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Research Journal of Life Science
Published by Universitas Brawijaya
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Articles 121 Documents
The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei Suleman, Suleman; Andayani, Sri; Yuniarti, Ating
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.393 KB) | DOI: 10.21776/ub.rjls.2018.005.03.3

Abstract

This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.
Antioxidant Effect of Centella asiatica Ethanolic Extract to Superoxide Dismutase (SOD) Level on Cyprinus carpio Liver Jatayu, Diklawati; Nursyam, Happy; Hertika, Asus Maizar Suryanto
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.896 KB) | DOI: 10.21776/ub.rjls.2018.005.03.4

Abstract

Centella asiatica is a herbal medicine has been used for many studies in some animals as antioxidant properties. This study was aimed to enhance the level of SOD in Cyprinus carpio after oral administration of C. asiatica extract. In the present study, phytochemical screening of bioactive constituents, antioxidant assay, and their effect on SOD in C. carpio liver were investigated. C. asiatica extract was obtained by maseration for 24 h using ethanol as a solvent. C. asiatica extract with 50, 100, 150 and 300 mg kg-1 body weight were administered every 3 days for two weeks. On the last day of experiment, fishes were killed and liver were removed from the body. The results showed that C. asiatica extract had some bioactive compounds such as flavonoid, alkaloid, terpenoid, tanin, saponin and DPPH scavenging activity with an IC50 value of 125 μg mL-1. C. asiatica extract also enhanced the level of SOD in fish liver. The SOD level was significantly difference compared to the control group with P<005. These results indicate that the ethanolic extract of C. asiatica had a potential antioxidant properties by scavenging free radical and enhancing the SOD level as antioxidant deffense inside the body.
Identification of Koi Herpesvirus on Koi Fish (Cyprinus carpio) with Immunocytochemistry Test Streptavidin Biotin Wahyuningtyas, Rika; Yanuhar, Uun
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.292 KB) | DOI: 10.21776/ub.rjls.2018.005.03.2

Abstract

Koi herpesvirus (KHV) has been identified in common carp (Cyprinus carpio) and varieties of C. carpio such as koi carp. Handling KHV outbreak on koi fish are always late and on case KHV infection, hence the purpose of this study is to find an alternative method in identifying a particular fish diseases KHV which more simple, practical and accurate.  20 samples are positively infected by KHV were derived from Blitar, with the characteristic of them are gill slime, erosion or ulcers on the skin, movement were not controlled, and skin blister. The result of our study showed that 100% positive reaction to the ICC in smear blood, which is marked golden chocolate color. The result of immunostaining streptavidin biotin obtained that koi fish have been infected by severe KHV showed golden chocolate color on the blood smear compared to positive control. While fish have been infected by undemanding KHV infection or still in the beginning stage, the blood smear showed chocolate bluish. It can be concluded that Immunocytochemistry Test Streptavidin Biotin can be used for early identifying of Koi Herpes Virus (KHV) due to simpler producer, more practical, and accurate result.
Activity of Compounds on Seaweed Eucheuma cottonii Extract as Antioxidant Candidate to Prevent Effects of Free Radical in Water Pollution Wulandari, Dewi; Kilawati, Yuni; Fadjar, Mohamad
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.638 KB) | DOI: 10.21776/ub.rjls.2018.005.03.5

Abstract

Water pollution can make many problems such as, the incidence of disease and poisoning. Pollution in water can produce free radicals and it is can trigger of disease for aquatic organisms. There is some efforts that can be done to provide this problem, such as chemical compound that can reduce the reaction of free radicals. Antioxidants are one of the chemical compounds that can reduce the activity of free radicals. Eucheuma cottonii is the one of a seaweed that has many in antioxidant compounds, such as phenol compounds, but it is also rich in iodine fiber and other important minerals. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of seaweed extraction. Identification of Eucheuma cottonii extract is using FTIR test. The last stage is an antioxidant activity test that includes DPPH test (1,1-diphenyl-2-picrilhidrazil) and Inhibition Concentration 50 (IC 50). The results obtained in this study were based on FTIR test of antioxidant compound in Eucheuma cottonii seaweed extract. The one of compounds that have antioxidant activity include galaktosa-4-sulfat. Based on the results of antioxidant activity test using DPPH obtained that seaweed extract Eucheuma cottonii active as an antioxidant to ward off free radicals in the waters. The concentration of Eucheuma cottonii seaweed extract for preventive 50% concentration of DPPH is 39,926 ppm.
Association Between Time Spent for Internet Gaming, Grade Point Average and Internet Gaming Disorder Risk Among Medical Students Kurnianingsih, Nia; Ratnawati, Retty; Yudhantara, Dearisa Surya; Prawiro, Rizqi Bagus Setyo; Permatasari, Maryam; Rachma, Heidyana; Ariadi, Adilla Surya
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.701 KB) | DOI: 10.21776/ub.rjls.2018.005.03.1

Abstract

The negative and positive consequences of internet games were less explored among medical students’ population. The aim of this current study was to investigated the association between time spent for playing internet games and Grade Point Average (GPA) as well as Internet Gaming Disorder (IGD) risk among medical students. A total of 438 students in academic year 2016-2017 completed the self-report questionnaire, that covered socio-demographic information, GPA, time spent for internet gaming/day, and IGD screening. The statistical analysis used c2 test. The average age of the respondents was 20.2 ±1.57 year. The respondents comprised of 37.13% male and 62.87% female students. The percentage of male and female players was 97.5% and 93.8% respectively. Male students were more at risk of IGD (29.56%) compared to their female counterparts (10.04%). Although time spent for internet gaming/day was associated with IGD risk (p<0.001), it was not associated with GPA (p=0.501). There was no significant GPA difference between students who played internet games <3 hours/day and >3 hours/day and those who never play at all. Further study is required to explore the factors related to GPA among medical students.
Histopathological in Gills, Hepatopancreas and Gut of White Shrimp (Litopenaeus Vannamei) Infected White Feces Disease (WFD) Anjaini, Jefri; Fadjar, Mohamad; Andayani, Sri; Agustin, Ivana; Bayu, Ilham
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (620.851 KB) | DOI: 10.21776/ub.rjls.2018.005.03.6

Abstract

White Feces Disease which is the presence of white feces floating on the surface of the water. Physical disruption of White Feces Disease attacked by exocytes becoming soft. the cause of white defect disease (WFD) is a decrease in environmental quality such as salinity, DO and ammonia. white feces disease is associated with gregarine protozoa in the Apicomplexa phylum. Gregarine parasitic infection from the type of nematopsis that was interacted with Vibrio and Vibrio type bacteria found in white shrimp (Litopenaeus vannamei) which was attacked by white impurities was V. harveyi. This study aims to analyze the presence of gregarine protozoa in the gills, intestines and hepatopancreas of white shrimp infected with White Feces Disease. The method used in this research is descriptive method. Histopathological results showed that there were protozoa (Gregarine) in the intestine, hepatopancreas and gills of white shrimp infected with WFS in 3 different locations and found Aggregated Transformed Microvilli (ATM) that resembled gregarine.
Komposisi Serangga Kanopi di Kebun Apel di Poncokusumo, Malang dan Bumiaji, Batu Leksono, Amin Setyo; Yanuwiadi, Bagyo; Hasyim, M Asmuni; Apituley, Frank Leonardo
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.12 KB) | DOI: 10.21776/ub.rjls.2015.002.01.1

Abstract

This research abjective is to analyze the difference of abundance, diversity and composition of canopy insect in Poncokusumo and Bumiaji on blossoming and fruiting seasons. Insect compostions were observed by regular sampling using blue and yellow  water pan trap. Water pan traps were suspended on apple stands at 2 m height. Sampling efforts was done by block system on 5 trees with 4 times collection, resulting 20 replications. Difference of mean was tested using general linear model analysis of variance. The canopy insect compositions between location were compared using Bray-Curtis similarity index. Overall, canopy insect diversity in two study sites was low. The abundance and diversity of canopy insect in Poncokusumo was higher than that in Bumiaji. The abundance and diversity of canopy insect in yellow traps was higher than that in blue traps. The effect of flowering phenology was significant on the overall abundance but not for the diversity. The effect of study locations on the abundance interacted with season. This meant there was a seasonal variation afecting location.
Adiposopathy In Vitro Study The Effect of Toxoplasma Gondii Profilin Induction To The Expression of IL-6 and TNF-a as A Predictor Candidate of Adipocyte Dysfunction on Subcutan Adipocyte Culture Sudjari, Sudjari; Susanto, Hendra; Indra, Rasjad
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (660.033 KB) | DOI: 10.21776/ub.rjls.2015.002.01.2

Abstract

Metabolic syndrome is a group of several risk factors consist of hypertension, dyslipidemia, glucose intolerance disorder, obesity, and has a relationship with CVD pathomechanism. WHO was report in 2003 more than 300 million adult in the world suffered from obesity. The role of adipose tissue in infectious diseases and adipocyte quality changes after exposure to pathogen has not been revealed. On the other hand, the prevalence of infectious diseases in developing country is still high. Previous studies proved a relationship between infection of Toxoplasma gondii and tissue dysfunction. Profilin is a membrane component of Toxoplasma gondii that able to stimulate expression of proinflammation cytokines through TLR-11 upregulation, and predicted able to trigger the adipocyte dysfunction. The aim of this study are to knows the effect of profilin exposure in vitro on subcutaneous adipocyte to the expression of IL-6 and TNF-α. The measurement of IL-6 and TNF-a levels was done by ELISA technique. The result showed profilin exposure doses 5, 15 and 25 ng / ml increasing IL-6. Profilin exposure cause adipocyte dysfunction through the increasing proinflammatory adipocytokine levels as pathomechanism of metabolic syndrome associated with adipose tissue.
Genetic Polymorphism at Acaca Locus and Its Relationship With Productive Performances in Ettawa Crossbred Goat Maylinda, Sucik; Susilorini, Tri Eko; Surjowardojo, Puguh
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.852 KB) | DOI: 10.21776/ub.rjls.2015.002.01.3

Abstract

Research with aim to estimate genetic polymorphism at ACACA (Acetyl-coenzyme A carboxylase ) locus in Ettawa Crossbred goat wan its relationship with production traits was done at goat population in Batu, Lawang and Ampel Gading. 46 female goats were taken it’s blood sample to isolate the DNA and continue with PCR (Polymerase Chain Reaction) and RFLP (Restricted Fragment Length Polymorphism). PCR was used to amplify ACACA gene fragment in intron 3’ about 200 bp with primer F : 5’ – AGT GTA GAA GGG ACA GCC CAG C – 3’ and R : 5’ – GTG GAA TGA CAC ATG GAG AGG G – 3’; RFLP was used to test mutation of that fragment in particular place (point) using restriction enzyme RSA1. Variables were alelles and genotypes composition in population, milk and fat content, and birth weight of kid. Result showed that (a) genetic polymorphism at locus ACACA in three location was high that is 44,22 %, with allele frequency of G (p) = 33 % and allele T (q) = 67 %; (b) no relationship between the high polymorphism with productive performance of goat in fat and protein content, and birth weight of kid. It was concluded that in goat population there was a high polymorphism at ACACA gene, and that polymorphism was not related to production.
Respon Proliferasi, Diferensiasi dan Ekspresi C/EBPa Akibat Paparan Quercetin pada Kultur Preadiposit Tikus (Rattus Norvegicus) Strain Wistar Secara In Vitro Ratnawati, Retty; Satuman, Satuman; Hernowati, Tinny Endang
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.856 KB) | DOI: 10.21776/ub.rjls.2015.002.01.4

Abstract

Introduction. Obesity is a metabolic syndrome as risk factor to the cardiovascular disease. Obesity occurs caused by adipocyte massive that is started by proliferation and differentiation of preadipocytes that involves transcription factors such as C/EBPα ,PPARγ and SREBP-1. Is known the TNF α and [Ca2+]i Increase when adiponectin decrease at the end of adipocyte differentiation. Therefore it is a deemed treat the obesity through the inhibition of adipocyte proliferation and differentiation using natural resources. Quercetin in due of the flavonoid found in apple,vegetables etc. The aim of this study is to prove the affectivity of quercetin to inhibit proliferation and differentiation of preadipocyte through inhibited of C/EBPα in rat preadipocytes culture method.Methods. Their study was a laboratory experimental. Quercetin was exposed to the preadipocytes human culture, after it was induced by differentiation stimulator the doses quercetin was grouped in: control, 50 µM (Q50), 125 µM (Q125) and 625 µM (Q625) groups. The amount of proliferation and differentiation was descriptive analyzed. The expression of C/EBPα, were identified by Immunocytochemistry and western blotting.Result. Quercetin 100 inhibited the rate of proliferation cell, while quersetin 625 inhibited differentiation in preadipocytes culture. There was a dose dependent to quersetin towards the decrease of PPARγ expression and C/EBPα expression in this preadipocytes culture.Conclusion. It is conclusion that quersetin inhibits proliferation and differentiation preadipocytes culture through the decrease of the expresion of C/EBP α.

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