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BERITA BIOLOGI
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Articles 2,027 Documents
THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

Abstract

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Kusmini, Irin Iriana; Gustiano, Rudhy; Putri, Fera Permata
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Abstract

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.
KARAKTERISASI PROTEASE BACILLUS SUBTILIS A1 INACC B398 YANG DIISOLASI DARI TERASI SAMARINDA Soeka, Yati Sudaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Abstract

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).
SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT Efendy, Oscar; Fathoni, Ahmad; Praptiwi, Praptiwi; Royyani, Mohammad Fathi; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3621

Abstract

Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml.  Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.  
KERAGAMAN DAN KELIMPAHAN LEBAH SOSIAL (APIDAE) PADA BUNGA TANAMAN PERTANIAN MUSIMAN YANG DIAPLIKASI PESTISIDA DI JAWA BARAT Kahono, Sih; Erniwati, Erniwati
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.660

Abstract

Study of diversity and abundance of social bees (Apidae) in the pesticide-applied seasonal crops were carried out for nine species of crops in a lowland area, Dramaga, Bogor and on 17 species of crops ina highland area, Lembang, Bandung, both in the province of West Java. Two species of honey bees (Apis cerana and A. dorsata) and one species of stingless bee (Trigonalaeviceps) were recorded from the lowland crops,while only A. cerana was found in the highland crops. The most abundant bee species on both low and highland was the honey bee A. cerana. The number of A. cerananests and T. laevicepsnests in the surroundings of the study sites were relatively high. Colony members of both bee species were very active to collect nectar and pollen from flowers in non-pesticide-applied sites; however, the density of bees visiting flowers in pesticide-applied seasonal crops in both lowland and highland were relatively low.
LIGHT INTENSITY AND THE SPREAD OF CESTRUM AURANTIACUM LINDL. IN A SECONDARY MOUNTAINOUS REMNANT FOREST Junaedi, Decky Indrawan
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.516

Abstract

Penyebaran Cestrum aurantiacum Lindl. di hutan remnant sekundair Wornojiwo Kebun Raya Cibodas (KRC) dianalisis dalam penelitian ini menggunakan Nearest Neighbor Method. Peranan (korelasi) faktor lingkungan terhadap pola penyebaran C. aurantiacum dibandingkan dengan jenis pohon asli dan dilakukan dengan menggunakan cluster analysis. Faktor-faktor lingkungan yang digunakan dalam cluster analysis ini yaitu: intensitas cahaya, keasaman tanah, kelembapan tanah dan kelembapan udara. Hasil analisis penyebaran menunjukkan bahwa jarak penyebaran C. aurantiacum hampir lima kali lebih besar daripada penyebaran yang diasumsikan terjadi secara acak. Hasil cluster analysis menunjukkan bahwa intensitas cahaya berhubungan secara nyata dengan penyebaran C. aurantiacum di hutan Wornojiwo. Intensitas cahaya rata-rata di plot dengan C. aurantiacum lebih besar dibandingkan dengan plot tanpa C. aurantiacum. Contoh pengaplikasian hasil penelitian ini adalah memprioritaskan eradikasi C. aurantiacum yang ada di pinggir hutan daripada yang ada di bagian tengah. Hasil penelitian ini juga mempertegas pentingnya memelihara tegakan hutan alami untuk memelihara naungan hutan.
LUMUT SEJATI DI KAWASAN CAGAR ALAM GUNUNG PAPANDAYAN GARUT, JAWA BARAT Windadri, Florentina Indah
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.675

Abstract

Research on moss diversity in the Papandayan Nature Reserve has been conducted covering the western and eastern areas of the Nature Reserve. Fifty one species of mosses from 14 families and 30 genera were recorded and collected with Dicranaceae as the dominant family.Four species of mosses (Papillaria crocea (Hampe) Jaeg., Barbella rufifolioides (Broth.) Broth., Ctenidium luzonense Broth. and Trichostomum brachydontium Bruch ex Mull.Hal are reported here as new records to Java.
IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER PANULIRUS HOMARUS Sudewi, Sudewi; Widiastuti, Zeny; Mastuti, Indah; Mahardika, Ketut
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3578

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.   
KEANEKARAGAMAN KOMUNITAS KRUSTASEA DI KEPULAUAN MATASIRI KALIMANTAN SELATAN Pratiwi, Rianta; Wijaya, Nirmalasari Idha
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.525

Abstract

Studies on the community diversity of crustaceans have been conducted in the waters of Matasiri Islands, South Kalimantan, from 19th November to 1st December 2010. This research was conducted to determine the density, diversity and the presence of crustacean fauna in the waters of South Kalimantan, especially in the Matasiri Islands. Samples were collected using trawl gear operated by KR (Research Ship) BarunaJaya VIII in four Stations and three Stations of free collecting along the islands. Free sampling was conducted along the beach and the reef edge by breaking live and dead rocks, and digging sand and mud in the mangrove or using hand net around the beach of Matasiri Islands. Collection is also done using the gillnet gear which is installed in shallow water during high tides about 4 hours.The samples were collected during low tide. The results obtained were 1882 individuals covering 86 species and 19 families. The diversity index ranges between 0.97 (the lowest at Station 3 of free collecting) and 3.74 (the highest in Station 3 of trawl). While the similarity index ranged from 0.36 (the lowest in Station 1 of trawl) to 0.97 (the highest in Station 3 of free collecting). Penaeidae prawn has the highest density in each observed station. Portunidae crab has the second highest density. South Kalimantan waters especially Matasiri Islands is still in good condition for crustacean life.
KARAKTERISASI REPRODUKSI DAN NILAI HETEROSIS HASIL PERSILANGAN IKAN GURAME BASTAR DAN BLUESAFIR Radona, Deni; Nafiqoh, Nunak
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.689

Abstract

Hybridization is one alternative of genetic improvement to increase the production of commercialize commodity. The aim of this study was to determine the character of reproductive performance and the value of heterosis from cross-breeding of giant gouramy (Osphronemus gouramy) strain Bastar and Bluesafir. Crosses were performed in both directions (reciprocal) with naturally spawning system. Two pairs of giant gouramy of each strain were used, each pair contained 4 ? and 1 ?. Every pairs were reared in 3 x 3 m of pond.Reproductive characterization, such as Fecundity, Ovo Somatic Index, egg diameter, egg weight, Fertilization Rate (FR), Hatching Rate (HR), the latency time of hatching, and yolk sack absorbtion period, were observed. Other parameters including larval lenght and Survival Rate (SR) were also observed. Heterosis (H) value was calculated from FR, HR and SR. Result showed that each population and its cross-breed had no different in reproductive characters. Latency time of hatching, egg diameter, egg weight and yolk sac absorbtion period values were 3 ± 0 days; 0.74 ± 0.05 cm; 11 ± 4 mg dan 9 ± 1 days, respectively. Meanwhile fecundity value showed that Blusafir strain had higher fecundity (3661 ± 624), and Ovo Somatic Index percentage (21.96 ± 3.74). The highest HR was presented by ? Bluesafir x ? Bastar (97.95 ± 0.47 %), meanwhile ? Bastar x ? Bluesafir presented the highest FR and SR (99.15 ± 0.34 % and 92.57 ± 0.40 %). Heterosis value showed that cross-breeding between ? Bastar x ? Bluesafir had better HR and SR (1.20 and 2.37), white hand cross-breeding between ? Bluesafir x ? Bastar presented better FR (0.69).

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