Claim Missing Document
Check
Articles

Found 5 Documents
Search

HUBUNGAN POLIMORFISME GEN ACTN3 DENGAN KELINCAHAN, DAYA LEDAK, DAN KECEPATAN candrawati, susiana; Gumilas, Nur Signa Aini; Kusuma, Muhammad Nanang Himawan; Adiningtyas, Pradani Eva; Sucipto, Muhammad Cahya Riadi; Rahmah, Sarah Shafira Aulia
Jurnal Kedokteran Brawijaya Vol 29, No. 4 (2017)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.091 KB) | DOI: 10.21776/ub.jkb.2017.029.04.8

Abstract

Komponen biomotorik kelincahan, daya ledak, dan kecepatan merupakan komponen yang harus dimiliki atlet sepak bola. Penelusuran genetik dapat menjadi salah satu metode talent profiling pada atlet sepak bola. Talent profiling belum pernah dilakukan pada kalangan atlet Indonesia terutama atlet yunior seperti atlet Unit Kegiatan Mahasiswa (UKM) Sepak Bola di (Universitas Jenderal Soedirman) UNSOED. Salah satu gen yang berhubungan dengan performa adalah gen ACTN3. Gen ACTN3 yang merupakan gen pengkode protein ?-aktinin-3 pada serabut otot tipe cepat, diduga berpengaruh terhadap kelincahan, daya ledak dan kecepatan. Tujuan penelitian ini adalah untuk mengetahui hubungan polimorfisme gen ACTN3 dengan kelincahan, daya ledak dan kecepatan otot pada atlet sepakbola. Penelitian ini menggunakan rancangan studi observasional analitik dengan pendekatan cross sectional. Metode pengumpulan subjek pada penelitian ini menggunakan consecutive sampling. Subjek pada penelitian ini adalah 82 atlet yang mengikuti UKM sepak bola Universitas Jenderal Soedirman. Metode PCR-RFLP dan elektroforesis digunakan untuk melihat polimorfisme gen ACTN3. Sedangkan pengukuran komponen biomotorik menggunakan tes agilitas Illinois (kelincahan), jump meter digital (daya ledak) dan tes sprint 30 meter (kecepatan). Analisis data menggunakan uji ANOVA satu arah dengan tingkat kemaknaan p=0,05. Terdapat hubungan bermakna antara polimorfisme gen ACTN3 dengan daya ledak otot (p=0,027) dan tidak terdapat hubungan bermakna antara polimorfisme gen ACTN 3 dengan kelincahan (p=0,084) dan kecepatan (p=0,507). Rerata terbaik ketiga komponen biomotorik terdapat pada alel RR gen ACTN 3. Disimpulkan terdapat hubungan antara polimorfisme gen ACTN3 dengan daya ledak otot, tapi tidak terdapat hubungan antara polimorfisme gen ACTN 3 dengan kelincahan dan kecepatan pada atlet sepakbola.
FAKTOR-FAKTOR YANG MEMPENGARUHI TINGKAT KEPATUHAN DIET PADA PASIEN DIABETES MELITUS TIPE 2 DI KECAMATAN SUMBANG BANYUMAS Ernawati, Dwi Arini; Harini, Ika Murti; Gumilas, Nur Signa Aini
Journal of Bionursing Vol 2, No 1 (2020)
Publisher : Fakultas Ilmu-ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/bion.v2i1.40

Abstract

Background problem: Diabetes Mellitus (DM) prevalence in Indonesia based on the results of basic health Research (RISKESDAS) year 2013 is 2.1%, in Central Java  18.33%. Banyumas District has an incidence rate of 1,417 in 2014 and Sumbang Sub-district has the highest incidence rate of DM type 2. Management in DM sufferers is integrated, including eating arrangements, physical activity, education, and medicines. Meal arrangement for patients with type 2 DM includes setting against 3J: number, type and meal schedule in a day. Patients with DM who have already run a diet program has not been able to control blood glucose properly. Food arrangement often fails due to the disobedience of sufferers in running their diet. Many factors affect dietary compliance, among them the level of education, level of knowledge, self-motivation, family support and the role of health workers.Objective: To determine the factors that affect the compliance of diet in diabetes mellitus type 2 patients in Sumbang Sub-district of Banyumas.Methods: The studies used are cross sectional, the level of compliance seen from 3J, i.e. precise amounts, precise types and precise schedules. Samples taken from DM patients who are in Prolanis present during laboratory examination in June 2019. Sampling technique using total sampling. Bound variables are the level of dietary compliance, the free variables are education levels, knowledge levels, self-motivation, family support and the role of health workers.Results: The number of samples was 32 people, who were obedient in a diet of 15 people (46.9%), who did not obey 17 people (53.1%). The P value of the factor variable factors affecting the diet is: age p = 0.411, sex p = 0.035, Job p = 0131, long DM p = 0.261, education level p = 0.035, knowledge level p = 0.036, self motivation p = 0.464, family support p = 0.314, and P Health worker, p = 0.452. Variables that have a meaningful relationship (P < 0.05) with dietary compliance levels are gender, level of education and level of knowledge. As for the variable age, employment, old DM, self-motivation, family support, and the role of health workers have an insignificant relationship (P > 0.05Conclusion: From 32 patients DM most have a level of diet compliance in the category of disobedient 17 people (53.1%), there is a meaningful relationship between gender, education level and level of knowledge with DM dietary compliance,
REPRODUCTIVE PARAMETERS OF MALE ALBINO RATS AFTER INDUCTION IN SLEEP DEPRIVATION MODELS Arjadi, Fitranto; Harini, Ika Murti; Gumilas, Nur Signa Aini
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Proceeding International Seminar of Occupational Health and Medical Sciences (I-SOCMED) 2017 â
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Paradoxical sleep deprivation (PSD) and total sleep deprivation (TSD) cause disrupt of male infertilitybut sleep recovery (SR) can improve male reproduction function that connected with occupational works health. The aim of this study is to determine the difference of reproduction parameters in male albinorats  after exposed by various sleep deprivation models. This research was experimental post-test only with control group design. Rats were divided into 5 groups (6 animals each group) : negativecontrol, PSD (II), TSD (III), PSD with SR, TSD with SR.  The study results  showed that average of spermatogenic  group IV (8.35 ± 0.06) and V (8.27 ± 0.27) had higher scores. group IV had the highest number of Leydig cell (5,91 ± 1,43),  group I had the highest rates (40,02 ± 2,04)   number of Sertoli cell and there were no significant difference  average of epithelial diameter (p=0,598) and height (p=0,895). There were significant difference of spermatogenic score post-SR,  number of Sertoli and Leydig cells, but no significant difference of epithelial diameter and height of seminiferous tubule after exposed by various sleep deprivation stress model. Sleep recovery in occupational work can  repair the histological parameter of reproduction. Key word : male albino rats, reproduction parameters,  sleep deprivation models
Comparison of the Effect of Ethanol Extracts of Phaleria macrocarpa (scheff.Boerl) Fruit and Metformin on the Kidney Function of Hyperglycaemic Rat Models sutrisna, Eman; Sutrisna, Eman; Gumilas, Nur Signa Aini; topa, Mus; Sulistyoningrum, Evy
Herb-Medicine Journal Vol 3, No 2 (2020): Herb-Medicine Journal Juli 2020
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/hmj.v3i2.6656

Abstract

Diabetes mellitus is a disease that often causes diabetic nephropathy complications due to persistent hyperglycaemia. Phaleria macrocarpa (scheff. Boerl)  is one of the plants that has been widely used in the treatment of diabetes mellitus, but its effect on the risk of diabetic nephropathy is still unknown. A dose of 300mg / 200gbb / day is known as an effective dose that can significantly reduce blood sugar levels in diabetic rat. The Streptozotocin Effect (STZ) causes damage to the pancreas and causes hyperglycaemia. This study aims to compare the effect of Phaleria macrocarpa (scheff. Boerl) fruit extracts on urea and creatinine levels as a parameter of kidney function in hyperglycaemic mouse models. The study was carried out experimentally using the post-test only with control group design. Fifteen white rats were divided into 3 groups. All rats were induced with 40 mg / 200gbb of Streptozotocin (STZ) to experience hyperglycaemia. Group I as negative control was given aquades. Group II was given ethanol extract of Phaleria macrocarpa (scheff. Boerl) with a dose of 300 mg / 200gbw / day. Group III was given Metformin at a dose of 150 mg / 200gbw / day. On the 22nd day of treatment, blood specimens were taken for examination of urea and creatinine levels. The research data were statistically analyzed with α <0.05; CI95%. The mean of urea levels in Group I (79.80 ± 25.09 mg / dl), II (76.00 ± 22.59 mg / dl and III (59.60 ± 6.35 mg / dl). Kruskal-Wallis test results showed no significant difference in urea levels between treatment groups (p value = 0.273; CI95%). The mean of creatinine levels in Group I (0.68 ± 0.07 mg / dl), II (0.63 ± 0.14 md / dL) and III (0.98 ± 0.25 mg / dL). One Way Anova and Post hoc test results showed a significant difference in mean creatinine levels between Groups I and III (p = 0.014; 95% CI) and II with III ( p value = 0.006; CI95%). the results of this study can be concluded that the extract of Phaleria macrocarpa (scheff. Boerl) fruit flesh at a dose of 300 mg / 200gbb has better effectiveness than metformin dose 150 mg / 200gbb in repairing the kidney function of hyperglycaemia rats.
The Comparative Effectiveness of Phaleria macrocarpa (Scheff.) Boerl. Pericarp Ethanol Extract and Metformin in Reducing Blood Sugar Levels and Ameliorating the Langerhans Islets Degeneration in Diabetic Rat Models Sutrisna, Eman; Aini, Qurrotu; Harini, Ika Murti; Gumilas, Nur Signa Aini; Mustofa, Mustofa; Sulistyoningrum, Evy
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 17 No. 01 Juli 2020
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v17i1.6526

Abstract

Phaleria macrocarpa (Scheff.) Boerl. has been widely used in the treatment of diabetes mellitus but its effectiveness is still inconclusive so it needs to be compared with standard drugs such as metformin. This study aims to analyze the effects of P. macrocarpa fruit extracts on blood sugar levels and the repairing of the pancreatic Langerhans islets of diabetic rat models. The study was conducted experimentally by pre and post-test design with a control group. Thirty white rats were divided into 6 groups. All rats were treated with 40 mg/200 g BW of streptozotocin (STZ) to induce hyperglycemia, except group I. Group II as a negative control was given with aquadest. Group III, IV, and V were given with ethanol extract of P. macrocarpa pericarps at doses of 200, 250, and 300 mg/200 g BW/day, respectively. Group VI was given with metformin at a dose of 150 mg/200 g BW/day. On the 22nd day of treatment, blood specimens were taken for examination of fasting blood sugar levels and pancreatic organs were analyzed for histopathological examination of the islets of pancreatic Langerhans. The data were statistically analyzed with α<0.05; CI95%. Paired sample t-test results showed a significant decrease in blood sugar levels before and after treatment in group V (p-value=0.048). One way anova and post hoc Least Significant Difference (LSD) test results showed a significant difference in mean blood sugar levels after the final treatment between groups I and II (p-value=0.013), I and III (p-value=0.004), I and IV (p-value=0.002), I and VI (p-value=0.000), II and VI (p-value=0.031), and V with VI (p-value=0.005); one way anova and post hoc LSD test results showed a significant difference in the mean area of islets of pancreatic Langerhans between group I and all treatment groups (p-value=0.000), II with V and VI (p-value=0.000 and 0.009), III with V and VI (p-value=0.000 and 0.001), and IV with V (p-value=0.003). It can be concluded that the extract of P. macrocarpa pericarps showed a hypoglycemic effect and repaired the damage in Langerhans islets of STZ-induced rat in a dose-dependent manner.