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Bioeksperimen: Jurnal Penelitian Biologi
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Articles 124 Documents
STUDI PENERAPAN BANK SAMPAH DALAM UPAYA PENGELOLAAN LINGKUNGAN HIDUP DI KOTA YOGYAKARTA Haryanti, Sri; Gravitiani, Evi; Wijaya, Mahendra
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10434

Abstract

Waste Bank program is an alternative in waste management by 3R (Reduce, Reuse, Recycle) approach. Yogyakarta is one of the cities that has implemented that program. This study was aimed to analyze the composition of the deposited waste to the banks and examine the implementation of waste banks in Yogyakarta city in environment management efforts. The study was conducted in October-December 2016, and  is a quantitative study that was conducted by measurement, interview and observation methods in five waste banks in Yogyakarta City. Data analysis was performed by means of quantitative descriptive in the form of tables and graphs. The results show that total weight of the deposited waste in a month is 3,062.1 kg, in average. The composition of the waste consists of: plastic 23.36%, paper 65.06%, glass 5% and metal 6.56%. The Waste Bank program in Yogyakarta City is able to assist the efforts of environment management, with a potentiton of 97%  waste reduction. After the existence of waste banks, in corresponds with their environment condition, 65 respondents (65%) stated it is clean, and 35 (35%) stated very clean. Respondents who stated that their houses are very clean and clean are 34% and 66%, respectively.
ISOLASI DAN KARAKTERISASI BAKTERI INDIGEN PENGURAI LEMAK PADA LIMBAH CAIR BATIK TULUNGAGUNG Fidiastuti, Hasminar Rachman; Lathifah, Anis Samroatul; Amin, Mohamad; Utomo, Yudhi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10430

Abstract

The native bacteria have a wide life habitat and have an important role as agents in the bioremediation process of waste. Batik as one of the products of Indonesian character, has become a contributor of liquid waste that can reduce water quality. Besides being rich in dyes, batik waste is also rich in fat content. The fat content in batik waste comes from the processing (coating the fabric with a layer of wax) and washing is done repeatedly. Fat has properties that are not soluble in water and is an organic material, which if high concentrations can reduce water quality. This study aims to isolate, identify, and measure the ability of indigenous bacteria to reduce fat content in liquid waste in vitro. The method used is experimental research. Isolation and culture using Busnall Hass media enriched with 1% olive oil, then proceed with a series of biochemical tests for the purpose of characterizing indigenous bacterial isolates. The results showed that Bacillus subtilis and Pseudomonas fluorescens have the potential to reduce the fat content of batik liquid waste.
MEDIA ALTERNATIF CAMPURAN DAUN PISANG KERING DAN KULIT JAGUNG UNTUK MENINGKATKAN PRODUKTIVITAS JAMUR MERANG (VOLVARIELLA VOLVACEA (BULL) SINGER.) DALAM KERANJANG Suparti, Suparti; Safitri, Wardani Ana
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10435

Abstract

The growth of mushroom requires nutrients such as cellulose, hemicellulose and lignin which can be obtained from rice straw. However, the availability of rice straw at certain times is difficult to obtain. Dried banana leaves and corn husk have the potential to be used as alternative growing media because they contain cellulose, hemicellulose and lignin for the growth of mushrooms. This research was conducted to determine the productivity of straw mushrooms on a mixture of dried banana leaf media and corn husk planted in a basked. The method used RAL (Completely Randomized Design) which consisted of one factor, namely a mixture of 500gr, 375gr, 250gr, 125gr, and 0gr corn shells with Ogr dried banana leaves, 125gr, 250gr, 375gr, and 500gr. Data were tested by one-way Anova analysis. The result showed that there was no effect of the mixture of dried banana leaves and corn husk on the productivity of straw mushrroms. Mushroom can grow on all treatments.
BIOMASSA DI ATAS TANAH DAN PENGHITUNGAN SIMPANAN KARBON HUTAN KALIBIRU KABUPATEN KULON PROGO Arsalan, Andy; Gravitiani, Evi; Irianto, Heru
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10426

Abstract

Above Ground Biomass (AGB) forest ecosystem is one of the fundamental parameters to describe each of its functions. Carbon in the forest ecosystem accumulates through absorption of CO2 in the atmosphere and is assimilated into biomass. The study of forest vegetation biomass is important for determining carbon storage in dominant tree components and calculating carbon cycles at regional and global levels. Kalibiru Forest carbon storage information is not yet available, so it is necessary to calculate carbon potential. This study aims to calculate aboveground biomass and estimate carbon stocks in the Kalibiru Forest. The study was conducted in Kalibiru Hamlet, Hargowilis Village, Kokap District, Kulon Progo Regency. The research conducted the indirect method (non-destructed) to calculate biomass and carbon by using the allometric equation. Base on the calculation, Kalibiru Forest has 5.086,52 tons of biomass or 175,40 tons per hectare in average. The potential for carbon storage in the Kalibiru Forest is 2.543,26 tons of carbon or 87,7 tons per hectare in average. This figure means that preserving 1 hectare of Kalibiru Forest can avoid the impact of atmospheric damage due to the greenhouse gas effect as many as 87,70 tons per year. Results of this study provide an insight for the surrounding community about the importance of the existence of the Kalibiru Forest as an absorber of CO2 gas in the atmosphere. As another benefit, it provides motivation for the surrounding community to continue in preserving the forest.
KUALITAS BIOPLASTIK DARI UMBI SINGKONG KARET DENGAN PENAMBAHAN KOMBINASI PLASTICIZER GLISEROL DENGAN SORBITOL DAN KITOSAN Asngad, Aminah; Marudin, Ervian Jan; Cahyo, Devi Setyaning
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10431

Abstract

High plastic demand has the potential to cause problems because plastic is a synthetic polymer made from petroleum that is difficult to decipher. Therefore, saving the current development of biodegradable plastics that can be decomposed by microorganisms naturally. Cassava rubber tubers are rarely used because they contain HCN toxin but have a carbohydrate content of 98.4674%, so it has the potential as a bio-plastic material. The purpose of this study was to determine the Bioplastic Quality of Cassava Rubber with the addition of a combination of Plasticizer Glycerol with Sorbitol and Chitosan. The study was conducted in the lab. Biology FKIP UMS, research methods used are experimental research methods. The study design used was completely randomized design with 2 treatment factors, namely: treatment factor 1 volume of glycerol (G), G 1 = 3ml; G 2 = 5ml; G 3 = 7ml. Treatment factors are 2 types of Plasticizer (P), P1 = Sorbitol and; P2 = Chitosan. The analysis used is descriptive qualitative used to test tensile resistance, elongation at break. Based on the results of the study showed the strongest spacer resistance, G1P1 which is 17.20N / mm², and the highest breaking length, G3P1 which is 24.00%. with sorbitol and chitosan.
VARIASI PERTUMBUHAN DAN PEMBUAHAN KLON JATI (TECTONA GRANDIS L.F.) UMUR 11 TAHUN Baskorowati, Liliana; Adinugraha, Hamdan Adma; Mashudi, Mashudi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10427

Abstract

The development of teak clones (Tectona grandis L.f.) is currently being done by industry and farmers to increase productivity. The use of superior teak clones will determine the success of planting; therefore, the superior clones need to be developed. Knowledge of variations in growth and fruiting in the seed orchard of teak clones is important to determine the reproductive capacity of a plant. Therefore, study on variations in the growth and fruiting of teak clones was undertaken. The study was conducted at 11 years old of teak clones, located in Alas Ketu Wonogiri, Central Java. The teak plants measured were planted with a spacing of 2 x 6 m, using a randomized block design, with 100 clones; 3 ramet per clone and consists of 5 blocks as replications. The clones originated from Gunung Kidul, Madiun, Cepu, Wonogiri, Randublatung, Rembang, Muna, Matakidi, Kendari, Buton, and Thailand. All individual tress in the plots were examine for collecting data. Parameters were measured including height of trees, diameter breast height, flower and fruit production. The results showed that height and diameter growth varied significantly between clones, as well as between replications; while flower and fruit production did not show significant differences between clones and between replications. When the clones grouped in height class and diameter class, it showed that the production of flowers and fruits of teak clones were differs significantly. Moreover, the reproductive success of this teak clone seed orchard was very low at 2.15%, suggested due to very little rainfall during the flowering season which causes many flowers to fall out.
PERBANDINGAN KEMAMPUAN MIGRASI ADIPOSE-DERIVE STEM CELLS ASAL MANUSIA PADA BERBAGAI MEDIUM PERTUMBUHAN Rosadi, Imam; Karina, Karina; Rosliana, Iis; Widyastuti, Tias; Wahyuningsih, Komang A; Sobariah, Siti; Afini, Irsyah
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10432

Abstract

Cell migration is natural process that plays an important role in development of organisms. Stem cells are an important in the cells migration process due to repairing tissues or organs. Stem cells ability studies towards cell migration process have been developed and studied. In this study, adipose-derived stem cells (ADSCs) were used as cell models of stem cell migration which cultured on medium containing 10% fetal bovine serum (FBS), a combination of 1 0% FBS and L-ascorbic acid (LAA), 10% human platelet-rich plasma (PRP) and 0.1% FBS as a control. The technique that used to analyse cell migration process is scratch assay method. The results of this study indicate that ADSCs were able to migrate on medium containing 10% FBS, 10% FBS-LAA and PRP. On 24 h of observation, the scratched area of ADSCs were 0.70x (control), 0.13x (10% FBS), 0.04x (10% FBS-LAA), and 0.21x (10% PRP) compared to 0 h (1.00x). The width of scratches area in the control group at 24 h was significantly higher than the other treatment groups. This is suggested due to the lack of growth factor contained in the medium as a nutrition for the cells. Therefore, it can be concluded that ADSCs are able to migrate on various medium supplemented by FBS or human PRP.
ANALISIS KEDALAMAN LAUT PURBA BATU GAMPING GORONTALO BERDASARKAN KANDUNGAN FOSIL FORAMINIFERA BENTONIK Permana, Aang Panji; Eraku, Sunarty Suly
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10428

Abstract

The research location is in the form of highland morphology in the Gorontalo City. Research material in the form of limestone with research methods in the form of field surveys and micropaleontological analysis. The purpose of this research is to find out the bentonic foraminifera fossil species and to know paleobathymetry. The results of the analysis there are four fossil species namely Biloculinella depressa, Cornuspira foliacea, Pyrgo laevis and Rhabdammina discreta. Based on the content of bentonic foraminifera fossils, it can be determined that paleobathymetry is Middle Neritic - Outer Neritic (20-200 meters).
TREE COMMUNITY COMPOSITION AND STRUCTURE OF CACAO (THEOBROMA CACAO L.) BASED AGROFORESTRY IN WEST SUMATERA, INDONESIA Santhyami, Santhyami; Basukriadi, Adi; Abdulhadi, Rochadi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10433

Abstract

The aim of this study is to obtain basic descriptive account of structure and floristic composition of cacao based agroforestry in conserving tree species diversity in West Sumatera. An analysis of the composition and structure of three types of cacao based agroforestry system (AFS) [cacao - rubber (CR), cacao ? coconut (CC) and cacao multistrata (CM)] was conducted in April to June 2017. Data collection was done by using quadrat method of 20 x 20 meter plots with a total of 6 plots on each types of AFS. Herbarium specimens were collected and identified. Among all types of these AFS, CC was the most diverse floristically (H? = 1.02) with the highest total of species (10 species), followed by CM (9 species) and CR (7 species). CM has the highest tree density of 1108 trees per ha and the highest total of basal area (30.53 m2). We also did a comparison of the floristic composition of cacao based AFS with the nearest natural forest from previous study. There was a large decrease in the number of non-cocoa tree species from natural forest to AFS, from 139 to 6-9 species. The only native tree species found both in the forest and AFS is Pterospermum javanicum Jungh. It is concluded that three types of cocoa based AFS in West Sumatera were floristically different with natural forest.
TINGKAT KEANEKARAGAMAN HEWAN TROBLOBIONTS PADA EKOSISTEM GUA DI TAJUR BOGOR JAWA BARAT Marhento, Giry; Alamsyah, Mashudi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10429

Abstract

The purpose of this study was to determine the level of diversity of Troglobionts animals, species composition, species dominance, and uniformity in the Cave of Tajur Bogor, West Java. The variables in this study consisted of survey methods by calculating diversity, dominance, uniformity, and species composition to find out what types of animals are in the ecosystem of the cave. Based on the results of research on animal species found there were 10 species of biota belonging to 3 transects with a total of 88 species of cavernous biota. The sample in this study used the 10x10 M line transect method three times in different places. Determination of line transect measurements in this study is based on differences in the environmental zone at the outermost point or cave line which is the starting point of line transect measurement. The results of statistical calculations are obtained as follows: diversity biota index (H ') in the range of 0.4168-0.9684, the index of species composition (P) ranges between 0.2386-0.3861, the uniformity index (E) range between 0,4304-1, and the dominance of type (C) ranges between 5,2118-5,2118. Based on the calculation of diversity, composition, uniformity, and dominance of the type of biota in the area of Tajur Bogor, West Java, at the low-moderate level. Overall the types of biota found in this study were 3 transects, generally producing different amounts during the study, the total biota species found in the cave consisted of 88 individuals from 14 families and 14 species while the highest composition of biota types in transect III where of the 34 species found throughout the transect, 33 were in the second transect and the lowest was in transect I with only 21 species.

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