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Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 234 Documents
Construction of Begomovirus AV1 Gene Candidate into pBI121 and Its Introduction into Tobacco by using Agrobacterium tumefaciens Vector Santoso, Tri J.; Herman, Muhammad; Hidayat, Sri H.; Aswidinnoor, Hajrial; ., Sudarsono
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
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Abstract

Infection of Begomovirus has caused leaf curl disease in tomato. This infection has significantly impact on yield losses of tomato production. Recently, in Indonesia there was no effectively way to control this disease.  The use of resistant tomato variety is one of strategies to control this virus. Genetic engineering technology gives an opportunity to develop the transgenic tomato resistant to Begomovirus through pathogen derived resistance (PDR) approach. The objectives of this study were to construct the  Begomovirus AV1 candidate gene in the pBI121 and to introduce the construct into tobacco plant genome through Agrobacterium tumefaciens vector. A series activites in gene construct have been conducted include PCR amplification of AV1 gene using a pair of specific primer, cloning the gene into pGEM-T easy, transformation of the clone into Escherichia coli DH5α competent cell, construct the gene into pBI121, and transform the construct into A. tumefaciens. Leaf segments of in vitro tobacco plant were transformed by co-cultivation with A. tumefaciens containing ToLCV-AV1 construct. In the research activitiy, Indonesian  Begomovirus  AV1 gene  was  successfully amplified and inserted in expression vector plasmid pBI121. Tobacco transformants carrying kanamycin-resistant gene (nptII gene) were regenerated and established in the glasshouse. Those transformant plants are expected containing the AV1 gene.
AvrBs3/PthA Virulence Factor of Bacterial Leaf Blight Race III, Race IV, Race VIII, and IXO93-068 Utami, Dwinita W.; Kadir, Triny S.; Yuriyah, Siti
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
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Bacterial leaf blight (BLB) is an important disease of rice and present throughout many of the rice-growing regions in the world, also in Indonesia. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent and a member of the Protebacteria and like many other this phyllum have a type III secretion system for protein virulence effector (PVE) released on their patho-genicity system. Commonly, PVE in Xanthomonas sp., is coded by AvrBs3/PthA family gene. This research was coducted to identify the virulence factor of AvrBs3/PthA on dominant Indonesian BLB isolates (Race III, Race IV, Ras VIII, and IXO93-068). This objective was obtained by sequence analysis through designed markers for members of the virulence factor AvrBs3/PthA gene family (PthXo4, avrXa7#38, PthXoS and avrXa7sacB50). Results gave infor-mation that RaceIII is a dependent elicitor race due to no PVE transcript formed and intraceluler protein target with RLL type on NLS (nuclear localization signal). RaceIV and RaceVIII are the virulent race which PVE active formed with intraceluler protein target and have the RLL and RLLP type for the NLS signal. While isolate IXO93-068 is a virulen isolate that active formed a PVE but the extraceluler protein target is due to no type of NLS. Based on cluster analysis, Race VIII has a genetic distance closely to PthXoS and avrXa7sacB50.
Efficacy of RB gene in transgenic potato Katahdin SP904 and SP951 to West Java isolates of Phytophthora infestans Ambarwati, A. Dinar; Sumaraw, S. M.; Purwito, Agus; Herman, M.; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
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Potato late blight, caused by Phytophthora infestans is one of the most devastating plant disease. Potato yield losses due to this disease ranged from 47-100%. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum. RB gene has been integrated into cultivated potato Katahdin using Agrobacterium-mediated transfor-mation, and showed durable and broad spectrum resistance either in laboratory assay or in confined field trial. Evaluation of transgenic Katahdin SP904 and SP951 was conducted to verify whether the RB gene with broad spectrum to all known races of P. infestans in the United States and in Toluca, Mexico was also effective against P. infestans isolates in Indonesia. Efficacy of RB gene was evaluated for foliar and tuber resistance to West Java isolates. Transgenic Katahdin were more resistant in foliar than non transgenic plants, at 14 days after inoculation. Diseases intensity of transgenic Katahdin SP904 and SP951 were 19.8-43.8%, whereas non transgenic Katahdin, Granola, and Atlantic were 46.9-100%. In contrast to the foliar resistance phenotype, RB-containing tubers in transgenic Katahdin did not exhibit increased resistance to Lembang, Pangalengan and Galunggung isolates. Tubers of transgenic Katahdin SP904, SP951, and non transgenic Katahdin showed lesion volume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene in transgenic Katahdin showed efficacy against late blight P. infestans in foliar, but did not showed efficacy in tuber. Transgenic Katahdin RB thus providing a potential source of resistance for breeding programs.
Delivering of Over-Expression Construct OsWRKY76 Candidate Gene in Rice cv. Nipponbare through Agrobacterium tumefaciens Apriana, Aniversari; Sisharmini, Atmitri; Enggarini, Wening; Sudarsono, Sudarsono; Khumaida, Nurul; Trijatmiko, Kurniawan R.
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
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Abstract

Plant genetic improvement can be done through classical breeding or genetic engineering. WRKY is a transcription factor involved in regulating plant defense responses. OsWRKY76 gene is located in a narrow segment of chromosome 9 which is identified previously to be related to wide spectrum resistance in rice. A sequence of OsWRKY76 (+1.200 bp) has available in the gene bank and it makes possible to isolate, clone, and construct the gene into over-expression vector. The aim of this research was to assemble an over-expression construct of OsWRKY76 candidate gene and introduce it into rice through Agrobacterium-mediated transformation. A construct of pCAMBIA-1301::35S::OsWRKY76 has been successfully assembled and transformed into embryogenic calli of rice cv. Nipponbare using A. tumefaciens strain Agl-1 and EHA 105. A number of 126 independent lines has been produced, in which Agl-1 showed 3.8 times more efficient than EHA 105. PCR analysis of randomly selected 25 independent lines showed that all of them positively contained hptII gene, a selectable marker used in the over-expression construct of the OsWRKY76 candidate gene. Based on the result, it could be concluded that the over-expression construct of OsWRKY76 candidate gene have been successfully introduced into the tissue of Nipponbare.
Phylogenetic and Maturity Analyses of Sixty Soybean Genotypes Used for DNA Marker Development of Early Maturity Quantitative Trait Loci in Soybean Tasma, I Made; Satyawan, Dani; Warsun, Ahmad; Yunus, Muhamad; Santosa, Budi
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
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The Indonesian soybean productivity is still very low with the national average of 1.3 t/ha. One means to improve national soybean productivity is by manipulating harvest index by cultivating very early maturing soybean cultivars. Development of early maturing soybean cultivars can be expedited by using marker-aided selection. The objective of this study was to select parental lines having contrasted maturity traits and selected parents must be genetically distance. The parents then were used to develop F2 populations for detecting early maturity QTL in soybean. Maturity tests of 60 soybean genotypes were conducted at two locations, Cikeumeuh (Bogor) and Pacet (Cianjur) using a randomized block design with three replications. Genomic DNA of the 60 genotypes were analyzed using 18 SSR markers and genetic relationship was constructed using the Unweighted Pair-Group Method Arithmatic through Numerical Taxonomy and Multivariate System program version 2.1-pc. Results showed that the 60 genotypes demonstrated normal distribution in both locations for days to R1 (32-48d), days to R3 (35-55d), days to R7 (75-92d), and days to R8 (78-99d). Four early maturing genotypes and three late genotypes were obtained. Total SSR alleles observed were 237 with average allele per locus of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89). Genetic similarity among genotypes ranges from 74.8-95%. At similarity level 77% divided the genotypes into six clusters (the four selected early maturing genotypes located in clusters III and IV, while the three late genotypes located in cluster II). Based on maturity data, pubescent color, and phygenetic analysis seven parents were selected (four early maturing genotypes B1430, B2973, B3611, B4433 and three late genotypes B1635, B1658, and B3570). Twelve F2 populations were developed with the aid of SSR markers Satt300 dan Satt516. Two of the populations will be used to develop DNA markers for earliness in soybean.
Increasing the Growth and Quality of Red Chili with Growth Hormone from Endophytic Bacteria Yuniawati, Rafika; Fatimah, Siti; Indrayanti, Reni; Manzila, Ifa; Priyatno, Tri Puji; Susilowati, Dwi Ningsih
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p75-82

Abstract

Red chili is a very important horticultural commodity in Indonesia having low productivity and quality. Cultivation method needs to be improved including the use of exogenous growth hormones. The purpose of this study was to determine (1) the optimum concentration of IAA and GA growing hormones from isolate B6.2 in stimulating plant growth and improving the quality of large red chili fruit; (2) molecular identity of the B6.2 bacterial isolate. The growth hormone content of B6.2 isolates using HPLC obtained 0.49 ppm IAA and 64.53 ppm GA. The growth hormone potential test on the growth and quality of chili was carried out with a concentration of 1, 3, 5 ml/l, while water and synthetic hormones was used as negative and positive control, respectively. The experimental design used was a Complete Random Design with the foliar spray application to the plant canopy three times during the growth period. The results showed the best concentration in increasing plant height, fruit weight, shooth wet, and dry weight compared to controls at the age of 76 days after planting (dap) was a concentration of 5 ml/l, with the values of 71.7±0.9 cm , 94.7±0.3 g, 11.5±0.43 g, and 1.4±0.09 g, respectively. The molecular identification showed that B6.2 isolate was classified as Bacillus vallismortis with 100% homology. The growth hormone from isolate B6.2 has the potential to increase growth and production of red chili plants.
Back Matter JA Vol 15 No 1 AgroBiogen, Jurnal
Jurnal AgroBiogen Vol 15, No 1 (2019): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n1.2019.p%p

Abstract

Rice Anther Culture to Develop Double Haploid Population and Blast Resistant Lines Ambarwati, Dinar; Soemantri, Ida H.; Utami, Dwinita W.; Apriana, Aniversari; Moeljopawiro, Sugiono
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p71-77

Abstract

Penyakit blas pada padi yang disebabkanoleh cendawan Pyricularia grisea, merupakan salah satukendala dalam produksi beras. Sumber gen ketahanan terhadappenyakit blas dijumpai pada spesies padi liar Oryzarufipogon. Populasi silang ganda (BC2F3) turunan IR64 danO. rufipogon mempunyai QTL untuk sifat ketahanan terhadappenyakit blas. Untuk mempercepat perolehan tanamanhomosigot dari populasi tersebut, dilakukan kultur anterpada dua media induksi kalus: I1 (N6 + NAA 2 mg/l + kinetin0,5 mg/l + sukrosa 60 g/l + putresin 0,16 g/l) dan I2 (N6 +2,4-D 2 mg/l + sukrosa 50 g/l) dan dua media regenerasi: R1(MS + NAA 0,5 mg/l + kinetin 2 mg/l + sukrosa 40 g/l +putresin 0,16 g/l) dan R2 (MS + NAA 1 mg/l + kinetin 2 mg/l+ sukrosa 30 g/l). Kultur anter dilakukan pada sembilan genotipe,di mana tiga genotipe (149-16, 343, 337-13) memberikanrespon terbaik dalam produksi planlet hijau setelahdikulturkan pada media regenerasi R1. Dari 208 planlet hasilregenerasi diperoleh 42 planlet haploid ganda dari genotipe149-16, 11 planlet haploid ganda dari genotipe 343, dan 44planlet haploid ganda dari genotipe 337-13. Skrining ketahananblas di rumah kaca pada populasi haploid gandamenghasilkan 46 tanaman tahan terhadap ras 001, 33 tanamantahan terhadap ras 033, dan 79 tanaman tahan terhadapras 173. Sebanyak 28 tanaman bersifat tahan, baik terhadapras 001, 033, maupun 173 seperti halnya O. rufipogon.Galur-galur homosigot ini akan diuji di lapang untuk ketahanannyaterhadap penyakit blas dan karakter agronominya.
Molecular Analysis and Phenotypic Performances of BC3F2 Upland Rice Lines Containing Alt and Pup1 Loci Mizan, Muhammad Rauful; Wirnas, Desta; Tasliah, nFN; Hidayatun, Nurul; Prasetiyono, Joko
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p83-92

Abstract

The challenges in upland rice cultivation are phosphorous (P) deficiency and aluminum (Al) toxicity, each controlled by Pup1 and Alt loci, respectively. Pyramiding the two genes into Indonesian rice varieties were previously done through Marker-Assisted Backcrossing method to obtain BC3F2 populations. The aims of this study were to analyze the BC3F2 upland rice lines containing the Alt and Pup1 loci molecularly (foreground and background analyses) and to test their phenotypic performances. Genetic materials tested included Dupa variety (donor of Alt) and three improved Indonesian genotypes (Dodokan-Pup1, Situ Bagendit-Pup1, and Batur-Pup1) as recurrent parents, Kasalath (donor of Pup1), and 300 BC3F2 lines from Dodokan-Pup1+Alt, Situ Bagendit-Pup1+Alt, and Batur-Pup1+Alt, respectively. The rice genotypes were selected individually using modified Yoshida nutrient solution, followed by foreground and background analyses. 150 out of 300 seedlings were selected and maintained until harvest in the greenhouse. Foreground analysis using markers (RM1361, RM12031, and Kas46-2) and tiller number performances resulted in 18 plants from BC3F2 Dodokan-Pup1+Alt, 30 plants from BC3F2 Situ Bagendit-Pup1+Alt, and 25 plants from BC3F2 Batur-Pup1+Alt still carrying Alt and Pup1 loci. Background analysis using molecular markers showed that the best individual lines of BC3F2 were number 56 for BC3F2 Dodokan-Pup1+Alt, number 32 or 70 for BC3F2 Situ Bagendit-Pup1+Alt, and number 20 for BC3F2 Batur-Pup1+Alt. The selected lines having both both Alt and Pup1 loci in homozygote condition with highest number of tiller per plant which are useful genetic materials for developing upland rice variety tolerance to low P and Al toxicity.
Fron Matter JA Vol 15 No 2 AgroBiogen, Jurnal
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p%p

Abstract

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