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Research Journal of Life Science
Published by Universitas Brawijaya
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Articles 7 Documents
Search results for , issue "Vol 6, No 2 (2019)" : 7 Documents clear
Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda Rusmawanto, Rusmawanto; Prajitno, Arief; Yuniarti, Ating
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.624 KB) | DOI: 10.21776/ub.rjls.2019.006.02.1

Abstract

Dunaliella salina is a type of marine microalgae. The objective of this research were investigated the effect of D. salina to inhibit the growth of E. tarda. The method used in this study was examined antibacterial activity of D. salina using disc diffusion and co culture test. The secondary metabolite compound in D. salina was tested using phytochemical screening and FTIR methods. The results obtained D. salina extracted using n-hexane showed the best activity for inhibiting the growth of E. tarda. Minimum concentration of 100 mg.L-1 crude extract can inhibit E. tarda with a total of bacterial colonies 137x1011 CFU.mL-1. The phytochemical test results showed that D. salina extract using n-hexane contained flavonoids, saponins, alkaloids, terpenoids and phenols. The FTIR analysis showed phenol and carotene compounds are one of the secondary metabolites that can be used as antibacterial. It can be concluded that the extract has strong antibacterial activity against E. tarda and potentially as antibacterial in aquaculture.
Accumulation of Heavy Metals Lead (Pb) and Copper (Cu) in Mangrove Area of Avicennia marina in Manyar Subdistrict, Gresik District, East Java Maharani, Made Dwipa Kusuma; Hertika, Asus Maizar Suryanto; Musa, Muhammad
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.195 KB) | DOI: 10.21776/ub.rjls.2019.006.02.4

Abstract

Human and industrial activities in the area of Manyar Subdistrict Mangrove Waters, Gresik District can increase pollution and the presence of heavy metals in aquatic environments and aquatic organisms. This triggers input of heavy metals, especially Pb and Cu. Mangroves can accumulate and have a high tolerance for heavy metals so they can be used as plants for phytoremidiation purposes (phytostabilization). The mangrove ecosystem can be used as a control of heavy metal pollution and can also be used as a pollutant trap. This study was conducted to determine the potential of phytoremidiation based on the accumulation and translocation of heavy metals in Avicennia marina mangroves in absorbing heavy metals Pb and Cu from their environment. Based on research results MacFarlane et al., (2007) mangrove Avicennia marina is one of the plants that can effectively accumulate heavy metals due to its root system. The sample was analyzed using AAS (Atomic Absorption Spectrophotometer). The research parameters include levels of Pb and Cu metals in sediments, mangrove roots and leaves. The results of Pb heavy metal concentrations ranged from 0.30 ppm - 4.84 ppm and Cu ranged from 0.27 ppm - 11.42 ppm with the highest values found in sediment stations 2. Avicennia marina at the study site was excluder because the BCF value < 1 which ranged from 0.02 - 0.18 ppm and phyto-extraction because the value of TF > 1 ranged from 0.35 to 1.69 ppm. The results of this study indicate that Avicennia marina mangroves can be developed into phytoremidiation agents because they are able to absorb and transfer heavy metals from the environment to other body tissues.
Effect of Sweet Purple Potato Extract (Ipomoea batatas L) on Zebrafish (Danio rerio) by Diet Induced Obesity Nur, Aswaty; Riskawati, Yhusi Karina; Widodo, Edwin; Ratnawati, Retty; Firdausy, Amirah; Dewi, Lydia Kusuma; Karina, Maria
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.637 KB) | DOI: 10.21776/ub.rjls.2019.006.02.2

Abstract

The effect of a high-fat diet on zebrafish as an alternative experimental animal in studying lipid metabolism is not fully understood. In this research, sweet purple potato extract (SPPE) which has anthocyanin have used as an anti-obesity property. Zebrafish aged 8 mpf was divided into five groups: Non-DIO (control negative), DIO (Diet-Induced Obesity, control positive), and three experimental groups (DIO A, DIO B, DIO C). Each group consisted of five Zebrafish. All groups have been fed freshly hatched Artemia for 40 days where the Non-DIO?s amount was 5 mg Cyst/fish one time per day, and the other groups? amount was 60 mg Cyst/fish three times per day. The experimental groups were exposed to sweet purple potato extract three times per day by different SPPE doses: DIO A by 80 ppm, DIO B by 120 ppm, and DIO C by 160 ppm. The results show that there was no significant difference between the positive control group and the experimental groups in term of feeding intake, BMI, blood glucose, and total cholesterol. However, based on molecular parameters, there was a declining trend on PPAR-? level, although, the adiponectin level remained stable. We suggest that anthocyanin does not affect feed intake, BMI, blood glucose level, cholesterol, and expression of PPAR-? and adiponectin of zebrafish with diet-induced obesity.
Isolation, and Identification of Diesel Oil Degrading Bacteria in Water Contamination Site and Preliminary analysis with Potential Bacterial Gordonia terrae Tri Wahyuni, Ainun Ramadhani; Herawati, Endang Yuli; Kurniawan, Andi; Amin, Abd. Aziz
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.508 KB) | DOI: 10.21776/ub.rjls.2019.006.02.7

Abstract

Bioremediation is one of kind method which use of bacteria to degrade of hydrocarbon to be another compound. This study aims to get isolation of indigenous bacteria and potential test with Gordonia terrae on diesel oil bioremediation. The water sample was taken from Tanjung Perak Port of Surabaya, Indonesia where contaminated with diesel oil. Analysis of in-situ water quality parameters were consist of Dissolved oxygen, temperature, pH, salinity. Strain isolate bacteria were isolated by Bushnell-Hass medium. The bacterial density result shows in IN6 shows that bacterial density increased up to 9 × 108 cells/ml at day 2 incubation and decrease up to 1.79 × 108 cells/ml at final incubation, IN8 increased up to 8.6 × 108 cells/ml, then decrease up to 3.57 × 108 cells/ml. While, EX8 increased up to 9.1 × 108 cells/ml, and then decrease up to 3.06 × 108 cells/ml. While, results of degradation diesel oil hydrocarbon show treatment IN6, TPH values showed a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. IN8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. EX8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 24 ppm. In this experiment was found that Bacillus cereus was effectively degrading of diesel oil and can be agent bacterial for bioremediation.
Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L) Merr) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis Fransira, Immaria; Anggreini, Annisa Fitri; Yanuhar, Uun; Maftuch, Maftuch
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.206 KB) | DOI: 10.21776/ub.rjls.2019.006.02.3

Abstract

Pseudomonas fluorescens is a gram-negative pathogenic bacteria which is one of the problems in fish farming that can causes death in fish. The alternative to overcome this bacterial attack is by using of natural material such as dayak onion bulbs (Eleutherine palmifolia (L) Merr), which are known to have secondary metabolites. The purpose of this research was to determine the antibacterial activity against P. flourescens that contained in the dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction and the content of antibacterial compounds from the best dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction. The method used in this research was antibacterial test with MIC (Minimum Inhibitory Concentration) followed by disc test and FTIR (Fourier Transform Infrared Spectroscopy) to analysis secondary metabolite. Fractionation was conducted using Chloroform: Methanol (9:1 v/v). MIC test result showed that 100 ppm was the minimum concentration that can inhibit the growth of P. fluorescens. Disc test reported that fraction 4 was the best fraction, with the dominant compounds that contained in the bulbs was phenolic compound based on FTIR test. So, the fraction of dayak onion bulbs (E. palmifolia (L) Merr) can be used as potential leads to discover new drugs.
Mental Health of Human trafficking; A Systematic Review Suwetty, Awaliyah Muslimah; Astari, Asti Melani; Wihastuti, Titin Andri
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.884 KB) | DOI: 10.21776/ub.rjls.2019.006.02.6

Abstract

Human trafficking is being global crime worldwide. The practice of human trafficking in the form of human exploitation implicated in human rights violations. Survivors of human trafficking will experience health problems both physical and mental problems. The purpose of this systematic review was to identify and summarize the mental health problems of human trafficking victims. Systematic search conducted on March 12, 2019 in data of Proquest, Springer Link, and Scopus by keyword *human health of human trafficking* between the years of 2013-2018. The result of this search found 14 journals that match with inclusion criteria. The inclusion criteria were peer-reviewed journal articles, written in English, all forms of exploitation, the samples are aged >15 years old. The results shown the incidence of human trafficking greatly affected the mental health of victim. Victims experienced anxiety, depression, isolation, disorientation, aggression, suicidal ideation, attention deficit, psychotic disorders, behavioral and post-traumatic stress disorder (PTSD). For further research required in efforts to overcome the mental health impact of human trafficking victims. The focus on preventing efforts was through government campaigns, IOM and law enforcement and also the fulfillment of the rights of human trafficking victims
Correlation between Density of Vibrio Bacteria with Oscillatoria sp. Abundance on Intensive Litopenaeus vannamei Shrimp Ponds Ariadi, Heri; Mahmudi, Mohammad; Fadjar, Mohamad
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.199 KB) | DOI: 10.21776/ub.rjls.2019.006.02.5

Abstract

The abundance of Vibrio bacteria and the presence of an excess Oscillatoria sp plankton type are the main problems that often arise in intensive vanamei (Litopenaeus vannamei) shrimp culture. To examine this problem, the purpose of this study was to determine of model approach interaction between the abundance of Vibrio and Oscillatoria sp with the shrimp growth rate in ponds. This research was conducted with the ex-pose facto design on intensive shrimp culture operations in Bayeman Village, Probolinggo. The results data from field research variable are analyzed using a dynamic modeling system. From the modeling analysis results, showed that the Oscillatoria sp abundance pattern tended to increase over with shrimp culture period as following the pattern of tropical status dynamics by the nutrient increase load of 16.37%/week in ponds ecosystem. Meanwhile, the increase of Vibrio colonies density in ponds continued to increase aggregately by 0.99%/week on nine weeks and 12.5% / week on the last eight weeks of shrimp culture periods. So, it can be concluded that the fluctuations density of vibrio and Oscillatoria sp bacteria in ponds is are bioecological responses from increased nutrient loads and other micromaterials in ponds due to the longer period of shrimp culture.

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