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Research Journal of Life Science
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Articles 10 Documents
Search results for , issue " Vol 4, No 1 (2017)" : 10 Documents clear
Aqueous Morinda citrifolia Leaves Extract Enhancing Glutathione Peroxidase Activity and α2-Macroglobulin Gene Expression on Macrobrachium rosenbergii Halim, Atika Marisa; Prajitno, Arief
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (774.904 KB) | DOI: 10.21776/ub.rjls.2017.004.01.4

Abstract

Morinda citrifolia, known commercially as noni is often used for enhancing immunity, these plant-rich phenolic compound with antioxidant properties. In the present study, Macrobrachium rosenbergii were fed diets containing aqueous M. citrifolia leaves extract (AMLE) at 0.6, 4 and 6 g kg-1. Glutathione peroxidase (GPx) and α2-macroglubulin (α2-M)  activity were conducted to measure an immune parameter, which was evaluated before and after 7, 21, 35, 49 and 63 days of feeding trial. The results showed that after 63 days of feeding treatment, significantly increased in GPx activity. Moreover, the gene expressions of α2-macroglubulin was significantly upregulated. These results recommend that administration of AMLE can be used as an immunostimulant and regulated immune response and immune gene expression in M. rosenbergii. 
Characteristics of Beach Seine Fishery of East Java: Facing Ministerial Decree of Marine Affairs and Fisheries No. 2/2015 Wiadnya, Dewa Gede Raka; Wiryawan, Budy; Marsoedi, Marsoedi; Kusuma, Wahyu Endra; Darmawan, Arief
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.48 KB) | DOI: 10.21776/ub.rjls.2017.004.01.9

Abstract

Catch biomass and time-trend perception of Beach seine fishery were studied during 2015, from four different sites in East Java: Padikde Madura, Santan Island Banyuwangi, Prigi Beach Trenggalek, and Dangkal Beach Pacitan. The study aimed to assess the impact of recently Ministerial Decree (No. 2/2015) on this particular fishery. Catch biomass was analyzed based on swept area method. Time trend perception was traced back from fishermen in relation to reduced catch biomass, catch size, habitat degradation, and income contribution from the fishery. The results showed that Beach Seines in East Java are considerably subsistence. It swept fixed-bottom habitats of 1–9 ha. Average catch biomass for all sites was 15±19.4 kg trip-1. With low price of fish catch, fishermen cannot place down their family income solely from this livelihood. Beach seine is more as to maintain tradition of subsistence. Fishermen clearly observed the reduced in catch biomass and catch size compared to what it used to be (±30 years ago). With very low contribution to total catch in the region, negligible impact to habitat degradation, and consumed no fuel subsidy, it is no real reason to include this gear in general ban through Ministerial Decree of Marine Affairs and Fisheries.
Survey of Hawksbill Turtle (Eretmochelys imbricate) Health Condition in Terms of Parasites and Microbes in Alas Purwo National Park, Indonesia A'yunin, Qurrota; Nursyam, Happy; Andayani, Sri; Maftuch, Maftuch; Maftuchah, Lukluk Intan
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.704 KB) | DOI: 10.21776/ub.rjls.2017.004.01.5

Abstract

Indonesian waters have six types of turtles that can live, spawn and breed. Sea turtle conservation becomes an important and urgent program to be done in order to protect and save sea turtle population in Indonesia. One of the factors that most affect the turtle population is the cause of degradation of pathogenic factors. Alas Purwo National Park, East Java, there is some communities that have activities turtle conservation. Conservation is done by securing and protecting turtle eggs. Turtle eggs that have hatched are released into the sea once it is ready. This study aims was to determine the type of bacteria and fungi that infect hatchlings and environmental factors that influence. This research is descriptive method to Hawksbill turtle (Eretmochelys imbricate) is by way of random sampling. Sampling of microbes in sea turtle was conducted using cotton swab method and then microbes was cultured and indentified in laboratory. The results showed The kind of parasites and microbes which were indentified in hatching and adult Hawksbill sea turtles were fungus with genus Aspergillus sp., Geotrichum sp., Fusarium sp., and Gliocladium sp. ; bacteria are Pseudomonas aeruginosa and Enterobacter cloaceae; and parasite is Chelonibia testudinaria barnacles.  The parameter average value of water in pond indicated 28.1 – 29.2°C for temperature, 32 - 34 ‰ for salinity, 7.78 – 8.2 for pH, and 3.86 – 4.21 mg/L for DO. 
Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat Rohman, Mohammad Saifur; Lukitasari, Mifetika; Nugroho, Dwi Adi; Nashi, Widodo; Nugraheini, Nur Ida Panca; Sardjono, Teguh Wahyu
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.041 KB) | DOI: 10.21776/ub.rjls.2017.004.01.10

Abstract

The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.
Application of Optimal Control Strategies for the Spread of HIV in a Population Marsudi, Marsudi; Hidayat, Noor; Wibowo, Ratno Bagus Edy
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.253 KB) | DOI: 10.21776/ub.rjls.2017.004.01.1

Abstract

This paper presents an application of optimal control theory to assess the effectiveness of control measures on the spread of HIV in a population.  This paper formulates and analyzes a deterministic mathematical model with use of condom, screening and therapy as control variables using optimal control theory and Pontryagin’s Maximum Principle. It formulates the appropriate optimal control problem and investigate the necessary conditions for the disease control in order to determine the role of unaware infectives in the spread of HIV using of condom, screening of unaware infective and antiretroviral therapy are used as the control items. The optimality system is derived and solved numerically.
Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Susianti, Hani; Barlianto, Wisnu; Hanggara, Dian Sukma; Handono, Kusworini; Adipireno, Purwanto; Suromo, Lisyani
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.828 KB) | DOI: 10.21776/ub.rjls.2017.004.01.6

Abstract

Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN. 
The Effect of Niosomal System (Span 60-Cholesterol) on Diclofenac Sodium Preparation Characteristics and Diclofenac Sodium Preparation of Hydroxypropyl Cellulose Gel Base (HPC) Danimayostu, Adeltrudis Adelsa; Hendradi, Esti; Purwanti, Tutiek
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.031 KB) | DOI: 10.21776/ub.rjls.2017.004.01.2

Abstract

Diclofenac sodium is a lipophilic drug. That characteristic makes it difficult to disperse well in hydrophilic gel base. The niosome with its vesicle system could overcome that low dispersibility. It affects penetration by reducing water loss in trans-epidermal layer and change lipid bilayer conformation. The present study was designed to investigate the effect of niosomal system (Span 60–Cholesterol) on preparation characteristics and diclofenac sodium penetration of hydroxypropyl cellulose (HPC) gel base. We examined three different formulas in HPC gel base. Formula III was made in niosomal system.  Preparation  characteristics were  evaluated with organoleptic and acidity tests. Drug penetration was checked using apparatus 5-paddle over disk and 0.45 μm Milipore membrane impregnated with isopropyl myristate. The solution is phosphate buffer saline pH 7.4±0.05 in temperature 37±0.5°C. All of the data were evaluated based on one way ANOVA and continued with HSD test. It was concluded that niosomal system (span 60-cholesterol) has an influence in increasing pH value and penetration (based on flux value and permeability) of diclofenac sodium in HPC gel base.
Eficacy of Water Clover Extract (Marsilea crenata) Against Blood Estrogen Progesteron Balance, Blood Calcium Levels and Impact on Dense of Bone Tissue of Rat (Rattus novergicus) Trisunuwati, Pratiwi
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (736.216 KB) | DOI: 10.21776/ub.rjls.2017.004.01.7

Abstract

The potential of phytoestrogen for the stabilized of low condition blood estrogen hormone much more important to be sudy. So the aim of this reseach were to find an natural herbal active compounds Water Clover Extract (Marsilea crenata) which contain phytoestrogen isoflavones as an alternative invasive treatment against menopause women and subestrus in dam of livestock. The other advantage is that could be potential to develop as alternative compound against animal with low level of blood calcium that relevance on progesterone level, because there was some rising of exogen phytoestrogen. This research is true experimentally post test control design based on completely randomized design. The old females white rat (Rattus norvegicus), divided into 5 groups challenges with 2 ml each Marsilea crenata extract in different concentration such as 6.25%, 12.5%, 25%, 50% given by gastric sonde along 23 days, compare negative control group. Level of blood calcium using SSA showed increase in all the treatment (P<0,05)  started on 12,5%, lower the progesterone and raised estrogen hormone as natural balance. To find about effect of Calcium toward osteogenesis used to determine the description of histopathologis (HE) bone tissue that impact on bone density. Histology os humerus showed that have impact on dense of bone tissue. The conclusion of this research were Marsilea crenata juice plays a role in increasing toward blood calcium levels and surely increase bone density in animal laboratory (Rattus norvergicus), supporting by blood  estrogen progesterone  balance. The suggestion was Marsilea crenata extract could be potential as a substance to increase estrogenic progesterone balance and rise blood calcium both in human and animal.
Enzymatic Cellulose Palmitate Synthesis Using Immobilized Lipase Roosdiana, Anna; Mardiana, Diah; Indahyanti, Ellya; Oktavianie, Dyah Ayu
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.029 KB) | DOI: 10.21776/ub.rjls.2017.004.01.3

Abstract

Bacterial cellulose can be modified by esterification using palmitic acid and Mucor miehei  lipase  as catalyst. The purpose of this research was to determine the optimum conditions of esterification reaction of cellulose and palmitic acid . The esterification reaction was carried out at the time variation  of  6, 12, 18, 24 and 30 hours and the mass ratio of cellulose: palmitic acid (1: 11: 2, 1: 3, 1: 4, 1: 5,1:6) at 50 °C. The   cellulose palmitate  was examined  its  physical and chemical properties by using FTIR spectrophotometer, XRD, bubble point test and saponification  apparatus. The results showed that the optimum reaction time of esterification reaction of cellulose and palmitic acid occurred within 24 hours and the mass ratio of cellulose: palmitic acid was 1: 3 resulting in DS of  0.376 with  swelling index of 187 %, crystallinity index of 61.95%,  and Φ porous of 2.40 μm. Identification of functional groups using FTIR spectrophotometer showed that C=O ester group  was observed at 1737.74 cm-1 and strengthened  by  the appearance of C-O ester peak at 1280 cm-1. The conclusion of this study is reaction time and reactant ratio influence significantly the DS of cellulose ester. 
Development of Candidate Antigens for Rapid Test Kit to Detect Autoantibodies in Patients with Systemic Lupus Erythematosus Barlianto, Wisnu; Susianti, Hani; Wahono, Singgih; Ismayasih, Nelly; Meilani, Rossy; Handono, Kusworini
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.941 KB) | DOI: 10.21776/ub.rjls.2017.004.01.8

Abstract

Systemic Lupus Erythematosus (LES) is an autoimmune inflammatory disease characterized by the formation of anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modified-manual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.

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