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INDONESIA
INDONESIAN JOURNAL OF PHARMACY
ISSN : 23389427     EISSN : 23389486     DOI : -
Core Subject : Health,
Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.
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Articles 8 Documents
Search results for , issue " Vol 13 No 2, 2002" : 8 Documents clear
REDUCTION ABILITY OF CURCUMIN AND DERIVATIVES ON FERRI : A MOLECULAR DYNAMIC STUDY Ari Sudarmanto, Bambang Sulistyo; Margono, Supardjan Amir
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.157 KB) | DOI: 10.14499/indonesianjpharm0iss0pp77-85

Abstract

Molecular dynamics (MD) simulation has been applied to curcumin, 4-methyl curcumin and 4-isopropil curcumin. They have reducting activities on Ferri to Ferro. A computation method used is AM1 (Austin Model 1) semiempiric method. Studying of computation is arranged to theoretical studying of structure and reactivity relationship on these molecules without solvent interaction (in vacuo). The time of simulation is taken from 0 – 10 ps (pico secon) with interval 0,0005 ps on 300oK. The result of experiment that has been proposed from the MD simulation is the structure of conformation trajectory of each molecules. By considering total energy and potential energy changes to time of simulation, along with the change of bonding length, bonding angle, and the atomic charges of -diketon active site to time of simulation. Reactivity of these molecules are analyzed from the geometric shape of the active site indicated that 4-isopropyl curcumin was the most reactive than the other to produce complex with Ferri, while 4-methyl curcumin within the complex was easiest in the reduction of Ferri to Ferro.Key words : molecular dynamic, curcumin, reduction
PRESCRIPTION ANALYSIS: AN INVESTIGATION ON PRESCRIPTION LEGALITY IN THE PHARMACIES OF KOTAMADYA YOGYAKARTA Rahmawati, Fita; Oetari, R.A.
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.185 KB) | DOI: 10.14499/indonesianjpharm0iss0pp86-94

Abstract

A research has been carried out to identify whether prescriptions received in pharmacies at Yogyakarta district fulfilled the legality as the regulation concern. This included an investigation on prescription hand-writing, part of a prescription which has a potential to bring about a medication error due to misinterpretation.The study was conducted by collecting prescription samples, randomly picked-up (=5% and d=3%), from each of 12 pharmacies. Furthermore, other supporting data, such as questioner and interview, were also collected from 29 pharmacies involving 83 respondents (24 pharmacists and 59 technician assistants). Data were then analyzed descriptively.The result showed that prescriptions fulfilled the regulation was approximately 39.8%. The absences of sign or license number of prescriber, the date of prescription were only examples of the cause. Hand-writing of the prescriber was sometime so bad that causing difficulty to interpret especially name of medicine, dose, usage or administration, uncommon abbreviation, hence increasing the risks of medication error to happen.Key word: prescription, legality aspect of prescription, medication error
DUAL EFFECTS OF FLAVONOID QUERCETIN ON RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELLS : INHIBITS HISTAMINE RELEASE AND REDUCES CELL GROWTH Ikawati, Zullies; Wallgard, Elizabeth; Maeyama, Kazutaka
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.619 KB) | DOI: 10.14499/indonesianjpharm0iss0pp55-64

Abstract

The dual effects of the flavonoid quercetin on rat basophilic leukemia cells (RBL-2H3), tumor analog mast cells, was studied. Quercetin is known as an anti-inflammatory drug that inhibits mast cell secretion of histamine. This study aims to investigate the consequences of varying incubation times with quercetin on 2H3 cells to histamine release inhibitory action and the effects of long time incubation to the morphology of the cells. The effect of quercetin on histamine synthesis was also observed. The histamine release from the cells was inhibited by quercetin as expected, but the ability of secretion was rapidly recovered when quercetin was removed before challenging. Incubation up to 6 hours decreased the inhibitory action, but longer than 6 hours increased the inhibitory activity. In long time incubation, the cells exhibited cell damage, decreased cell growth, morphological changes, and detachment from the underlying surface in proportion with the concentration of quercetin. The instant and reversible inhibitory effects of quercetin appear to represent a first phase of actions, while reduced cell growth, elevated cell damage and morphological changes seem to be connected to a second phase. Consequently, quercetin could be considered as a compound that acts dually on RBL-2H3 cells.Key words : quercetin, histamine, RBL-2H3 cells
BIOTRANSFORMATION OF CINCHONA ALKALOID COMPOUNDS BY MOLD Xylaria sp.TO CINCHONA N-OXIDE ALKALOIDS [Studies on Endophytic Microbes of Cinchona spp. plants (5)] Simanjuntak, Partomuan; ., Bustanussalam; Prana, Titik K.; Ohashi, K.; Shibuya, Hirotaka
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.247 KB) | DOI: 10.14499/indonesianjpharm0iss0pp95-100

Abstract

A biotransformation study on alkaloid chinchona compounds (quinine, quinidine, cinchonidine, cinhonine) by endophytic microbe from Cinchona succirubra have been done. The biotransformation for 2 days after preculture yielded cinchona N-oxide compounds (quinine N-oxide, quinidine N-oxide, cinchonidine N-oxide, cinchonine N-oxide) alkaloids and the bioassay of the compounds also showed effectiveness as antimalaria. Purification the biotransfomed compound was conducted by a high performance liquid chromatograph (HPLC) and determination of chemical structure was done based on carbon nuclear magnetic resonance (NMR).Key words : Biotransformation, endophyte microbe, Xylaria sp., Cinchona succirubra Pav., cinchona alkaloid, quinine, quinine N-oxide
EFFECT OF ASPIRIN ON RAT LIVER CLASS- GLUTATHIONE S-TRANSFERASE ACTIVITY Istyastono, Enade Perdana; Martono, Sudibyo; A.M., Supardjan
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp71-76

Abstract

Inflammation is the response of living tissues caused by injury. It involves a complex battery of enzyme activation, mediator release, extravasation of fluid, cell migration, tissue breakdown and repair. Inflammation mediator is synthesized in the body by several steps and catalyzed by several enzymes, such as cyclooxygenase and class- glutathione S-transferases (GSTs) in the cyclooxygenase arachidonic acid cascade. Aspirin, which has been reported as an inhibitor to inflammation mediator synthesis in the cyclooxygenase arachidonic acid cascade, is a non-steroid anti-inflammatory agent. The aim of this research is to study the effect of aspirin on rat liver class- GST activity in vitro. GSTs were isolated from the rat liver cytosolic fraction by centrifugation according to Lundgren. Protein concentration in the cytosol was determined spectrophotometrically using bovine serum albumin as a standard. The GST activity was determined using conjugation reaction rate between glutathione (GSH) and 1,2-dichloro-4-nitrobenzene (DCNB), followed by determining IC50 of aspirin. Then, a study was done to determine the ability of aspirin in inhibition of the rat liver class- GST activity in vitro. It can be concluded that aspirin has no inhibitory effect on rat liver class- GST activity.Key word: Aspirin, anti-inflammatory, glutathione S-transferase.
TOXICITY TEST OF MAKUTADEWA (Phaleria macrocarpa (Scheff.)Boerl.) BARK AGAINST Artemia salina Leach AND THIN LAYER CHROMATOGRAPHY PROFILE OF THE ACTIVE FRACTION Hertiani, Triana; Tanjung Pratiwi, Silvia Utami
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.013 KB) | DOI: 10.14499/indonesianjpharm0iss0pp65-70

Abstract

Makutadewa (Phaleria macrocarpa (Scheff) Boerl.) is one of the herbal medicines used against cancer, however, the scientific basis of the activity still void. Daphne mezereum, the same family (Thymelaeaceae) to makutadewa has been proved to contain cytotoxic substances in its bark. Therefore, the aims of this research were to investigate the toxic effect of makutadewa bark against Artemia salina Leach. as the primary step to identify an anticancer activity and find out substances responsible to this activity. The material powder was extracted using Soxhlet apparatus with chloroform, followed by methanol and finally by distilled water. The toxicity of chloroform, methanolic and aqueous extracts were assayed against A.salina Leach. The chloroform extract (most active) with the LC50 of 29.6  0.14 g/ml was fractionated by vacuum liquid chromatography using wash benzene 100 % (a); wash benzene-ethyl acetate =20:1 (b); 15:1 (c); 10:1 (d); 5:1 (e); 2:1 (f) and chloroform-methanol 1:1 (g) as mobile phases. The active fractions against A.salina were E {combination of (e) and (f)} with LC50 of 106,9 g/ml and F (g) with the LC50 of 131,53 g/ml, hence less toxic than the original extract. The thin layer chromatogram profiles showed that E and F fractions contained terpenoid and alkaloid substances.Key words: toxicity test, (Phaleria macrocarpa (Scheff)Boerl.), bark
TOXICITY OF ETHANOLIC EXTRACT OF FRUIT, SEED AND LEAVES OF MAKUTADEWA (Phaleria macrocarpa (Scheff.) Boerl.) TO Artemia salina Leach AND THIN LAYER CHROMATOGRAM PROFILE OF ACTIVE EXTRACT Purwantini, Indah; Setyowati, Erna Prawita; Hertiani, Triana
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.799 KB) | DOI: 10.14499/indonesianjpharm0iss0pp101-106

Abstract

A research has been done to determine the toxicity of ethanolic extract of fruit, seed and leaves of makutadewa (Phaleria macrocarpa (Scheff) Boerl.) using Brine Shrimp Lethality Test method. Extract were made by maceration of the materials for 24 hours. The bioassay has been done using 48 hour old Artemia saline Leach. The effect of ethanolic extract was identified by determining and analysing the shrimp death percentage and LC50 of each extract using probit analysis.The result showed that these ethanolic extract were toxic to the shrimp. The ethanolic extract of seed was the most active with the LC50 of 1.60 x 102 g/ml, whereas that of the fruit has the LC50 of 30.42 g/ml. The thin layer chromatogram profile of the active extract suggested that the extract contains alkaloids.Key words : toxicity, makutadewa, brine shrimp lethality test
EFFECT OF ASPIRIN ON RAT LIVER CLASS- GLUTATHIONE S-TRANSFERASE ACTIVITY Istyastono, Enade Pradana; Martono, Sudibyo; AM, Supardjan
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 2, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.357 KB) | DOI: 10.14499/indonesianjpharm0iss0pp71-76

Abstract

Inflammation is the response of living tissues caused by injury. It involves a complex battery of enzyme activation, mediator release, extravasation of fluid, cell migration, tissue breakdown and repair. Inflammation mediator is synthesized in the body by several steps and catalyzed by several enzymes, such as cyclooxygenase and class- glutathione S-transferases (GSTs) in the cyclooxygenase arachidonic acid cascade. Aspirin, which has been reported as an inhibitor to inflammation mediator synthesis in the cyclooxygenase arachidonic acid cascade, is a non-steroid anti-inflammatory agent. The aim of this research is to study the effect of aspirin on rat liver class- GST activity in vitro. GSTs were isolated from the rat liver cytosolic fraction by centrifugation according to Lundgren. Protein concentration in the cytosol was determined spectrophotometrically using bovine serum albumin as a standard. The GST activity was determined using conjugation reaction rate between glutathione (GSH) and 1,2-dichloro-4-nitrobenzene (DCNB), followed by determining IC50 of aspirin. Then, a study was done to determine the ability of aspirin in inhibition of the rat liver class- GST activity in vitro. It can be concluded that aspirin has no inhibitory effect on rat liver class- GST activity.Key word: Aspirin, anti-inflammatory, glutathione S-transferase.

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