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Research Journal of Life Science
Published by Universitas Brawijaya
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Articles 8 Documents
Search results for , issue " Vol 4, No 2 (2017)" : 8 Documents clear
Accumulation Time and Antioxidant Responses of Inorganic Arsenic on Brown Seaweed Padina minor Violando, Wiga Alif; Andayani, Sri; Wong, Saou-Lien; Tseng, Chih-Hsuan
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.843 KB) | DOI: 10.21776/ub.rjls.2017.004.02.4

Abstract

Padina minor had a good ability to bind and accumulate arsenic (As). This research aimed to observe the As accumulation and detoxification time, also the antioxidant defense activities. Each gram of P. minor was culture in various As contaminate media for 7 days, then reculture in As-free media for 14 days. There was an acute effect on thali by decreasing its growth slowly till final day with small amount accumulation at concentration 25 µg iAs g-1. Meanwhile, direct attenuation impact was due at 50 and 100 µg iAs g-1 with massive accumulation. The thali starts to recover after reculture in health environment for 14 days. Only As5+ was detected on the thali at day 7 and 21 were indicates internal oxidation of As3+ was done before 7 days. Various antioxidant activities such as decreasing polysaccharide and increasing activities of DPPH and flavonoid at high level were observed. Those indicated that 50 µg iAs g-1 was optimum iAs level on P. minor. The results indicate that P. minor has ability to oxidase and methylate with antioxidant role as defensive activities against iAs, but it also needs more than 14 days to recover in high level contamination.
Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates Safitri, Nur Maulida; Herawati, Endang Yuli; Hsu, Jue Liang
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.285 KB) | DOI: 10.21776/ub.rjls.2017.004.02.5

Abstract

The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.
The Effect of Used Form and Level Green Cincau Leaves (Cycleabarbata L. Miers) as Feed Additive on Broiler Performance Production Natsir, Muhammad Halim; Sjofjan, Osfar; Muharlien, Muharlien
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (841.323 KB) | DOI: 10.21776/ub.rjls.2017.004.02.1

Abstract

The aimed research was to examine the effect of used form and level green cincau leaves (Cycleabarbata L. Miers) as feed additive on broiler performance (feed consumption, body weight gain, feed conversion ratio and income over feed cost). The materials used were 192 Day Old Chicks (DOC) of Lohman MB Platinum with average initial body weight of 37.4±2.87 g, encapsulated or non encapsulated green cincau leaves (Cycleabarbata L. Miers) and encapsulants. Encapsulants that used was the mixture of gum arab-whey (4:1) wich was added BHT 0.06%. Encapsulants that used was 25% of green cincau leaves (Cycleabarbata L. Miers). The method in this experiment was in vivo experiment with Nested Completely Randomized Design with 2 form of green cincau leaves (Cycleabarbata L. Miers) (non encapsulated and encapsulated) and 4 levels of inclusions 0, 0.5, 1.0 and 1.5%, if there were significant effect it would be future tested with Duncan’s Multiple Range Test. The result showed that green cincau leaves (Cycleabarbata L. Miers) in either encapsulated and non encapsulated form didn’t significantly effect (P>0.05) on consumption, feed conversion ratio and IOFC, but it significantly affected (P<0.05) body weight gain and they tended to increase (P<0.01) production index. Increasing levels of green cincau leaves (Cycleabarbata L. Miers) form 0 to 1.5% in encapsulated and non encapsulated form didn’t significantly improve feed conversion ratio, IOFC and production index, thought they tended to increase (P<0.05) feed consumption and they tended to increase (P<0.01) body weight gain. The conclusion of this research was encapsulated better than non encapsulated green cincau leaves (Cycleabarbata L. Miers). The optimal level of feed additive in encapsulated green cincau leaves (Cycleabarbata L. Miers) was 1.0%.
The Analysis of Pandanus Relationship of Purwodadi Botanical Garden Collections Based on Morphological Character and Molecular Marker (trnL and trnL-F) Rachma, Risca Adiyani; Hendrian, Hendrian; Azrianingsih, Rodiyati
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (948.933 KB) | DOI: 10.21776/ub.rjls.2017.004.02.6

Abstract

The morphological characteristics of plant vegetative organ has the potential to be the defining factor of pandanus family collection at Purwodadi Botanical Garden (PBG). However, the information is not enough to confirm the identity of pandanus at the type-level. This problem happened at the collection of PBG. It is an urgent issue considering PBG as one of the most important ex-situ plant conservation institute that also has function as a study source for plant taxonomy science in Indonesia. Therefore, the use of molecular technology analysis using molecular marker (primer) is incredibly important to identify the member of pandanus family by identifying its family relationship pattern. This information was expected to give a clarity about the identity of Pandanus family at PBG which will support further research and activity for pandanus conservation. The plant sample includes 16 specimens of pandanus with one member of the family from Freycinetia as the outgroup. Morphological analysis of the 50 characteristics of vegetative organs (leaf, stem, and root) was conducted. Molecular analysis of DNA using primer of trnL and trnL-F. The data sequence as a result of the analysis was then tested using homology test with BLAST. All of the DNA sequence was paralleled using Clustal W analysis, the tree construction relationship was analysed using Maximum Parsimony (MP) method with MEGA 6.0 program. The results of morphological studies showed the character vegetative that is the position of the spines on the leaves, the shape of apex leaf and the condition of the root surface above the soil. Molecularly, appears 1 new genera that has not been previously identified by PBG researcher is Benstonea.
Chemical Characteristics of Biscuit Substituted by Modified Cassava Product (Mocap) Flour from High Cyanide Variety of Cassava Harijono, Harijono; Pulungan, Maimunah Hindun; Muchlisyiyah, Jhauharotul; Tanti, Erna Triyas; Estiasih, Teti
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.257 KB) | DOI: 10.21776/ub.rjls.2017.004.02.2

Abstract

Cassava (Manihot esculenta Cranzt) is easily cultivated and an important source of carbohydrates. Currently, only a certain type of low cyanide cassava has been utilized for food product and intermediate product as flour. Processing of high cyanide cassava through submerged and solid-state fermentation simultaneously into mocap (modified cassava product) flour has successfully lowered the cyanide into a safe level for consumption. The application of mocap flour for biscuit is important to know the characteristic changes that occur due to substitution of mocap flour. Variations of wheat flour: mocap flour of 40:60, 30:70, 20:80, 10:90, and 0:100 were used in the preparation of biscuits. The resulting biscuits were analyzed for their chemical characteristics including moisture, protein, fat, starch, amylose, crude fiber, reducing sugars, and cyanide compounds. The cyanide compounds comprised of total cyanide, HCN, and cyanogenic glycosides.  Substitution of wheat flour by mocap flour in biscuit making produced the changes of biscuit characteristics. Protein, moisture, starch, amylose, fat, and reducing sugar content was decreased by increasing substitution of mocap flour. Meanwhile, crude fiber and cyanogenic compounds increased by increasing mocap flour substitution. All biscuits with variation of mocap flour substitution were safe to consume because of low level of cyanides.
Use of Six Planting Media on Shallow Bed and Plant Container Systems to The Growth and Yield of Sweet Potato (Ipomoea batatas L.) for Roof Garden Sitawati, Sitawati; Suryanto, Agus; Nurlaelih, Euis Elih
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.123 KB) | DOI: 10.21776/ub.rjls.2017.004.02.7

Abstract

Roof garden is an alternative to overcome food security through breeding crops and horticultures of fruits and vegetables. The research was conducted in Malang by applying two planting systems, such as shallow bed (direct cropping) and plant container bag by the same planting medium treatments. Research, which applied shallow bed and container bag, was conducted using Randomized Block Design (RBD) by 6 treatments of planting medium and 4 replications, such as : Soil (100%) (M0), Soil + Charcoal (1:1) (M1), Soil + Cocopeat (1:1) (M2), Soil + Compost (1:1) (M3), Soil + Manure (1:1) (M4), and Soil + Moss (1:1) (M5). Data analysis of the research used analysis of variance, and if any significant influence was found, it would be followed by the Least Significant Difference (LSD) at level 5%. And then, it would be followed by T-test as comparison between shallow bed system and container bag system. Parameters that being observed in the research included length of crop, leaf area, numbers of tuber, fresh weight of tuber, dry weight of tuber, and total dry weight of plants. Results of the research showed that the application of medium types of soil and manure (1:1) by shallow bed system on Roof Garden concept may increase numbers of tuber, fresh weight of tuber, dry weight of tuber, and dry weight of Sweet Potato (Ipomoea batatas L.) in comparison with the application of soil medium and manure (1:1) by plant container bag system.
The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding Ciptadi, Gatot; Ihsan, Muhammad Nur; Rahayu, Sri; Nurgiartiningsih, Veronica Margareta Ani; Mudawamah, Mudawamah; Putri, Ardyah Ramadhina Irsanti
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.513 KB) | DOI: 10.21776/ub.rjls.2017.004.02.3

Abstract

Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38°C. Colcichine was added after 70 hours and kept for 2–3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. It was observed that all cattle tested in this research were normal categories.  The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome.
Polymorphism of Growth Hormone Gene in Selecting Etawah Crossbred (PE) Goats Susilorini, Tri Eko; Kuswati, Kuswati; Maylinda, Sucik
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.749 KB) | DOI: 10.21776/ub.rjls.2017.004.02.8

Abstract

Although Etawah Crossbred (PE) goat is considered to be dual purpose (meat and milk) goat, it is mainly raised for meat production. Since early 1990, there has been a growing interest of the farmer in some places to raise PE goat for milk production without sacrificing its role to produce kids for meat. Although milk yield of PE goat was not as high as milk yield of some other dairy goats, the ability of PE goat to cope with harsh local environment, particularly climate and feed conditions, was an advantage. Therefore, raising PE goat would still be an important part of farmer activities in the rural areas in Indonesia. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. The objective of this study was to detect the genetic polymorphism of GH gene in major Etawah Crossbred (PE) goat using PCR-RFLP. The PCR amplified fragment were digested with HaeIII endonuclease and the result showed the presence of two genotype CC and CD. The total frequency were 47.0% and 53.0% for CC and CD genotype respectively in 94 tested goats. Statistical analysis showed that in the fragment amplified by the pair of  primer, CD genotype had significant higher birth weight and weight of 100 days old (weaning weight) than CC genotype (P<0.01). In conclusion that GH gene may be a mayor gene or linked to the mayor gene to affect the weight traits and the polymorphic site could be used to select the goat weight in marker-assisted selection program.

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