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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 90 Documents
MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY BACTROCERA OCCIPITALIS (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE Sudiarta, I Putu; Martiningsia, Dwi; Wijaya, I Nyoman
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v06.i01.p03

Abstract

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5?-GGAGCATTAATYGGRGAYG-3? as forward primer and HCO 5?-TAAACTTCAGGGTGACCAAAAATCA-3? as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.
MORPHOLOGY, PHYSIOLOGY AND MOLECULAR CHARACTERISTICS OF OIL PALM (ELAEIS GUINEENSIS JACQ.) ENDOPHYTIC BACILLUS SP. Puspita, Fifi; Hadiwiyono, Hadiwiyono; Poromarto, Susilo Hambeg; Roslim, Dewi Indriyani
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v05.i01.p07

Abstract

Endophytic bacteria are the bacteria that live in plant tissues. In oil palm tissue there are many types of endophytic bacteria and have a role that can be beneficial for the plant, one of them is endophytic Bacillus sp. The aim of these research was to obtain morphology, physiology and molecular characteristics of endophytic Bacillus sp. originating from oil palm tissue. Sampling was done by random simple sampling method. Isolation of bacteria was performed on plant tissues such as roots, midribs, stems and leaves of oil palm plants. The results of morphological characterization such as colony color, colony shape and colony edge show similarity in each isolate but there are differences in the surface morphology of the colony, where there are 6 isolates with convex surface and 6 isolates with flat shape. Physiological test results such as catalase test, oxidase test, starch hydrolysis test, motility test and temperature effect test on bacterial growth showed positive results in each isolate. Molecular characterization using 16S rRNA primers based on BLASTn shows that all isolates tested have similarities with Bacillus sp. Based on the phylogenetic tree it was found that the endophytic bacteria of Ba-B2 isolates were associated with Bacillus flexus with 100% consistency index grouped at a distance of 0.03 and Ba-P2 isolates were related to Bacillus substilis at a distance of 0.01 with an 89% consistency index.
CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC Niruri, Rasmaya; Narayani, Inna; Artama, Wayan Tunas; Astawa, Mantik; Sadewa, Ahmad Hamim
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and PeripheralBlood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for eachtube) were drawn from one adult healthy subject. The samples were categorized into five group beforemeasuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh <GII>and frozen <G-III>] , and PBMC resulted from BC and ficoll isolation [fresh <G-IV> andfrozen <G-V>]. Each group was replicated three times. Blood storage before preparation was less thanfour hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI)was conducted. The mean value of CD4 count (cell /mu1) were 522 (G-I), 1410 (G-II), 906 (G-III), 807(G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G-III) was higher (906 cell /mu1) than PBMC (G-IV) sample (733 cell /mu1).
APPLICATION OF TRICHODERMA SPP. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (PLASMODIOPHORA BRASSICAE WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE Suada, I Ketut; Suwastika, Anak Agung Ngurah Gede; Pradnyana, I Kadek Ngestika; Shchegolkova, Nataliya; Poloskin, Rodion; Gladkov, Oleg; Yakimenko, Olga; Stepanov, Aleksey
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%.  This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.
UTILIZATION OF ELECTRIC PULSED POWER ON FRUITING OF EDIBLE MUSHROOMS Ohga, Shoji
International Journal of Biosciences and Biotechnology Vol 1 No 1 (2012)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Eff ect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes,Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotuseryngii, Pleurotus abalones, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directlycharged to the substrate just before fructifi cation. The eff ect of the pulsed power resulted to promote for 10edible mushrooms fructifi cation. The treatment especially stimulated the fructifi cation on Pleurotus species.
DEVELOPMENT OF IN PLANTA TRANSFORMATION METHOD USING AGROBACTERIUM TUMEFACIENS THAT IS SIMPLE AND EFFICIENT AS WELL AS APPLICABLE TO VARIOUS PLANTS Kojima, Mineo
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

A simple and effi cient in planta transformation method was developed. In the method, meristems of eitherapical or axillary buds of immature plants or apical buds of embryos in imbibed seeds, depending on plants,were inoculated by Agrobacterium tumefaciens after being pricked with a needle. The inoculated plants weregrown to maturation in pots under non-sterile conditions. Transformation was demonstrated by severallines of evidence obtained with mostly the progenies of T1 generation; phenotypic inheritance from T0plants to plants of the following generation, resistance of seed germination to antibiotics, detection of?-glucuronidase activity in transformants of T1 generation, detection of transgene by Southern blot andPCR analyses in T1 generation transformants and rescue from T1 generation transformants of the plasmidscomposed of T-DNA of binary vector and fl anking plant genomic DNA. The diverse species of plants such asbuckwheat (Fagopyrum esculentum), mulberry (Morus alba L.), kenaf (Hibiscus cannabinus), rice (Oryza sativa),wheat (Triticum aestibvum L.), maize (Zea mays), and soybean (Glycine max) were shown to be effi cientlytransformed by our in planta method.
IN VIVO MULTISTEP MUTAGENESIS INDUCTION USING COLCHICINE ON COWPEA MUTANT 1 (VIGNA UNGUICULATA L. WALP) Susrama, I Gede Ketut; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v05.i02.p04

Abstract

In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.
AGARWOOD PRODUCING FUNGAL INOCULANT FORMULATION IN KETIMUNAN TREE (GYRINOPS VERSTEEGII DOMKE) Mega, I Made; Suanda, Dewa Ketut; Kasniari, Desak Nyoman; Susrama, I Gede Ketut
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to find out the agarwood producing fungal inoculant formulation that infect Ketimunan tree (Gyrinops versteegii DOMKE) effectively and resulted in high quality of agarwood. Randomized Completely Block Design was applied in this study with formulation of Fusarium solani and Rhizopus sp inoculant in liquid and solid form were combined as treatments. Treatments were: solid Fusarium solani inoculant, solid Rhizopus sp inoculant, mixture of solid Fusarium solani inoculant and solid Rhizopus sp inoculant, liquid Fusarium solani inoculant, liquid Rhizopus sp inoculant, mixture of liquid Fusarium solani inoculant and liquid Rhizopus sp inoculant, and mixture of solid and liquid of Fusarium solani and Rhizopus sp inoculants. There are three replicates for each treatment. Parameters measured were level of fragrance, agarwood color, and its resin content. The result showed that all formulations affected resin content significantly compared to control of agarwood. Mixture of liquid Fusarium solani and liquid Rhizopus sp inoculant showed the best quality agarwood with quality characteristics: brownish black or agarwood with black and brownish black color, very strong fragrance, and with 13.58% resin content.
CONTENT ARTICLES Articles, Content
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Content Articles
ANALYSIS OF GENE TARGETING EVENTS IN BUCKWHEAT (FAGOPYRUM ESCULENTUM) TRANSFORMED B THE MUTANT STRAINS OF AGROBACTERIUM TUMEFACIENS THAT HAVE DEFICIENCY IN T-­??DNA INTEGRATION STEP INTO PLANT GENOME Kojima, Mineo; Suparthana, Putu; Shimizu, Tsutomu; Nogawa, Masahiro
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Gene targeting events were analyzed in buckwheat (Fagopyrum esculentum, var. Shinano No. 1) that werein planta transformed (T1) by the three strains of Agrobacterium tumefaciens harboring a gene targetingvector for an endogenous gene (acc.no.AB327276); M-­??1 mutant (abvA::Tn5) and M-­??31 mutant (virA::Tn5)strains, both of which are capable of transferring its T-­??DNA into nucleus of host plant cell, but have adeficiency in T-­??DNA integration step into chromosome and LBA4404 strain, being a commonly usedstrain. The results of both phenotype examination and Southern blot analysis implied that a targetingconstruct(s) was integrated into a different locus (loci) each depending on the strain of A. tumefaciens usedfor transformation. Both 5?-­??end and 3?-­??end flanking DNA segments that were expected from preciseinsertion of targeting construct into the endogenous U-­??gene locus were obtained by PCR with 3 (23%)transformants out of the randomly selected 13 transformants by M-­??1 mutant strain, while only 3?-­??endflanking DNA segment was obtained with 3(21%) transformants out of 14 transformants by M-­??31 strain.Taken together, the results suggested the potential of usage of a mutant strain of A. tumefaciens for genetargeting which has a deficiency in T-­??DNA integration step into plant genome.