,0 x 107 sel/g dan produktivitas selulosa 5,55 g/L. Inokulum kering dengan substrat pembawa berupa serbuk ampas kelapa memiliki kadar air 2,98 %, viabilitas 4,2 x 105 sel/g dan produktivitas selulosanya sebasar 4,92 g/L. Produktivitas selulosa inokulum kering tersebut setara dengan 80% produktivitas selulosa hasil inokulum cair dari isolat A. xylinum asal. nata de coco, inokulum kering, Acetobacter xylinum, selulosa bakteri, substrat pembawa
STUDI DAYA HAMBAT IN VITRO ANTI MPS ECTO CIK (MAYOR PHYSIOLOGICAL PROTEIN SUBSTRAT) TERHADAP VIABILITAS SPERMATOZOA KAMBING DAN DOMBA Muchtaromah, Bayyinatul
el–Hayah Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i1.1682

Abstract

Untuk menuju suatu penemuan tentang vaksin kontrasepsi bagi pria, diperlukan penelitian dasar mengenai hal tersebut. Sebagai langkah awal dilakukan uji hambatan Anti MPS ecto CIK terhadap viabilitas spermatozoa kambing dan domba, sedangkan dari penelitian pendahuluan diketahui bahwa anti MPS dari ecto-CIK ini mampu bereaksi silang dengan spermatozoa domba, dan sapi. Berdasarkan hal tersebut peneliti tertarik untuk mengetahui seberapa besar peranan anti MPS ecto CIK dalam menghambat  viabilitas spermatozoa kambing dan domba secara in vitro. Tujuan dari penelitian ini adalah mengetahui seberapa besar peranan anti MPS ecto CIK dengan pemberian perlakuan konsentrasi dan lama inkubasi serta interaksi kedua perlakuan dalam menghambat viabilitas spermatozoa kambing dan domba. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan pola faktorial yang terdiri atas 2 faktor utama, faktor pertama yaitu: dosis pengenceran, 0µl, 5µl, 10µl, dan 15µl, dan faktor kedua yaitu: lama inkubasi, yaitu 5 menit, 30 menit, 60 menit, dan 120 menit, masing-masing 6 kali ulangan. Data viabilitas spermatozoa kambing dan domba  dianalisis dengan Uji one way ANOVA, jika hasil dari analisis tersebut terdapat pengaruh yang nyata maka akan dilanjutkan dengan Uji Jarak Berganda Duncan dengan taraf signifikansi 5% (0,05).Pemberian perlakuan anti MPS dari ecto CIK membran spermatozoa kambing dengan konsentrasi dan 0 µl, 5 µl, 10 µl, 15 µl dan lama inkubasi 5 menit, 30 menit, 60 menit dan 120 menit berpengaruh signifikan dalam menghambat viabilitas spermatozoa kambing dan domba. Pada perlakuan anti MPS ecto CIK dengan konsentrasi 15 µl dan lama inkubasi 120 menit merupakan perlakuan yang paling optimal dalam menghambat viabilitas spermatozoa kambing (45,50 ± 11,16%; 44,87 ± 9,40%) dan domba (55,54 ± 18,87%; 40,58 ± 13,20%).  Interaksi pemberian perlakuan konsentrasi dan lama inkubasi anti MPS ecto CIK pada konsentrasi 15 µl dan lama inkubasi 120 menit anti MPS ecto CIK paling berpengaruh dalam menghambat viabilitas spermatozoa domba (26,83 ± 8,70%), sedangkan pada kambing tidak berpengaruh.
PEMBUATAN PATI TINGGI AMILOSA SECARA ENZIMATIS DARI PATI UBI KAYU (Manihot esculenta) DAN APLIKASINYA UNTUK PEMBUATAN MALTOSA DEA, Dr.Ir. Yunianta,
el–Hayah Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i1.1683

Abstract

Sebagian besar pati alami seperti pati jagung, gandum, tapioka, kentang dan sagu mengandung prosentase yang tinggi dari rantai percabangan amilopektin. Tingginya komponen amilopektin dalam tapioka tersebut merupakan salah satu kendala dalam pemanfaatan tapioka secara meluas dalam berbagai industri. Agar dihasilkan pati dengan amilosa tinggi maka salah satu alternatifnya adalah memodifikasi pati secara enzimatis dengan debranching enzymes yaitu enzim yang mampu menghidrolisis ikatan α-1,6 pada pati. Aplikasi pati tinggi amilosa dalam penelitian ini adalah untuk pembuatan maltosa.Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi tapioka dan lama inkubasi terhadap kadar amilosa yang dihasilkan oleh enzim pullulanase dan mengetahui pengaruh penambahan enzim -amilase pada tapioka dengan amilosa tertinggi  terhadap maltosa yang dihasilkan. Rancangan percobaan yang digunakan pada tahap I adalah Rancangan Acak Kelompok yang disusun secara faktorial dengan dua faktor, faktor I adalah konsentrasi tapioka (5, 10, 15, dan 20%) dan faktor dua adalah lama inkubasi (12 dan 24 jam) pada larutan 2% enzim pullulanase. Masing-masing perlakuan diulang sebanyak 3 kali. Data yang diperoleh dianalisa dengan ANOVA yang dilanjutkan dengan uji DMRT. Sedangkan pada tahap II menggunakan metode eksperimen secara deskriptif.Hasil penelitian tahap I menunjukkan bahwa dari perlakuan kombinasi konsentrasi substrat tapioka dan lama inkubasi, kadar amilosa tertinggi diperoleh pada konsentrasi tapioka 15% dengan lama inkubasi 12 jam yaitu sebesar 41,12%, dengan kadar pati 77,46%, viskositas 358,33 cP dan kadar air 4,01%. Penelitian tahap II menunjukkan hasil hidrolisis tapioka tinggi amilosa oleh β-amilase adalah maltosa 30,84%, maltotriosa 2,90%, glukosa 0,27% dan oligosakarida 5,42%. Sedangkan untuk tapioka tanpa proses debranching menghasilkan maltosa 26,47%, maltotriosa 1,86%, glukosa 0,28% dan oligosakarida 13,83%.
THE GENETIC POTENCY OF PLANTAIN AGUNG SEMERU VARIETY FROM LUMAJANG REGENCY EAST JAVA INDONESIA Prahardini, P.E.R.
el–Hayah Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i1.1684

Abstract

Lumajang regency is one of the banana production centre in East Java having high- diversity of banana germ plasm.  There are 33 cultivars of  banana germ plasm in the regency,   consist of eaten ripe and plantain. One of uniqe plantain used as the symbol of Lumajang regency is plantain Agung Semeru variety, the local superior variety of this regency.  This variety can grow well at 450 – 650 m above sea level.  The uniqueness of banana Agung Semeru variety can be seen by the number of sucker per cluster (only   1 – 2  suckers per cluster), the size of the finger (33 – 36 cm long and  19 cm around) and the number of hand per bunch (only 1 – 2 hand per bunch).  Other characteristics of the variety are the thickness of fruit skin, the long period of fruit storage  (3 – 4 weeks after harvesting) and  the sweetness of fruit flesh. Even though the skin changes from yellow to black,  the flesh still  can be consumed, because it  doesn’t become soft. This variety also resistant to the  Sigatoka disease compared to other plantain cultivars.
EFEKTIFITAS PENGGUNAAN CHITOSAN SEBAGAI AGEN ANTIMIKROBA PADA DAGING AYAM BROILER L., Rahayu,
el–Hayah Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Jurusan Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i1.1685

Abstract

Penelitian penggunaan chitosan dengan konsentrasi yang berbeda sebagai larutan perendaman daging ayam broiler selama 5 menit serta penyimpanan 8 jam telah dilakukan untuk mengetahui pengaruhnya terhadap total mikroba dan nilai TVBN. Hasil penelitian menunjukkan bahwa perendaman daging ayam dalam larutan chitosan dengan konsentrasi yang berbeda berpengaruh nyata (P<0,05) terhadap total mikroba, dan tidak berpengaruh terhadap nilai TVBN. Setelah penyimpanan 8 jam daya hambat total mikroba yang paling tinggi yaitu konsentrasi 2% sebesar 4,4 x 106 CFU/g.

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