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Articles 190 Documents
UTILISASI INDEKS TROMBOSIT SEBAGAI PREDIKTOR KEPARAHAN PADA KASUS FEBRIS CURIGA DEMAM DENGUE Arjana, Adika Zhulhi; Yuantari, Rahma; Devita, Ninda; Irfan, Rozan Muhammad
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.9725

Abstract

ABSTRAKIndeks trombosit memiliki karakteristik yang mengarah pada kondisi cairan dan trombosit tubuh. Penelitian mengenai hubungan indeks trombosit pada penentuan derajat keparahan demam dengue membuka peluang menjadikan indeks trombosit sebagai factor prognostik. Tujuan penelitian ini untuk mngetahui hubungan indeks trombosit sebagai prediktor keparahan pada kasus febris curiga demam dengue. Penelitian ini bersifat non eksperimental analitik. Data diambil dengan metode cohort prospektif. Subjek penelitian adalah pasien febris curiga dengue di RS X, Y, dan Z Yogyakarta dan sekitarnya. Subjek kemudian diambil darah untuk diperiksa indeks trombosit pada saat masuk dan hari ketiga perawatan. Penentuan derajat keparahan dengue dinilai pada hari ketiga perawatan. Hubungan antara hasil indeks trombosit dengan diagnosis dianalisis dengan ANOVA sedangkan korelasi antara indeks trombosit saat admisi dan hari ketiga dengan uji korelasi dengan bantuan software Medcalc. Sebanyak 56 subyek masuk dalam penelitian). Analisis ANOVA menunjukkan adanya perbedaan yang signifikan untuk mean platelet volume (MPV) dan platelet distribution width (PDW) antar derajat infeksi dengue (p=0.01 dan p=0.017). Analisis ANOVA untuk plateocrit menunjukkan tidak ada perbedaan yang signifikan (p=0.055). Uji korelasi hasil antar indeks trombosit menunjukkan hubungan yang signifikan. Adanya perbedaan MPV dan PDW pada berbagai derajat infeksi dengue sehingga dapat digunakan untuk memprediksi keparahan infeksi dengue.Kata Kunci: Dengue, Indeks Trombosit, Resusitasi CairanABSTRACTPlatelet index has characteristics that lead to the condition of body fluids and platelets. Study on relationship of platelet index in the determining severity of dengue fever opens the opportunity to make the platelet index as a prognostic factor. The aim of this study was to know the correlation of platelet index with predictors of severity of febrile suspected dengue fever. This study was non-experimental and cohort prospective study. The subjects were febrile patients suspicious of dengue in X, Y, and Z Hospital in Yogyakarta and surrounding areas. Subjects were collected blood sample to be examined for platelet index at admission and third day after. Severity of dengue was assessed on third day after admission. Relationship between platelet index with severity was analyzed by ANOVA while correlation between platelet index was analyzed by correlation test with Medcalc software. A total of 56 subjects were included in the study. ANOVA analysis showed a significant difference in mean platelet volume (MPV) and platelet distribution width (PDW) between the degrees of dengue infection (p= 0.01 and p= 0.017). ANOVA analysis for plateocrit showed no significant difference (p = 0.055). Correlation test results between platelet indices show a significant relationship. The results of this study indicate differences in MPV and PDW in various degrees of dengue infection so that it can be used to predict severity of dengue fever.Keywords: dengue, platelet index, fluid resuscitation
EFEKTIVITAS EKSTRAK AKAR PANDAN WANGI (PANDANUS AMARYLLIFOLUS ROXB.) SEBAGAI ANTIMALARIA TERHADAP JUMLAH MONOSIT DALAM DARAH MENCIT (MUS MUSCULUS) YANG DIINFEKSI PLASMODIUM BERGHEI Dila, Gata; Kahtan, Muhammad Ibnu; Widiyantoro, Ari
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.9237

Abstract

ABSTRAKMalaria merupakan salah satu penyakit infeksi yang memberikan morbiditas yang cukup tinggi di dunia dan merupakan infeksi yang ketiga teratas dalam jumlah kematian. Di Indonesia, pemberantasan penyakit malaria kurang maksimal karena adanya resistensi parasit terhadap obat antimalaria dan gangguan sistem imun. Monosit merupakan sel efektor imun yang penting dalam perlindungan terhadap patogenesis malaria yang bersifat sitostatik dan sitotoksik. Tanaman pandan wangi (Pandanus amaryllifolius Roxb.) memiliki senyawa yang dapat bekerja sebagai antimalaria dan imunomodulator. Tujuan penelitian ini yaitu mengetahui efektivitas ekstrak metanol akar pandan wangi sebagai antimalaria terhadap jumlah parasitemia dan monosit dalam darah mencit (Mus musculus) yang diinfeksi Plasmodium berghei. Penelitian yang dilakukan merupakan penelitian eksperimen murni (true experiment design) in vivo dengan desain rancangan acak lengkap (completely randomized design). Uji efektivitas ekstrak metanol akar pandan wangi sebagai antimalaria dilakukan dengan konsentrasi 6,5%, 13% dan 26%. Kontrol positif diberi 3,74 mg/mL Dihydroartemisinin-Piperaquin (DHP), kontrol negatif diberikan akuades dan kontrol normal tidak diintervensi Hasil penelitian menunjukkan konsentrasi 6,5%, 13% dan 26% memiliki aktivitas antimalaria dengan menurunkan persentase parasitemia dan meningkatkan jumlah monosit. Kesimpulan dalam penelitian ini ekstrak akar pandan wangi memiliki aktivitas malaria dengan konsentrasi yang paling baik yaitu 26% untuk menurunkan jumlah parasitemia dan meningkatkan monosit.Kata Kunci: Pandanus amaryllifolius Roxb. Antimalaria, Monosit, Plasmodium bergheiABSTRACTMalaria is one of the most common infectious diseases in the world and is the third highest infection in mortality. In Indonesia, eradication of malaria is not maximal because of parasitic resistance to antimalarial drugs and immune system disorders. Monocytes are important effector immune cells in the protection of the cytostatic and cytotoxic pathogenesis of malaria. Pandanus amaryllifolius Roxb. root plant have compounds that can work as antimalarials and immunomodulators. This research aim to determine the effectiveness of the methanol extract of Pandanus amoryllifolius Roxb. as antimalarial to parasitemia and monocytes count of blood mice (Mus musculus) with Plasmodium berghei infection. This study is a true experiment design in vivo with complete randomized design. Methanol extract of Pandanus amaryllifolius Roxb. with concentration 6.5%, 13% and 26% used as antimalarial. Dihydroartemisinin-Piperaquine (DHP) 3.74 mg/mL used as positive control, aquades used as negative control groups and the control group was not intervened. The result of this research show effectiveness as antimalarial in methanol extract of Pandanus amaryllifolius Roxb. with concentration 6,5%, 13% and 26% have antimalarial activity by decreasing percentage of parasitemia and increasing monocyte count. The conclusion of this research is the high antimalarial activity in methanol extract of Pandanus amaryllifolius Roxb. in 26% concreation which could decreased parasitemia precentage and increased monocytes count.Keywords: Pandanus amaryllifolius Roxb, Antimalarial, Monocyte, Plasmodium berghei
KARAKTERISTIK FAKTOR RISIKO PASIEN CHRONIC KIDNEY DISEASE (CKD) YANG MENJALANI HEMODIALISA DI RS X MADIUN Arianti, Arianti; Rachmawati, Anisa; Marfianti, Erlina
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.9597

Abstract

ABSTRAKPenderita cronic kidney disease (CKD) terus meningkat setiap tahunnya, bahkan di Indonesia lebih dari 200.000 kasus baru gagal ginjal stadium akhir. Faktor risiko seperti hipertensi, diabetes mellitus, penyakit kardiovaskular, glomerular, tubulointerstitial, ginjal polikistik, penggunaan obat/bahan kimia berpengaruh terhadap kejadian CKD. Penelitian ini bertujuan untuk mengetahui karakteristik faktor risiko pasien CKD yang menjalani hemodialisa. Penelitian ini merupakan penelitian non eksperimental dengan desain penelitian cross sectional. Data diperoleh melalui data sekunder periode Januari?Oktober 2016. Hasil dari penelitian ini adalah dari 88 pasien yang memenuhi kriteria inklusi, terdapat 57 pasien (64,8%) laki-laki dan 31 pasien (35,2%)  perempuan, terdapat 1 pasien (1,1%) CKD pada kelompok usia 18-25 tahun, 26 pasien (29,5%) pada kelompok umur 26-45 tahun, 54 pasien (61,4%) pada kelompok umur 46-65 tahun, 7 pasien (8%) pada kelompok usia >65 tahun. Berdasarkan faktor risiko terjadinya CKD didapatkan hasil 81 pasien (92%) menderita hipertensi, 34 pasien (38,6%) dengan DM, 17 pasien (19,3%) dengan penyakit tubulointerstitial, 6 pasien (6,8%) dengan penyakit ginjal polikistik, 4 pasien (4,5 %) dengan penyakit kardiovaskular, tidak ada pasien (0%) dengan penyakit glomerular. Hipertensi dan diabetes mellitus merupakan dua faktor risiko tertinggi pasien CKD yang menjalani hemodialisa di RS X Madiun.Kata Kunci: CKD, Faktor Risiko, HemodialisaABSTRACTPatients with chronic kidney disease (CKD) continues to increase every year, even in Indonesia, more than 200,000 new cases of end-stage renal failure. There are several risk factors such as hypertension, diabetes mellitus, cardiovascular disease, glomerular, tubulointerstitial, kidney polikistik, use of drugs / chemicals affect the occurrence of chronic kidney disease. The study aims to determine the characteristics of the risk factors of CKD patients undergoing hemodialysis. The study is a non-experimental, using cross sectional design. Data obtained through secondary data by looking at the medical record period from January to October, 2016. The results of this study are from 88 patients who met the inclusion criteria there are 57 patients (64.8%) were male and 31 patients (35,2%) were women, there are 1 patient (1,1%) in the age group 18-25 years, 26 patients (29,5%)  in the age group 26-45 years, 54 patients (61,4%)  in the age group 46-65 years, 7 patients (8%)  in the age group> 65 years. Based on the risk factors of CKD showed 81 patients (92%) had hypertension, 34 patients (38.6%) with DM, 17 patients (19.3%) with tubulointerstitial disease, 6 patients (6,8%) with polycystic kidney disease, 4 patients (4,5%) with cardiovascular disease, no patient (0%) with glomerular disease. Hypertension and diabetes mellitus are the two highest risk factors for CKD patients undergoing hemodialysis in Madiun X Hospital.Keywords: CKD, Risk Factors, Hemodialysis
POLA BAKTERI DAN ANTIBIOGRAM PENYEBAB ULKUS DIABETIKUM DI RSUD ARIFIN ACHMAD PROVINSI RIAU PERIODE 2015 – 2018 Anggraini, Dewi; Yovi, Indra; Yefri, Riza; Christianto, Erwin; Syahputri, Eka Zulya
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.9316

Abstract

ABSTRAKUlkus diabetikum adalah salah satu komplikasi dari diabetes mellitus. Ulkus ini terjadi akibat kerusakan  sistem saraf dan pembuluh darah akibat dari hiperglikemia yang tidak terkontrol. Kurangnya nutrisi dari pembuluh darah serta penurunan sensasi akibat kerusakan jaringan saraf memudahkan terjadinya ulserasi infeksi. Pola bakteri ulkus diabetikum dan resistensi antibiotik perlu diketahui untuk meningkatkan kesembuhan bagi pasien. Penelitian ini dilakukan di RS X Riau periode tahun 2015-2018. Data penelitian didapatkan dari Laboratorium Mikrobiologi RS X Riau. Hasil penelitian menunjukkan bahwa Klebsiella pneumonia adalah bakteri yang paling banyak menginfeksi penderita ulkus diabetikum di Rumah Sakit ini yaitu sebesar 17,9%. Bakteri lain yang menjadi penyebab adalah Escherichia coli (16,5%), Staphylococcus aureus (16,5%), Acinetobacter baumanii (14,7%) dan Pseudomonas aeruginosa (8,7%). Uji sensitivitas antibiotik menunjukkan bahwa Klebsiella pneumonia dan Escherichia coli paling sensitif terhadap antibiotik ertapenem, meropenem, amikacin dan tigecycline. Staphylococcus aureus ditemukan sangat resisten terhadap cefoxitine. Acinetobacter baumanii sensitif terhadap amikacin dan tigecycline, sedangkan Pseudomonas aeruginosa 100% resisten terhadap ampicillin, sefazolin, seftriakson, tygecycline, dan kotrimoksazol.Kata Kunci: Ulkus Diabetikum, Pola Bakteri, Pola Resistensi, Sensitifitas, Antibiotik  ABSTRACTDiabetic ulcer is a condition of diabetes?s complication. These ulcers occur due to damage of nerve tissues and blood vessels caused by uncontrolled hyperglycemia. Lack of nutrition from blood vessels and decreased sensation due to nerve tissue damage facilitates ulceration of the infection. It is important to know how the pattern of bacteria and antibiotic?s resistance to improve disease?s prognosis. This research was conducted at X Hospital Riau in the 2015-2018 periods. The research data was obtained from the Microbiology Laboratory of X Hospital Riau. The results showed that Klebsiella pneumonia was the most infecting bacterium with diabetic ulcer patients at this hospital, which was 17.9%. Other bacteria that cause are Escherichia coli (16.5%), Staphylococcus aureus (16.5%), Acinetobacter baumanii (14.7%) and Pseudomonas aeruginosa (8.7%). Antibiotic sensitivity tests show that Klebsiella pneumonia and Escherichia coli are most sensitive to ertapenem, meropenem, amikacin and tigecycline antibiotics. Staphylococcus aureus was found to be very resistant to cefoxitine. Acinetobacter baumanii sensitive to amikacin and tigecycline and Pseudomonas aeruginosa 100% resistant to ampicillin, cefazolin, ceftriaxone, tygecycline, and cotrimoxazole.Keywords: Diabetic Ulcer, Bacterial Pattern, Resistance Pattern, Sensitivity, Antibiotics
PENGARUH WAKTU SIMPAN TERHADAP PERUBAHAN PH, GLUKOSA, LDH, KALSIUM, MVP SEBAGAI INDIKATOR KUALITAS KOMPONEN DARAH THROMBOCYTE CONCENTRATE Mentari, Diani; Pebrina, Relita; Nurpratami, Diah
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.8981

Abstract

ABSTRAK                Thrombocyte Concentrate (TC) merupakan komponen darah yang ditranfusikan untuk pasien yang mengalami pendarahan, kelainan fungsi trombosit dan trombositopenia. Pada beberapa literatur menyebutkan bahwa sediaan TC secara in vitro dapat disimpan selama 5-7 hari. Quality Control sebelum TC ditransfusikan yaitu melalui pengamatan secara visual ada tidaknya swirling dan melihat tingkat kejernihan TC. Namun pengamatan bersifat subjektif sehingga belum terstandar. Penelitian ini bertujuan untuk melihat pengaruh waktu penyimpanan TC terhadap kualitasnya. Kualitas TC diukur melalui beberapa cara yaitu pengukuran pH, kadar glukosa, Laktat Dehidrogenase (LDH), kalsium serta profil darah yang meliputi jumlah trombosit dan Mean Platelet Volume (MPV). Analisis kadar glukosa, LDH, dan kalsium dilakukan secara kolorimetri menggunakan spektrofotomter sedangkan profil darah diukur menggunakan hematology analyzer. Hasil menunjukkan bahwa TC yang disimpan pada hari ke-9 terjadi penurunan pH sebesar 7,38%, glukosa 20,10%, LDH 42,89%dan kalsium 62,54%. Jumlah trombosit mengalami penurunan sebesar 24,41% dan MVP mengalami kenaikan 18,84%. Kesimpulan pada sampel TC yang masih terdapat swirling, namun terjadi penurunan kualitas TC yang ditandai dengan penurunan jumlah trombosit serta kenaikan nilai MVP. Selain itu semakin lama waktu penyimpanan akan menurunkan kadar pH, kadar glukosa, LDH, dan kalsium. Kata Kunci: Masa Simpan Trombosit, pH, Glukosa, Kalsium, MPV. ABSTRACT                Thrombocyte Concentrate (TC) is the blood component tranfused for the patients with bleeding, abnormalities of platelet function and thrombocytopenia. In some literatures, it is mentioned that the TC preparation in vitro can be stored within 5-7 days.  Quality Control before TC is transfused through a visual observation on the presence or absence of swirling and the clarity level of TC. However, this observation is subjective and makes it unstandardized. This research aims to see the effects of the shelf life of thrombocytes on its quality. The TC quality was measured through a number of ways including: pH measurement, glucose level, Lactate Dehydrogenase (LDH), calcium and profiles of blood including number of thrombocytes, and Mean Platelet Volume (MPV). The analysis on the glucose level, LDH, and calcium was conducted by colorimetrical manner using the spectrophotometer and blood profile was measured using hematology analyzer. The result showed that TC stored in day 9 experienced the decrease of pH at 7.38%, glucose at 20.10%, LDH at 42,89% and calcium at 62.54%. The number of thrombocytes experienced a decrease of 24.41% and MVP experienced an increase of 18.84%. In conclusion, the sample of TC that had swirling experienced a decrease in the TC quality as characterized with the decrease of number of thrombocytes and the increase of MVP value. In addition, the longer storage could decrease the level of pH, level of glucose, LDH, and calcium. Keywords: Platelet Storage, pH, Glucose, Calcium, MPV.
PATTERNS OF TRANSCRANIAL DOPPLER FLOW VELOCITIES AND CAROTID INTIMA MEDIA THICKNESS (CIMT) IN JAVANESE ISCHEMIC STROKE Danuaji, Rivan; Subandi, Subandi; Apriyanto, Arif; Dewi, Endang Kusuma; Suroto, Suroto; Mirawati, Diah Kurnia; Budianto, Pepi; Hambarsari, Yetty; Hamidi, Baarid Lukman; Prabaningtyas, Hanindya Riani; Sulistyani, Sulistyani
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.9921

Abstract

ABSTRACTIntracranial atherosclerosis is the most common cause of stroke in Asia including Indonesia. Transcranial Doppler (TCD) is a noninvasive tool which able to detect intracranial atherosclerosis. The purpose of this study was to determine the pattern of TCD waves in Javanese ischemic stroke patients and Carotid Intima Media Thickness (CIMT), and its correlation to demographic data. This is a cross-sectional study was conducted at Hospital X Surakarta. All Javanese ischemic stroke treated in stroke unit were examine for flow velocity using TCD and CIMT using ultrasound. Chi square was used to analyzes the relationship between age, gender, stroke type and the thickness of CIMT.  A total of 43 ischemic stroke patients were examined for TCD, consisting of 30 (69.77%) men and 13 (30,23%) women. The results showed that 31 (72.09%) had intracranial abnormalities in the form of stenosis or occlusion on MCA (23.26%), ACA (4.65%), PCA (4.65%), vertebral arteries (6.98 %) and other locations (32.56%). Thickening of CIMT was obtained as much as 22 (51,16%) of the patients. Age was a factor associated with CIMT thickening with (P: 0.041), while gender and type of stroke did not (P>0,05)). To sum up Intracranial flow velocity abnormalities often occur in Javanese ischemic stroke patients, while CIMT thickening is not associated with ischemic stroke pathologyKeywords: TCD, Stroke Ischemic, Flow Velocity, CIMT, JavaneseABSTRAKAterosklerosis intrakranial adalah penyebab paling umum dari stroke di Asia termasuk Indonesia. Transcranial Doppler (TCD) adalah alat noninvasif yang mampu mendeteksi aterosklerosis intrakranial. Tujuan dari penelitian ini untuk mengetahui pola gelombang TCD pada pasien stroke iskemik suku Jawa dan Carotid Intima Media Thickness (CIMT), dan korelasinya dengan data demografi. Penelitian cross-sectional ini dilakukan di Rumah Sakit X Surakarta. Semua stroke iskemik suku Jawa yang dirawat di unit stroke diperiksa untuk kecepatan aliran menggunakan TCD dan CIMT menggunakan ultrasound. Chi square digunakan untuk menganalisis hubungan antara usia, jenis kelamin, jenis stroke dan ketebalan CIMT. Sebanyak 43 pasien stroke iskemik diperiksa untuk TCD, terdiri dari 30 (69,77%) pria dan 13 (30,23%) wanita. Hasil penelitian menunjukkan bahwa 31 (72,09%) memiliki kelainan intrakranial dalam bentuk stenosis atau oklusi pada MCA (23,26%), ACA (4,65%), PCA (4,65%), arteri vertebral (6,98%) dan lokasi lain (32,56%). Penebalan CIMT diperoleh sebanyak 22 (51,16%) dari pasien. Usia adalah faktor yang terkait dengan penebalan CIMT dengan (p= 0,041), sedangkan jenis kelamin dan jenis stroke tidak (p> 0,05), sehingga ditarik kesimpulan bahwa kelainan kecepatan aliran intrakranial sering terjadi pada pasien stroke iskemik Jawa, sedangkan penebalan CIMT tidak berhubungan dengan patologi stroke iskemik.Kata Kunci: TCD, Stroke Iskemik, Flow Velocity, CIMT, Suku Jawa
PERBEDAAN JUMLAH TELUR CACING GEOHELMINTH ANTARA SAYURAN DI PASAR TRADISIONAL DAN PASAR MODERN DI SURAKARTA Bestari, Rochmadina Suci; Safitri, Aulia Nanda; Purnama, Ayu Arista
Biomedika Vol 12, No 1 (2020): Biomedika Februari 2020
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v12i1.8688

Abstract

ABSTRAKInfeksi cacingan yang disebabkan oleh geohelminth masih terbilang tinggi di Indonesia, yaitu antara 2,5-62 % pada semua umur. Geohelminth  yang sering didapati di Indonesia terdiri dari tiga macam, yaitu Ascaris lumbricoides, hookworm dan Trichuris trichiura. Penularan infeksi geohelminth bisa melalui makanan yang dimakan manusia tanpa dimasak dahulu, larva menembus kulit, dan inhalasi. Jenis sayuran dimakan tanpa dimasak dahulu adalah selada, kemangi dan kubis. Sayuran ini bisa ditemukan di pasar tradisional dan pasar modern. Oleh karena itu, peneliti ingin meneliti perbedaan jumlah telur cacing yang terdapat pada sayuran di pasar tradisional dan pasar modern di Surakarta. Jenis penelitian ini adalah deskriptif, dengan rancangan penelitian cross-sectional. Jumlah sampel sebanyak 18 sampel. Pengambilan sampel penelitian dilaksanakan di 3 pasar tradisional dan 3 pasar modern di Surakarta. Pemeriksaan metode sentrifugasi dan pengamatan dengan mikroskop di Sub Laboratorium Biokimia FK UMS, Surakarta. Hasil penelitian didapatkan rerata jumlah telur geohelminth pada sampel sayuran kemangi yang dijual di pasar tradisional sebanyak 2,46% dan di pasar modern sebanyak 1,25%, sedangkan tidak terdapat kontaminasi telur geohelminth dari sampel sayuran kubis dan selada. Jenis telur yang terdapat pada sayuran kemangi adalah telur hookworm sebanyak 100%. Bisa disimpulkan bahwa rerata jumlah telur geohelminth pada sampel kemangi dari pasar modern lebih sedikit daripada pasar tradisional. Oleh karena itu, sebaiknya pencucian sayuran dilakukan secara seksama untuk menghilangkan semua telur geohelminth yang terdapat pada sayuran sebelum dikonsumsi manusia.Kata kunci: Geohelminth, Sayuran, Pasar Tradisonal, Pasar ModernABSTRACTGeohelminth infection incidence is still high in Indonesia, 2,5-62% in all ages. Geohelminth causing infection in Indonesia are Ascaris lumbricoides, hookworm and Trichuris trichiura. The transmission of geohelminths are by fecal oral (raw vegetables), larvae infestation and inhalation. Non-cooked food is raw vegetables, for example: lettuce, basil and cabbage. The vegetables found at traditional and modern market. The aim of this research is to know the differences in the number of geohelminths egg between raw vegetables sold at traditional market and modern market. It is descriptive, with cross sectional design. The sample size are 18 vegetables, 3 kinds of vegetables taken from 3 tradisional market, and another 3 kinds are taken from 3 modern market. This research used sentrifuge method and the preparat were investigated by microscope to see geohelminths eggs. The result of this research are the mean of geohelminth egg on basil was 2,46% from traditional market and 1,25% from modern market. The kind of the egg was hookworm. There was no contamination of geohelminth egg on lettuce and cabbage of those markets. It can be concluded that the mean of the egg in traditional market was higher than modern markets. For that reason, there should be good handle of basil before it consumed by human.Key words: Geohelminth, Vegetables, Traditional Market, Modern Market
GEJALA KLINIS SEBAGAI PREDIKTOR PADA KARSINOMA SEL BASAL Pramuningtyas, Ratih; Mawardi, Prasetyadi
Biomedika Vol 4, No 1 (2012): Biomedika Februari 2012
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Basal Cell Carcinoma (BCC) incidence is 75% of all skin cancers. BCC rarely causing mortality, but morbidity is high. Clinical diagnostic is the early detection of BCC suspicion, therefore, we required specific criteria that help to establish the clinical diagnosis. The objective of this study was to know the clinical sign as a predictor of basal cell carcinoma. Ten patients with skin tumors who came to dermatovenereological department of Dr. Moewardi hospital were included in this study. Clinical diagnosis using clinical criteria from keratinocyte carcinoma in A Cancer Journal For Clinicians in 2011 (Teleangiectasion, raise border, erythematous with bleeding, pigmentation, translucent appearance, and scarlike appearance) was used as reference of clinical diagnostic criteria. Histopathological examination was performed as a gold standard. All 10 subjects had histopathological finding consistent to BCC. The clinical examination like pigmentation were found in all subjects while teleangiectasia, raise border, erythema with bleeding were found on 9 subject. Translucent appearance was found on 6 subjects. While scarlike appearance was absent in all subjects. The criteria from keratinocyte carcinoma in A Cancer Journal For Clinicians in 2011 can be used to diagnosed BCC clinicallyKeywords : Basal cell carcinoma, Clinical Diagnostic Criteria
Korelasi Jumlah Netrofil, Limfosit, dan Monosit dengan Kadar Albumin Urin pada Pasien DM Tipe 2 dengan Mikroalbuminuria Purwanto, Edy
Biomedika Vol 1, No 1 (2009): Biomedika Februari 2009
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

The most common complication of diabetes is diabetic nephropathy which is a progressive disease and in the terminal stages, needs a very large cost of care. Nephropathy diabetic can be prevented if it is detected earlier. Microalbuminuria is a test to detect nepropathy diabetic early nowadays, but it is still considered expensive by the majority of the community and has not been widely done in laboratories. What ever it needs other alternatives, such as neutrophils, lymphocyte, and monocyte counts. The purpose of this study is to analyse the correlations between neutrophils, lymphocyte, monocyte counts and urine albumin levels in type2 diabetes with microalbuminuria. The research design is analytic observational with cross sectional approach. Twenty two patients met the inclusion criteria. Their urine albumin levels tested by Nycocard® U-ALBUMIN reagent. Neutrophils, lymphocyte, monocyte count was examined by hematology analyzer CELL-Dyn 3700. The correlations between neutrophils, lymphocyte, monocyte counts and urine albumin levels analyzed using coefficient correlation Spearmans. No correlation between neutrophil countsand urine albumin levels (r= -0,250;p=0,263). No correlation between monocyte counts and urine albumin levels (r= -0,191;p=0,395). No correlation between lymphocyte counts and urine albumin levels (r=0,130;p=0,565) in type 2 diabetes with microalbuminuria. No correlation between neutrophils, lymphocyte, monocyte counts and urine albumin level in type 2 diabetes patients with microalbuminuriaKeywords: type 2 diabetes, neutrophils, lymphocytes, monocytes, microalbuminuria.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA , MSi., Dr. Muhtadi; Ambarwati, Ria; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

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