Claim Missing Document
Check
Articles

Found 4 Documents
Search

IDENTIFIKASI ES CHERICHIA COLI PADA CINCAU YANG DIJUAL DI PASAR BARU STABAT Sapitri, Alfi; Afrinasari, Intan
Journal of Pharmaceutical And Sciences Vol 2 No 2 (2019): JPS Volume 2 Nomor 2 (2019)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v2i2.23

Abstract

Escherichia coli found in human and animal intestine. These bacteria easily spread by contaminated water and food. It can happen on grass jelly (Mesona palustris), which has a high carbohydrate, fiber, and nutrients. Some people use a mixture of grass jelly drinks as well as traditional medicine. This research intended to identify bacterial contamination E. coli of grass jelly sold at Pasar Baru Stabat. The samples in this study are the grass jelly derived from 5 merchants. This research is a descriptive study. The research method was MPN (Most Probable Number) for calculating the coliform bacteria, followed by examination of Lactose Broth media, Brilliant Green Lactose Broth media, Eosin Methylene Blue Agar media, gram stains, and biochemistry test. The results of this study indicated five samples tested, and there is one positive sample contaminated with bacteria E. Coli a four negative samples contaminated with E. coli. So that four samples were suitable for consumption, and one sample was not suitable for consumption.
Uji Daya Hambat Sediaan Sampo Air Perasan Jeruk Purut (Citrus hystrix) Terhadap Pertumbuhan Microsporum gypseum Maimunah, Siti; Zega, Rita Marthalena; sapitri, Alfi
Journal of Pharmaceutical And Sciences Vol 2 No 2 (2019): JPS Volume 2 Nomor 2 (2019)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v2i2.29

Abstract

The shampoo is a dosage material used as a hair cleanser and epicarp head, especially the problem of dandruff caused by fungus. Kaffir lime has the content of essential oils, flavonoids and tannins are efficacious as an antifungal. The purpose of this study was to test the shampoo of kaffir lime and to know its inhibitory power to growth of Microsporum gypseum fungus. The type of this research is experiment with using agar diffusion method. Samples studied were epicarp juice of lime and mesocarp of kaffir lime with concentration respectively 10%, 20%, 30%, 40%, 50%. Shampoo dosage evaluation tests include inhibitory test, organoleptic test, pH test, high foam test and wetting test. The results showed that the lime mesocarp juice had inhibitory at 10% (13,09 mm) concentration, 20% (14,9 mm) concentration, 30% (18,52 mm) concentration, 40% concentration (20,86 mm ) and a concentration of 50% (22.53 mm). While the results of epicarp water of lime juice have a weak inhibitory power with a concentration of 10% (5,66 mm), Concentration of 20% (7,09 mm), concentration of 30% (8,09 mm), concentration of 40% (10,19), and concentration of 50% (11,36). Effective concentration on the preparation of shampoo preparations is the concentration of 20% and 30% of the lime juice mesocarp juice. The results of the evaluation test of two anti-dandruff shampoo formulations meet the requirements of a good shampoo and have respective inhabitants of F1 (20%) has a resistance of 14,54 mm and F2 (30%) has a resistance of 19,12 mm. Both dosage preparations are anti-dandruff.
Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus Sapitri, Alfi; Lara, Nofita; Sitorus, Panal
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2 (2020): Jurnal Pembelajaran Dan Biologi Nukleus
Publisher : Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1766

Abstract

Senduduk (Melastoma malabathricum L.) is one of the wild plants used as traditional medicine. Senduduk leaves are used as a medicine for sprue, boils, diarrhea and smallpox. The purpose of this study was to determine the antibacterial activity of the ethanol extract of senduduk leaves of the against Escherichia coli and Staphylococcus aureus. Senduduk leaves extracted by maceration using 96% ethanol solvent. Antibacterial activity testing is carried out using the diffusion method using paper discs.This experiment was repeated three times using concentrations of 20%, 40%, 60% and 80%.Ethanol extract of the senduduk leaves contains flavonoids, saponins, tannins and steroids/triterpenoids. The test results showed that the ethanol extract of the leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus which is characterized by the inhibition zone formed around the disc paper. In Escherichia coli bacteria the smallest inhibition zone at a concentration of 20% with a diameter of inhibition zone 11 mm and the largest diameter of the inhibitory zone at a concentration of 80% with a diameter of inhibition zone 19 mm. whereas for the smallest inhibitory zone Staphylococcus aureus bacteria at a concentration of 20% with an inhibition zone diameter of 12.6 mm and the largest inhibitory zone dameter at a concentration of 80% with a diameter of the inhibitory zone 21,3 mm.The results of the study concluded that the ethanol extract of Senduduk leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus.
THE ABILITY OF RED CHILI (Capsicum annum L.) ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIA Staphylococcus aureus and Escherichia coli Sapitri, Alfi; Marbun, Eva Diansari; Mayasari, Ulfayani
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.39859

Abstract

Red chili (Capsicum annum L.) has empirically effective as an antioxidant and antibacterial because it contains capsaicin compounds. This study aims to determine the antibacterial activity of the ethanol extract of red chili (Capsicum annum L.) against Staphylococcus aureus and Escherichia coli and the chemical compounds contained in red chilies. Testing for antibacterial activity using the agar diffusion method using disc paper with three repetitions. The ethanol extract of red chili is made with a concentration of 45%, 60%, 75% and 90%. The medium used as an antibacterial test was Mueller-Hinton Agar (MHA). The results showed that the ethanol extract of red chili (Capsicum annum L.) contained compounds containing secondary metabolites, namely alkaloids, flavonoids, saponins, tannins and steroids / triterpenoids. The result of antibacterial activity was an inhibition zone formed around the disc paper in the ethanol extract of red chili against Staphylococcus aureus bacteria with a concentration of 45%, 60%, 75%, 90%, each of which had an inhibition zone of 8.7 mm, 10.8 mm. 13.2 mm and 16.9 mm. Meanwhile, Escherichia coli with a concentration of 45%, 60%, 75%, 90%, respectively, have an inhibition zone of 9.3 mm, 10.4 mm, 12.1 mm and 15.6 mm.