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Skrining Aktivitas Antibakteri Fraksi Kelopak Bunga Mussaenda frondosa L. Efendi, M. Rifqi
Journal of Pharmaceutical And Sciences Vol 2 No 1 (2019): JPS Volume 2 Nomor 1 (2019)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v2i1.18

Abstract

Background: Antibiotics are the most effective drugs against microbial infections, but slowly lose their efficacy to microorganisms due to resistance. So, nowadays, research related to finding new compounds from nature to treat infectious diseases has become important. Mussaenda frondosa L. is a tropical plant native to Indonesia. Traditionally, Mussaenda frondosa L. has been used for the treatment of various diseases, such as headaches and jaundice. The literature study of the antibacterial activity of Mussaenda frondosa L. is still very limited, so research needs to be done regarding the antibacterial activity. Objective: to determine whether there is antibacterial activity from Mussaenda frondosa L. petal fraction. And whether there is significant antibacterial activity when it is compared to chloramphenicol as positive control antibiotic. Method: The methodology of this research starts with sampling, sample identification, extraction, and fractionation. Screening for antibacterial activity was carried out by the agar diffusion method. Results: The most potential antibacterial activity was ethyl acetate fraction with an inhibitory diameter of 5.6 - 8.3 mm against 12 test pathogenic bacteria, except Streptococcus mutans ATCC 25175. The n-hexane fraction showed antibacterial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Vibrio cholerae inaba with an inhibitory diameter of 6.0 - 7.5 mm. Meanwhile, the n-butanol fraction has only antibacterial activity against Staphylococcus aureus ATCC 25923 with a 7.5 mm inhibition diameter. Conclusion: the most potential fraction that had antibacterial activity was ethyl acetate fraction, by inhibiting 12 test pathogenic bacterias, followed by n-hexane fraction which inhibits 3 bacterias.
Isolation and Antibacterial Activity Test of The Extract Ethyl Acetate of Endophytic Fungi from Kencur (Kaempferia Galanga L.) Efendi, M. Rifqi; Rusdi, Mesa Sukmadani; Anisa, Fitria
Journal of Pharmaceutical And Sciences Vol 3 No 2 (2020): JPS Volume 3 Nomor 2 (2020)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v3i2.42

Abstract

The aim of this research was to isolate the endophytic fungi found in leaves and rhizomes of Kaempferia galanga L. and to test its antibacterial activity. Isolation of endophytic fungi is carried out by cultivating and sub-cultivating fungi that found in leaves and rhizomes of Kaempferia galanga L. Identification of endophytic fungi is done by its morphology macroscopically and microscopically. Antibacterial activity testing is carried out by using the agar diffusion method. From the leaves and rhizome of Kaempferia galanga L., five isolates of endophytic fungi were identified as Torulla sp. (KG001), Mucor sp. (KG002), Fusarium sp. (KG003), Geotricum sp. (KG004), and Drechslera sp. (KG005). Three ethyl acetate extracts of the endophytic fungi Torulla sp. (KG001), Fusarium sp. (KG003), and Drechcera sp. (KG005) had antibacterial activity against Gram positive (Staphylococcus aureus and Vibrio cholera) and Gram negative bacterias (Bacillus subtilis and Eschericia coli) at concentration of 3.75%. The ethyl acetate extract endophytic fungi that had strong antibacterial activity (10-20 mm in diameter) were Torulla sp. (KG001) and Drechslera sp. (KG005). Meanwhile, the endophytic fungi that had moderate antibacterial activity (inhibition diameter 5-10 mm) was the ethyl acetate extract Fusarium sp. (KG003).The strongest antibacterial activity was shown by the ethyl acetate extract Drechslera Sp. with an inhibitory diameter 16 mm against Vibrio cholera .
Teratogenic Effects of Durian Fruit (Durio Zibethinus Murr) on White Mice Fetus (Mus musculus L) Rusdi, Mesa Sukmadani; Efendi, M. Rifqi; Hilma, Hafizhatul
Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan Vol 7, No 1 (2021): Pharmauho
Publisher : Fakultas Farmasi Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/pharmauho.v7i1.13489

Abstract

Durian (Durio zibethinus L.) is one of the most consumed and liked fruit in Indonesia, but there was no evidence about the safety of consuming durian during pregnancy. This study was determine the level of safety of durian fruit consumption during pregnancy by conducted teratogenicity test of durian fruit (Durio zibethinus Linn) on fetuses of white mice (Mus musculus L). Teratogenicity test was conducted in vivo, by step: preparation of experimental animal, observation during administration, laparotomy, fixation and observation of morphological defects. Data were analyzed using Analysis of Variance (ANOVA). If the results were significant (P < 0.05), the analysis was continued using Duncan Multiple Range Test. Administration durian during pregnancy did not affects the bodyweight of the mice (P > 0,05), but significantly influences the number of fetuses (P < 0,05) and its body weight (P < 0,05). Consumption of durian during pregnancy is safe in 32,5 mg / KgBW, but it has the potential teratogenic in 65 mg / KgBW and 97,5 mg/KgBW groups. Teratogen effects that were found in several fetuses were underweight, dead, hemorrhage, maternal death and there were resorption sites.