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Research Journal of Life Science
Published by Universitas Brawijaya
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Articles 9 Documents
Search results for , issue " Vol 1, No 1 (2014)" : 9 Documents clear
Mutual Partnership Model between Plant Breeder and Gapoktan-Ponpes (A Traditional Farmer Group) in Pre-releasing New Hybrid and Composite Varieties of Sweet Corn Sugiharto, Arifin Noor; Suryanto, Agus
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.421 KB) | DOI: 10.21776/ub.rjls.2014.001.01.9

Abstract

We have been genotyping the Filius (F6) sweet corn lines that currently ready to be evaluated by multi-location and heterosis test.Normally, in the step of breeding program, releasing varieties followed by seed production and distribution would be done after such tests that  costly needed and time consuming. Traditional institution of farmer group called in Indonesia as “Gapoktan” and the Religious teaching stage called as “Ponpes” have been  known as non-formal institution which have high potency and unique networking access to surrounding public citizen and other informal institutions.  As so did, they can be as a producer and virtual guarantor or good marketing agent of many agricultural products as well. Therefore, a mutual partnership model established between breeder and them is very important and to be a prospective idea to short cut breeding process and hybrid seed production. Scientific accountability and efficiency of whole breeding – seed production would be kept into main consideration. The concept was “Gapoktan” and “Ponpes” firstly would be trained to adopt technology for crossing to set F1 Hybrid seed, then the results would be evaluated and compared with those done by breeder to determine whether they eligible or not. If they did so, partnership would be mutully continued and they would be encouraged to distribute F1 seeds to other “gapoktan” or public citizens. Three nominated Gapoktan and Ponpes from separated regencies, i.e., Pasuruan, Malang and Batu were determined as the model.The results showed that F1 seed production variable as indicator achievement of them were not significantly different from those of breeder in any place. Even though in between the places they did differ in several vegetative and productive factors each other. The differences were probably caused by ecological factors, not by their capability in agriculture practicing. Based on standard of common farming of corn or sweet corn in East Java, benefit gain increment by farmer caused by such partnership in F1seed production reached about 150% to 247%. So that, partnership model for producing multi-location test as well as producing hybrid seeds between Breeder-Gapoktan-Ponpes was economically prospective and scientifically eligible to do so. On the benefit consideration gained by farmers, Pasuruan was the place where the most attractive to be done partnership model followed by Malang and Batu.
Analisis Restorasi Ekosistem Wilayah Pesisir Terpadu Berbasis Co-Management: Studi Kasus Di Kecamatan Ujung Pangkah Dan Kecamatan Bungah, Kabupaten Gresik Rudianto, Rudianto
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (810.321 KB) | DOI: 10.21776/ub.rjls.2014.001.01.8

Abstract

Penelitian ini dilakukan dengan fokus melakukan restorasi ekosistem wilayah pesisir dengan pendekatan keterpaduan di Kabupaten Gesik dengan mengambil contoh di dua kecamatan yaitu Kecamatan Ujung pangkah dan Kecamatan Bungah. Restorasi merupakan upaya untuk memperbaiki kembali kondisi wilayah pesisir yang sudah rusak lingkungannya akibat dari kegiatan manusia yang makin tidak rasional dan karena proses alam yang perubahannya sangat dtrastis akibat pengaruh global warming. Restorasi berupaya untuk melakukan perbaikan untuk mengembalikannya ke kondisi semula, walaupun untuk mencapai pemulihan kepada kondisi semula masih memerlukan penelitian lebih lanjut. Hal ini mengingat kondisi yang mendukung seperti kondisi hidrologi sudah mengalami perubahan.Metode yang digunakan untuk mencapai tujuan penelitian ini digunakan pendekatan kombinasi yaitu penelitian diskriptif, penelitian partisipatif dan penelitian eksploratif. Penelitian diskriptif terkait dengan observasi dilapang untuk menilai kondisi kedua kabupaten baik secara biogeofisik, sosial ekonomi dan sosial budaya. Untuk penelitian partisipatif berkaitan dengan menjaring aspirasi dan harapan masyarakat melalui wawancara. Sedangkan penelitian eksploratif digunakan model Partial Least Square (PLS) dan Analitycal Hierarchy Process (AHP). Disamping itu untuk merumuskan model restorasi ekosistem terkait pengelolaan digunakan model co-management.Hasil penelitian menunjukkan bahwa hubungan antara masyarakat dengan upaya restorasi ekosistem ternyata sangat kuat berperan, termasuk upaya restorasi perlu dilakukan secara terpadu. Hal ini ditunjukkan oleh hasil PLS. Sedangkan ekosistem pesisir mana yang harus didahulukan untuk ditangani AHP menyatakan bahwa prioritas utama adalah mangrove, kemudian terumbu karang, diikuti dengan kawasan estuari dan terakhir padang lamun. Berdasarkan itu maka model co-management mensyaratkan perlu adanya kolaborasi diantara masyarakat, pemerintah dan swasta. Kolaborasi tersebut dipayungi oleh kerangka strategi yang memuat visi, misi, tujuan dan prioritas strategi.
Pengaruh Waktu Panen Terhadap Kandungan Glukomannan Pada Umbi Porang (amorphophallus muelleri blume) Periode Tumbuh Ketiga Chairiyah, Nurul; Harijati, Nunung; Mastuti, Retno
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.039 KB) | DOI: 10.21776/ub.rjls.2014.001.01.6

Abstract

Waktu panen diduga berpengaruh terhadap kandungan glukomannan pada umbi porang. Penelitian ini bertujuan untuk mengetahui pengaruh waktu panen terhadap dinamika kandungan glukomannan pada umbi porang. Glukomannan diekstraksi dari umbi porang yang diperoleh pada saat dua minggu sebelum tanaman rebah, pada saat tanaman rebah dan dua minggu setelah tanaman rebah. Glukomannan diperoleh dengan menggunakan metode ekstraksi yang telah dimodifikasi. Parameter yang diamati meliputi kandungan glukomannan dan berat kering umbi. Data kandungan glukomannan pada umbi dianalisis dengan One way ANOVA dan dilanjutkan dengan Tukey α 0,05. Sedangkan hubungan antara kandungan glukomannan terhadap berat umbi dianalisis dengan menggunakan Uji Korelasi Bivariate. Dari hasil analisis, Kadar glukomannan cenderung lebih tinggi pada umbi porang yang dipanen saat tanaman rebah dibandingkan dengan glukomannan pada umbi yang diperoleh ketika sebelum dan setelah tanaman rebah yaitu sebesar 29,10 ± 4,57 g/100 g. Peningkatan kandungan glukomannan dapat meningkatkan berat umbi porang.
The Effect of Lactobacillus Acidophilus Microcapsule Which Encapsulated by Kappa Caragenan Toward in Vivo Functional Test Setijawati, Dwi; Firdaus, Muhammad; Kartikaningsih, Kartikaningsih
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (634.918 KB) | DOI: 10.21776/ub.rjls.2014.001.01.5

Abstract

The effect of Lactobacillus acidophilus microcapsule which encapsulated by kappa-caragenan toward in vivo functional test has been carried out. The goal were to get the viability of L. acidophilus and E. coli in the stool test animals which fed by L. acidophilus encapsulated kappa-caragenan, to  get anti-cholesterol ability  in feces test animals  which fed by  L. acidophilus encapsulated  kappa- caragenan, gain the ability to decrease the activity of b - glukoronidase found in feces test animals by administration of L. acidophilus which encapsulated by kappa-carrageenan, get the ability of L. Acidophilus which encapsulated by kappa-carrageenan as an inducer of IgA obtained from stool test animals. Conclusion were  the administration L. acidophilus encapsulated by kappa- caragenan may improve the viability of L. acidophilus and E. coli feces of mice and can raise cholesterol levels feces of mice and decrease the activity of b - glukoronidase feces of mice and increase levels of fecal IgA mice.
Engineering Technology for Increasing Soybean Production and to Control Damping-off Disease Using Biological Fertilization Mixture Sastrahidayat, Ika Rochdjatun
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (718.59 KB) | DOI: 10.21776/ub.rjls.2014.001.01.3

Abstract

Fall seedling diseases (damping-off) caused by Sclerotium rolfsii is one factor in low productivity of soybean in Indonesia, which may cause loss results achieved 30-75% depending on the level of attacks. Given the character of soil borne diseases (soil borne), then all control efforts through many obstacles, either because the pathogen can survive for decades in the form resistant propagules (Sclerotium) although there is no host for the development.This study aimed to find ways to control the disease by utilizing the potential of natural biological materials and find ways to get control for effective results. For this purpose the various steps of research has been conducted both in laboratory and greenhouse using experimental research design (completely randomized design) and F test to see the difference. The results show that in the first year of research proven to provide results tuba root extract is effective in controlling pathogens. Streptomyces isolates proved to be antagonistic to Sclerotium rolfsii, and when combined with mycorrhizae (AM) will be able to increase its effectiveness. Introduction of inoculum will likely increase their effectiveness when mixed directly into cropping land that can be done as a biological fertilizer just before planting. There is a tendency that the combination treatment between biopesticide will help the effectiveness of its control, though not necessarily affect the production, so the increase in soybean production is due to the protection of soybean from the dead early in the seedling that can suppress up to 50 percent compared with the control.
Estimation of Diverse Porang (Amorphophallus Muelleri Blume) Age in Forest Are Based on Brancing Pattern of Leaf Petiolule Harijati, Nunung; Mastuti, Retno
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (783.626 KB) | DOI: 10.21776/ub.rjls.2014.001.01.4

Abstract

Porang is higher plant which has unique morphology. Tuber, petiole and lamina are the main part of its body. Even Porang doesn’t have true stem, only petiole, its lamina not simple leaves but compound leaves with special pattern. Branching patterns of petiolule are not same in different age. Therefore the aim of research was to observe branching pattern of petiolule from Porang age 1-4 that lived in their native habitat i.e. forest. The research was conducted in secondary forest in Sumberbendo village, Madiun.  Determination porang age was helped by expert farmer that worked with porang for long time. The result showed that Porang age 1 had petiolule with braching type 1-0. Porang age 2 was 1-3, Porang age 3 was 1-3-2, and Porang age 4 was 1-3-2-2. The petiolule which acted as a base of new branch had one or few single simple leaf. The leave could be both sinus and un-sinus leaves located in between two initial or base branching. The position of the leaves were opposite or alternate along with petiolule. If location single leaf just in point branching, the new petiolule morphology was not be considered as petiolule but midrib.
Using Wheat Flour and Alginate In Mozzarella Cheese Making On Physical and Sensory Quality Purwadi, Purwadi; Manab, Abdul
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.021 KB) | DOI: 10.21776/ub.rjls.2014.001.01.7

Abstract

The purpose of this research was to know the best combination of using wheat flour and alginate on physical and sensory quality of Mozzarella cheese.The method that used in this research was factorial experiment with completely randomized design by using twelve treatments and three times repeatation. The variable measured was hardness, cutting point and sensory. The obtained data was analized by using analysis of variance continued by honesty significant difference (HSD).The result of this research showed that the used of wheat flour and alginate did not give a significance different interaction (P > 0,05) on the average of hardness, cutting point and sensory quality (colour, texture, and taste) of Mozzarella cheese,  but used of wheat flour give a different significance effect (P < 0,05) on hardness and cutting point of Mozzarella cheese. The highest value was the treatment of T4A3, that was the combination of 7,5 % wheat flour and  1 % alginate.
α-Amylase and α-Glucosidase Inhibition by Brown Seaweed (Sargassum sp) Extracts Firdaus, Muhammad; Prihanto, Asep Awaludin
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.827 KB) | DOI: 10.21776/ub.rjls.2014.001.01.2

Abstract

Inhibition of intestinal α-amylase and α-glucosidase is an important strategy to control post-prandial hyperglycemia associated with diabetes mellitus. In vitro inhibitory effects of crude extracts of seaweed against α-amylase and α-glucosidase were studied. Crude ethyl acetate extracts of Sargassum aquifolium were the stronger inhibitor to α-amylase and α-glucosidase than others. Furthermore, Sargassum aquifolium ethyl acetate extract significantly suppressed the rise in postprandial glucose level after oral administration of glucose in normal rats. The results of this study suggest that the crude Sargassum aquifolium extract may suppress the rise in postprandial hyperglycemia in vivo in part, through inhibition of alpha amylase and glucosidase.
Marker Spesifik Combine DNA Index System (CODIS) 13 dalam Identifikasi Forensik pada Suku Jawa dan Madura di Indonesia Prastowo, Wening; Listiani, Fitria Dewi
Research Journal of Life Science Vol 1, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.674 KB) | DOI: 10.21776/ub.rjls.2014.001.01.1

Abstract

Forensic identification can be performed DNA examination. FBI had recommended, forensic DNA examination to used short tandem repeat CODIS 13. Result of determine Short tandem repeat CODIS 13, showed specific pattern. Java tribes are shown, the Heterozygousity Index ranged from 0,60069 (VWA) to  0.93752 (D18S51), the Power of Exclution ranged from 0.30885 (VWA), to 0.83068 (D18S51), the Power of Discrimination ranged from 0.52853 (VWA), to 0.99127 (D18S51). Madura tribes shown, the Heterozygousity Index ranged from 0,63021 (VWA) to 0.94445 (FGA), the Power of Exclution ranged from  0.33418 (VWA), to 0.84779 (FGA), the Power of Discrimination ranged from  0.60501 (TPOX), to 0.99305 ( FGA).

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