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Research Journal of Life Science
Published by Universitas Brawijaya
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Articles 8 Documents
Search results for , issue " Vol 4, No 3 (2017)" : 8 Documents clear
Effect of Cinnamaldehyde Addition on Zebrafish (Danio rerio) Eggs Against Streptococcus agalactiae Infection Puspitasari, Asthervina Widyastami; Arfiati, Diana; Hu, Shao-Yang
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (359.334 KB) | DOI: 10.21776/ub.rjls.2017.004.03.5

Abstract

In this study, the effect of cinnamaldehyde (CA) towards Streptococcus agalactiae on zebrafish egg were investigated. To obtain the optimal concentration, CA and S. agalactiae were tested by short-term effects assay. Fresh zebrafish egg, CA and S. agalactiae were observed in different groups, single and mix treatment. The result showed that the larval survival rate (%) of single CA concentration (2.5 and 5 µg mL-1) were not significantly different compared to the control, with the value 94 ± 2.8% and 93 ± 3.3% respectively. Meanwhile, the single S. agalactiae concentration (109 and 1010 CFU mL-1) were significantly different in comparison to the control with the value 73 ± 2.1% and 6 ± 2.5%. Mix groups, larval survival rate (%) value were significantly different with the best combination value was 17 ± 4.7% at 5 µg mL-1 of CA and 109 CFU mL-1 of S. agalactiae. Even though CA may potential for zebrafish egg shelter against S. agalactiae infection. But a mixture (CA and S. agalactiae) treatments were not recommended for this study because the low value was shown compared to the single treatments. This possibility may occur because of the debris formation of two substances which leads to the poor environment and causes to low fish larval survival rate (%) value. For further study, the water quality assay is needed.
Black Grass Jelly (Mesona Palustris Bl) Effervescent Powder has Anti-Dyslipidemia in High Cholesterol Diet-Fed Rats and Antioxidant Activity Handayani, Dian; Widyaningsih, Tri Dewanti; Wijayanti, Novita; Etika, Mey
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.057 KB) | DOI: 10.21776/ub.rjls.2017.004.03.1

Abstract

Dyslipidemia that could be affected by hypercholesterol diet is a contributing factor in millions of deaths worldwide and a risk factor for ischemic heart disease and stroke. High soluble fiber diet is used as one of the non-pharmacological treatments to control hypercholesterolemia. Black Grass Jelly (Mesona palustris BL) also known as black cincau has a high soluble fiber content. This study aimed to investigate the effect of black cincau as effervescent powder form (BCEP) for anti-dyslipidemia and also the antioxidant activity. BCEP was orally administered to twenty male dyslipidemia Wistar rats induced with hypercholesterol diet for 3 weeks then plasma lipid profile was measured using enzymatic CHOD-PAP assay. The antioxidant activity also has been identified. Oral administration of 3 different doses of BCEP resulted in lipid profiles by decreasing plasma cholesterol (TC), triacylglycerol (TG), low-density lipoprotein (LDL), and also high-density lipoprotein (HDL) in dyslipidemia rats (α =  0.01). The increasing doses of BCEP revealed that it has a potential effect of decreasing plasma lipid.
Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats Nugrahenny, Dian; Permatasari, Nur; Rohman, Mohammad Saifur
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.898 KB) | DOI: 10.21776/ub.rjls.2017.004.03.6

Abstract

The administration of deoxy-corticosterone acetate (DOCA)-salt can induce oxidative stress leading to decrease the bioavailability of nitric oxide (NO), increase senescence of circulating endothelial progenitor cells (EPCs), thus contributing to endothelial dysfunction. This study was aimed to investigate the effects of Physalis minima L. leaves extract on serum NO levels, circulating EPCs number, and histopathology of tail artery endothelial cells in DOCA-salt-induced endothelial dysfunction in rats. Twenty-five male Wistar rats were randomly divided into five groups: rats without any treatment (normal), rats treated with DOCA (10 mg/kgBW s.c. twice weekly) and given 0.9% NaCl to drink ad libitum for 6 weeks, and DOCA-salt-induced rats orally supplemented with P. minima leaves extract at doses of 500, 1500, or 2500 mg/kgBW for 4 weeks. Serum NO levels were measured by colorimetry. The number of circulating EPCs (CD34+/CD133+ cells) was determined by flow cytometry. The tail artery sections were histologically processed with hematoxylin-eosin staining. DOCA-salt-induced rats showed significantly (p<0.05) decrease in serum NO levels and circulating EPCs number compared to the normal. There was also more detached tail artery endothelial cells in DOCA-salt-induced rats. P. minima leaves extract at a dose of 500 mg/kgBW significantly (p<0.05) increased serum NO level and circulating EPCs number, and also induced an optimal re-endothelialization in DOCA-salt-induced rats. P. minima leave extract dose-dependently increases NO bioavailability contributing to enhanced EPCs mobilization, thereby promoting re-endothelialization in DOCA-salt-induced endothelial dysfunction in rats.
The Effect of Different Seasons and Land Types Towards The Performance of Bali Cattle Production in Southeast Sulawesi Wangi, La; Busono, Woro; Nasich, Moch
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (743.867 KB) | DOI: 10.21776/ub.rjls.2017.004.03.2

Abstract

The Bali Cattle in Southeast Sulawesi its productivity is influenced by the seasonal environmental conditions. The objective of this study was to analyze the performance production of cows Bali Cattle and its calves that were born on different season (rainy season and dry season) as well as on different types of land. The research methods applied were survey and observation method. The variables being observed follows: body weight (BWT), daily body weight gain (PBBH), body condition score (BCS) and birth weight (BW). Data were analyzed by using the Analysis of Variance (ANOVA) of nested design. The result of the research indicated that the average of body weight, daily body weight gain, BCS and birth weight was higher during the rainy season compared to the dry season. The average of body weight, daily body weight gain, and BCS was higher on paddy field. On the contrary, the birth weight on dry area was higher than it was on wetlands. The average of male cattle birth weight was higher than the average of female cattle. It could be concluded that the cow’s working performance production was affected by the feed consumption and environments (season and the type of land). On the other hand, calf birth weight was affected by sexes, in addition it was also related to cow’s feed intake on late pregnancy phase.
Determination Amylolitic Characteristic of Predominant Lactic Acid Bacteria Isolated during Growol Fermentation, in a Different Starch Medium Composition Putri, Widya Dwi Rukmi; Zubaidah, Elok; Masahid, Ardiyan Dwi
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.258 KB) | DOI: 10.21776/ub.rjls.2017.004.03.7

Abstract

In order to achieve efficient lactic acid production from starch, fermentation of avarious composition starch medium by lactic acid bacteriawas examined in this study. Many strains of Lactobacillus plantarum isolated from growol fermentation, Lactobacillus plantarumsubsp. plantarum NBRC 15891 and Lactobacillus amylophyllus NBRC 15881 were used as starter cultures in starch basis medium, i.e, basal, basal-starch, enriched basal-starch with polypeptone and yeast extract. Lactobacillus plantarum UA3, AA2, AA11 showed the highest cells growth compare to both reference strains, but Lactobacillus amylophyllus NBRC 15881 showed a greater ability to degrade starch indicated by decreasing of pH and starch content of the fermented substrate. Enriched medium with peptone and yeast extract could generate the growth and starch degradation capabilities for all types of lactic acid bacteria were used.
Post Thawing Sperm Quality and Ca+2 Intensity Characters of Local Goat Sperm After Freezing by Simple Method Using Deep Freezing Ciptadi, Gatot; Ihsan, Muhammad Nur; Rahayu, Sri; Wahjuningsih, Sri; Munazaroh, Anis Mei; Putra, R. P.
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.085 KB) | DOI: 10.21776/ub.rjls.2017.004.03.3

Abstract

The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a post-thawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of -45°C then -196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca+2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca++ intensity was analysis descriptively. The result showed that post-thawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at -196°C. However freezing sperm conserved in -196°C is better than in -45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca+2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 µm each.  It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.
Ethanol Fermentation Potency of Wild Yeast Which Isolated From Soil Drive Nutrient (Sdn) Plantation System Muhibuddin, Anton; Ivanesthi, Indira Rizqita; Nurhatika, Sri; Basuki, Tjuk Eko Hari
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.983 KB) | DOI: 10.21776/ub.rjls.2017.004.03.8

Abstract

Yeast which has a fermentative type can ferment alcohol, which break down the sugars into alcohol, lactic acid and gas. This yeast’s type is needed in biofertilizer process production. The purpose of this study was to observed the potential in ethanol production of the soil wild yeast that isolated from Jember-East Java, and determine the concentration of sugar product as well as the best time of ethanol fermentation. Our previously observation have found 113 yeast species. Then we select them based on fermentative ability and found five best species. These five species then has determined it’s sugar product as well as the best time of ethanol fermentation and choose two best. We have counted the level of ethanol production using glucose medium of 10%, 15% and 20% of these two best species. The results showed that Candida sp. 1 (species number-1) and Candida sp. 3 (species number-3) has a better potential to produce ethanol. The highest ethanol content was produced by Candida sp. 1 (56%) while by Candida sp. 3 (70%). While the optimum time for the ethanol fermentation process Candida sp. 1 and Candida sp. 3 is 24 hours.
The Effects of Non-Genetic Factors on The Birth Weight, Litter Size and Pre-Weaning Survive Ability of Etawah Cross-Breed Goats in The Breeding Village Center in Ampelgading District Susilorini, Tri Eko; Kuswati, Kuswati; Maylinda, Sucik
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.882 KB) | DOI: 10.21776/ub.rjls.2017.004.03.4

Abstract

A total of 106 late gestation goats (age 4-5 months of pregnancy) records each of Etawah Crosbred goats kept on small farmer over a period of 10 years were assessed to determine the effects of environmental factors on them. Body Condition Score is non-genetic factor and in dairy goats to predicted milk production, fertility, and general health of the animal because BCS is visualisasi from feeding manajement. The objective of this study was to investigated the relationship between Body Condition Score (BCS) of late gestation with litter size and birth weight on etawah crossbred goats. The results showed that the average of litter size and birth weight (kg) were 1.89±0.66 and 3.84±0.73 respectively. The relationship between BCS with litter size were 0.13 (very low) and the relationship between BCS with birth weight were 0.11 (very low). The conclusion of this research is BCS of late gestation had positive correlation on litter size and birth weight, however this was low and birth weight as first indicator of future growth rate.

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